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METODOLOGI

PENELITIAN BIOMEDIS

Eko Prase(B351150

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SEROPREVALENSI Q FEVER PADA SAPI DI KABUPATEN BOYOLALI

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LATA! BELA"ANG

#oo$os%s Pe$yak%t ya$g &a'at &%tuarka$ &ar% he)a$ ke hehe)a$ ke +a$us%a* &a$ ,uga se-a%k$ya

Pe$yak%t he)a$ +e$uar strateg%s "e'+e$ta$ No./06"'ts.OT.1/03013  ,e$%s

Da2tar 'e$yak%t ya$g su&ah a&a &% I$&o$es%a +au'u$ ya$g -a&a &% I$&o$es%a -er'ote$s% +e$%+-uka$ kerugian ekonomengganggu kesehatan manusia an !ingkungan serta+e$%+-uka$ keresahan mas"arakat

#$%EVER

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Q-FEVER

 Pe$yak%t oo$os%s 'ote$s%a  age$ 'e$ye-a- -akter% Coxieburnetii

 Ge,aa k%$%s (Set%yo$o 0054

a. Pa&a he)a$ su-k%$%s  t%&ak +e$u$,ukka$ ge,aa s'es%

su%t &%%&e$t%kas%-. Pa&a +a$us%a  fu like symptom, sesak $a2as* '$eu+o$%a

. ara 'e$uara$ 4 aeroso* ko$tak a$gsu$g* ko$tak t%&ak a$

. Beu+ a&a a'ora$ ke,a&%a$ 782e9er -a%k 'a&a he)a$ +au+a$us%a &% I$&o$es%a

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PE!:M:SAN MASALA;

 Data 're9ae$s% 'e$yak%t Q ever &% I$&o$es%a +as%h ter-ata

 Stu&% +e$ge$a% 'e$yak%t Q ever 'a&a sa'% 'erah &% I$&o$e-eu+ 'er$ah &%a'orka$

 "a-u'ate$ Boyoa% se-aga% saah satu se$tra sa'% 'oto$g &

sa'% 'erah &%

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LAN

 Su+-er 'e$uar 782e9er  sa'% %+'or (Sa'% B? asa Austraasa 782e9er

 D% I$&o$es%a ter&a'at !P; ya$g +e+oto$g sa'% %+'or 1/100 sa+'e orga$ ya$g &%a+-% &ar% !P; &% Bogor &a$ %-%$ose=ara %+u$oh%stok%+%a 'os%t%2 (Set%yo$o 01/@ &%

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LAN

 Pe$e$tua$ okas% ya$g 'ote$s%a  "a-u'ate$ Boyoa%  Bse-aga% satu &%a$tara ka-u'ate$ &%

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 T:

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MANAAT PENELITIAN

 Ma$2aat ya$g &%hara'ka$ &ar% 'e$e%t%a$ %$% a$tara a%$4

1. Da'at +e+-er%ka$ %$2or+as% +e$ge$a% sero're9ae$s% 'e$Q ever 'a&a sa'% 'erah &% "a-u'ate$ Boyoa%

. Se-aga% u'aya 'e+etaa$ (+a''%$g ke,a&%a$ Q ever &%I$&o$es%a se=ara u+u+

3. Me$,a&% a$gkah a)a u$tuk &%akuka$$ya t%$&aka$ 're9e$'e$ye-ara$ &a$ 'e$uara$ 'e$yak%t Q ever &aa+ =aku'a$ ye-%h uas.

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!:ANG LING":P PENELITIA ;a$ya u$tuk +e$getahu% 're9ae$s% ke,a&%a$ Q ever 'a&a sa'% 'erah &% "a-u'ate$ Perta+a* ha ya$g %$g%$ &%ketahu% a&aah ke,a&%a$ Q ever se=ara e'%&e+%oog% &e$ga+e%hat 're9ae$s% 'a&a sa'% 'oto$g ya$g &%se+-e%h &% !P; A+'e "a-u'ate$ Boyoa

A&a'u$ ,e$%s sa'% ya$g &%se+-e%h a$tara a%$ sa'% oka &a$ sa'% %+'or ya$g -%asa &%&% !P; terse-ut. Sa+'e ya$g aka$ &%a+-% a&aah ha$ya sa+'e %+'a &a$ ,a$tu$g &

sa'% ya$g &%se+-e%h &% !P; &%+aksu&.

Sea$,ut$ya ha %$g%$ &%a+at% a&aah 're9ae$s% Q ever 'a&a sa'% 'erah. Pe+%%ha$ osa+'%$g &%akuka$ &e$ga$ se$ga,a -er&asarka$ &ata B"PM (01.

 

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"E!ANG"A PEMI"I!AN DAN;IPOTESIS Aur "era$gka Pe+%k%ra$ Pe$e%t%a$

 Sesua% &e$ga$ tu,ua$ 'e$e%t%a$ ya$g teah &%ru+uska$ &a'&%2or+uas%ka$ -e-era'a h%'otes%s se-aga% -er%kut4

"a-u'ate$ Boyoa% se=ara stu&% e'%&e+%oog% 'os%t%2 Q eve

"e,a&%a$ 'e$yak%t Q ever ter,a&% 'a&a sa'% 'erah &% "a-u'Boyoa%

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MATE!I DAN METODE

 Tem&at an 'aktu Pene!itian. Pe$e%t%a$ &%akuka$ &% La-orator%u+ Ter'a&u* akutas "e&oktera$ ;e)a$I$st%tut Perta$%a$ Bogor 'a&a -ua$ Se'te+-er 016 sa+'a% &e$ga$ Se'01.

. Sa+'e 'e$e%t%a$ -eru'a sa+'e orga$ %+'a &a$ ,a$tu$g &ar% 50 ekor s'oto$g &% !P; A+'e "a-u'ate$ Boyoa% serta sa+'e &arah ya$g &%a+-

10 ekor sa'% 'erah &ar% set%a' ke=a+ata$ ter'%%h.. "e=a+ata$ ya$g &%'%%h a$tara a%$ e'ogo* Boyoa%* Musuk* Mo,oso$go* &a$ A+'e. "e=a+ata$ terse-ut ter'%%h -er&asarka$ B"PM (01 -ah)a+asyarakat$ya sea%$ -erter$ak sa'% 'erah ,uga +e+e%hara sa'% 'oto$g

. Sa+'e &arah ya$g &%a+-% a&aah se-a$yak 5 +L &ar% set%a' sa'% 'erah ter'%%h.

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METODE PENELITIAN

  TA;AP 1

Sa+'eOrga$

Past%k-er2or+a%

$

Ekstraks%DNA

Neste&P!

A$a%s%s;as%

A+'%kas%

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LAN

Sa'% 'erahter'%%h

De$ga$ s'o%ta+-% &arah

9%a 9e$a 

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P:STA"A

FB"PM Ba&a$ "oor&%$as% Pe$a$a+a$ Mo&a. 01. "a-Boyoa%.

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 TE!IMA "ASI;