human myeloid cells from peripheral blood - miltenyi biotec · 2018-02-01 · miltenyibiotec.com...

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StraightFrom® Whole Blood CD14 MicroBeads

StraightFrom® Buffy Coat CD14 MicroBead Kit

StraightFrom® LRSC CD14 MicroBeads

CD16 MicroBeads

Legend

separation differentiation (cultivation)

MACS® Cell Separation products (RUO) MACS Cell Culture and Stimulation products (RUO) MACS Flow Cytometry products (RUO)

DC: dendritic cellcDC: conventional or myeloid DCpDC: plasmacytoid DCMo-DC: monocyte-derived DCPBMC: peripheral blood mononuclear cell

− no expressionlow low expressionint intermediate expression + expression

high high expression+/− expression dependent

on subset

StraightFrom Whole Blood CD15 MicroBeadsStraightFrom Whole Blood CD66b MicroBeads

CD141 (BDCA-3) MicroBead Kit

Myeloid DC Isolation Kit

CD11b MicroBeads

M-CSF/IL-34 + RANKL GM-CSF/IFN-γ + LPS M-CSF

IL-4/IL-13 IC + TLR-Ligands IL-10/TGF-β

IL-4/GM-CSF

CD14 MicroBeadsCD14 MicroBeads UltraPure

Classical Monocyte Isolation Kit

Pan Monocyte Isolation Kit

CD16+ MonocyteIsolation Kit

MACSxpress® NeutrophilIsolation Kit

MACSxpress Eosinophil Isolation Kit

CD1c (BDCA-1) DC Isolation Kit

Myeloid DC Isolation Kit

Diamond Plasmacytoid DC Isolation Kit IIPlasmacytoid DC Isolation Kit IICD304 (BDCA-4/Neuropilin-1)MicroBead Kit

Blood DC Isolation Kit II

Pan-DC Enrichment Kit

Slan (M-DC8)Monocyte Isolation Kit

CD15 MicroBeads

HLA-DR+

MicroBeads +CD14 MicroBeads

Basophil Isolation Kit

Diamond BasophilIsolation Kit

CD123 MicroBeads

Eosinophils and Neutrophils

Neutrophils Eosinophils

CD15+

CD16+

CD66b+

CD177+/−

CD45+

CD14+/−

CD15+/−

HLA-DR+

CD3+/−

CD19+/−

CD56+/−

CD15+

CD66b+

CD11b+

CD15+

CD16+

CD66b+

CD177+/−

CD181 (CXCR1)+

CD182 (CXCR2)+

CD15+

CD16−

Siglec-8+

CD66b+

CD193 (CCR3)+

BasophilsG-MDSCspDCs Pan DCs Slan (M-DC8) DCsCD141+ cDCs

HLA-DR+

CD141 (BDCA-3)+

CD370 (CLEC9A)+

XCR1+

CD123 (IL-3R)−

CD11c+

CD11blow

HLA-DR+

CD303 (BDCA-2)+

CD304 (BDCA-4)+

CD123 (IL-3R)high

CD85g (ILT7)+

CD11clow

CD11c+/−

CD14+/−

CD16+/−

CD33+

CD1c+ cDCs

HLA-DR+

CD172a (SIRPα)+

CD1c (BDCA-1)+

CD11b+

CD123 (IL-3R)int

CD11chigh

CD15+

CD16−

Siglec-8+

CD66b+

CD193 (CCR3)+

EosinophilsNeutrophils

Pan monocytes Non-classicalmonocytes

CD14high

CD16−

CD15−

HLA-DR+

CD14+/int

CD16+/−

CD15−

HLA-DR+

CD14int

CD16high

CD15−

HLA-DR+

Classicalmonocytes

HLA-DR+

CD3−

CD14−

CD19−

CD56−

HLA-DR+

Slan (M-DC8)+

CD11c+

CD14int

CD16high

CD11bdim

FcεRI+

CX3CR1high

CD115+

CD15+

CD66b+

LOX-1+

CD11b+

HLA-DRlow

CD14+

CD33+

CD11b+

CD15−

CD203c+

CD123+

aIgE+

M-MDSCs Myeloid cells (mix)

CD14−

CD11b+

CD209 (DC-SIGN)high

HLA-DR+

HLA-DR+

CD80+

CD86+

Cathepsin K+ MMP-9+

Osteoclasts Mo-macrophages(M1-like)

HLA-DR+

CD86+

iNOS+

Mo-macrophages(M2b-like)

CD163+

CD206+

Arg1+

Mo-macrophages (M2a-like)

CD206+

SLAM+

Arg1+

Mo-macrophages(M2c-like)

Mo-DCs

Eosinophil Isolation Kit

CD16 MicroBeads (depletion)

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M-MDSC: monocytic myeloid derived suppressor cellG-MDSC: granulocytic myeloid derived suppressor cellIC: immunocomplexes

Human myeloid cells from peripheral blood

Peripheral bloodWhole blood Buff y coat LRSC

Density gradient

PBMCs