Figure S1: Mathematical model of CD14 signaling pathway

Figure S2: Mathematical model of PI3K signaling pathway

Figure S3: Mathematical model of TNF signaling pathway

Figure S4: Mathematical model of EGF signaling pathway

Figure S5: Mathematical model of MAPK signaling networkReaction Species Compartment

Figure S6: Simulation graphs of (A) CD14 (B) TLR4

(A) (B)

Figure S7: Simulation graphs of (A) cytoplasmic NF-kappa B (B) EGF (C) TNF

(A) (B) (C)

Figure S8: Simulation graph of entire MAPK network

(A) (B) (C)

Figure S9: Simulation graphs of TRAF-6 crosstalk point in (A) TNF (B) CD14 (C) MAPK network

(A) (B)

(C) (D)Figure S10: Simulation graphs of MKK3/6 crosstalk point in (A) TNF (B) CD14 (C) EGF (D) MAPK network

(A) (B)

(C) (D)Figure S11: Simulation graphs of MKK4/7 crosstalk point in (A) TNF (B) CD14 (C) EGF (D) MAPK network

(A) (B) (C)

Figure S12: Simulation graphs of PIP2 crosstalk point in (A) EGF (B) PI3K (C) MAPK network

Figure S13: Sensitivity plot of CD14

Figure S14: Sensitivity plot of TLR4

Figure S15: Sensitivity plot of IRAK4/1

Figure S16: Sensitivity plot of EGF

Figure S17: Sensitivity plot of TNF

Figure S18: Sensitivity plot of TRAF-6

Figure S19: Sensitivity plot of cytoplasmic NF-kappa B

Pathways Mean path length Diameter Average

paths

EGF 7 13 26

TNF 5 9 22.6667

CD14 5 9 14.333

PI3K 7 13 10.923

Table S1: Mean path length, diameter and average paths for individual pathways

Clustering coefficient 0.017

Diameter 12

Radius 1

Average paths 10.31

Mean path length 4.902

Number of nodes 72

Number of edges 88

Isolated nodes 0

Self loops 0

Table S2: Statistics of the MAPK network

Reactions Flux

cytoplasm.CD14 → cytoplasm.TLR4 0.485643

cytoplasm.TLR4 → cytoplasm.MyD88 0.211183

cytoplasm.[IRAK4/1] → cytoplasm.[TRAF-6] 0.201431

cytoplasm.TNF → cytoplasm.TNFR 0.369697

cytoplasm.[TRAF-6] → cytoplasm.ECSIT 0.233653

cytoplasm.TAK1 → cytoplasm.[MKK4/7] 0.294193

cytoplasm.ASK1 → cytoplasm.[MKK3/6] 0.265605

cytoplasm.[IkB/NF-kappa B] → cytoplasm.[NF-kappa B] 0.337536

cytoplasm.EGF → cytoplasm.EGF_EGFR 0.491873

cytoplasm.EGF_EGFR → cytoplasm.EGF_EGFR_EGF_EGFR 0.229942

cytoplasm.MEK → cytoplasm.MEKp 0.223247

nucleus.[ERK1/2] → nucleus.Elk 0.216036

Table S3: Reactions with high flux values in the network

Reactions Sensitivity

cytoplasm.CD14 → cytoplasm.TLR4 0.499

cytoplasm.TLR4 → cytoplasm.MyD88 0.581

cytoplasm.[IRAK4/1] → cytoplasm.[TRAF-6] 0.399

cytoplasm.TNF → cytoplasm.TNFR 0.524

cytoplasm.[TRAF-6] → cytoplasm.ECSIT 0.614

cytoplasm.TAK1 → cytoplasm.[MKK4/7] 0.487

cytoplasm.ASK1 → cytoplasm.[MKK3/6] 0.796

cytoplasm.[IkB/NF-kappa B] → cytoplasm.[NF-kappa B] 0.714

cytoplasm.[NF-kappa B] → nucleus.[NF-kappa B] 0.593

cytoplasm.EGF → cytoplasm.EGF_EGFR 0.601

cytoplasm.EGF_EGFR → cytoplasm.EGF_EGFR_EGF_EGFR 0.757

cytoplasm.MEK → cytoplasm.MEKp 0.349

nucleus.[ERK1/2] → nucleus.Elk 0.593

Table S4: Reactions having high sensitivity coefficient (Wi,j) valuesin the network