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StraightFrom® Whole Blood CD14 MicroBeads

StraightFrom® Buffy Coat CD14 MicroBead Kit

StraightFrom® LRSC CD14 MicroBeads

CD16 MicroBeads

Legend

separation differentiation (cultivation)

MACS® Cell Separation products (RUO) MACS Cell Culture and Stimulation products (RUO) MACS Flow Cytometry products (RUO)

DC: dendritic cellcDC: conventional or myeloid DCpDC: plasmacytoid DCMo-DC: monocyte-derived DCPBMC: peripheral blood mononuclear cell

− no expressionlow low expressionint intermediate expression + expression

high high expression+/− expression dependent

on subset

StraightFrom Whole Blood CD15 MicroBeadsStraightFrom Whole Blood CD66b MicroBeads

CD141 (BDCA-3) MicroBead Kit

Myeloid DC Isolation Kit

CD11b MicroBeads

M-CSF/IL-34 + RANKL GM-CSF/IFN-γ + LPS M-CSF

IL-4/IL-13 IC + TLR-Ligands IL-10/TGF-β

IL-4/GM-CSF

CD14 MicroBeadsCD14 MicroBeads UltraPure

Classical Monocyte Isolation Kit

Pan Monocyte Isolation Kit

CD16+ MonocyteIsolation Kit

MACSxpress® NeutrophilIsolation Kit

MACSxpress Eosinophil Isolation Kit

CD1c (BDCA-1) DC Isolation Kit

Myeloid DC Isolation Kit

Diamond Plasmacytoid DC Isolation Kit IIPlasmacytoid DC Isolation Kit IICD304 (BDCA-4/Neuropilin-1)MicroBead Kit

Blood DC Isolation Kit II

Pan-DC Enrichment Kit

Slan (M-DC8)Monocyte Isolation Kit

CD15 MicroBeads

HLA-DR+

MicroBeads +CD14 MicroBeads

Basophil Isolation Kit

Diamond BasophilIsolation Kit

CD123 MicroBeads

Eosinophils and Neutrophils

Neutrophils Eosinophils

CD15+

CD16+

CD66b+

CD177+/−

CD45+

CD14+/−

CD15+/−

HLA-DR+

CD3+/−

CD19+/−

CD56+/−

CD15+

CD66b+

CD11b+

CD15+

CD16+

CD66b+

CD177+/−

CD181 (CXCR1)+

CD182 (CXCR2)+

CD15+

CD16−

Siglec-8+

CD66b+

CD193 (CCR3)+

BasophilsG-MDSCspDCs Pan DCs Slan (M-DC8) DCsCD141+ cDCs

HLA-DR+

CD141 (BDCA-3)+

CD370 (CLEC9A)+

XCR1+

CD123 (IL-3R)−

CD11c+

CD11blow

HLA-DR+

CD303 (BDCA-2)+

CD304 (BDCA-4)+

CD123 (IL-3R)high

CD85g (ILT7)+

CD11clow

CD11c+/−

CD14+/−

CD16+/−

CD33+

CD1c+ cDCs

HLA-DR+

CD172a (SIRPα)+

CD1c (BDCA-1)+

CD11b+

CD123 (IL-3R)int

CD11chigh

CD15+

CD16−

Siglec-8+

CD66b+

CD193 (CCR3)+

EosinophilsNeutrophils

Pan monocytes Non-classicalmonocytes

CD14high

CD16−

CD15−

HLA-DR+

CD14+/int

CD16+/−

CD15−

HLA-DR+

CD14int

CD16high

CD15−

HLA-DR+

Classicalmonocytes

HLA-DR+

CD3−

CD14−

CD19−

CD56−

HLA-DR+

Slan (M-DC8)+

CD11c+

CD14int

CD16high

CD11bdim

FcεRI+

CX3CR1high

CD115+

CD15+

CD66b+

LOX-1+

CD11b+

HLA-DRlow

CD14+

CD33+

CD11b+

CD15−

CD203c+

CD123+

aIgE+

M-MDSCs Myeloid cells (mix)

CD14−

CD11b+

CD209 (DC-SIGN)high

HLA-DR+

HLA-DR+

CD80+

CD86+

Cathepsin K+ MMP-9+

Osteoclasts Mo-macrophages(M1-like)

HLA-DR+

CD86+

iNOS+

Mo-macrophages(M2b-like)

CD163+

CD206+

Arg1+

Mo-macrophages (M2a-like)

CD206+

SLAM+

Arg1+

Mo-macrophages(M2c-like)

Mo-DCs

Eosinophil Isolation Kit

CD16 MicroBeads (depletion)

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This publication is provided for information purposes only, and the contents hereof are subject to change without notice. While Miltenyi Biotec has taken great care and attention to ensure the quality of the information contained herein at the time of publication, no warranties are provided that the content of this publication is accurate, complete, reliable, up to date, and error-free. Miltenyi Biotec assumes no responsibility or liability arising out of the application or use of any information described herein except as expressly confirmed in writing by authorized representative of Miltenyi Biotec. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, the MACS logo, MACSxpress, and StraightFrom are registered trademarks or trademarks of Miltenyi Biotec GmbH and/or its affiliates in various countries worldwide. Copyright © 2017 Miltenyi Biotec GmbH and/or its affiliates. All rights reserved.

M-MDSC: monocytic myeloid derived suppressor cellG-MDSC: granulocytic myeloid derived suppressor cellIC: immunocomplexes

Human myeloid cells from peripheral blood

Peripheral bloodWhole blood Buff y coat LRSC

Density gradient

PBMCs