lecture 6 cell integrity - by vladimir sytnyk
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Lecture 6 Cell Integrity - By Vladimir SytnykTRANSCRIPT
7/18/2019 Lecture 6 Cell Integrity - By Vladimir Sytnyk
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1/5/201
5
Maintaining
Cell
Integri
ty
Dr. Vladimir
Sytnyk
Senior
lecturer,
Head,
Molecular
Neuroiolog
y !aoratory
Sc"ool o#
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y and
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7/18/2019 Lecture 6 Cell Integrity - By Vladimir Sytnyk
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cyto%keleton
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reticulum
mitoc"ondria
Nucleu%
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aaratu%
rganelle%,
rotein%,
Nutrient%,
MEMBRANE
+nergy 3,
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7/18/2019 Lecture 6 Cell Integrity - By Vladimir Sytnyk
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Ha(e to %tay
in%ide o# t"e
cell
7/18/2019 Lecture 6 Cell Integrity - By Vladimir Sytnyk
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(er(i
e* o#
oday’%
!ecture
Membrane
s
Compositio
n
7/18/2019 Lecture 6 Cell Integrity - By Vladimir Sytnyk
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Structure
and
Properties
Fluid Mosaic
Model
Fluidity and
Sidedness
Role of Lipids
in Maintaining
Integrity
Role ofProteins in
Maintaining
Integrity
Membrane
permeability
Memr
ane
Model%
phospholipid
bilayer with
7/18/2019 Lecture 6 Cell Integrity - By Vladimir Sytnyk
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hydrophobic
zone
different
organisation
of membrane
proteins
Fluid Mosaic
Model
Davson-
Danielli
Model
(Singer &
Nicholson, 1972)
(1935)
7/18/2019 Lecture 6 Cell Integrity - By Vladimir Sytnyk
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Sidedne%%The plasma
membrane
has distinct
cytoplasmic
and
extracellular
faces.
The
extracellular
face is
topologically
equivalent to
the inside
face of the
ER, Golgi,
lysosome and
vesicle
membranes.
Proteins and
lipids doNOT
easily “flip”
from one side
of the
7/18/2019 Lecture 6 Cell Integrity - By Vladimir Sytnyk
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membrane to
the other.
1
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1/5/2015
Memer% o# t"e memrane
Memer% o# t"e memrane
1. Lipids
Hydrophilic
2. Proteins
•
Peripheral
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Hydrophobic
•
Integral
Transmembrane:
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Proteins that span
Cholesterol
the membrane but
(0 - 25% of total lipid)
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have different
domains on each
side of the
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membrane
Phospholipids
7/18/2019 Lecture 6 Cell Integrity - By Vladimir Sytnyk
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luid Mo%aic Model
Fluidity refers to the rapid movement of lipids
and proteins laterally, in the plane of the
membrane
hybrid cell
proteins are mixed
mouse
cell
human
after 1 hour
cell
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Rapid movement of lipids and proteins laterally, in the plane of
the membrane can also be visualised using microscopy
luore%cent roe
rotein
Source6 Sytnyk7% Molecular Neuroiology !a
Rapid movement of lipids and proteins laterally, in the plane of
the membrane can also be visualised usingFRAP -
fluorescence recovery after photo- bleaching
Integral rotein tagged *it"
- 8 green 9uore%cent rotein
of lipids and laterally, in the
using
-
2
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1/5/201
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7/18/2019 Lecture 6 Cell Integrity - By Vladimir Sytnyk
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