miltenyibiotec.com
StraightFrom® Whole Blood CD14 MicroBeads
StraightFrom® Buffy Coat CD14 MicroBead Kit
StraightFrom® LRSC CD14 MicroBeads
CD16 MicroBeads
Legend
separation differentiation (cultivation)
MACS® Cell Separation products (RUO) MACS Cell Culture and Stimulation products (RUO) MACS Flow Cytometry products (RUO)
DC: dendritic cellcDC: conventional or myeloid DCpDC: plasmacytoid DCMo-DC: monocyte-derived DCPBMC: peripheral blood mononuclear cell
− no expressionlow low expressionint intermediate expression + expression
high high expression+/− expression dependent
on subset
StraightFrom Whole Blood CD15 MicroBeadsStraightFrom Whole Blood CD66b MicroBeads
CD141 (BDCA-3) MicroBead Kit
Myeloid DC Isolation Kit
CD11b MicroBeads
M-CSF/IL-34 + RANKL GM-CSF/IFN-γ + LPS M-CSF
IL-4/IL-13 IC + TLR-Ligands IL-10/TGF-β
IL-4/GM-CSF
CD14 MicroBeadsCD14 MicroBeads UltraPure
Classical Monocyte Isolation Kit
Pan Monocyte Isolation Kit
CD16+ MonocyteIsolation Kit
MACSxpress® NeutrophilIsolation Kit
MACSxpress Eosinophil Isolation Kit
CD1c (BDCA-1) DC Isolation Kit
Myeloid DC Isolation Kit
Diamond Plasmacytoid DC Isolation Kit IIPlasmacytoid DC Isolation Kit IICD304 (BDCA-4/Neuropilin-1)MicroBead Kit
Blood DC Isolation Kit II
Pan-DC Enrichment Kit
Slan (M-DC8)Monocyte Isolation Kit
CD15 MicroBeads
HLA-DR+
MicroBeads +CD14 MicroBeads
Basophil Isolation Kit
Diamond BasophilIsolation Kit
CD123 MicroBeads
Eosinophils and Neutrophils
Neutrophils Eosinophils
CD15+
CD16+
CD66b+
CD177+/−
CD45+
CD14+/−
CD15+/−
HLA-DR+
CD3+/−
CD19+/−
CD56+/−
CD15+
CD66b+
CD11b+
CD15+
CD16+
CD66b+
CD177+/−
CD181 (CXCR1)+
CD182 (CXCR2)+
CD15+
CD16−
Siglec-8+
CD66b+
CD193 (CCR3)+
BasophilsG-MDSCspDCs Pan DCs Slan (M-DC8) DCsCD141+ cDCs
HLA-DR+
CD141 (BDCA-3)+
CD370 (CLEC9A)+
XCR1+
CD123 (IL-3R)−
CD11c+
CD11blow
HLA-DR+
CD303 (BDCA-2)+
CD304 (BDCA-4)+
CD123 (IL-3R)high
CD85g (ILT7)+
CD11clow
CD11c+/−
CD14+/−
CD16+/−
CD33+
CD1c+ cDCs
HLA-DR+
CD172a (SIRPα)+
CD1c (BDCA-1)+
CD11b+
CD123 (IL-3R)int
CD11chigh
CD15+
CD16−
Siglec-8+
CD66b+
CD193 (CCR3)+
EosinophilsNeutrophils
Pan monocytes Non-classicalmonocytes
CD14high
CD16−
CD15−
HLA-DR+
CD14+/int
CD16+/−
CD15−
HLA-DR+
CD14int
CD16high
CD15−
HLA-DR+
Classicalmonocytes
HLA-DR+
CD3−
CD14−
CD19−
CD56−
HLA-DR+
Slan (M-DC8)+
CD11c+
CD14int
CD16high
CD11bdim
FcεRI+
CX3CR1high
CD115+
CD15+
CD66b+
LOX-1+
CD11b+
HLA-DRlow
CD14+
CD33+
CD11b+
CD15−
CD203c+
CD123+
aIgE+
M-MDSCs Myeloid cells (mix)
CD14−
CD11b+
CD209 (DC-SIGN)high
HLA-DR+
HLA-DR+
CD80+
CD86+
Cathepsin K+ MMP-9+
Osteoclasts Mo-macrophages(M1-like)
HLA-DR+
CD86+
iNOS+
Mo-macrophages(M2b-like)
CD163+
CD206+
Arg1+
Mo-macrophages (M2a-like)
CD206+
SLAM+
Arg1+
Mo-macrophages(M2c-like)
Mo-DCs
Eosinophil Isolation Kit
CD16 MicroBeads (depletion)
Miltenyi Biotec GmbH | Friedrich-Ebert-Straße 68 | 51429 Bergisch Gladbach | Germany | Phone +49 2204 8306-0 | Fax +49 2204 [email protected] | www.miltenyibiotec.com
Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.
130-
118-
514
This publication is provided for information purposes only, and the contents hereof are subject to change without notice. While Miltenyi Biotec has taken great care and attention to ensure the quality of the information contained herein at the time of publication, no warranties are provided that the content of this publication is accurate, complete, reliable, up to date, and error-free. Miltenyi Biotec assumes no responsibility or liability arising out of the application or use of any information described herein except as expressly confirmed in writing by authorized representative of Miltenyi Biotec. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, the MACS logo, MACSxpress, and StraightFrom are registered trademarks or trademarks of Miltenyi Biotec GmbH and/or its affiliates in various countries worldwide. Copyright © 2017 Miltenyi Biotec GmbH and/or its affiliates. All rights reserved.
M-MDSC: monocytic myeloid derived suppressor cellG-MDSC: granulocytic myeloid derived suppressor cellIC: immunocomplexes
Human myeloid cells from peripheral blood
Peripheral bloodWhole blood Buff y coat LRSC
Density gradient
PBMCs