cell-scaffold interaction
DESCRIPTION
Manifestation of Novel Social Challenges of the European Union in the Teaching Material of Medical Biotechnology Master’s P rogrammes at the University of Pécs and at the University of Debrecen Identification number : TÁMOP-4.1.2-08/1/A-2009-0011. - PowerPoint PPT PresentationTRANSCRIPT
Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat the University of Pécs and at the University of DebrecenIdentification number: TÁMOP-4.1.2-08/1/A-2009-0011
CELL-SCAFFOLD INTERACTION
Dr. Judit PongráczThree dimensional tissue cultures and tissue engineering – Lecture 11
Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat the University of Pécs and at the University of DebrecenIdentification number: TÁMOP-4.1.2-08/1/A-2009-0011
TÁMOP-4.1.2-08/1/A-2009-0011
Scaffolds I• Pre-made network of biomaterial• One cell type seeded on one scaffold• Several cell types seeded on a scaffold
simultaneously or sequentially
TÁMOP-4.1.2-08/1/A-2009-0011
Scaffolds IICO2Fused beeds Fused cube porogen Cube porogenFused irregularFleece
TÁMOP-4.1.2-08/1/A-2009-0011
Cell line cells nestling on a scaffold
A549(lung adenocarcinoma cell line)
TÁMOP-4.1.2-08/1/A-2009-0011
Matrigel®Matrigel:• is the trade name for a gelatinous protein
mixture secreted by mouse sarcoma cells• This mixture resembles the complex
extracellular environment found in many tissues
• Matrigel is marketed by BD Biosciences
TÁMOP-4.1.2-08/1/A-2009-0011
Primary SAEC (Small Airway Epithelial Cells) in matrigel
BIOFACTORS
Dr. Judit PongráczThree dimensional tissue cultures and tissue engineering – Lecture 12
Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat the University of Pécs and at the University of DebrecenIdentification number: TÁMOP-4.1.2-08/1/A-2009-0011
TÁMOP-4.1.2-08/1/A-2009-0011
Biofactors
PI3K
Wnt
GSK3
ATF2
b-TrCP
Wnt
TCF
PLCg
TAK
SMADElk1
ATF2c-junc-fos
Hey
HES
Frizzled
Disheveled
FGF
FGFreceptor
BMP
TGF-b
BMP andTGF-b receptor
Notch
Jagged orDelta-likeligands
ICD
Cytosol
Nucleus
Adjacent cell
b-cateninaccumulation
b-catenindegradation
ProliferationDifferentiation
Migration
TÁMOP-4.1.2-08/1/A-2009-0011
Main growth factors (GFs) used in tissue engineeringVEGF – vascular endothelial growth factorKGF – keratinocyte growth factorTGF-b – transfroming growth factor betaBMP – bone morphogenic proteinFGF – fibroblast growth factorPDGF – platelet-derived growth factor
TÁMOP-4.1.2-08/1/A-2009-0011
VEGF• Primarily in endothelial cells• Dimeric glycoprotein• Four splice variants• Receptors: VEGFR-1 and VEGFR-2
TÁMOP-4.1.2-08/1/A-2009-0011
TGF-b• Superfamily member• MW 21 kDa• Synthesized by platelets and macrophages• Induces differentiation in multiple cell types
of various tissues
TÁMOP-4.1.2-08/1/A-2009-0011
BMP• Osteoinductive peptide family• Belongs to the TGF-b superfamily• MW 25-30 kDa
TÁMOP-4.1.2-08/1/A-2009-0011
FGF• 23 members of the family• Multiple functions on multiple cell types• Human FGF-2 18 kDa non-glycosylated
polypeptide binds to heparin and heparan sulfate
TÁMOP-4.1.2-08/1/A-2009-0011
PDGF• Exists as a dimer • Two chains MW 14-17 kDa each• Synthesized by platelets, macrophages,
endothelium, connective tissue• Effects: Mitogenesis, angiogenesis,
macrophage activation
TÁMOP-4.1.2-08/1/A-2009-0011
Delivery of growth factors• Injected• Diffused from scaffolds• Released from gels• Genetically engineered into cells