Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat the University of Pécs and at the University of DebrecenIdentification number: TÁMOP-4.1.2-08/1/A-2009-0011

CELL-SCAFFOLD INTERACTION

Dr. Judit PongráczThree dimensional tissue cultures and tissue engineering – Lecture 11

Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat the University of Pécs and at the University of DebrecenIdentification number: TÁMOP-4.1.2-08/1/A-2009-0011

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Scaffolds I• Pre-made network of biomaterial• One cell type seeded on one scaffold• Several cell types seeded on a scaffold

simultaneously or sequentially

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Scaffolds IICO2Fused beeds Fused cube porogen Cube porogenFused irregularFleece

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Cell line cells nestling on a scaffold

A549(lung adenocarcinoma cell line)

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Matrigel®Matrigel:• is the trade name for a gelatinous protein

mixture secreted by mouse sarcoma cells• This mixture resembles the complex

extracellular environment found in many tissues

• Matrigel is marketed by BD Biosciences

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Primary SAEC (Small Airway Epithelial Cells) in matrigel

BIOFACTORS

Dr. Judit PongráczThree dimensional tissue cultures and tissue engineering – Lecture 12

Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat the University of Pécs and at the University of DebrecenIdentification number: TÁMOP-4.1.2-08/1/A-2009-0011

TÁMOP-4.1.2-08/1/A-2009-0011

Biofactors

PI3K

Wnt

GSK3

ATF2

b-TrCP

Wnt

TCF

PLCg

TAK

SMADElk1

ATF2c-junc-fos

Hey

HES

Frizzled

Disheveled

FGF

FGFreceptor

BMP

TGF-b

BMP andTGF-b receptor

Notch

Jagged orDelta-likeligands

ICD

Cytosol

Nucleus

Adjacent cell

b-cateninaccumulation

b-catenindegradation

ProliferationDifferentiation

Migration

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Main growth factors (GFs) used in tissue engineeringVEGF – vascular endothelial growth factorKGF – keratinocyte growth factorTGF-b – transfroming growth factor betaBMP – bone morphogenic proteinFGF – fibroblast growth factorPDGF – platelet-derived growth factor

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VEGF• Primarily in endothelial cells• Dimeric glycoprotein• Four splice variants• Receptors: VEGFR-1 and VEGFR-2

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TGF-b• Superfamily member• MW 21 kDa• Synthesized by platelets and macrophages• Induces differentiation in multiple cell types

of various tissues

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BMP• Osteoinductive peptide family• Belongs to the TGF-b superfamily• MW 25-30 kDa

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FGF• 23 members of the family• Multiple functions on multiple cell types• Human FGF-2 18 kDa non-glycosylated

polypeptide binds to heparin and heparan sulfate

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PDGF• Exists as a dimer • Two chains MW 14-17 kDa each• Synthesized by platelets, macrophages,

endothelium, connective tissue• Effects: Mitogenesis, angiogenesis,

macrophage activation

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Delivery of growth factors• Injected• Diffused from scaffolds• Released from gels• Genetically engineered into cells