validate spectrophotometric measurements with certified uv/vis reference materials
TRANSCRIPT
Reliable measurements – assured!
Validate spectrophotometric measurements and document their traceability with certified UV/Vis reference materials
• ISO 9001/17025 requires measurement devices to be checked on a regular basis
• GLP (good laboratory practice) governs the responsibility to provide high-quality, accurate data, including instructions for testing devices and reference materials
• Ph. Eur. “European Pharmacopoeia” and USP 857 requires any UV/Vis spectrophotometers in use to be qualified on a regular basis
• USP „U.S. Pharmacopeial Convention“ governs the verification of UV/Vis spectrophotometers
Using certified reference materials to check the measurement device guarantees:
High-quality measurement results
Traceability to international standards
UV/Vis electromagnetic spectrum:
The Hellma Analytics calibration laboratory is accredited for manufacturing and certifying UV/Vis reference materials from 190 to 890 nm
x-ray ultraviolet radiation visible light infrared
Electromagnetic radiation spectrum:
The following UV/Vis spectrophotometer parameters should be checked on a regular basis:
Photometric accuracy (absorbance)
Wavelength accuracy
Stray light level
Spectral resolution
Checking the photometric accuracy (absorbance)
… or with a Neutral Density Glass Filter
Wavelength in nm
I0 I
Light source Detector
using a Potassium Dichromate Liquid Filter
Checking the wavelength accuracy
… or with a Holmium Glass Filter
I0 I
Light source Detector
using a Holmium Liquid Filter
Wavelengths in nm
Checking wavelength and photometric accuracy using a Didymium Glass Filter
Wavelength in nm
Photometric accuracy :
Wavelength accuracy:
Wavelength in nm
I0 I
Light source Detector
Checking the stray light:
with Potassium Chloride (UV1), Sodium Iodide (UV10), Sodium Nitrite (UV11)
Checking spectral resolution using toluene in n-hexane
Wavelength in nm
I0 I
Light source Detector
Traceable
Reliable measurements through the use of certified reference materials.
SRMs
All reference materials certified by Hellma Analytics are traceable to NIST standards
Hellma calibrates its spectrophotometers with standard NIST reference materials on a daily basis
The Hellma Analytics calibration laboratory is:
...certified in accordance with ISO 17025
…Germany’s only accredited DAkkS calibration laboratory for UV/Vis reference materials
DAkkS - Calibration Certificate from Hellma Analytics
Reliability for Certified Reference Materials
In the DAkkS calibration certificate the measured values for each filter are documented.
DAkkS - Calibration Certificate from Hellma Analytics
Reliability for Certified Reference Materials
Glass filter sets for verification of the wavelength accuracy and/or photometric accuracy Quick and easy solutions for calibrating spectrophotometers
Type: 666-S000 Type: 666-S001 Type: 666-S002
Liquid filter sets Quick and easy solutions for calibrating spectrophotometers
Type: 667-UV100
Set for checking stray light in accordance with Ph. Eur.
Type: 667-UV200
Set for checking resolution in accordance with Ph. Eur.
Typ: 667-UV003
Complete set for performing checks in compliance with Ph. Eur.
Reference plate with holmium oxide strips for checking the wavelength accuracy and photometric accuracy of microplate readers The dimensions correspond to a standard microtest plate. Type: 666-R113
Reliable Measurements – Assured!
For further information, please visit: www.hellma-analytics.com/standards or contact us: [email protected]