progesterone’s role in ca2+ influx in spermatozoa

8/13/2019 Progesterones Role in Ca2+ Influx in Spermatozoa

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Progesterones Role in Ca2+Influx in Spermatozoa

1

Amal Katrib

BMP 21


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on0enomi%3ffe%ts of

Progesterone

Mo$ulates t"e a%ti'it& of CatSper'oltage0gate$ permeable Ca2+ %ation

%"annels in sperm tail

Bin$s to eit"er t"e CatSper %"annel or an asso%iate$ subunit

In$u%es influx of Ca2+ into "uman sperm

Ca2+ is essential for

ultimatel& penetration of t"e egg

Capacitation Chemotaxis

Hyperactivation Acrosome Reaction


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Progesterone in Spermatozoa


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Progesterone 6 Ca 2+ Influx

Bip"asi% Signal Components

Rapi$ (ransient

Slo)er Sustaine$


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Ca2+ Inta8e . C"annel (&pe

Ion0C"annel Re%eptor

CatSper Channel


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Pro$u%tion of Ca2+ Signaling in

Spermatozoa

Ca2+ signaling in spermatozoa %an o%%ur $ue to

Progesterone. $ire%t a%ti'ation of CatSper %"annels

NH4Cl. 'ia al8alinit& ,p: ;


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Ca2+ Signaling 0 Progesterone 6

:5Cl


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Cont$

How do we determine whether or not progesterone directly

activates CatSper channels or has impact on pH?

YES/ progesterone is asso%iate$ )it" CatSper %"annels

NO/ progesterone "as no impa%t on p:

What about the joint role of progesterone and NH4Cl in

activating CatSper channels?


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Pat%"0Clamp Re%or$ings

pH !

pH "

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m?)at%

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APBP

1

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Summar&

Progesterone 6 :5Cl in$u%e CatSper %urrent/

stimulating rapi$ Ca2+ influx

("e& a%t s&nergisti%all&

CatSper %"annel/ or asso%iate$ protein/ ser'es as t"e non0

genomi% progesterone re%eptor

Progesterone is essential in fertilit&

Ca %"annel blo%8ers ,C 770>4=9 6 mibefra$il- in"ibit

Ca2+ signals $ue to progesterone 6 al8aline p:


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Clini%al Impli%ations

P"arma%ologi%all& $isrupt fertilization b& in"ibiting CatSper

%"annel )it" ne) t&pe of birt" %ontrol

e) Birt" %ontrol )ill Spe%ifi%all& target progesterones effe%t on sperm

3liminate si$e effe%ts $ue to s&nt"eti% forms of progesterone

Raise a)areness regar$ing infertilit&

asso%iate$ )it" beta0%"annel blo%8ers


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Referen%es

S@ Pubi%o'er 6 C@ Barratt@ Progesterones gate)a& into sperm@ ature

511-@ An$@ (@

Strun8er et al@ ("e CatSper %"annel me$iates progesterone0in$u%e$Ca2+ influx in "uman sperm@ ature 5

progesterone’s role in ca2+ influx in spermatozoa

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