Presentation - DNA Origami Project

Beatrix Fulop, Alice Joffard, Norbert Deak and Ioannis

Panagiotas

20/01/2017

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Introduction

I Goal : Design shapes with DNA origami.

I Tools :

I Cadnano

I Maya

I CanDo

I Outline :

I The principle

I Our design

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The principle

I Step 1 : Build the geometric model by reconstructing the

desired shape with rectangles.

I Step 2 : Add one crossover every k = 16 bases.

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The principle

I Step 3 : Design the scaffold strain and add its corresponding

crossovers.

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The principle

I Step 4 : Design the other portions of strains, the staples,

taking into account the crossovers.

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The principle

I Step 5 : Merge adjacent staples when possible.

I Step 6 : Sequence the scaffold, create the staples as its

complements.

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Our design

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Our design

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Our design

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Our design

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Our design

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Sad smiley

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Mario

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References

I Rothemund, P. W. (2006). Folding DNA to create nanoscale

shapes and patterns. Nature, 440(7082), 297-302.

I Rothemund, P. W. Folding DNA to create nanoscale shapes

and patterns Supplementary Notes 1–11.

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