eat your veggies: anti-cancer crops - minnesota … sustainabl… · · 2018-01-12eat your...

Eat Your Veggies:

Anti-Cancer Crops

Dr. Charlie Rohwer

University of Minnesota Southern Research and Outreach Center

What do WE do at the SROC?

• Feed Vegetables to Humans

– Masonic Cancer Center

• Study Cancer

– Hormel Institute, MCC

Grow vegetables that contain

MORE phytonutrients

Collaborate

Brassicas

Vegetables fight cancer

• Evidence?

0

500

1000

1500

2000

2500

3000

Blu

eberr

ies

Bla

ckberr

ies

Kale

Str

aw

berr

ies

Spin

ach

Ra

spberr

ies

B. spro

uts

Plu

ms

Alfalfa s

pro

uts

Beets

Ora

nges

Red G

rapes

Cherr

ies

Kiw

i

Gra

pefr

uit

OR

AC

Blueberries

• Antioxidants

R. Prior, USDA-ARS

Blueberries

• Antioxidants

– “…[ORAC] values indicating antioxidant capacity

have no relevance to the effects of…polyphenols

on human health”

USDA ARS, ORAC of Selected Foods, Release 2 (2010)

Blueberries

• Antioxidants

– “…[ORAC] values indicating antioxidant capacity

have no relevance to the effects of…polyphenols

on human health”

– “The data of antioxidant capacity of food

generated by in vitro methods cannot be

extrapolated to in vivo effects…”

USDA ARS, ORAC of Selected Foods, Release 2 (2010)

0

500

1000

1500

2000

2500

3000

Blu

eberr

ies

Bla

ckberr

ies

Kale

Str

aw

berr

ies

Spin

ach

Ra

spberr

ies

B. spro

uts

Plu

ms

Alfalfa s

pro

uts

Beets

Ora

nges

Red G

rapes

Cherr

ies

Kiw

i

Gra

pefr

uit

OR

AC

Blueberries

• Antioxidants

R. Prior, USDA-ARS

Goji berries?

http://recipes.howstuffworks.com/

Retreived Feb 8, 2013

http://recipes.howstuffworks.com/

Blueberries

• Antioxidants

• Anti-Aging

Joseph et al., 1999; J. Neurosci (19:8114-8121)

Blueberries

• Antioxidants

• Anti-Aging

Krikorian et al., 2010; J. Agric Food Chem [58(7):3996-4000]

Not significant

Cabbage Family Boasts Nutrients...

Liu et al., Int J. Urology 19(2):134-141

Evidence

• Be especially critical of evidence that agrees

with your opinions

Evidence

• Be especially critical of evidence that agrees

with your opinions

• “On the road from the City of Skepticism, I

had to pass through the Valley of Ambiguity.”

– Adam Smith

Evidence?

• Why do we think vegetables are good for

you?

– Long-Term randomized controlled trials are

expensive & difficult

Evidence?

• Why do we think vegetables are good for

you?

– Long-Term randomized controlled trials are

expensive & difficult

– Separating vegetable intake from healthy

lifestyle choices might be difficult

Confounding Evidence

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

<1 1-2 3-5 6+

Wine

Beer

preference

# of drinks per day

Risk of Death

(compared to

abstainers = 1)

Klatsky et al., 2003, Am. J. Epidemiol.

Controlled for age,

sex, race, BMI,

education, marital

status, smoking,

coronary disease

risk

Lifestyle

• Wine

– Chicken, low-fat

cheese, olives, fruit and

veggies, oil, low-fat milk

• Beer

– Soda, sausage,

margarine & butter,

ketchup, chips, cold

cuts…

Johansen et al., 2006, BMJ

How do you grow anti-cancer foods?

How do you grow anti-cancer foods?

• Grow fruits and vegetables

• “A significant protective effect of fruit and

vegetable consumption was found in 126

of 158 dietary studies”

Block, Patterson, and Subar (1992)

Lowest 25% of

vegetable

consumers

Highest 25% of

vegetable

consumers

Relative Risk

of Cancer

1

2

Vegetable

consumption

Low

High

Vegetables are superfoods

Protective

effects of

increased

vegetable

intake (100 g)

on cancers (case-control

meta-analysis)

Riboli and Norat (2003)


Protective

effects of

increased

vegetable

intake (100 g)

on cancers (cohort meta-

analysis)

Riboli and Norat (2003)

Vegetables are superfoods?

Working Intermission


• Eating this vegetable will prevent cancer


• Eating fruits and vegetables will prevent

cancer


• Studies suggest that people who eat more

vegetables and fruits may have a lower risk

for some types of cancer.

ACS


• “Three grams of soluble fiber from oatmeal

daily, in a diet low in saturated fat and

cholesterol, may reduce the risk of heart

disease. This oatmeal has 2 grams per

serving.”

Phytonutrients

Your

Colors!

Carrots, squash, sweet corn, yellow peppers

(carotenoids and flavonoids)

Blueberries, blackberries, purple cabbage, apples

(anthocyanins and phenolics)

Tomatoes, radishes, red peppers

(lycopene and anthocyanins)

Onions, garlic, shallots

(allicin)

Leafy greens, spinach, kale, brassicas

(lutein, indoles, glucosinolates)

Glucosinolates

Brassicas

Glucosinolates

Glucosinolates

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

Control Carcinogen Carcinogen + PEITC

Cancer

No Cancer

S.S. Hecht et al., Cancer Epidemiol., Biomarkers & Prev. 5:645-652 (1996).

Glucosinolates

• Ingestion

• Absorption

• Metabolism

Glucosinolates

Song and Thornalley, Food Chem.

Toxicol., 2007

In cooking water

In broccoli

Glucosinolates

0

500

1000

1500

2000

2500

3000

3500

4000

Broccoli Brussels Sprouts Cauliflower

Raw

Boiled

Microwaved

Steamed

Data adapted from Pellegrini et al.,

J. Agric. Food Chem., 2010

Aberrant Crypts in Colon

- Precancerous lesions - Correlates with tumor formation

Glucosinolates

Total Aberrant Crypt Foci per cm2

Groups

Ab

err

an

t C

ryp

t F

oci

0

2

4

6

8

10

12

Control HPEITC LPEITC HI3C LI3C

aab ab

bc

c

p=0.02

p=0.001

Andrea Plate and Dan Gallaher

Glucosinolates

Total Aberrant Crypt Per Cm2T

ota

l A

berr

an

t C

ryp

t P

er

Cm

2

0

2

4

6

8

10

12

14

16

ControlBroccoli

WatercressCabbage

a

bb b

p < 0.008

Andrea Plate and Dan Gallaher

Glucosinolates

Fahey J W et al. Cancer Prev Res 2012;5:603-611

Glucosinolates

0

100

200

300

400

500

0 2 4

Glu

cobra

ssic

in (

ug/g

fre

sh w

t.)

Months in Storage (0 C)

‘Grand Slam’

12

18

24

Spacing (in)

Glucosinolates

0

500

1000

1500

2000

0

Glu

co

bra

ssic

in (

ug

/g fre

sh

wt.

)

30

46

61

0

500

1000

1500

2000

4

0

500

1000

1500

2000

2Months in Storage (0 C)

12

18

24

‘Vorox

’

Spacing (in)

[6]-gingerol

[6]-gingerol

angioworld.com

[6]-gingerol

angioworld.com

Kim et al., 2005. Biochem.

Biophys. Res. Comm

[6]-gingerol

Kim et al., 2005. Biochem.

Biophys. Res. Comm

[6]-gingerol

0

2

4

6

8

10

12

178 209

Mg

[6]-

gin

gero

l p

er

gra

m

Days After Planting

Blue

Yellow

0

2

4

6

8

10

12

178 209

Days After Planting

6"

12"

[6]-gingerol

Functional Foods

• “The relationship between fruit and vegetable

intake and cancer prevention has not been

as firmly established and has resulted in

conservative recommendations for

chemoprevention”

Carkeet et al., 2010. Phytochemicals: health promotion and theraputic potential

American Cancer Society

• Does eating vegetables reduce cancer risk?

– “The overall evidence suggests some lowering of

risk for several types of cancer.”

American Cancer Society

• Does eating vegetables reduce cancer risk?

– “The overall evidence suggests some lowering of

risk for several types of cancer.”

• “The best advice is to eat at least 2½ cups of

a variety of colorful vegetables and fruits

each day.”

Charlie

Rohwer

[email protected]

Questions?

Nutraceutical Development

• Production Consistency

• Sensory Acceptance

• Quantification /Certification

• Real /Perceived Value

Carotenoids

0

2

4

6

8

10

12

14

Broccoli Brussels Sprouts Cauliflower

Raw

Boiled

Microwaved

Steamed

Data adapted from Pellegrini et al.,

J. Agric. Food Chem., 2010

Carotenoids

1

10

100

1000

Brussels Sprouts Carrots

Raw

Steamed

Sous Vide 5d

Data adapted from Miglio et al., J.

Agric. Food Chem., 2008

Glucosinolates

Myrosinase

Glucoraphanin

Sulforaphane

(bioactive)

Glucosinolates

Myrosinase

Glucoraphanin

Sulforaphane

(bioactive)

DTC

(excreted)

Glucosinolates

Glucoraphanin Sulforaphane

Myrosinase

INACTIVE ACTIVE

Glucosinolates

Glucoraphanin Sulforaphane

Myrosinase

INACTIVE ACTIVE

Myrosinase

Glucoraphanin

• Amercians don’t eat as much vegetables

as they probably should

Dong and Lin, 2009



0

0.5

1

1.5

2

2.5

3

Dark Green

Orange Starchy Other Total

Recommended Intake

Dong and Lin, 2009


0

0.5

1

1.5

2

2.5

3

Dark Green

Orange Starchy Other Total

Recommended Intake

Average Intake

Dong and Lin, 2009

[6]-gingerol

eat your veggies: anti-cancer crops - minnesota … sustainabl… · · 2018-01-12eat your...

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