delphinium exaltatum (ranunculaceae) of the ......powerpoint presentation author kelsey huisman...
TRANSCRIPT
Conservation genetics of the rare species Delphinium exaltatum (RANUNCULACEAE) of the
Appalachian mountains and the Ozark highlands of Missouri
Kelsey Huisman Northern Michigan University
Dr. David Bogler Missouri Botanical Garden
INTRODUCTION
objective
Examine the genetic variation and differentiation within and among
populations of D. exaltatum from the Appalachian mountains and the Ozark highlands of Missouri to distinguish the effects of geographic isolation.
METHODS
study sites
• Habitat – Dolomite glades with alkaline soils
– An average of 65% shade
– Primarily found on southwest to northwest facing slopes
• Associated plant species – >85% Chinkapin oak (Quercus
muehlenbergii)
– Hairy angelica (Angelica venenosa)
• Encroaching plant species – Cedar (Juniperus virginiana)
– Sugar maple (Acer saccharum)
• Cauline • Petiolate
• Deeply lobed
• Raceme of 8-30 blue to purple flowers
• Individual flowers are radially symmetrical with five sepals
• One of the
sepals creates a spur
• Height is between 4 – 6 feet, and at times taller
life cycle
• April-July: Vegetative • July-September: Flowering • August-October: Fruit • Mid to late November-March: Dormant
extractions
amplification
1st PCR cycle
gel-stab technique
2nd PCR cycle
sequencing
• Yale University – DNA Analysis Facility • Sanger sequencing
Missouri, Howell County sample 11 – large band, forward sequence
RESULTS
Figure 1. Likelihood analysis. Missouri populations are shown in green.
DISCUSSION
ACKNOWLEDGEMENTS
• National Science Foundation
• National Park Service
– Daniel Drees
– Christine Steinwand
• Missouri Botanical Garden
– Dr. David Bogler
– Justin Zweck
– Dr. Rosa Ortiz-Gentry
– Missouri Botanical Garden Staff
• REU Students
Questions?!