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Conservation genetics of the rare species Delphinium exaltatum (RANUNCULACEAE) of the
Appalachian mountains and the Ozark highlands of Missouri
Kelsey Huisman Northern Michigan University
Dr. David Bogler Missouri Botanical Garden
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INTRODUCTION
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objective
Examine the genetic variation and differentiation within and among
populations of D. exaltatum from the Appalachian mountains and the Ozark highlands of Missouri to distinguish the effects of geographic isolation.
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METHODS
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study sites
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• Habitat – Dolomite glades with alkaline soils
– An average of 65% shade
– Primarily found on southwest to northwest facing slopes
• Associated plant species – >85% Chinkapin oak (Quercus
muehlenbergii)
– Hairy angelica (Angelica venenosa)
• Encroaching plant species – Cedar (Juniperus virginiana)
– Sugar maple (Acer saccharum)
• Cauline • Petiolate
• Deeply lobed
• Raceme of 8-30 blue to purple flowers
• Individual flowers are radially symmetrical with five sepals
• One of the
sepals creates a spur
• Height is between 4 – 6 feet, and at times taller
life cycle
• April-July: Vegetative • July-September: Flowering • August-October: Fruit • Mid to late November-March: Dormant
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extractions
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amplification
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1st PCR cycle
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gel-stab technique
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2nd PCR cycle
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sequencing
• Yale University – DNA Analysis Facility • Sanger sequencing
Missouri, Howell County sample 11 – large band, forward sequence
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RESULTS
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Figure 1. Likelihood analysis. Missouri populations are shown in green.
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DISCUSSION
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ACKNOWLEDGEMENTS
• National Science Foundation
• National Park Service
– Daniel Drees
– Christine Steinwand
• Missouri Botanical Garden
– Dr. David Bogler
– Justin Zweck
– Dr. Rosa Ortiz-Gentry
– Missouri Botanical Garden Staff
• REU Students
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Questions?!