betula uber

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JORESSIA A. JAMISON AND MICHAEL H.RENFROE

Department of Biology, JamesMadison University, Harrisonburg,

Virginia 22807

(Received 21 March 1997; accepted23 January 1998; editor T. A. Thorpe)

MICROPROPAGATIONOF BETULA UBER

MikropropagacijaBetule uber



Virginijskaokroglolistna

breza



GOJIE

Package SizePrice

($USD)

1L $1.25

10L $8.2550L $27.00



+ hormoni (citikinini)

+

Autoklaviranje121°C1,05kg/cm215 min

8 g/L DifcoBocto agar

1M 5M 10 M

- 6 benzilamino adenin (BA) ali

- 2-izopentenil adenin (2iP) ali

- kinetn



IZSEEK 4 - 6 SADIK(14,5 ± 64 cm)

Pomlad, poletje,

Mlado drevo,dobro razvejano

Sterilizacija

(15% Clorox 15 min)

7× destilirana voda

Nodije obreemo

-pokodovano tkivo

Kozarki

(babyfood jars)

Petrijevka

SEGMETI z

3 ± 4 nodiji

Spiranje izseka

Tween 20 (5 min)



RASTN E KOMO R E

Dnevni cikel

28°C

15 ur (6:00-21:00)

Noni cikel

18°C

9h (21:00-6:00)



BUD BREAK



FIG. 1. Comparison of bud break of Betula uber

nodal explants over timestimulated by 2-iP (A), kinetin (B), and BA ((2) atdifferent concentrations.Each point represents the results from 20 cultureswith one explant per culture.



FIG. 2. Comparison of mean shoot elongationof Betula uber nodal explantsat 4 wk stimulated by BA, 2-iP, and kinetin atdifferent concentrations.Each point represents the results from 20cultures with one explant per culture.

Basalcallusing ±bazalni kalus34% najve

Vitrifikacije NI

Temno zelena rastlina



FIG. 3. Comparison of Betula uber bud break over time stimulated by BAat different concentrations, or BA in combination with GA 3. Each point

representsthe results from 20 cultures with one explant per culture.

FIG. 4. Comparison of mean shoot elongationof Betula uber nodal explantsover time stimulated by BA at differentconcentrations, or BA in combinationwith GA 3. Each point represents the resultsfrom 20 cultures withone explant per culture.

Preverjanje konc. Okrog 10M BA (7,5 M 10 M 12,5 M)+ 2,9 M GA

WPM + BA so neznatnomanj brstele (4 tedni)

WPM + BA in GA dobro

brstenje

1,9 cm

0,325cm

WPM + BA + GA

- bazalni kalus (44%)

- stekleni listi

WPM + BA

- ni steklenih listov



POGANJEK 1,5 CM

P R ESA J AN JE I N KO R EN I N JEN JE

ZEMLJA

Promix/perlite

WPM + BA + GAslabo preneslepresajanje

WPM + BA

Dobro preivelepresajanje

77,8%

Najbolje ukoreninjenje WPM + 12,5 M BAGojie brez hormonov neuspenokoreninjenje



RAZ I S K AV E / ANAL I Z E




Polisadnimezofil(parenhim)

Debelinapovrhnjice intrihomi

Gostota in velikostlistnih re

SEM in vrstini elektronski mikroskop




- po 1 tednu v in vitro

- po fazi prenosa

- v zemlji ( 2. teden)

- po aklimatizaciji



FENOTIPSKESPREMEMBE



S K L E P I

Mona mikropropagacija Butule uber Gojie WPM z BA in GA nam daje najhitreje brstenje

Betule uber ± giberelinsko odzivna vrsta Aklimatizirane rastline imajo debelejo kutikolo Na podlagi re smo ugotovili FENOTIPSKI ODZIV kiomogoi preivetje rastlin glede na razmere

Te tehnike se lahko izkoristi za uporabo propagacije ostalihlistavcev ki so e ogroeni poleg Betule uber

betula uber

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