baxx report issue 1

8/8/2019 Baxx Report Issue 1

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1.0 This report returns the results of initial tests conducted with a device called theBaxx system. The Baxx device is intended to operate in a manner which constantly

delivers a Hydroxyl cloud capable of achieving both sanitizing effects and odour

control. The device assessed in this study is intended for treating spaces and surfaces of up to 250 m3.

The objectives of this trial were as follows ;

a) To demonstrate the efficiency of the device in reducing microbial contamination

on solid surfaces during continuous usage.

b) To demonstrate the efficiency of the device in the reduction of atmospheric levels

of microorganisms during continuous usage.

c) To demonstrate the impact on the rancidity characteristics of whole birds (p+1 )under continuous exposure at 5’c

d) To demonstrate any reduction in microbial contamination of whole birds (p+1 )

under continuous exposure at 5’c

2.0 Method Outline

2.1 Assessment of the reduction of microbial contamination on solid surfaces ;

This experiment was conducted in two 250 m3 laboratory spaces. During the trial area A

was continuously treated with the Baxx device while area B was not treated The

microbial challenge consisted of plastic templates ( 700 cm2) which were divided intoa series 25 cm2 sectors and which were seeded with individual microbial cultures

dispersed in Brain heart infusion. The surfaces were allowed to dry prior to the trial. Six

templates for each organism were distributed randomly in each of the test environments

The following organisms were employed ;

Salmonella seftenbergStaphylococcus aureus

Pseudomonas aeruginosa

Aspergillus niger Listeria monocytogenes

Escherichia coli



On each day of the trial random plastic pieces were selected in each environment for each

organism and the viable number of organisms for duplicate 25 cm2 was determined after recovery by swabbing followed by serial dilution and analysis by spiral platting on

appropriate microbiological media.

In this manner any effect antimicrobial produced by the Baxx treatment could be

assessed by comparison the result obtained in an untreated environment

2.2 Assessment of reduction in Atmospheric contamination

This experiment was conducted in a waste room situated in a microbiological

laboratory. This environment is intended to store bags of Class I and Class II waste

intended for sterilisation by autoclaving. Due to the nature of the work carried out in thisenvironment there is possibility of atmospheric contamination by microorganisms.

The quality of the atmosphere was assessed by volumetric sampling of a 50 Litre

volume four times per day ( 0800, 1200, 1600 & 2000 hrs) employing an impaction air

sampler. The quantities determined were aerobic plate count , Yeast count and the Mould

count. The results of these determinations are expressed as mean count per Litre of atmosphere per day over the trial period.

Control sampling was conducted over a seven day period prior to Baxx treatment andadditionally over a further seven day period with the Baxx device running continuously.

The volume of the room was 70 m3.

By comparing mean counts for the measured parameters throughout the untreated andtreated period an estimate of atmospheric microbial reduction has been achieved.

2.3 Assessment on the impact on rancidity and microbial reduction characteristics of

Free range whole birds (p+1 ) under continuous exposure to Baxx treatment at 5’c .

This series of experiments was conducted in two chiller units (134 m3). 12 birds werelocated in each chiller. Chiller A was untreated while chiller B was exposed to continuos

dosing by operation of the Baxx device over a 12 hour period.

During the trial period duplicate samples of breast skin were removed from each

population on an hourly basis. The fat from these samples was extracted in the dark with

cold Chloroform and subsequently the F.F.A. and P.V. values were determined.

Sub samples of skin were examined to determine the levels of Pseudomonas sps, Yeastsand Campylobacter sps.



3.0 Results section

Results for the reduction of organisms on plastic surfaces due Baxx treatment

Table 1 : Survival characteristics of microbial cultures on plastic surfaces situated in

a 250 M3 space during continuous treatment with Baxx device.

DaysOrganism 1 2 3 4 5 6 7 Log reductionSalmonella seftenberg 1.30E+07 8.20E+06 1.80E+05 7.20E+04 4.90E+03 6.30E+01 0.00E+00 >7Staphylococcus aureus 2.10E+07 7.10E+06 1.90E+05 6.10E+04 7.20E+03 1.10E+02 0.00E+00 >7Pseudomonas aeruginosa 2.40E+07 8.40E+06 2.40E+05 5.20E+04 4.30E+03 4.00E+01 0.00E+00 >7Aspergillus niger 3.10E+07 7.10E+06 6.20E+05 6.00E+04 9.30E+03 9.00E+02 1.30E+02 >6Listeria monocytogenes 1.20E+07 6.00E+06 6.10E+05 2.30E+04 5.00E+03 3.30E+01 0.00E+00 >7Escherichia coli 1.30E+07 2.10E+06 1.30E+05 9.20E+03 7.00E+02 5.20E+01 0.00E+00 >7

All data cfu/cm2

Figure 1 ; Survival characteristics of microbial cultures on plastic surfaces situated in

a 250 M3 space during continuous treatment with Baxx device.

Effect of Baxx treatment on M icrobial Cultures over 7 days

1.00E+00

1.00E+02

1.00E+04

1.00E+06

1.00E+08

1 2 3 4 5 6 7

Day

L o g c f u

/ c m 2

Salmonella seftenberg

Staphylococcus aureus

Pseudomonas aeruginosa

Aspergillus niger

Listeria monocytogenes

Figure 2 a : Spiral plate recovery of Pseudomonas aeruginosa from a plastic surface

prior to Baxx treatment ( 50ul deposition e3 dilution)



Figure 2 b : Spiral plate recovery of Pseudomonas aeruginosa from a plastic surfaceafter 7 days without Baxx treatment ( 50ul deposition e2 dilution)

Figure 2 c : Spiral plate recovery of Pseudomonas aeruginosa from a plastic surface

after 7 days with Baxx treatment. ( 3 x 0.5 ml linear deposition e1 dilution)



Table 2 : Survival c

Table 2b : Log



Results for the Atmospheric reduction of organisms due to Baxx treatment

Table 3 : Mean waste



Results for Rancidity effects on whole Birds Bird Breast Skin due to Baxx

treatments

Results for reduction of microbial contamination in Whole Birds Bird Breast Skin

due to Baxx treatments

Table 6 : Mean

Table 5 : Rancidity level over 12 hours with and without Baxx treatment at 5 'c

Day

1 2 3 4 5 6 7 8 9 10 11 12 Mean

FFA BAXX ON 0.2 0.1 0.2 0.3 0.2 0.2 0.3 0.4 0.2 0.4 0.5 0.6 0.3

FFA BAXX OFF 0.2 0.2 0.2 0.2 0.3 0.2 0.1 0.3 0.3 0.2 0.2 0.3 0.2

PV BAXX ON 2.0 1.0 2.0 2.0 3.0 1.0 2.0 2.0 3.0 4.0 4.0 5.0 2.6

PV BAXX OFF 2.0 2.0 1.0 2.0 3.0 1.0 1.0 2.0 3.0 2.0 2.0 2.0 1.9

Figure 3

1 2 3 4 5 6 7 8 9 10 11 12

FFA BAXX ON

PV BAXX OFF0.0

1.0

2.0

3.0

4.0

5.0

V a l u e

Hours

Rancidity Characteristics over 12 Hours at 5'c for Whole

Birds with and without Baxx doses

FFA BAXX ON

FFA BAXX OF

PV BAXX ON

PV BAXX OFF



Conclusions

Consensus opinion in the literature supports a theory that Hydroxyl dosing of

microorganisms leads to lethality due to irreversible inactivation of essential enzymes.

During this trial we have shown that over a seven day period the Baxx device during

continuous operation will produce between a Log 4 and Log 5 reduction of common

food spoilage organisms and pathogens on the surface of plastic templates.

Additionally our work indicates that in an area subject to frequent recontamination by

microbiological waste the Baxx device is capable of delivering a constant 2 log

improvement in atmospheric levels of Total viable count and Fungi ( Yeasts andMoulds).

Further by exposing whole birds to dosing with the Baxx device we have been able todemonstrate significant reduction in breast skin contamination by Pseudomonas species

( 2.7 Log ) , Campylobacter species (1.7 Log) and Yeasts ( 1.8 Log)

On the basis of these results there exists a strong case that microbiological quality of food

production environments could be improved by regular prolonged or continuous dosagewith the Baxx unit.

Our results for the impact of prolonged ( 12 hours) Baxx treatment in relation to thedevelopment of rancidity in the breast skin of free range whole birds indicates that after

10 hours of dosing there is a significant upward trend in Peroxide value . ( Treated after

12 hours 5.0 Untreated after 12 hours 2.0) . A similar trend in F.F.A was not observed.

My opinion is that further work across several batches of different types of birds isrequired to establish definitively the impact of long term( greater than 10 hours) Baxx

dosing of primary materials with regard to rancidity and organoleptic effects.

…………………………………………………………..

D.O’Connor M.Sc. B.Sc..Mi. Biol. Ci.Biol M.I.F.S.T.

baxx report issue 1

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