genetic control of temperature preference in the nematode caenorhabditis elegans akiko mohri, elji...
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Genetic Control of TemperaturePreference in the Nematode
Caenorhabditis elegans
Akiko Mohri, Elji Kodama, Koutarou Kimura, Mizuho Koike, Takafumi Mizuno and Ikue Mori
Background
• Worms will migrate to cultivation temperature when on a temperature gradient (H&R, 1975)
• Worm will avoid temperatures paired with starvation
• If E. coli paired with new temperature, take 2-4 hours before worms will migrate to the new temperature
Neural circuit
Mori 1995
25˚C
15˚C
Thermotaxis Phenotypes
Isothermic
ThemophilicCryophilic
Athermotactic
N2aho-2hen-1
No ablation
ttx-3nsc-1
AFD/AIY ablation
lin-11
AIZ ablation
tax-2tax-4sel-12
RIA ablation
25˚C
15˚C
25˚C
15˚C
25˚C
15˚C
Figure 1
Table 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 10
Figure 11
Figure 12
Conclusions
• Respond differently to changes in temperature vs. feeding state
• aho-2 defective thermotactic avoidance
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