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Wnt, Notch & Hedgehog Pathway Antibodies

Wnt Pathway AntibodiesSymbol Catalog # Select Antibodies Reactivity Applications

AKT1 A302-065A AKT1 Antibody Hu WB PLA IHC-P IF

APC A310-318A APC AbVantage™ Pack Hu WB IP IHC-P IF

ARRB2 A311-391A Beta-Arrestin 2 AbVantage

Pack

Hu WB IP

CD44 A303-872A CD44 Antibody Hu WB IP

CREBBP A310-098A CBP AbVantage Pack Hu WB IP IHC-P IF

PLA

CSNK1A1 A301-991A CKI alpha Antibody Hu Ms WB IP PLA

CSNK1D A302-136A CKI delta Antibody Hu WB IP

CSNK1E A302-135A CKI epsilon Antibody Hu WB IP

CSNK2A1 A310-054A CKII Alpha AbVantage Pack Hu WB IP IHC IF

PLA

CTBP1 A300-338A CTBP1 Antibody Hu WB PLA IHC-P IF

CTBP2 A311-148A Ribeye AbVantage Pack Hu Ms WB IP

CTNNB1 A310-710A Beta-catenin AbVantage

Pack

Hu Ms WB IP IHC-P IF

PLA

CUL1 A311-246A Cul1 AbVantage Pack Hu Ms WB IP PLA

DVL2 A311-095A Dishevelled 2 AbVantage

Pack

Hu WB IP

EP300 A310-097A p300 AbVantage Pack Hu WB IP ChIP PLA

IHC IF

FZD6 A311-098A Frizzled-6 AbVantage Pack Hu IP

GSK3B A310-723A GSK3-beta AbVantage Pack Hu Ms WB IP PLA

HDAC1 A310-214A HDAC1 AbVantage Pack Hu Ms WB IP IHC-P IF

PLA

HNF1A A311-457A HNF1-alpha AbVantage Pack Hu WB IP

JUN A311-097A c-Jun AbVantage Pack Hu Ms WB IP PLA

LEF1 A311-286A LEF1 AbVantage Pack Hu WB IP

MAP3K7 A310-678A TAK1 AbVantage Pack Hu WB IP

NFATC2 A303-131A NFAT1 Antibody Hu WB IP

PAFAH1B1 A300-409A LIS-1 Antibody Hu Ms Rt WB

PPP2CB A310-222A PP2A AbVantage Pack Hu Ms WB IP

PPP2R1A A310-312A PPP2R1A AbVantage Pack Hu Ms WB IP

PPP2R5A A300-967A PPP2R5A Antibody Hu Ms WB IP

PPP2R5C A311-402A PPP2R5C AbVantage Pack Hu WB IP

PPP2R5D A310-361A PPP2R5D AbVantage Pack Hu Ms WB IP

PRKCA A310-887A PKC-alpha AbVantage Pack Hu Ms WB IP

PRKCD A310-893A PKC-delta AbVantage Pack Hu WB IP

PRKCH A311-523A PKC-eta AbVantage Pack Hu WB IP

PRKCQ A311-522A PRKCQ AbVantage Pack Hu WB IP

ROR2 A311-525A ROR2 AbVantage Pack Hu WB IP

TAB1 A310-773A TAB1 AbVantage Pack Hu Ms WB IP

TCF3 A300-260A Phospho E2A (T355) Anti-

body

Hu WB

TLE1 A303-545A TLE1 Antibody Hu WB IP

TP53 A310-068A p53 AbVantage Pack Hu WB IP F PLA

Detection of Human p300 by IHC. Sample: FFPE section of hu-man lung carcinoma. Antibody: Rabbit anti-p300 (Cat. No. A300-358A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

Detection of Human AKT1 by IHC. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-AKT1 (Cat. No. IHC-00513) used at a dilution of 1:250. Detection: DAB.

EP300 A300-358A; HDAC1 A300-713A: Zhang LJ et al., Coordinat-ed Regulation of Transcription Factor Bcl11b Activity in Thymo-cytes by the Mitogen-activated Protein Kinase (MAPK) Pathways and Protein Sumoylation. J Biol Chem. 2012 Aug 3;287(32):26971-88.

HDAC1 A300-712A: Yin F et al., LSD1 Regulates Pluripotency of Embryonic Stem/Carcinoma Cells through HDAC1-mediated Deacetylation of Histone H4 at Lysine 16. Mol Cell Biol. 2013 Nov 4.

PPP2R5A A300-967A: Kirchhefer U et al., Protein phosphatase 2A is regulated by PKCa-dependent phosphorylation of its targeting subunit B56a at Ser41. J Biol Chem. 2013 Nov 13.

PPP2R5D A301-098A: Liu L et al., Regulation of c-Myc Protein Abundance by a Protein Phosphatase 2A-Glycogen Synthase Kinase 3b-Negative Feedback Pathway. Genes Cancer. 2012 Jan;3(1):23-36.

MAP3K7 A301-915A: Yu M et al., RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis. Nature. 2012 Jul 26;487(7408):510-3.

Selected Product Citations

Cover: Primary-conjugate Duolink II Proximity Ligation Assay in Hela cells. Goat anti- human RENT1 (A300-038A) and rabbit anti-human MOV10 (A301-571A).

Notch Pathway AntibodiesSymbol Catalog # Select Antibodies Applications

AKT1 A302-065A AKT1 Antibody WB PLA IHC-P IF

APC A310-318A APC AbVantage Pack WB IP IHC-P IF

ARRB1 A303-745A Beta-Arrestin 1 Antibody WB IP

ARRB2 A311-391A b-Arrestin 2 AbVantage WB IP

CCNC A301-989A Cyclin C Antibody WB IP IHC-P IF

CDK8 A310-910A CDK8 AbVantage Pack WB IP IHC-P IF

CIR1 A303-141A CIR1 Antibody WB IP

CREB1 A301-669A CREB Antibody WB IP PLA IHC-P IF

CREBBP A310-098A CBP AbVantage Pack WB IP IHC-P IF PLA

CSNK1A1 A301-991A CKI alpha Antibody WB IP PLA

CSNK2A1 A310-054A CKII-a AbVantage Pack WB IP IHC-P IF PLA

CTBP1 A300-338A CTBP1 Antibody WB PLA IHC-P IF

CTBP2 A311-148A Ribeye AbVantage Pack WB IP

CTNNB1 A310-710A b-catenin AbVantage Pack WB IP IHC-P IF PLA

CUL1 A311-246A Cul1 AbVantage Pack WB IP PLA

DTX3L A310-261A DTX3L/BBAP AbVantage WB IP

DVL2 A311-095A Dishevelled 2 AbVantage WB IP

EP300 A310-097A p300 AbVantage Pack WB IP ChIP PLA

IHC IF

EPS15 A310-617A EPS15 AbVantage Pack WB IP IHC-P IF

FBXW7 A310-612A FBW7 AbVantage Pack IP IHC-P IF

GATA3 A303-502A GATA3 Antibody WB

GSK3B A310-723A GSK3-beta AbVantage WB IP PLA

HDAC1 A310-214A HDAC1 AbVantage Pack WB IP IHC-P IF PLA

HDAC2 A310-211A HDAC2 AbVantage Pack WB IP IHC-P IF ChIP

HDAC3 A300-464A HDAC3 Antibody WB IP IHC-P IF

HDAC4 A311-279A HDAC4 AbVantage Pack WB IP

HDAC5 A311-277A HDAC5 AbVantage Pack WB IP

HDAC6 A310-461A HDAC6 AbVantage Pack WB IP ChIP

HDAC7 A310-480A HDAC7 AbVantage Pack WB IHC-P IF

HIF1A A300-286A HIF1-alpha Antibody WB IP F PLA

HIF1A A303-907A HIF1-alpha Antibody IP PLA

HNF1A A311-457A HNF1-alpha AbVantage WB IP

ITCH A301-992A Itch Antibody WB

JAK2 A302-178A JAK2 Antibody WB IP IHC-P IF

JUN A311-097A c-Jun AbVantage Pack WB IP PLA

KAT2B A301-666A PCAF Antibody WB IP ChIP

KDM1A A310-060A BHC110/LSD1 AbVantage WB IHC-P IF ChIP

LEF1 A311-286A LEF1 AbVantage Pack WB IP

MAML1 A310-198A MAML1 AbVantage Pack WB IP

MAML2 A310-202A MAML2 AbVantage Pack WB IP

MAML3 A310-201A MAML3 AbVantage Pack WB IP

MAP3K7 A310-678A TAK1 AbVantage Pack WB IP

MAPK1 A302-061A ERK2 Antibody WB IHC-P IF PLA

MAPK3 A311-325A ERK1/2 phospho (T202/

Y204 T185/Y187) AbVan-

tage Pack

WB IP

MAPK3 A302-060A ERK1 Antibody WB IP IHC-P IF PLA

Symbol Catalog # Select Antibodies Applications

MARK2 A311-158A MARK2 AbVantage Pack WB IP

MIB2 A301-414A Skeletrophin Antibody WB IP

MOV10 A301-571A MOV10 Antibody WB IP PLA

MOV10 A500-009A MOV10 Monoclonal Ab WB IP IHC

NCOR1 A310-379A NCoR AbVantage Pack WB IP ChIP PLA

NCOR2 A310-380A SMRT AbVantage Pack WB IP PLA

NFKB1 A310-648A NF-kB1 AbVantage Pack WB IP PLA

NOTCH1 A310-672A Notch1 AbVantage Pack WB IP PLA

NOTCH2 A310-736A Notch2 AbVantage Pack WB IP

NUMB A310-609A NUMB AbVantage Pack WB IP

NUMBL A310-611A NUMBL AbVantage Pack IP

PIK3R2 A310-947A PIK3R2 AbVantage Pack WB IP

PPP2R5D A310-361A PPP2R5D AbVantage Pack WB IP

RBBP8 A310-140A CtIP AbVantage Pack WB IP PLA

RBX1 A303-462A ROC1 Antibody WB

RELA A310-650A RelA AbVantage Pack WB IP PLA IHC IF

RELA A302-064A RelA, phospho (S468) Ab WB IHC-P IF PLA

RELA A302-063A RelA, phospho (S529) Ab WB

RELA A302-062A RelA, phospho (S536) Ab WB IHC-P IF

RING1 A303-552A RING1 Antibody WB IP

SAP30 A303-551A SAP30 Antibody WB IP

SET A310-814A SET AbVantage Pack WB IP IHC-P IF

SKI A311-296A c-Ski AbVantage Pack WB IP

SKP2 A310-883A SKP2 AbVantage Pack WB IP

SNW1 A310-244A NCOA62 AbVantage Pack WB IP

SPEN A310-368A SHARP AbVantage Pack WB IP

STAT3 A310-871A STAT3 AbVantage Pack WB IP PLA

TAB1 A310-773A TAB1 AbVantage Pack WB IP

TBL1XR1 A300-408A TBLR1 Antibody WB IP IHC-P IF

TCF3 A300-260A E2A, phospho (T355) Ab WB

TLE1 A303-545A TLE1 Antibody WB IP

TLE2 A303-597A TLE2 Antibody WB IP

TNRC6A A310-844A GW182 AbVantage Pack WB IP

TNRC6C A303-969A TNRC6C Antibody WB IP

UBA52 A300-317A Ubiquitin Antibody WB IHC-P IF

YY1 A311-019A YY1 AbVantage Pack WB IP PLA

Selected Product Citations

HDAC1 A300-712A; HDAC2 A300-704A; HDAC3 A300-464A; HDAC6 A301-341A: Yin F et al., LSD1 Regulates Pluripotency of Embryonic Stem/Carcinoma Cells through HDAC1-mediated Deacetylation of Histone H4 at Lysine 16. Mol Cell Biol. 2013 Nov 4.

HIF1A A300-286A: Finger EC et al., CTGF is a therapeutic target for meta-static melanoma. Oncogene. 2013 Feb 25. doi: 10.1038/onc.2013.47.

RELA, phospho (S468) A302-064A: He Z, et al., NF-kB Activation Coordinat-ed by IKKb and IKKe Enables Latent Infection of Kaposi’s Sarcoma-associated Herpesvirus J Virol. 2013 Oct 23. [Epub ahead of print]

FBXW7 A301-721A, A301-720A: Welcker M et al., Fbw7 dimerization deter-mines the specificity and robustness of substrate degradation. Genes Dev. 2013 Dec 1;27(23):2531-6.

Hedgehog Pathway AntibodiesSymbol Catalog # Selected Antibodies Reactivity Applications

AKT1 A302-065A AKT1 Antibody Hu WB PLA IHC-P IF

ARNTL A302-616A BMAL1 Antibody Hu Ms WB IP

ARRB2 A311-391A b-Arrestin 2 AbVantage Pk Hu WB IP

CCNH A310-593A Cyclin H AbVantage Pack Hu WB IP

CDK1 A311-344A CDK1 AbVantage Pack Hu WB IP PLA

CDK7 A300-405A CDK7 Antibody Hu Ms Rt WB IP PLA

CDK8 A310-910A CDK8 AbVantage Pack Hu WB IP IHC-P IF

CDKN1C A310-851A p57kip2 AbVantage Pack Hu IP

CDKN2A A310-091A p14ARF AbVantage Pack Hu WB IHC-P IF

CREBBP A310-098A CBP AbVantage Pack Hu WB IP IHC IF PLA

CSNK1A1 A301-991A CKI alpha Antibody Hu Ms WB IP PLA

CSNK1D A302-136A CKI delta Antibody Hu WB IP

CSNK1E A302-135A CKI epsilon Antibody Hu WB IP

CTNNB1 A310-710A b-catenin AbVantage Pack Hu Ms WB IP IHC-P IF PLA

CUL1 A311-246A Cul1 AbVantage Pack Hu Ms WB IP PLA

DYRK1A A311-398A DYRK1A AbVantage Pack Hu WB IP

EP300 A310-097A p300 AbVantage Pack Hu WB IP ChIP PLA

IHC IF

EP300 IHC-00028 p300 IHC Antibody Hu IHC-P IF

FBXW7 A310-612A FBW7 AbVantage Pack Hu IP IHC-P IF

GLI3 A311-261A GLI3 AbVantage Pack Hu WB IP

GSK3B A310-723A GSK3-b AbVantage Pack Hu Ms WB IP PLA

HDAC1 A310-214A HDAC1 AbVantage Pack Hu Ms WB IP IHC-P IF PLA

HDAC2 A310-211A HDAC2 AbVantage Pack Hu Ms WB IP IHC IF ChIP

KIF3A A302-708A KIF3A Antibody Hu Ms WB IP

KIF7 A302-709A KIF7 Antibody Hu WB

MAP2K1 A310-761A MEK1 AbVantage Pack Hu WB IP IHC-P PLA

MAP2K1 A311-339A MEK1, phospho (T286)

AbVantage Pack

Hu IP

MED1 A300-793A CRSP1/TRAP220 Antibody Hu Ms WB IP IHC-P IF PLA

MED12 A300-774A MED12 Antibody Hu Ms WB IP IHC-P IF

MED23 A310-118A CRSP3 AbVantage Pack Hu WB IP

PRKAR1A A311-351A PRKAR1A AbVantage Pack Hu WB IP

PRKAR2A A301-670A PKA-RIIalpha Antibody Hu WB IP IHC-P IF

PRKCD A310-893A PKC-d AbVantage Pack Hu WB IP

RBBP4 A301-206A RbBP4 Antibody Hu Ms WB IP IHC-P IF PLA

RBBP7 A310-310A RbBP7 AbVantage Pack Hu Ms WB IP IHC-P IF PLA

SAP30 A303-551A SAP30 Antibody Hu Ms WB IP

SIN3A A310-219A Sin3A AbVantage Pack Hu Ms WB IP IHC-P IF PLA

STIL A310-885A SIL AbVantage Pack Hu Ms WB IP

SUFU A310-846A SuFu AbVantage Pack Hu WB IP

XPO1 A310-133A CRM1 AbVantage Pack Hu Ms WB IP IHC-P IF PLA

Detection of Human and Mouse SuFu by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Anti-bodies: Affinity purified rabbit anti-SuFu antibody A302-335A used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SuFu was also immunoprecipitated by rabbit anti-SuFu antibody A302-334A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of Human MEK1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti-MEK1 (Cat. No. A302-140A Lot1) used at a dilution of 1:1,000 (1 Detection: DAB)

Selected Product Citations

CDKN2A A300-340A, A301-267A: Wan G et al., Long non-coding RNA ANRIL (CDKN2B-AS) is induced by the ATM-E2F1 signaling pathway. Cell Signal. 2013 May;25(5):1086-95

MAP2K1 A302-140A: Eletr ZM et al., BAP1 is phosphorylated at serine 592 in S-phase following DNA damage. FEBS Lett. 2013 Dec 11;587(24):3906-11.

MED1 A300-793A: Verger A et al., The Mediator complex subunit MED25 is targeted by the N-terminal transactivation domain of the PEA3 group members. Nucleic Acids Res. 2013 Mar 26.

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