Which statement is not correct?

All enzymes are protein. All enzymes break chemicals apart. All enzymes are reuseable. All enzymes lower activation energy All enzymes have an active site.

Lactase enzyme testing

Lactase is supposed to digest the disaccharide LACTOSE into its two monomers, glucose and galactose.

Lactase can help prevent the discomfort associated with lactose intolerance.

Hydrolysis of Lactose

AND

LACTOSE

Glucose Galactose

Lactase enzyme causes Hydrolysis

AND

Glucose Galactose

Lactase enzyme

Glucose test stripsLACTOSE

Glucose Galactose

Glucose test strips do not respond to Lactose

Glucose test strips change color with Glucose

Let’s experiment with lactase

Is this enzyme specific? Can this enzyme “take the heat?”

Develop explanations based on evidence!

Does it digest lactose?

A limitation Your boss tells you that you must provide evidence

that is INDISPUTABLE or you are fired. Make sure that your tests leave no room for doubt.

Glucose test strips cost over $6000 each. You had better not use any more than you really need!

Today’s Lab

Please talk to your lab group and decide on the ONE procedure that your group will use

Divide responsibilities: One person reads directions aloud One person gathers equipment One person places liquid solutions in spot plate One person records the data