Print out this page, fill in and turn into your teacher. Name __________________________________

period ______ date______

Virtual Lab: Gel Electrophoresis

Go to: http://learn.genetics.utah.edu/content/labs/gel/

and follow instructions and fill out his lab sheet.

1. What is in the liquid in the tube in the first scene?

2. What technique do we use to sort and measure DNA?

3. This technique is used for other molecules like _____________________________.

4. What is the gel like that’s used?

5. Where do the DNA samples go?

6. How do we “push” DNA strands through the gel?

7. Explain how DNA samples sort themselves through the gel.

8. What do we do to make the DNA samples visible to our eyes?

9. What 6 things are need to make a gel?

10. What is agarose?

11. What is the buffer?

12. What 6 items are needed to load the DNA sample into the gel?

13. Which charge is closest to the wells in the electrophoresis box? (positive or negative, circle one) Does DNA have a positive or a negative charge? __________________ Knowing this and how the electrophoresis box is set up, why does the electric current have to be set up a certain way?

14. What appears in the electrophoresis box that tells us that the current is running?

15. In Step 5 of analyzing your DNA sample, you had to enter 3 size estimates. List here what you entered, from longest to shortest: _________________, ________________, ______________