veterinary diagnostic laboratory testing … booklet_2017.pdf7. non-necropsy diagnostic cases will...

VETERINARY DIAGNOSTICLABORATORY

TESTING INFORMATION

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Test offerings and prices are subject to change without notice. For the most up to date pricing information please contact the

University of Kentucky Veterinary Diagnostic Laboratory at 859-257-8283.

vdl.uky.edu

On our website you can find our current accession form, new account form and other testing submission forms.

vdl.uky.edu/laboratoryservices/submissionforms.aspx

Specimens become property of the UKVDL once received by the laboratory. Return of specimens to submitter is generally not permitted unless otherwise arranged.

Access to Case Reports and Billing Information can be 24/7:

UKVDL clients have the option of receiving clinical case reports via fax, email or hard copy (U.S. Postal Service).

We also offer 24/7 access to past and current case reports

as well as billing information via a web portal.

Email reporting and web access are the quickest and most reliable option that we offer.

For any changes or updates to your account, please call 859-257-8283.

The Business Office can assist you regarding changes to your case reporting

preferences and IT can assist you in establishing a web access account.

revised VDL Booklet_276885.indd 3

8/15/14 2:47 PM


Test offerings and prices are subject to change without notice. For the most up to date pricing information please contact the

University of Kentucky Veterinary Diagnostic Laboratory at 859-257-8283.

vdl.uky.edu

On our website you can find our current accession form, new account form and other testing submission forms.

vdl.uky.edu/laboratoryservices/submissionforms.aspx

Specimens become property of the UKVDL once received by the laboratory. Return of specimens to submitter is generally not permitted unless otherwise arranged.

Access to Case Reports and Billing Information can be 24/7:

UKVDL clients have the option of receiving clinical case reports via fax, email or hard copy (U.S. Postal Service).

We also offer 24/7 access to past and current case reports

as well as billing information via a web portal.

Email reporting and web access are the quickest and most reliable option that we offer.

For any changes or updates to your account, please call 859-257-8283.

The Business Office can assist you regarding changes to your case reporting

preferences and IT can assist you in establishing a web access account.


8/15/14 2:47 PM


Who we are and our commitment to you:

The University of Kentucky Veterinary Diagnostic Laboratory (UKVDL) is a nationally accredited full service animal health diagnostic laboratory. Our goal is to provide timely and accurate diagnostic laboratory support to promote animal health.

How we can help you:

Our state-of-the-art laboratory will assist you in diagnosing diseases or providing regulatory testing. We have one of the most accomplished pathology units in the country, ready to serve you by performing necropsies, reading biopsies, special stains, and other histological studies. Our clinical pathology section offers routine blood counts and chemistries, urinalysis, parasite identification, endocrinology, stone analysis and more. Our three microbiology sections can detect, isolate or identify veterinary pathogens of clinical importance and assist you in selecting the most appropriate antibiotic for treatment. They can also assist you in investigating and identifying emerging pathogens. Our toxicology section can detect, identify, and quantify numerous toxins and poisons that threaten animal health, including those found in plants and forage. Our serology section can measure antibody titers for many common diseases of equine, livestock and pets, and also provides a broad range of regulatory testing services to assist in the movement of animals to markets, import and export, shows, and special events. Our epidemiology section and ruminant extension veterinarian, in conjunction with all of our veterinary faculty and scientists, we can keep you informed regarding seasonal and other risks to animal health and provide consulting advice on how to detect and manage disease outbreaks. Finally, we can help you protect the public health through the detection of zoonotic diseases in animals.

Shipping (All Couriers): Diagnostic Services Coordinator: 1490 Bull Lea Road Deborah Maples, DVM Lexington, KY 40511 (859) 257-7112 [email protected] Mailing (via U.S. Postal Service): PO Box 14125 Ruminant Veterinarian: Lexington, KY 40512-4125 Michelle Arnold, DVM (859) 257-7190 (859) 257-8283 Phone [email protected] (859) 255-1624 Fax

CONTACT INFORMATION Hours: M-F 8-5 pm Sat 9-5 pm Sun 1-5 pm *After hours drop box available for lab specimens only


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UKVDL Services Conditions

1. A $10 accession fee will be assessed for all cases except:

Regulatory cases Cases assigned to Clinical Pathology and/or Toxicology only

2. A diagnostic case is defined as specimens submitted to VDL for diagnostic purposes on one or more

animals from one owner and residing at one premise. A complete history is requested to help our faculty and staff to provide the best diagnostic services for you and your client.

3. A regulatory case is defined as specimens submitted to fulfill legal requirements for the movement of

animals (e.g. import/export, interstate) and in support of state and federal surveillance, control and eradication efforts. The $10 accession fee will not apply to these cases (e.g. EIA, Piroplasmosis). The submitter must fill out regulatory forms completely and specimens clearly labeled with the animal’s id/name.

4. A necropsy case includes cases where the client has submitted an animal that has died for a complete

necropsy and ancillary testing to determine the cause of death. The price on our fee schedule for a necropsy is the total price for the case (not including the accession fee). However, a maximum of $200 of toxicological testing will be allowed on these cases. This cap may be waived by the Director or Associate Director on special cases. Any testing associated with a legal case will be billed in total (i.e. no capping).

5. All samples submitted to the VDL become the property of the laboratory unless agreed to by written

agreement prior to submission. 6. The VDL does not provide euthanasia services. We reserve the right to not accept live animals to assure the

safety of our employees. Please arrange for euthanasia before submission to VDL for a necropsy. 7. Non-necropsy diagnostic cases will be billed a per test charge for all tests. A “non-necropsy” case is any

case submitted to VDL that does not involve a necropsy. 8. Out-of-state submissions will be billed at 150% of In-State fees, except where noted in our fee schedule.

Toxicology tests are priced the same either In-State or Out-of-State. Animals residing on premises in Kentucky will be billed at the In-State rate.

9. Specimens referred to another laboratory will be assessed a $40 handling fee. 10. A fee of $25 will be assessed on packages that are leaking upon arrival at the laboratory. 11. The submitting veterinarian will be billed for all VDL services. Owners may submit animals for necropsy

with the approval of their veterinarian. In this instance, owners must pay for services at the time of specimen delivery. Payment cannot be accepted on Sundays. The UKVDL Receiving Section will contact the client for payment the week following services.

12. Services are billed monthly and payment is due upon receipt. The VDL reserves the right to inactivate

accounts when payment for services is not received in a timely manner.


8/15/14 2:47 PM


UKVDL Services Conditions

(As of July 1, 2014)

1. A $10 accession fee will be assessed for all cases except: Regulatory cases Cases assigned to Clinical Pathology and/or Toxicology only

2. A diagnostic case is defined as specimens submitted to VDL for diagnostic purposes on one or more

animals from one owner and residing at one premise. A complete history is requested to help our faculty and staff to provide the best diagnostic services for you and your client.

3. A regulatory case is defined as specimens submitted to fulfill legal requirements for the movement of

animals (e.g. import/export, interstate) and in support of state and federal surveillance, control and eradication efforts. The $10 accession fee will not apply to these cases (e.g. EIA, Piroplasmosis). The submitter must fill out regulatory forms completely and specimens clearly labeled with the animal’s id/name.

4. A necropsy case includes cases where the client has submitted an animal that has died for a complete

necropsy and ancillary testing to determine the cause of death. The price on our fee schedule for a necropsy is the total price for the case (not including the accession fee). However, a maximum of $200 of toxicological testing will be allowed on these cases. This cap may be waived by the Director or Associate Director on special cases. Any testing associated with a legal case will be billed in total (i.e. no capping).

5. All samples submitted to the VDL become the property of the laboratory unless agreed to by written

agreement prior to submission. 6. The VDL does not provide euthanasia services. We reserve the right to not accept live animals to assure the

safety of our employees. Please arrange for euthanasia before submission to VDL for a necropsy. 7. Non-necropsy diagnostic cases will be billed a per test charge for all tests. A “non-necropsy” case is any

case submitted to VDL that does not involve a necropsy. 8. Out-of-state submissions will be billed at 150% of In-State fees, except where noted in our fee schedule.

Toxicology tests are priced the same either In-State or Out-of-State. Animals residing on premises in Kentucky will be billed at the In-State rate.

9. Specimens referred to another laboratory will be assessed a $40 handling fee. 10. A fee of $25 will be assessed on packages that are leaking upon arrival at the laboratory. 11. The submitting veterinarian will be billed for all VDL services. Owners may submit animals for necropsy

with the approval of their veterinarian. In this instance, owners must pay for services at the time of specimen delivery. Payment cannot be accepted on Sundays. The UKVDL Receiving Section will contact the client for payment the week following services.

12. Services are billed monthly and payment is due upon receipt. The VDL reserves the right to inactivate

accounts when payment for services is not received in a timely manner.


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Test Name Specimen, Optimal AmountBold = preferred(Min. Amount)

Shipping Conditions Turn Around Time/Schedule

Test Comments In StateFee

Out State

Fee

MethodQL=qualQN=quant

Edit 2009 H1N1 Influenza Virus rRT-PCR (Real Time RT-PCR)

Swab from upper respiratory tract.

Contact the laboratory at 859-257-8283 before submitting any specimen not listed.

1 - 2 working days / MTWRF

The recommended specimen type to submit is a nasal swab obtained as early as possible following the onset of clinical signs submerged in a viral transport medium packed in ice.

$35.00 $52.50 Real-time PCR(QL)

Edit ACTH - Endogenous

1ml Frozen EDTA plasma

Collect blood in an iced or refrigerated EDTA tubes. Immerse in an ice bath immediately following collection. Separate the plasma from the cells and immediately freeze in plastic or silanized glass tubes. (Do not use nonsilanized glass) Stable up to 30 days at -20

1 working day / MTWRF Equine and Canine only $25.00 $37.50

Edit ACTH Stimulation 1 ml serum each sample

Same day if in lab by 3:00 pm. / MTWRF

2 samples for cortisol, Pre ACTH and Post ACTH.

$30.00 $45.00

Edit Aflatoxin 500 g feed (50 g) 250 ml milk (125 ml)

Sealed container or bag. Milk: Keep chilledForage: Chilled if sample is moist.

1-2 weeks / See Feed Sampling Guidelines for forage testing. Representative sampling is essential. Also see Mycotoxin panel.

Feed analyses include aflatoxin B1, B2, G1 and G2. Milk analyses include aflatoxin M1.

$50.00 $50.00 HPLC(QN)

Edit Albumin 1 ml serum (1 ml serum) clear unhemolyzed

Test is done daily if received by 3:00 PM. / MTWRF

$7.50 $11.25

Edit Alkaline phosphatase

1 ml. serum Serum, clear, unhemolyzed and separated.


$7.50 $11.25

Edit Alkaloid screen 50 g GI contents (10 g) 20 ml urine (10 ml) 25 g plant (10 g)

Sealed container. Frozen or chilled.

1-2 weeks / Screen includes: aconitine, anabasine, atropine, coniine, gamma-coniceine, 3,5-dimethoxyphenol (taxine marker), deltaline, nicotine, scopolamine

$60.00 $60.00 GC/MS(QL)

Edit Amycolatopsis PCR

Placenta, swab or bacterial isolates


1-2 working days / Twice a week

In suspected cases of abortion due to nocardioform placentitis, submit the entire fetus and placenta for laboratory examination. If submission of the entire fetus and placenta is not feasible, collect swab specimens of placental membranes into individual, leak-proof containers, clearly labeling each with animal ID and date of collection. The margins of visable lesions should be sampled. Swabs should be tested by both aerobic culture and PCR analysis.

$20.00 $30.00 PCR(QL)

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UK Veterinary Diagnostic Laboratory1490 Bull Lea RdLexington, KY 40511859-257-8283


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Out State

Fee


Edit Amylase 1 ml. serum: Clear, unhemolyzed serum is the preferred sample.


Specimens showing signs of bacterial contamination should not be tested. Specimens with elevated bilirubin levels may interfere with test.

$7.50 $11.25

Edit Anaplasma phagocytophilum/platys antibody ELISA

1.5 ml serum or 7 ml red top tube without separator gel. Note: Whole blood or plasma can also be used for testing.

Ship to the laboratory as soon as possible. Sample must be protected from extreme temperatures.

1 working day / Test is performed Monday -Friday.

Detects antibodies to Anaplasma phagocytophilum/platys. Anaplasmosis in dogs is a tickborne disease.

$15.00 $22.50

Edit Anaplasmosis cELISA

1.5 ml serum or 7 ml red top tube without separator gel.


1-2 working days / Test is performed on Mondays and Thursdays. Specimens need to be in receiving by 9:00 am for same day set up. Please contact serology if results are needed by a specific date.

In cases of suspected anaplasmosis, we recommend that a purple top tube be sent in at the same time for a CBC and differential.

$9.00 $13.50

Edit Anions in water 50 ml water (10 ml) Sealed container. Chilled.

2-5 business days / Panel includes bromide, chloride, fluoride, nitrate, nitrite, phosphate, and sulfate

$22.00 $22.00 IC(QN)

Edit Anticoagulant screen

20 g liver (5 g) Sealed container. 1-2 week / Screen includes: brodifacoum, bromadiolone, chlorophacinone, coumachlor, difethialone, diphacinone, warfarrin.

Dicoumarol is included for forage-eating species.

$150.00 $150.00 LC-MS/MS(QN)

Edit Arsenic 10 g liver (1 g) 10 ml urine (2 ml) 10 g kidney (1 g) 5 ml blood (1 ml) 25 g GI contents (5 g) 50 ml water (10 ml) 25 g source material (2 g)

EDTA tube for blood.Non-metal container for all others.Blood chilled.All others – frozen or chilled.

3-5 business days / Also see Metal panel. $20.00 $20.00 ICP-MS (QN)

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Out State

Fee


Edit Avian Influenza (NAHLN Methods)

Poultry oropharyngeal swab (OS) suspended in either 3-3.5 mL or 5-5.5 ml of brain heart infusion (BHI) broth . Up to 5 or 11 OS can bee pooled and suspended in 3-3.5 mL or 5-5.5 ml of BHI respectively. 1 wild bird cloacal swab suspended in 3-3.5 mL BHI (remove swab prior to transport). Entire tissue or alternatively 5 mm3 piece tissues added to 3.0 mL of BHI

The preferred specimen type is dependent upon species of bird as well as purpose, surveillance of highly pathogenic (HP) AI or low pathogenic (LP) AI. Poultry (single bird) oropharyngeal/tracheal swabs . Poultry (pool, up to 5 birds) oropharyngeal/tracheal swabs. Wild Bird cloacal swabs (no pools accepted, do not pool). Fresh Tissue from wild birds or poultry (no pools accepted, do not pool) spleen, lung, intestine

1-2 working days / MTWRF


Edit Avian Influenza Virus Antibody ELISA

1.5 ml of serum or 2-3 ml of blood in micro-collection tubes


1-2 working days / Test is performed daily, schedule varies according to workload. Please contact serology if results are needed by a specific date.

This test is also part of the NPIP program for flock certification/re certification, and specimens submitted for NPIP testing must have a completed VS 9-2 form.

$2.75 $4.12

Edit Avian Influenza Virus Antibody-AGID

1.5 ml serum or 2-3 ml of blood in a micro-collection tube.


2-3 working days / Test is performed Mon-Thurs, schedule varies according to workload. Please contact serology if results are needed by a specific date.

This test is part of the NPIP program for flock certification/recertification, and specimens submitted for NPIP must submit a completed VS 9-2 form. Please note: cutoff time for AGID testing is 12:00 pm. Samples received after this time will be set on the next work day. Results may take as long as 48 hours from date of setup.

$2.75 $4.12

Edit Babesia caballi cELISA



1 Working day from date of set up / Samples must be received by 8:30 am on day of testing to be included in the run. Test is performed on Tuesday and Thursday. Please contact Serology if results are needed by a specific date.

Detects antibodies to Babesia caballi in serum samples.

$16.00 $18.00

Edit Bile Acids 1 ml / Test is done daily if received by 3:00 PM/ MTWRF

Canine and Feline only $12.50 $18.75

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Out State

Fee


Edit Bilirubin, total 1 ml. Serum: Clear and unhemolyzed.


$7.50 $11.25

Edit Biopsy - Histopathology

NA Formalin fixed tissue in double-sealed container, with absorbent. Fresh tissue in leak-proof containers, specimens should be kept cool during transport. First two biopsies $50, each additional biopsy $20 each.

Tyically 24 - 48 hours; longer if additional stains, immunohistochemistry required / Mon - Fri

See Biopsy guidelines under Pathology section

$52.00 $78.00

Edit Blastomyces Antibody- AGID



2-3 working days from date of set up / Test is performed Monday-Friday.

Detects antibodies to Blastomyces dermatidis using agar gel immunodiffusion. Please note: cutoff time for AGID testing is 12:00 pm. Samples received after this time will be set on the next work day. Results may take as long as 48 hours from date of setup.

$7.00 $10.50

Edit Blister beetle ID Intact beetles (1 or more)

Sealed container or bag. 1 week / Do not crush. Can send fresh or preserved in alcohol

$10.00 $10.00 Visual ID(QL)

Edit Bluetongue cELISA 1.5 ml serum or 7 ml red top tube without separator gel.

Ship to the laboratory as soon as possible after collection. Protect the sample from extreme temperatures.

1 working day / Test is performed Tuesdays and Fridays. Samples must be in receiving by 9:00 am for same day set up. If results are need by a particular day please contact the laboratory.

Detects antibodies to Bluetongue virus using a competitive ELISA assay.

$6.00 $9.00

Edit Bluetongue Virus (BTV) real-time PCR

10 ml whole blood (EDTA), swab, tissue (spleen is the preferred organ, minimum 0.3 gr) or viral isolate

Please submit the specimens as soon as possible in cold conditions.

2 working days / MTWRF

This test has been developed to detect Bluetongue Virus (BTV) in clinical specimens.


Edit Bluetongue Virus (BTV) and Epizootic Hemorrhagic Disease Virus (EHDV) real-time PCR

10 ml whole blood (EDTA), swab, tissue (minimum 0.3 gr) or viral isolate


2 working day / MTWRF This test has been developed to detect both Bluetongue Virus (BTV) and Epizootic Hemorrhagic Disease Virus (EHDV) using a multiplex real-time PCR assay.


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Out State

Fee


Edit Botulism / Clostridium botulinum PCR - Sent to referral laboratory

50 g source material 25 g GI contents (stomach or rumen contents, feces) 10 ml serum

Sealed container.Chilled.

1-3 weeks / Sent to referral lab. Shipping charges apply.NOTE: We recommend senders ship samples directly to the referral laboratory, the Botulism Diagnostic Laboratory at the University of Pennsylvania (phone 610-925-6245), also called the National Botulism Reference Laboratory.

Suspected source material is often the best sample. Sort through source material for spoiled areas or evidence of carcasses. For birds, sera can be pooled from multiple birds. Testing serum is rarely diagnostic in horses.

$175.00 $175.00

Edit Bovine corona virus FA

Fresh Tissue: spiral colon and ileum (1 square inch of each tissue)

Keep specimen refrigerated and send in a leak proof container to the laboratory as soon as possible.

1 Working Day / MTWRF

$10.00 $15.00

Edit Bovine Leukemia Virus ELISA

1.5 ml serum 7 ml red top tube without separator gel.

Ship sample to the laboratory as soon as possible after collection. Protect sample from extreme temperatures.

Same day test is run / Samples must be received by 8:30 am on day of testing to be included in the run. Test is performed Tuesday and Fridays, results are available on same day as testing

Detects antibodies to the Bovine Leukemia Virus.

$6.00 $9.00

Edit Bovine Pregnancy Test (Ruminant) ELISA

7 ml red top tube or 1.5 ml serum (1.5 ml serum NOTE: for buffalo send in an EDTA tube)

Ship specimen to the laboratory as quickly as possible. Protect sample from extreme temperatures.

Samples must be received by 8:30 am on day of testing to be included in the run. Results are available on day of testing / Wednesdays

Samples must be received by 8:30 am on day of testing to be included in the run. Test detects pregnancy-associated glycoproteins in serum as a marker for pregnancy from 28 days post-breeding in cattle and goats,from 35 days post breeding in sheep, and from 30 days post breeding in buffalo. Test can be used at 60 days or greater post calving for cattle and buffalo. Currently there is no information available for post kidding in goats and sheep. “No diagnostic test is 100% accurate. Confirmation of pregnancy status by veterinary palpation or ultrasound is recommended.

$3.00 $4.50

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Out State

Fee


Edit Bovine respiratory disease- Bacterial Panel multiplex real-time PCR

Nasal or nasopharyngeal swabs, transtracheal washings, bronchoalveolar lavage fluid, affected lung, bacterial isolate. (10 gr affected lung)

Specimens should be submitted to the lab in leak-proof, sterile containers with refrigerated conditions as soon as possible.

1-2 working day. / Tuesdays and Thursdays

This panel has been implemented to detect major bacterial agents causing bovine respiratory disease complex. The bacterial agents in this panel includes Mycoplasma bovis, Mannheimia haemolytica, Histophilus somni and Pasteurella multocida. Because some of the bacterial respiratory pathogens could be present in healthy animals, the multiplex PCR results should be interpreted with clinical signs and other related laboratory findings. Bacterial culture and sensitivity can be performed upon request for an additional fee.


Edit Bovine respiratory disease-Viral and Bacterial Panel multiplex real-time PCR

Nasal or nasopharyngeal swabs, transtracheal washings, bronchoalveolar lavage fluid, affected lung tissue, bacterial or viral isolate (10 gr affected lung)


1-2 working day / Tuesdays and Thursdays

This panel has been implemented to detect major bacterial and viral pathogens causing bovine respiratory disease complex. The agents in this panel includes bovine corona virus (BCV), bovine herpes virus (BHV), bovine respiratory syncytial virus (BRSV) and bovine viral diarrhea virus (BVDV). Because some of the bacterial respiratory pathogens could be present in healthy animals, the multiplex PCR results should be interpreted with clinical signs and other related laboratory findings. Similarly, viral pathogens may be detected from intranasal vaccine administration. Bacterial culture and sensitivity can be performed upon request for an additional fee.


Edit Bovine respiratory disease-Viral Panel multiplex real-time PCR

Nasal or nasopharyngeal swabs, transtracheal washings, bronchoalveolar lavage fluid, affected lung tissue or viral isolate (10 gr affected lung)


1-2 working day / Tuesdays and Thursdays

Because viral pathogens may be detected following intranasal vaccine administration, the multiplex PCR results should be interpreted with clinical signs and other related laboratory findings.


Edit Bovine respiratory syncytial virus (RSV) VN

7 ml red top tube or 2 ml of serum

Separate serum from clot if possible. Do not send in Serum Separator Tube.

5 working days from set up date / R

Specimen must be in the laboratory by 2:00 pm Wednesdays to be included in Thursdays set up.

$7.00 $10.50

Edit Bovine respiratory syncytial virus (RSV) FA

Fresh Tissue: lung, trachea (1 square inch of each tissue)

Keep specimen refrigerated and send in a leak proof container to the lab as soon as possible.


$10.00 $15.00

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Out State

Fee


Edit Bovine rotavirus FA

Fresh Tissue: spiral colon and ileum (1 square inch of each tissue)



$10.00 $15.00

Edit Bovine viral diarrhea 1 VN


Separate serum from clot if possible. Do not send in serum separator tube.


Specimen must be in the laboratory by 2:00 pm Wednesdays to be included in Thursdays set up.Please note that both the BVD-1 and BVD-2 VN will be set unless special instructions are given.

$7.00 $10.50

Edit Bovine viral diarrhea 2 VN




Specimen must be in the laboratory by 2:00 pm Wednesdays to be included in Thursdays set up. Please note that both the BVD-1 and BVD-2 VN will be set unless special instructions are given.

$7.00 $10.50

Edit Bovine viral diarrhea virus FA

Fresh Tissue: lung, lymph node, kidney, spleen, and intestine (1 square inch of each tissue)


1 working day / MTWRF $10.00 $15.00

Edit Bovine viral Diarrhea Virus RT-PCR (Real Time PCR)

4-10 mL anticoagulated whole blood (EDTA blood), virus isolate, nucleic acid, ear notch, tissue (or scraping), milk, serum



For more information about the disease visit: http.bvdvinfo.org


Edit Brachyspira - Darkfield

4 in spiral colon 2 g of feces Spiral colon (preferred) or feces.

Spiral colon should be fresh (not fixed in formalin), less than 12 hours old when received by the Bacteriology Section, kept cool, and submitted in a labeled, large mouth container suitable for the size of the specimen. Feces should collected from the rectum (not the ground) and less than 12 hours old.

1 working day. / MTWRF

Cannot distinguish between B. hyodysenteriae (pathogen) and B. innocens (nonpathogenic).

$8.00 $12.00 Microscopy(QL)

Edit Bromethalin - currently sent to referral laboratory

10 g fat (5 g) 20 g brain (5 g) 20 g kidney (5 g) 10 g bait (5 g)

Sealed container.Frozen or chilled.

1-2 weeks / Currently sent to referral lab. Shipping charges apply.

$115.00 $115.00 LC-MS/MS(QL)

Edit Bromide, serum 2 ml serum (1 ml) Sealed container.Frozen or chilled.

1-3 business days / Separate serum from clot prior to shipping

$22.00 $22.00 IC(QN)

Edit Brucella abortus Card Agglutination

1.5 ml of serum or 7 ml red top without serum separator gel.

Ship to the laboratory as soon as possible. Protect sample from extreme temperatures.

1 working day / Test is run daily

$4.00 $6.00

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Out State

Fee


Edit Brucella abortus equine-agglutination




Detects antibodies to Brucella abortus (Fistulous withers) in equine serum samples.

$6.00 $9.00

Edit Brucella abortus plate test ( BAPA) agglutination


Ship specimens to the laboratory as soon as possible after collection. Protect samples from extreme temperatures.

1 working day / Test is performed Monday-Friday

$4.00 $6.00

Edit Brucella abortus standard plate test agglutination




$4.00 $6.00

Edit Brucella canis - agglutination



1 working day / Test is performed daily, results are available same day of testing.

Detects antibodies to Brucella canis in serum samples.

$15.00 $22.50

Edit Brucella melitensis card agglutination



1 working day / Test is performed Monday-Friday

$4.00 $6.00

Edit Brucellosis abortus antibody-Complement Fixation

1.5 ml serum or serum from 7 ml red top tube without separator gel. (1.5 ml of serum)


/ Test is performed at NVSL, Ames Iowa

Test is performed at NVSL, Ames, Iowa. In addition to the test fee there will be a charge for shipping sample to NVSL.

$18.00 $18.00

Edit BSE-Surveillance / $0.00 $0.00

Edit BUN 1 ml. Serum: Clear, unhemolyzed serum is the preferred sample.

Serum must be in the laboratory before 3:00 PM for same day turnaround. / MTWRF

$7.50 $11.25

Edit BVD,IBR,BRSV & PI-3 FA Profile

Fresh Tissue: lung, lymph node, kidney, spleen, and intestines (1 square inch of each tissue)



Edit BVDV Antigen ELISA

Ear Notch OR Serum (1 ml of serum/1 cm x 1 cm ear notch)

Refer to instructions on website for collection of ear notches. In case of a positive result, it is recommended that a follow up sample be submitted in 3 weeks.

3 working days from set up date / MTWRF

Serum is not an appropriate specimen for animals less than 3 months of age.

$4.50 $6.75

Edit Cadmium 10 g Liver (1 g) 10 g Kidney (1 g) 4 mL blood (1 mL) 25 g source material (5 g) 50 mL water (10 mL)

Non-metal container.Blood chilled.All other samples frozen or chilled.

3-5 business days / Also see Metal panel. $20.00 $20.00 ICP-MS (QN)

Edit Calcium 1 ml. Serum: Clear, unhemolyzed serum is the preferred specimen.

Specimen must be in the testing lab by 3:00 PM for same day analysis. / MTWRF

$7.50 $11.25

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Edit Calf Diarrhea Panel (Multiplex PCR)

5 gr fecal specimen (at least), intestinal scraping, bacterial isolate, nucleic acid

Specimens are submitted in a well labeled leak-proof container maintained at cold temperature during transport. Do not submit fecal specimens in gloves, screw-cap tubes are preferred.

2-3 days/Only run on Tuesdays and Thursdays. / 2-3 days/Only run on Tuesdays and Thursdays.

This is a multiplex PCR assay. These test results are intended to be used as an aid in the diagnosis of Bovine cornonavirus, Bovine rotavirus group A, E. coli K99, Salmonella, and Cryptosporidium. Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history. In case of positive results for Salmonella or E. coli, aerobic culture can be requested and antimicrobial susceptibility performed.


Edit Calf Diarrhea Panel (Multiplex PCR)

Fecal specimen (at least 5 gr), intestinal scraping, bacterial isolate, nucleic acid


2-3 days / Only on Tuesdays and Thursdays.

This is a multiplex PCR assay. These test results are intended to be used as an aid in the diagnosis of Bovine cornonavirus, Bovine rotavirus group A, E. coli K99, Salmonella, and Cryptosporidium. Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history. In case of positive results for Salmonella or E. coli, aerobic culture can be requested and antimicrobial susceptibility performed.


Edit Campylobacter fetus subspeciation PCR

Bacterial isolate. Contact the laboratory at 859-257-8283 before submitting any specimen not listed.


For more information about these two subspecies visit: http.microbewiki.kenyon.edu/index.php/Campylobacter_fetus

$30.00 $45.00 PCR(QL)

Edit Canine adenovirus FA

Fresh Tissue: lung, liver, kidney, spleen (1 square inch of each tissue)



Edit Canine corona virus FA

Fresh Tissue: intestine (1 square inch of tissue)



Edit Canine distemper virus FA

conjunctival smear, EDTA blood smear, urine OR Fresh Tissue: brain, lung, urinary bladder, stomach (1 square inch of each tissue)

Keep specimen refrigerated and send in a leak proof container as soon as possible.


Smears should be dry before sending to the laboratory.

$10.00 $15.00

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Edit Canine Heartworm Antigen Test -ELISA



1 working day. / Test is performed Monday-Friday.

Detect the presence of Canine heartworm antigen (Dirofilaria immitis )in serum or plasma

$15.00 $22.50

Edit Canine herpesvirus FA

Fresh Tissue: lung, liver, kidney, spleen, lymph nodes (1 square inch of each tissue)



$10.00 $15.00

Edit Canine influenza (H3N2) PCR

Swab from upper respiratory tract or lung tissue (20 gr)

The recommended specimen type to submit is a nasal swab submerged in a viral transport medium packed in ice

/ The specimen is referred to Wisconsin Veterinary Diagnostic Laboratory. Results are distributed to the client as soon as possible.

This is a referral test. Specimens are shipped to Wisconsin Veterinary Diagnostic Laboratory with an additional shipping fee.

$49.50 $49.50 PCR(QL)

Edit Canine Influenza Virus rRT-PCR (Real Time RT-PCR)

Swab from upper respiratory tract. The recommended specimen type to submit is a nasal swab submerged in a viral transport medium packed in ice.



This test detects all influenza A types. A second PCR could be done for H3N8 subtype. The recommended specimen type to submit is a nasal swab obtained as early as possible following the onset of clinical signs submerged in a viral transport medium packed in ice.


Edit Canine Pancreatic Panel

1 ml serum Test is run daily if received by 3:00 PM / MTWRF /

$30.00 $45.00

Edit Canine parainfluenza 2 FA

Fresh Tissue: lung, trachea (1 square inch of each tissue)



Edit Canine parvovirus FA

Fresh Tissue: intestine, spleen, thymus, heart, lymph node (1 square inch of each tissue)



Edit Canine Pregnancy Test ELISA

1.0 ml of serum or plasma, 3.0 ml of blood (1.0 ml of serum or plasma)

Ship to the lab as quickly as possible on ice. Results are available on the afternoon of testing / Test is performed daily

NOTE: samples must be tested within 4 hours of collection if stored at room temperature, and within 48 hours of collection if refrigerated.

$13.00 $19.50

Edit Canine Thyroid Panel

1 ml serum / This panel is run daily if received by 3:00 PM/ MTWRF

This panel includes Total T3, Total T4, Free T4 and TSH.

$45.00 $67.50

Edit Canine TLI 1 ml Serum - fasting minimum 6 hours

Test is done daily if received by 3:00 pm. / MTWRF

$20.00 $30.00

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Edit Canine Total T4 1 ml Serum Test is done daily if received by 3:00 pm. / MTWRF

$15.00 $22.50

Edit Canine TSH 1 ml Serum Test is done daily if received by 2:00 pm. / MTWRF

$15.00 $22.50

Edit Caprine Arthritis & Encephalitis cELISA



1 working day from date of set up. / Test is performed weekly, schedule varies according to workload. Please contact serology if results are needed by a specific date.

Detects antibodies to small ruminant Lentavirus (Caprine Arthritis and Encephalitis virus in goats and Ovine Progressive Pneumonia Virus in sheep).

$8.00 $12.00

Edit Caseous Lymphadenitis in small ruminants

2.0 ml of serum or a 7 ml red top tube without separator gel


Please allow at least 7 working days for the result. /

PLEASE NOTE: This test is sent to Washington Animal Disease Diagnostic Laboratory. There will be $10.00 accession fee and an additional cost for shipping, please contact the UK VDL for the most current pricing.

$11.25 $11.25

Edit CBC EDTA blood (1 ml) Specimen must be in the testing lab by 3:00 PM for same day analysis. 1 working day / MTWRF

$20.00 $30.00

Edit CBC (no differential)

EDTA blood (1 ml) Test is done daily if received by 3:00 PM. / MTWRF

$10.00 $15.00

Edit CBC Differential Only (manual)

2 to 4 peripheral smear of fresh, anticoagulated EDTA Blood.

Prepare smears by placing a drop of blood on a clean glass slide and pulling the drop out to a feathered edge.


RBC morphology is reviewed along with the differential cell count.

$10.00 $15.00

Edit Chemistry Panel 1 ml. Serum: Clear, unhemolyzed serum.


$20.00 $30.00

Edit Chemistry Panel- canine

1 ml. Serum: Clear and unhemolyzed.


$20.00 $30.00

Edit Chemistry Panel- equine



$20.00 $30.00

Edit Chemistry Panel- feline



$20.00 $30.00

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Edit Chemistry Panel- hepatic



$15.00 $22.50

Edit Chemistry Panel- porcine



$20.00 $30.00

Edit Chemistry Panel- renal



$15.00 $22.50

Edit Chemistry Panel-Ruminant

1 ml. Serum: Clear, unhemolyzed serum.


$20.00 $30.00

Edit Chemistry-eye fluid-Vit

1 ml. Eye fluid, vitreous Eye fluid, vitreous


$17.00 $25.50

Edit Chlamydiales PCR Isolate (liquid suspension), swab or wash, or at least 200 mg fresh or fixed tissue.



This procedure will detect veterinary pathogens within the order Chlamydiales. For more information visit: http://www.chlamydiae.com

$30.00 $45.00 PCR(QL)

Edit Chloride 1 ml. Serum: Clear, unhemolyzed and separated. Test may also be done on vitreous fluid, csf or other body fluids.


$7.50 $11.25

Edit Cholesterol 1 ml. Serum: Clear and unhemolyzed.


$7.50 $11.25

Edit Chromium 10 g liver (1 g) 10 g kidney (1 g) 4 mL blood (1 mL) 25 g source material (5 g) 50 mL water (10 mL)

EDTA tube for blood.Non metal container for all others.Blood chilled.All others frozen or chilled.

3-5 business days / Also see Metal panel $20.00 $20.00 ICP-MS (QN)

Edit Chronic Wasting Disease (CWD)

/ $60.00 $90.00

Edit CK (CPK) 1 ml. Serum: Clear and unhemolyzed.


$7.50 $11.25

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Edit Classical Swine Fever - CSF (NAHLN Testing)

CSF Sample Collection Instruction: CSF surveillance manual: http://www.aphis.usda.gov/vs/nahss/swine/csf/index.htm Fresh Tissue, tonsil (dead pigs), Nasal Swab (sick and dead pigs), Tonsil scraping

Any questions regarding sampling techniques can be directed to the USDA-APHIS-VS/NVSL-FADDL Diagnostic Services Section, Phone: (631) 332-3256 OR (631) 332-6814 (after business hours).



Edit Clostridium difficile (toxin A gene) PCR

1 isolate (plate Bacterial isolate, scraping or feces.



$25.00 $37.50 PCR(QL)

Edit Clostridium difficile (toxin B gene) PCR

1 isolate (plate culture or liquid suspension) Bacterial isolate, scraping or feces.



$25.00 $37.50 PCR(QL)

Edit Clostridium difficile Toxin A B ELISA

1 g feces or intestine contents. Feces or intestine contents.

Specimens should be kept cool and should be less than 24 hours old when received by the laboratory.

1 working day. / MTWRF

Fecal samples that have been preserved in formalin can not be used.

$15.00 $22.50 ELISA(QL)

Edit Clostridium FA 10 g fresh tissue. Fresh affected tissue; usually skeletal or heart muscle.

Sample of affected tissue should be fresh (not fixed in formalin), kept cool, and submitted in a labeled, large mouth container suitable for the size of the specimen.

2-3 working days. / MWF

$12.00 $18.00 Fluorescent Antibody (QL)

Edit Clostridium perfringens Toxin Typing PCR

Bacterial isolate (plate culture or liquid suspension)



The C. perfringens genotyping polymerase chain reaction (PCR) test is a qualitative assay designed to determine the genotype based on which of the different major toxin genes are present in an isolate – the test does not detect the presence of the toxin proteins.

$25.00 $37.50 PCR(QL)

Edit Clostridium piliforme PCR (Tyzzer's Disease)

( 200 mg) Fresh liver tissue


$35.00 $52.50 PCR(QL)

Edit Cobalt 10 g liver (1 g) 2 ml serum (1 ml) 2 ml whole blood (1 ml) 25 g feed (5 g)

Use non-metal container.Frozen or chilled.

3-5 business days / Also see Metal Panel, and Metal Panel-Trace Minerals

$22.00 $22.00 ICP-MS (QN)

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Edit Contagious Equine Metritis CF

1.5 ml serum or 7 ml red top tube without separator gel. Note: hemolyzed serum or plasma cannot be used for testing.


2 working days from date of set up. / Samples must be received by 1:30 pm on day of testing to be included in the run. Test is set up on Tuesdays, results available Wednesday afternoons.

A special form (KY-78 E) is required for all instate animals.

$18.00 $27.00

Edit Copper 10 g liver (1 g) 10 g kidney (1 g) 2 ml serum (1 ml) 50 g feed (5 g) 25 g source material (5 g) 50 ml water (10 ml) 50 mg liver biopsy (30 mg)

Non-metal container.Frozen or chilled.

3-5 business days / Liver and kidney should both be evaluated if toxicity is expected.Liver is the preferred sample for suspected deficiencies.If sending kidney, cortex is preferred.Also see Metal Panel and Metal Panel-Trace Minerals. See Metal Panel-Trace Minerals for more liver biopsy information.

$20.00 $20.00 ICP-MS (QN)

Edit Cortisol 1 ml Serum Test is done daily if received by 3:00 pm. / MTWRF

$15.00 $22.50

Edit Creatinine 1 ml. Serum: Clear and unhemolyzed.


$7.50 $11.25

Edit Crossiella equi PCR

Bacterial isolates, placenta or placental swabs.


1-2 working days / Twice a week

In suspected cases of abortion due to nocardioform placentitis, submit the entire fetus and placenta for laboratory examination. If submission of the entire fetus and placenta is not feasible, collect swab specimens of placental membranes into individual, leak-proof containers, clearly labeling each with animal ID and date of collection. The margins of visable lesions should be sampled. Swabs should be tested by both aerobic culture and PCR analysis.

$20.00 $30.00 PCR(QL)

Edit Cryptosporida 3 mL fresh feces specimen

/ 5 working days $15.00 $22.50

Edit Culture - Aerobic Swab of infected material (recommend Amies medium with charcoal), piece of tissue or organ, or fluid in leak-proof sealed clean or sterile container.

Deliver to laboratory as soon as possible after collection. Protect from hot and cold conditions that might destroy suspected pathogenic bacteria.

2 - 4 working days. / MTWRF

$19.00 $28.50 Culture(QL)

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Edit Culture - Anaerobic Aspirate of abscess or lesion in capped off syringe (expel air before sealing syringe and do not send with needle). Biopsy or tissue; if small must be maintained in an anaerobic transport system.

Do not freeze or cool samples for anaerobic culture. Transport at room temperature. Sample should not be over 48 hours old, preferably less than 4 hours old.


$20.00 $30.00 Culture(QL)

Edit Culture - Campylobacter species

0.2 - 0.3 g cervical mucus; 3 - 5 ml of semen, prepucial wash, or stomach contents; 1 - 3 g feces, 3 - 5 inches of tied-off intestine, or fecal or intestinal swab.

Specimen should be transported to the laboratory within 4 hours on ice packs.


$16.00 $24.00 Culture(QL)

Edit Culture - Contagious Equine Metritis

Swab from various sites of the genital tract of equine. Rayon tipped swabs of small diameter (.1 cm) must be used in collection from clitoral sinuses. Cervical or endometrial swabs must be collected with an appropriately sized guarded uterine swab and submitted in Amies charcoal medium.

Each swab must be submitted in Amies transport medium with charcoal and must be completely submerged in the medium. Swab must be processed within 48 hours after animal is cultured. If mailed, swabs must be submitted with ice packs. DO NOT FREEZE SAMPLES - Submit Cool on Frozen Gel Pacs. If swabs and ice packs are warm when received, the swabs will not be processed.

7 - 10 days. If more than 7 days of incubation is required, then the number of days of incubation required must be listed and highlighted by the submittor on the CEM accession form. / MTWRF

$27.00 $40.50 Culture(QL)

Edit Culture - Dermatophilus congolensis

1 cm x 1 cm (approximately) Small scab or crust Scabs and/or crust from edge of lesion.

Transport at room temperature in an envelope or other type of container that is not air tight.


$12.00 $18.00 Culture(QL)

Edit Culture - Fungal Few hairs or scales from lesion, 1 g tissue with lesion or a biopsy.

Room temperature transport and storage. Storage of DTM or other referral agar plates/slants in the refrigerator might cause condensation which could encourage overgrowth by saprophytic microorganism.


$21.00 $31.50 Culture(QL)

Edit Culture - Listeria Brain: 1 g tissue preferably the brain stem or pons area. Environmental samples: 10 - 20 g silage, taken from several areas.

Sample of affected tissue should be fresh (not fixed in formalin), should be kept cool and submitted in a labeled, large mouth container suitable for the size of the specimen. Silage can be transported at room temperature.


$16.00 $24.00 Culture(QL)

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Edit Culture - Microaerophilic

1 - 10 g fresh tissue. Swab in transport medium (recommend Amies medium with charcoal). Fluid, pieces of infected tissue or organ in leak-proof container.

Sample of affected tissue should be fresh (not fixed in formalin), should be refrigerated and submitted in a labeled, large mouth containter suitable for the size of the specimen.

7 - 10 days. / MTWRF $16.00 $24.00 Microscopy(QL)

Edit Culture - Mycobacterium sp.

1 - 2 g fresh tissue. Biopsy specimen or lesion in sterile container.

Sample of affected tissue should be fresh (not fixed in formalin), should be refrigerated and submitted in a labeled, large mouth containter suitable for the size of the specimen.

1 - 10 weeks. / MTWRF $20.00 $30.00 Culture(QL)

Edit Culture - Mycoplasma

1 - 2 g fresh tissue. Swab in transport medium. Fluid, pieces of infected tissue, or organ in leak-proof container.

Sample of affected tissue should be fresh (not fixed in formalin), should be kept cool and submitted in a labeled, large mouth containter suitable for the size of the specimen.

7 - 10 days. / MTWRF Synovial or joint fluid is usually culture negative, even on suspect cases.

$20.00 $30.00 Culture(QL)

Edit Culture - Ruminant Mastitis

(3 - 5 ml) Milk in sterile, leak-proof containers. Please do not submit in whirl-pak bags.

Best results are obtained if samples are chilled or placed on ice during transport to the laboratory. If more than 20 samples are to be submitted, the Bacteriology Section must be notified at least 1 week before they are submitted.


$8.00 $12.00 Culture(QL)

Edit Cyanide 25 g muscle (2 g) 25 g liver (2 g) 10 mL blood (2 mL) 100 g feed (10g) 500 mL water (100 mL) 50 g plant material (5 g) 50 g GI contents (5 g)

EDTA tube for blood .Sealed container or Whirlpak.Frozen in air tight container.

1-5 days. STAT same-day testing is available Mon-Fri upon request. /

Samples must be frozen in an airtight container as soon as possible and remain frozen until analyzed.Suspected feed (plant material) is the best source to test if availableInitial semi-quantitative analysis is done in-house; confirmatory testing is sent to referral lab if indicated

$20.00 $20.00 CYANTESMO paper(semi-QN)

Edit Cytology on submitted slides

Please submit labeled slides in a well padded, rigid container.

1-2 business days / Mon-Fri

$40 for 1-2 sites. $40 for each additional site, except in multiple aspirates of same disease process (e.g. multiple lymph nodes from lymphoma suspect; at the discretion of the pathologist).

$40.00 $60.00

Edit Cytology on submitted slides

Please submit labeled slides in a well padded, rigid container.

1-2 business days / Mon-Fri

$40 for 1-2 sites. $40 for each addition site, except in multiple aspirates of same disease process (e.g. multiple lymph nodes from lymphoma suspect; at the discretion of the pathologists.)

$40.00 $60.00

Edit Dexamethasone Suppression Test

1 ml serum each sample

Same day if in lab by 3:00 pm. / MTWRF

3 samples for cortisol, Pre Dex, 4 hour Post Dex and 8 hour Post Dex

$45.00 $67.50

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Edit DNA Sequence Analysis

Bacterial isolate (liquid suspension preferable)

Contact the laboratory at 859-257-8283 before submitting the specimen.


The Bacteriology section may request this test when an unusual or unknown bacterium is isolated from clinical specimens.

$100.00 $150.00 PCR(QL)

Edit Eastern Equine Encephalitis Virus RT-PCR

Mosquito pools, fresh tissues, nucleic acids



For information regarding arboviral disease visit: http://www.cdc.gov/ncidod/dvbid/Arbor/index.htm


Edit Ehrlichia canis/ewingii antibody by ELISA




Detects antibodies to Erlichia canis/ewengii.

$15.00 $22.50

Edit Electrolyte Panel 1 ml. Serum: Clear and unhemolyzed.


$12.00 $18.00

Edit Epizootic Hemorrhagic Disease AGID

1.5 ml serum or serum from 7 ml red top tube without separator gel.


2 - 3 working days from date of set up. / Test is performed Monday-Friday.

Detects antibodies to the virus that causes Epizootic Hemorrhagic Disease.Please note: cutoff time for AGID testing is 12:00 pm. Samples received after this time will be set on the next work day. Results may take as long as 48 hours from date of setup.

$5.00 $7.50

Edit Epizootic Hemorrhagic Disease virus (EHDV) real-time PCR

10 ml whole blood (EDTA), swab, tissue (spleen is the preferred organ, minimum 0.3 gr) or viral isolate


2 working days / MTWRF

This test has been developed to detect Epizootic Hemorrhagic Disease Virus (EHDV) in clinical specimens.


Edit Equine adenovirus FA

Fresh Tissue: lung (1 square inch of tissue)



Edit Equine Adenovirus PCR

Viral isolate, 200 mg fresh tissues including placenta, brain, spinal cord, lymph node, spleen and liver or fixed tissue, scraping or feces, upper respiratory tract swabs.

Contact the laboratory at 859-257-8283 or 257-7771 before submitting any specimen not listed. respiratory tract washes; feces, intestinal scrapings and fecal swabs


$30.00 $45.00 PCR(QL)

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Edit Equine Arteritis Virus rRT-PCR (EAV or EVA Real Time RT-PCR)

10 mL anticoagulated blood, swab, wash, 200 mg tissue, 10 mL seminal fluid (see test notes), aspirate from upper respiratory tract; Two specimens for each animal tested are recommended (see test notes).

Contact the laboratory at 859-257-8283 or 257-7771 before submitting any specimen not listed.


Live Animal Testing: To optimize laboratory confirmation of a diagnosis of equine arteritis virus (EAV) infection, it is recommended that a nasopharyngeal swab or washing and an unclotted blood sample be obtained as early as possible following the onset of clinical signs or in suspect cases of unapparent EAV infection. At least 10 mL of blood should be collected into EDTA (or ACD but not heparin) anticoagulant. In suspected cases of EVA-related abortion, submit the entire fetus and placenta for laboratory examination. If submission of the entire fetus and placenta is not feasible, collect specimens of placental membranes, lung, spleen, thymus and liver into individual, leak-proof containers, clearly labeling each with animal ID, tissue and date of collection. Where a stallion is a suspect carrier of EAV, he should be collected and 10 mL of semen containing the sperm-rich fraction of the full ejaculate submitted for testing. While the PCR test may be performed on seminal fluids, however, for regulatory purposes, such specimens may also need to be tested by virus isolation.


Edit Equine Diarrhea Panel (Multiplex PCR) - Bacterial Panel


Specimens are submitted in a well labeled leak-proof container maintained at cold temperature during transport. Do not submit fecal specimens in gloves, screw-cap tubes are preferred

/ 1-2 days/Only run on Tuesdays and Thursdays.

This is a multiplex Real-time PCR assay. These test results are intended to be used as an aid in the diagnosis of Lawsonia intracellularis, Salmonella spp and Neorickettsia risticii (agent of potomac horse fever). Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history. In case of positive results for Salmonella, aerobic culture can be requested and antimicrobial susceptibility performed.

$45.00 $45.00 PCR(QL)

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Edit Equine Diarrhea Panel (Multiplex PCR) - Bacterial Panel




This is a multiplex Real-time PCR assay. These test results are intended to be used as an aid in the diagnosis of Lawsonia intracellularis, Salmonella spp and Neorickettsia risticii (agent of potomac horse fever). Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history. In case of positive results for Salmonella, aerobic culture can be requested and antimicrobial susceptibility performed.

$45.00 $45.00 PCR(QL)

Edit Equine Diarrhea Panel (Multiplex PCR) - Combined (Bacterial and Viral) Panel

5 gr fecal specimen (at least), intestinal scraping, bacterial or viral isolate, nucleic acid



This is a multiplex Real-time PCR assay. These test results are intended to be used as an aid in the diagnosis of Lawsonia intracellularis, Salmonella spp, Neorickettsia risticii (agent of potomac horse fever), equine rota virus and equine corona virus. Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history. In case of positive results for Salmonella, aerobic culture can be requested and antimicrobial susceptibility performed.

$80.00 $80.00 PCR(QL)

Edit Equine Diarrhea Panel (Multiplex PCR) - Combined (Bacterial and Viral) Panel

5 gr fecal specimen (at least), intestinal scraping, bacterial or viral isolate, nucleic acid



This is a multiplex Real-time PCR assay. These test results are intended to be used as an aid in the diagnosis of Lawsonia intracellularis, Salmonella spp, Neorickettsia risticii (agent of potomac horse fever), equine rota virus and equine corona virus. Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history. In case of positive results for Salmonella, aerobic culture can be requested and antimicrobial susceptibility performed.

$80.00 $80.00 PCR(QL)

Edit Equine Diarrhea Panel (Multiplex PCR) - Viral Panel

5 gr fecal specimen (at least), intestinal scraping, viral isolate, nucleic acid



This is a multiplex Real-time PCR assay. These test results are intended to be used as an aid in the diagnosis of equine rota virus and equine corona virus. Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history.

$45.00 $45.00 PCR(QL)

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Edit Equine Diarrhea Panel (Multiplex PCR) - Viral Panel

5 gr fecal specimen (at least), intestinal scraping, viral isolate, nucleic acid



This is a multiplex Real-time PCR assay. These test results are intended to be used as an aid in the diagnosis of equine rota virus and equine corona virus. Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history.

$45.00 $45.00 PCR(QL)

Edit Equine Diarrhea, Adult Diagnostic Plan

Minimum 3 separate 50 gr fresh feces in leak proof container ( for aerobic culture, Salmonella PCR, PHF PCR, Lawsonia PCR and fecal exam); Minimum 10 ml EDTA whole blood (for PHF PCR) ; and fecal swab placed in an anaerobic transport media (BBL Port-a-cul) (for anaerobic culture).

Fecal specimens should be freshly collected and submitted to the lab in cold chain. Fecal swab for anaerobic culture should be submitted in anaerobic transport media since anaerobic bacteria are very susceptible to air (oxygen).

2-3 days for PCR tests (Salmonella, PHF and Lawsonia), aerobic culture and fecal exam. Anaerobic culture may take up to 10 days. /

UKVDL has developed this problem-based panel to assist practitioners with a focused diagnostic test selection that should increase the likelihood of arriving at an accurate diagnosis. The panel includes Aerobic culture (includes susceptibility) - Anaerobic culture (including susceptibility) - Fecal Exam - Salmonella PCR - Potomac Horse Fever (PHF) PCR - Lawsonia PCR, if needed.

$140.00 $140.00 Varies

Edit Equine Diarrhea, Foal Diagnostic Plan

Samples needed:a- Minimum 3 separate 50 gr fresh feces in leak proof container (for Aerobic culture , Salmonella PCR, Lawsonia PCR, Cl. difficile toxin ELISA, fecal exam), b- Fecal swab placed in anaerobic transport media (BD, BBL™ Port-a-cul™) (for anaerobic culture) c- EDTA whole blood (LTT) (for Selenium)

Fecal specimens should be freshly collected and submitted to the lab in cold chain. Fecal swab for anaerobic culture should be submitted in anaerobic transport media since anaerobic bacteria are very susceptible to air (oxygen).

2-3 days for PCR tests (Salmonella and Lawsonia), Clostridium difficile toxin ELISA, Selenium, aerobic culture and fecal exam. Anaerobic culture may take up to 10 days. / MTWRF

UKVDL has developed this problem-based panel to assist practitioners with a focused diagnostic test selection that should increase the likelihood of arriving at an accurate diagnosis.This panel includes following tests:- Aerobic bacterial culture (includes susceptibility) - Anaerobic bacterial culture including susceptibility - Fecal exam - Clostridium difficile toxin ELISA -Lawsonia PCR - Salmonella PCR - Selenium.

$145.00 $145.00 Varies

Edit Equine herpes virus 1 VN



3 working days from set up date. / T

Specimen must be in the laboratory by 2:00 pm on Monday.

$15.00 $22.50

Edit Equine herpes virus FA

Fresh Tissue: lung, liver, spleen, thymus, kidney (1 square inch of each tissue)



$10.00 $15.00

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Edit Equine Herpesvirus 1 Detection (Real Time PCR-charged per animal)

10 mL blood, swab, wash, or aspirate, 200 mg tissue; 2, 20 um slices from a paraffin embedded tissue block. Tissues include placenta, brain stem, brain, spinal cord, and lymph node Nucleic acid, Viral isolate; Swab, Wash, or aspirate from upper respiratory tract; anticoagulated whole blood (EDTA blood)..

Contact the laboratory at 859-257-8283 or 257-7771 before submitting any specimen not listed.


Live Animal Testing: To optimize laboratory confirmation of a diagnosis of equine herpesviris 1 (EHV1) infection, it is recommended that both a nasopharyngeal swab or washing and an unclotted blood sample be obtained as early as possible following the onset of clinical signs or in suspect cases of unapparent EHV infection. At least 10 mL of blood should be collected into EDTA (or ACD but not heparin) anticoagulant. Special equine nasal swab sampling kits are now available through Gluck Equine Research Center, University of Kentucky (859-257-4757 ext. 81124). The swabs in these kits are 16 inches in length, a size not available from most distributors. The charge per animal for EHV1 PCR testing will be the same whether submitting a single specimen or the recommended pair of specimens. Postmortem Testing: For animals exhibiting signs suggestive of neurological herpesvirus submit well labeled fresh tissue, especially brain stem and submandibular lymph node tissue. For suspected EHV1 abortions submit the entire fetus and membranes. If not practical submit well labeled fresh tissue samples. The EHV1 pathotyping test is automatically performed on samples found to be positive using the EHV1 PCR test procedure.


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Edit Equine Herpesvirus 1 Pathotyping PCR (run only if Equine Herpesvirus 1 Detection PCR result is Positive)

10 mL blood, 1 swab, wash, or aspirate, (200 mg tissue), (2, 20 um slices from a paraffin embedded tissue block.) Tissues include plac

veterinary diagnostic laboratory testing … booklet_2017.pdf7. non-necropsy diagnostic cases will...

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