v cadot,: 2nd wp coordinators meeting leafy vegetables, austria, 1 july 2008 wp meeting leafy...
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V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
WP meeting Leafy Vegetables WP meeting Leafy Vegetables
WP 3WP 3Cadot Valérie
GEVES :Variety and seed, study and control group
Groupe d’Etude et de contrôle des Variétés Et des Semences
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
A.A. ObjectivesObjectives
Evaluation for pests and diseases resistanceEvaluation for pests and diseases resistance
Evaluation for quality and abiotic charactersEvaluation for quality and abiotic characters
Inclusion in the databasesInclusion in the databases
to enable a more sustainable production methods and a to enable a more sustainable production methods and a
better quality productbetter quality product
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
DeliverablesDeliverables for WP3 for WP3Deliverable dateWP3
Deliverable descriptionStatus Partner
responsible
Year 1
D16; month 12
Seeds of the selected material of the four crop groups sent to the partners involved
Done P0, P1, P2, P3, P4, P5, P7, P8, P9, P10, P12, P13
Year 4
D17; month 45 115 accessions of lettuce evaluatedNot yet due P1, P4, P7,
P9, P10, P13
D18; month 45 320 accessions of spinach evaluated Not yet due P0, P5
D19; month 45 265 accessions of chicory evaluated Not yet due P2, P8, P12
D20; month 45 49 accessions of minor crops evaluatedNot yet due P3, P5, P9,
P12
D21; month 45Evaluation data entered in a fixed format to
be sent to the database managers
Not yet due P0, P1, P2, P3, P4, P5, P7, P8, P9, P10, P12, P13
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Milestones for WP3Milestones for WP3Milestone date
Milestone description Status Partner responsible
Year 1
month 12Material selected for the evaluation activities of the four crop groups (see WP1)
Done Pall
Year 2
month 18 Evaluations started
On going P0, P1, P2, P4, P5, P7, P8, P9, P10, P12, P13
Year 3
month 36 70 accessions of lettuce evaluatedNot yet due P1, P4, P7,
P9, P10, P13
month 36 210 accessions of spinach evaluated Not yet due P0, P5
month 36 170 accessions of chicory evaluated Not yet due P2, P8, P12
month 36 30 accessions of minor crops evaluatedNot yet due P3, P5, P9,
P12
Year 4 See deliverables Not yet due
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
EvaluationEvaluationforfor pests and diseases resistancepests and diseases resistance
Lettuce: Lettuce: 52 accessions52 accessions
– Bremia lactucaeBremia lactucae : : P4 (CZ): 30 with 20 isolates by seedling tests, P4 (CZ): 30 with 20 isolates by seedling tests, P10 (GBR): 17 accessions for field resistanceP10 (GBR): 17 accessions for field resistance
– Root-knot nematodes (Root-knot nematodes (Meloidogyne spMeloidogyne sp.).)::P13 (SLO): 10-20P13 (SLO): 10-20 Spinach: Spinach: 250 accessions (3-5 races)250 accessions (3-5 races)
– Downy mildew (Peronospora farinosa)Downy mildew (Peronospora farinosa) P0 (NLD) : 250P0 (NLD) : 250
Chicory: Chicory: 165 accessions165 accessions – Thielaviopsis basicolaThielaviopsis basicola P2 (FRA): 75 P2 (FRA): 75 – Alternaria cichoriiAlternaria cichorii P2 (FRA): 60P2 (FRA): 60– Sclerotinia sclerotiorum Sclerotinia sclerotiorum P2 (FRA): 30 P2 (FRA): 30
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
EvaluationEvaluationfor quality and abiotic charactersfor quality and abiotic characters
Lettuce: Lettuce: 55 accessions55 accessions– Post harvest discolorationPost harvest discoloration P1(GBR): 24P1(GBR): 24– Winter hardinessWinter hardiness P7 (AUT): P7 (AUT): 1515
Spinach: Spinach: 70 accessions70 accessions– Nitrate, oxalic acid, dry matterNitrate, oxalic acid, dry matter P5 (DAN, SW)P5 (DAN, SW)
Chicory: Chicory: 225 accessions225 accessions– Shape, color, taste, history, regionalismShape, color, taste, history, regionalism P8 (CHE): P8 (CHE): – SesquiterpenesSesquiterpenes P12 (ITA): 100P12 (ITA): 100
Rucola: Rucola: 40 accessions40 accessions– Nitrate, dry matter Nitrate, dry matter P5 (DAN, SW): 20P5 (DAN, SW): 20– Glucosinolate contentGlucosinolate content P12 (ITA): 20 P12 (ITA): 20
to 40to 40
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Evaluation for P0 (CGN)Evaluation for P0 (CGN)
What has been done: What has been done: Start made of Start made of the evaluation of 263 spinach the evaluation of 263 spinach accessions for 2 accessions for 2 Peronospora Peronospora farinosafarinosa pathotypes. pathotypes.
What will be done: What will be done: Finalize the Finalize the evaluations experiments and evaluations experiments and include the data in the common include the data in the common database.database.
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Evaluation for P1 : (WHRI)Evaluation for P1 : (WHRI) What has been done:What has been done:
The selected material of The selected material of lettucelettuce has been has been grown in an experimental field plot, grown in an experimental field plot, harvested under controlled conditions harvested under controlled conditions and and screened for post harvest colourationscreened for post harvest colouration following established experimental following established experimental protocols. protocols. The The analysis of the dataanalysis of the data has been carried has been carried out.out.
What will be done: What will be done: Data to be provided to Data to be provided to lettuce data base manager and WP lettuce data base manager and WP coordinatorcoordinator
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Evaluation for P2 Evaluation for P2 (GEVES)(GEVES)
What has been done:What has been done:P2 startedP2 started the evaluation of disease the evaluation of disease resistance:resistance:- 14 accessions to - 14 accessions to Sclerotinia sclerotiorum Sclerotinia sclerotiorum (by FNPE). (by FNPE). - 25- 25 accessions to accessions to Thielaviopsis basicolaThielaviopsis basicola- 27 accessions - 27 accessions to to Alternaria cichoriiAlternaria cichorii
What will be done: What will be done: finalise the evaluation finalise the evaluation
experimentations andexperimentations and enter the data in enter the data in the chicory databasethe chicory database
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Evaluation for P4 Evaluation for P4 ((Palacky)Palacky)
What has been done: What has been done: Selection of 30 Selection of 30 lettuce accessions for characterization.lettuce accessions for characterization.Control of viability and pathogenicity of Control of viability and pathogenicity of races of races of Bremis lactucae, Bremis lactucae, pre-selection of pre-selection of isolates to be used for evaluation in a isolates to be used for evaluation in a year 2.year 2. Evaluation of Lactuca sativa Evaluation of Lactuca sativa accessions for response to Bremia accessions for response to Bremia lactuca isolates started in year 2lactuca isolates started in year 2
What will be done: What will be done: Evaluation of lettuce Evaluation of lettuce accessions for response to 10 isolates of accessions for response to 10 isolates of Bremia lactucae Bremia lactucae will continuewill continue
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Evaluation for P5 (Nordgen)Evaluation for P5 (Nordgen)
What has been done: What has been done: - - RucolaRucola (Eruca) material for chemical (Eruca) material for chemical evaluations is planted.evaluations is planted.- - SpinachSpinach growing was postponed to growing was postponed to August due to high risk of bolting in the August due to high risk of bolting in the middle of summer.middle of summer.
What will be done: What will be done: Chemical evaluations Chemical evaluations of 60 spinach, 5 orache (Atriplex) and 20 of 60 spinach, 5 orache (Atriplex) and 20 rucola accessions will start in autumn rucola accessions will start in autumn 2008.2008.
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Evaluation for P7 (Evaluation for P7 (Arche Noah)Arche Noah)
What has been done: What has been done: First selection First selection of accessions to be tested for winter of accessions to be tested for winter hardiness. Criteria for evaluating hardiness. Criteria for evaluating winter hardiness has been winter hardiness has been developed.developed.
What will be done:What will be done:
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Evaluation for P10 Evaluation for P10 ((HDRAHDRA))
What has been done: What has been done: Material for Material for evaluation (lettuce) already evaluation (lettuce) already selected (all 17 Heritage Seedselected (all 17 Heritage Seed Library accessions).Library accessions).
What will be done: What will be done: Nothing planned Nothing planned until year 2until year 2
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Evaluation for P12 Evaluation for P12 ((University of Bologna)University of Bologna)
What has been done: What has been done: 30 accessions30 accessions of Erucaof Eruca received from P3. received from P3. 3 controls3 controls received from P1 (1 Eruca, 2 Diplotaxis; received from P1 (1 Eruca, 2 Diplotaxis; 3 local controls added3 local controls added = Eruca, 2 Diplotaxis and 1 Diplotaxis = Eruca, 2 Diplotaxis and 1 Diplotaxis The plantlets were transplanted at Experimental farm, Cesena, The plantlets were transplanted at Experimental farm, Cesena, on April 30, on 4-rows, single replication plots. on April 30, on 4-rows, single replication plots.
The harvest of leaves for The harvest of leaves for glucosinolate evaluationglucosinolate evaluation is forecasted is forecasted for the first week of June. Since glucosinolate profile may for the first week of June. Since glucosinolate profile may change both with climatic conditions and ontogenetic change both with climatic conditions and ontogenetic development, all accessions were harvested in a reduced time development, all accessions were harvested in a reduced time span, at the pre-flowering stage (flower buds removed from the span, at the pre-flowering stage (flower buds removed from the earlier accessions).earlier accessions).
As the material looked very interesting, a focus group was As the material looked very interesting, a focus group was organised to discuss about the organised to discuss about the perceived sensory charactersperceived sensory characters that could be worth evaluation that could be worth evaluation on rocketon rocket. The group met in . The group met in Cesena, on 13 and 28 May, in two 2-hour sessions. This activity Cesena, on 13 and 28 May, in two 2-hour sessions. This activity could have a follow up at harvestcould have a follow up at harvest. .
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Evaluation for P12 Evaluation for P12
Bottlenecks: Bottlenecks: none, with respect to none, with respect to Eruca / Diplotaxis. Because of the Eruca / Diplotaxis. Because of the shortage of personnel and the delay shortage of personnel and the delay in refunding, it was decided not to in refunding, it was decided not to overlap activities, in order to prevent overlap activities, in order to prevent the risk of not being able to carry on the risk of not being able to carry on the work. the work.
So, it was decided to postpone the So, it was decided to postpone the activities on chicory.activities on chicory.
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Evaluation for P12Evaluation for P12
V Cadot,: 2nd WP coordinators meeting Leafy Vegetables , Austria, 1 July 2008
Evaluation for P13 (KIS)Evaluation for P13 (KIS)
What has been done: What has been done: Preparation of Preparation of the material to be evaluated, the material to be evaluated, preparation of inoculums for preparation of inoculums for screening for tolerance-resistance screening for tolerance-resistance to nematodes.to nematodes.
What will be done: What will be done: Evaluation of the Evaluation of the selected material for nematode selected material for nematode resistance/tolerance.resistance/tolerance.