p coquin : 3rd wp coordinators meeting leafy vegetables, warwick, 11 june 2009 wp meeting leafy...

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P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 2009 WP meeting Leafy Vegetables WP meeting Leafy Vegetables WP 3 WP 3 Pascal COQUIN GEVES : Variety and seed, study and control group Groupe d’Etude et de contrôle des Variétés Et des Semences

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Page 1: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 2009

WP meeting Leafy Vegetables WP meeting Leafy Vegetables

WP 3WP 3Pascal COQUIN

GEVES :Variety and seed, study and control group

Groupe d’Etude et de contrôle des Variétés Et des Semences

Page 2: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 200921/04/2321/04/23

A.A. ObjectivesObjectives

Evaluation for pests and diseases resistanceEvaluation for pests and diseases resistance

Evaluation for quality and abiotic charactersEvaluation for quality and abiotic characters

Inclusion in the databasesInclusion in the databases

to enable a more sustainable production methods and a to enable a more sustainable production methods and a

better quality productbetter quality product

Page 3: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 2009

DeliverablesDeliverables for WP3 for WP3Deliverable dateWP3

Deliverable descriptionStatus Partner

responsible

Year 1

D16; month 12Seeds of the selected material of the four

crop groups sent to the partners involved

Done except for P12

P0, P1, P2, P3, P4, P5, P7, P9, P10, P12, P13

Year 4

D17; month 45 115 accessions of lettuce evaluatedNot yet due P1, P4, P7,

P9, P10, P13

D18; month 45 320 accessions of spinach evaluated Not yet due P0, P5

D19; month 45 265 accessions of chicory evaluated Not yet due P2, P12

D20; month 45 49 accessions of minor crops evaluated Not yet due P3, P5, P12

D21; month 45Evaluation data entered in a fixed format to

be sent to the database managers

Not yet due P0, P1, P2, P3, P4, P5, P7, P9, P10, P12, P13

Page 4: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 2009

Milestones for WP3Milestones for WP3Milestone date

Milestone description Status Partner responsible

Year 1

month 12Material selected for the evaluation activities of the four crop groups (see WP1)

Done except for P12

P0, P1, P2, P3, P4, P5, P7, P9, P10, P12, P13

Year 2

month 18 Evaluations started

On going P0, P1, P2, P4, P5, P7, P9, P10, P12, P13

Year 3

month 36 70 accessions of lettuce evaluatedNot yet due P1, P4, P7,

P9, P10, P13

month 36 250 accessions of spinach evaluated Not yet due P0, P5

month 36 170 accessions of chicory evaluated Not yet due P2, P12

month 3630 accessions of minor crops evaluated

Not yet due P3, P5, P12

Year 4 See deliverables Not yet due

Page 5: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 2009

Milestones in 2010 of evaluationMilestones in 2010 of evaluationfor pests and diseases resistancefor pests and diseases resistance

Lettuce:Lettuce: 60 accessions60 accessions

– Bremia lactucaeBremia lactucae : : P4 (CZ): P4 (CZ): 30 accessions with 20 isolates30 accessions with 20 isolates by seedling by seedling

tests, tests, P9 (GBR): P9 (GBR): 17 accessions17 accessions for field resistance for field resistance

– Root-knot nematodes (Root-knot nematodes (Meloidogyne spMeloidogyne sp.).)::P10 (SLO): P10 (SLO): 10-2010-20 Spinach:Spinach: 250 accessions250 accessions (3-5 races) (3-5 races)

– Downy mildew (Peronospora farinosa)Downy mildew (Peronospora farinosa) P0 (NLD) : 250P0 (NLD) : 250

Chicory:Chicory: 165 accessions165 accessions – Alternaria cichoriiAlternaria cichorii P2 (FRA): P2 (FRA): 6060– Sclerotinia sclerotiorum Sclerotinia sclerotiorum P2 (FRA): P2 (FRA): 3030 – Thielaviopsis basicolaThielaviopsis basicola P2 (FRA): P2 (FRA): 7575

Page 6: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 2009

Milestones in 2010 of evaluation Milestones in 2010 of evaluation for quality and abiotic charactersfor quality and abiotic characters

Lettuce:Lettuce: 45? (or less)45? (or less) instead of instead of 55 accessions?55 accessions?– Post harvest discolorationPost harvest discoloration P1(GBR): 24P1(GBR): 24– Winter hardinessWinter hardiness P7 (AUT): 6 instead of P7 (AUT): 6 instead of

1515– TasteTaste P7 (AUT): 6P7 (AUT): 6

Spinach:Spinach: 70 accessions70 accessions– Nitrate, oxalic acid, dry matterNitrate, oxalic acid, dry matter P5 (DAN, SW)P5 (DAN, SW)

Chicory:Chicory: 100 accessions100 accessions– SesquiterpenesSesquiterpenes P9 (ITA): 100P9 (ITA): 100

Rucola:Rucola: 49 accessions49 accessions– Nitrate, dry matter Nitrate, dry matter P5 (DAN, SW): 20P5 (DAN, SW): 20– Glucosinolate contentGlucosinolate content P9 (ITA): 20 to 40P9 (ITA): 20 to 40

Page 7: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 2009

WP3 : what has been done in 2008WP3 : what has been done in 2008Pests & diseases resistancePests & diseases resistance

+ Quality & abiotic characters evaluation+ Quality & abiotic characters evaluation

0%

20%

40%

60%

80%

100%

120%

140%

Lettuce Spinach Chicory Minor crops

Achieved numbers of accessions in 2008 in % of the total scheduled P10

P9

P8

P7

P5

P4

P3

P2

P1

P0

!

7/147/14

16/1716/17

28/2428/24

30 acc. 30 acc. 3/20 str.3/20 str.

6/66/6

51/7051/70

263/250263/250

20%20% (53/265) (53/265)

3737

2020

136%136% (57/49) (57/49)

83% (83% (87/10587/105))

99%99% ( (318/320318/320))

Page 8: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 2009

WP3 : what will be done in 2009WP3 : what will be done in 2009Pests & diseases resistance + Pests & diseases resistance +

Quality & abiotic characters evaluationQuality & abiotic characters evaluation

To evaluate in 2009Total

2008+2009

Objective 2008+200

9

To evaluate in 2010

Objective 2010

Pests & Diseases

5330 acc. for 17/20 B.l. by P4 and 7 by P13

124>

Quality 34 0Pests &

Diseases263 0 333>

Quality 55 15 by P5

Pests & Diseases

5367 =20 ad+16ss+31 tv

by P2170=

95 of which 45(18ac+27tv

) by P2

Quality 0 50 by P12and 50 by

P12

Pests & Diseases

0 67>

Quality 6749

Done in 2008

87

318

53

67Minor crops

70

210

170

30

Lettuce

Spinach

Chicory

0

0

00

115

320

265

105 105 inst. inst. of 115of 115

P4: 30 P4: 30 acc. ? str. acc. ? str. of B.l.?of B.l.?

5757

57 >57 >

Page 9: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 200921/04/2321/04/23

Evaluation for P0 (CGN)Evaluation for P0 (CGN)

What has been done :What has been done :- Evaluation of - Evaluation of 263 spinach accessions263 spinach accessions for 2 for 2 P. P. farinosafarinosa pathotypes resistance (Pfs 5 and Pfs 7) pathotypes resistance (Pfs 5 and Pfs 7)

- Send the results of Pfs5 and Pfs7 to WP3 leader - Send the results of Pfs5 and Pfs7 to WP3 leader at the end of 2008at the end of 2008

- Include the data in the common database,- Include the data in the common database,

What will be done in 2009 :What will be done in 2009 :- Evaluation of the 263 spinach accesions for 2 - Evaluation of the 263 spinach accesions for 2 other other P. Farinosa P. Farinosa pathotypes resistance (Pfs1 pathotypes resistance (Pfs1 and Pfs 4)and Pfs 4)

Page 10: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 200921/04/2321/04/23

Evaluation for P1 : (WHRI)Evaluation for P1 : (WHRI)

What has been done :What has been done : In 2007: evaluation of In 2007: evaluation of 28 lettuce 28 lettuce accessionsaccessions for for 2 types of discoloration : 2 types of discoloration : browning and pinkingbrowning and pinking (> 24 in the (> 24 in the The The analysis of the data analysis of the data has been carried has been carried out.out.

What will be done in 2009 : What will be done in 2009 : Data to be Data to be provided to lettuce database manager and provided to lettuce database manager and WP coordinatorWP coordinator

Page 11: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 200921/04/2321/04/23

Evaluation for P2 Evaluation for P2 (GEVES)(GEVES) What has been done :What has been done :

Evaluation of disease resistance :Evaluation of disease resistance :- 14 accessions to - 14 accessions to Sclerotinia sclerotiorum (FNPE): Sclerotinia sclerotiorum (FNPE): data on witlooves collected on Feb. 2009data on witlooves collected on Feb. 2009- 17- 17 accessions to accessions to Thielaviopsis basicola (2Thielaviopsis basicola (2ndnd test test about to be effective in summer 09)about to be effective in summer 09)- 22 accessions - 22 accessions to to Alternaria cichoriiAlternaria cichorii (data sent to (data sent to database manager)database manager)

What will be done in 2009 :What will be done in 2009 :

- data collected in Feb. 09 (- data collected in Feb. 09 (S. ScleritiorumS. Scleritiorum) to be ) to be provided to the chicory database managerprovided to the chicory database manager- evaluation of disease resistance : 72 accessions - evaluation of disease resistance : 72 accessions to T. Basicola and 20 accessions to A. cichoriito T. Basicola and 20 accessions to A. cichorii

Page 12: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 200921/04/2321/04/23

Evaluation for P4 Evaluation for P4 ((Palacky)Palacky) What has been done :What has been done :

- Selection of 30 lettuce accessions for - Selection of 30 lettuce accessions for characterization.characterization.- In 2008, evaluation of 30 lettuce accessions for - In 2008, evaluation of 30 lettuce accessions for response to 3 response to 3 Bremia lactucae Bremia lactucae isolates ; 2008 data isolates ; 2008 data sent to the WP3 leadersent to the WP3 leader

What will be done in 2009 :What will be done in 2009 :- 2008 data to be formatted and sent to database - 2008 data to be formatted and sent to database managermanager- In 2009, evaluation of the lettuce accessions for - In 2009, evaluation of the lettuce accessions for response to 9 new isolates of response to 9 new isolates of Bremia lactucaeBremia lactucae- In 2010, depending on the exact 2009 B.l. Races - In 2010, depending on the exact 2009 B.l. Races tested, 8 races will be testedtested, 8 races will be tested

Page 13: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 200921/04/2321/04/23

Evaluation for P5 (Nordgen)Evaluation for P5 (Nordgen)

What has been done : What has been done : - - RucolaRucola (Eruca) : 20 accessions collected and (Eruca) : 20 accessions collected and submitted in 2008 to chemical evaluations (nitrate, submitted in 2008 to chemical evaluations (nitrate, oxalic acid) ; results sent to database manager.oxalic acid) ; results sent to database manager.- - SpinachSpinach : 51 accessions tested in 2008 (60 : 51 accessions tested in 2008 (60 scheduled) to chemical evaluations with data sent scheduled) to chemical evaluations with data sent to database manager.to database manager.

What will be done in 2009 : What will be done in 2009 : Chemical evaluations Chemical evaluations of 16 orache (of 16 orache (Atriplex hortensisAtriplex hortensis) : spinach or minor ) : spinach or minor crops ?crops ?

Page 14: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 200921/04/2321/04/23

Evaluation for P7 (Evaluation for P7 (Arche Noah)Arche Noah)

What has been done :What has been done :

- Evaluation on winter hardiness made - Evaluation on winter hardiness made on 6 lettuce accessions planted in on 6 lettuce accessions planted in October 08. October 08.

- Data to be provided to the database - Data to be provided to the database managermanager

What will be done in 2009 :What will be done in 2009 :

- Evaluation for quality of 15 other - Evaluation for quality of 15 other lettuce accessionslettuce accessions

Page 15: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 200921/04/2321/04/23

Evaluation for P8 (Evaluation for P8 (HDRAHDRA))

What has been done :What has been done :

Evaluation in 2008 in the field of 16 Evaluation in 2008 in the field of 16 lettuce accessions (instead of 17) lettuce accessions (instead of 17) for for Bremia lactucaeBremia lactucae

What will be done in 2009 : What will be done in 2009 : data for data for 2008 evaluation to be provided to 2008 evaluation to be provided to the database managerthe database manager

Page 16: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 200921/04/2321/04/23

Evaluation for P9 Evaluation for P9 ((University of Bologna)University of Bologna)

What has been done :What has been done :– Evaluation in 2008 of Evaluation in 2008 of 37 Rucola accessions 37 Rucola accessions

(Eruca sativa, Diplotaxis tenuifolia) for (Eruca sativa, Diplotaxis tenuifolia) for glucosinolates content,glucosinolates content,

– Data have been sent to the database managerData have been sent to the database manager

What will be done in 2009 :What will be done in 2009 :- Evaluation for sesquiterpenes in chicory on 97 Evaluation for sesquiterpenes in chicory on 97

accessions (mainly leafy chicories) :accessions (mainly leafy chicories) :- seeds of 75 accessions sent from P2 – GEVES for plant seeds of 75 accessions sent from P2 – GEVES for plant

production during 2009 summerproduction during 2009 summer- seeds of 11 accessions already available in P9 placeseeds of 11 accessions already available in P9 place- production of roots is on going for 11 accessions for production of roots is on going for 11 accessions for

analysis on witlooves during first months of 2010analysis on witlooves during first months of 2010

Page 17: P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed,

P Coquin : 3rd WP coordinators meeting Leafy Vegetables , Warwick, 11 June 200921/04/2321/04/23

Evaluation for P10 (KIS)Evaluation for P10 (KIS)

What has been done :What has been done :Evaluation of 7 accessions for tolerance-resistance Evaluation of 7 accessions for tolerance-resistance to rto root-knotoot-knot nematodes (Meloïdogyne ethiopica). nematodes (Meloïdogyne ethiopica).

What will be done in 2009 :What will be done in 2009 :- - Transmission of data to WP3 coordinator and Transmission of data to WP3 coordinator and database managerdatabase manager

- - Evaluation of 7 other accessions for nematode Evaluation of 7 other accessions for nematode

resistance/tolerance.resistance/tolerance.