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1/3 Copyright © 2018 Micreos Food Safety B.V. All Rights Reserved. This document and the information contained herein remains property of Micreos

B.V. Its content shall not be construed to imply any warranty or guarantee.

Shiga toxin producing Escherichia coli on leafy greens

The nature of cultivation and consumption of fresh produce poses a great food safety challenge with respect to

contamination with Shiga toxin-producing Escherichia coli. Despite the procedure of utilizing antimicrobial washes to

minimize hazard risks, many recent Shiga toxin-producing E. coli O157 outbreaks and illnesses following the consumption of

contaminated produce have occurred.

Up to 99.9 % effectiveness without compromises

The fresh produce industry is looking for effective post-harvest and in-line interventions. PhageGuard E reduces E. coli O157

up to 99.9 % on leafy greens.

PhageGuard E, a two-phage cocktail concentrate, specifically kills E. coli O157. The two phages were isolated and selected

from nature for their specificity and efficacy against a large database of E. coli O157 strains.

Phages are the natural enemy of bacteria. Unlike harsh chemical wash intervention, phages are completely harmless for

fresh produce, personnel, equipment, water treatment installations and consumers. In addition, it kills E. coli O157 without

affecting organoleptic properties such as taste, odor or texture of whole or cut salads.

PhageGuard E reduces E. coli on leafy green vegetables

Green

As the natural enemy of bacteria,

phages specifically kill pathogens

like Shiga toxin producing E. coli and

leave the good ones intact. Organic

phages do not require labeling and

can be applied on organic foods.

Easy

Easy to apply and no rinse

required. PhageGuard E can be

sprayed on whole heads or

packaged cut green salads. It has no effect on taste, color or smell of the final product and as a processing aid it does not require labeling.

Smart

PhageGuard E kills E. coli up to

99.9 %, outperforming chemical

antimicrobial washes that consists

of chlorine and hydrogen peroxide.

14 september, 2018

2/3 Copyright © 2018 Micreos Food Safety B.V. All Rights

B.V. Its content s

All in all, this makes PhageGuard E a smart, green and easy to app

vegetables.

How to use PhageGuard E

PhageGuard E is used as a post-harvest in-

heads after harvest or on cut salads in packaged boxes

For successful phage applications the 3 DDDD’s of success are important:

DDDDistribution – get full coverage of the surface areaphage to encounter the bacteria for an instant kill

DDDDosage – using the right amount of phage to assure adequate kill

DDDDwell or contact time - allow a minimum of 30 minutes before the next surface intervention (or chop (blow up the surface area). On wet sur

Effectiveness of PhageGuard E

To assess the effectiveness of PhageGuard E on leafy vegetables,

different E. coli O157 strains at a level of approximately 1x10

with PhageGuard E dilutions to reach 3x107

negative control. Samples were incubated for 24 hours at 4°C (39

enumerated. Data is an average of three independent experiments with two samples per treatment.

This research demonstrates PhageGuard E

reduction of E. coli O157 on leafy vegetables,

1

2

3

4

5

6

Strain #1

Concentration E. coli Log CFU/cm2

Figure 1. PhageGuard E reduces

hts Reserved. This document and the information contained herein

t shall not be construed to imply any warranty or guarantee.

a smart, green and easy to apply hurdle to further enhance

-line spray or dip application. A PhageGuard E solution

s in packaged boxes by using a conventional spray or electrostatic spray

’s of success are important:

full coverage of the surface area by spray-tumble or dip action and keep the surface wet

for an instant kill

the right amount of phage to assure adequate kill

allow a minimum of 30 minutes before the next surface intervention (

(blow up the surface area). On wet surface are phage will remain effective for

To assess the effectiveness of PhageGuard E on leafy vegetables, romaine lettuce samples were

O157 strains at a level of approximately 1x105 CFU/cm². Following contamination,

PFU/cm2 or 3x108 PFU/cm2, or alternatively with tap water (Control) to serve as a

ere incubated for 24 hours at 4°C (39°F) before the E. coli O157 cells were ret


PhageGuard E is equally effective on all four E. coli O157 strains. More phages result in a higher

O157 on leafy vegetables, showing reductions up to 3-log or 99.9%.

Strain #2 Strain #3

E. coli O157 strain

Control 3x10E7 PFU/cm2 3x10E8 PFU/cm2

Figure 1. PhageGuard E reduces E. coli O157 on Romaine lettuce

3x107 PFU/cm

2 3x10

8 PFU/cm

rein remains property of Micreos

e food safety of leafy green

solution can be applied on whole

or electrostatic spray or by dipping.

and keep the surface wet to allow

allow a minimum of 30 minutes before the next surface intervention (inactivating the phage)

remain effective for up to 24 hours.

omaine lettuce samples were contaminated with

/cm². Following contamination, the samples were treated

, or alternatively with tap water (Control) to serve as a

O157 cells were retrieved and


More phages result in a higher

Strain #4

3x10E8 PFU/cm2PFU/cm2

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B.V. Its content s

USDA & FDA approved

PhageGuard E is USDA and FDA approved. FSIS has completed its evaluation and has no objection to the use of the

O157 bacteriophage preparation as an antimicrobial spray, mist, or wash application (or a mix of these application methods)

on beef to control E. coli O157. No labeling statement is required when used under the accepted conditions of use.

approval for the use PhageGuard E on vegetables is pending.

PhageGuard E is a natural and effective antimicrobial against O157 up to 3-log.

PhageGuard E kills E. coli up to 99.9 %, outperforming chemical antimicrobial washes hydrogen peroxide.

For more information regarding this application data sheet

Micreos Food Safety B.V.Micreos Food Safety B.V.Micreos Food Safety B.V.Micreos Food Safety B.V.

Nieuwe Kanaal 7P • 6709 PA Wageningen

Conclusions

hts Reserved. This document and the information contained herein

t shall not be construed to imply any warranty or guarantee.

PhageGuard E is USDA and FDA approved. FSIS has completed its evaluation and has no objection to the use of the


O157. No labeling statement is required when used under the accepted conditions of use.

approval for the use PhageGuard E on vegetables is pending.

PhageGuard E is a natural and effective antimicrobial against E. coli O157 on leafy vegetables

PhageGuard E kills E. coli up to 99.9 %, outperforming chemical antimicrobial washes

this application data sheet please use the following contact information.

• The Netherlands • Tel: +31 (0)888 007 151 • sales

rein remains property of Micreos

PhageGuard E is USDA and FDA approved. FSIS has completed its evaluation and has no objection to the use of the E. coli


O157. No labeling statement is required when used under the accepted conditions of use. The

leafy vegetables, reducing the E. coli

PhageGuard E kills E. coli up to 99.9 %, outperforming chemical antimicrobial washes consisting of chlorine and

contact information.

[email protected]

www.PhageGuard.com

phageguard e reduces e. coli on leafy green vegetables › ... › 09 ›...

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