updated january 2018 - vetbloomblog.vetbloom.com/wp...braf-mutation-detection-kit.pdfmutation...
TRANSCRIPT
CADETSM BRAF Mutation Detection Assay Updated January 2018
Transitionalcellcarcinomaisthemostcommoncanceroftheurinarytractinthedog.Themostcommonmethodsofdiagnosisareurinarycytology,suctionbiopsyofthebladder,andtissuebiopsyviacystoscopyorsurgery.Allofthesemethodsdetectthecancerlateinthecourseofdisease.TheCADETSMBRAFMutationDetectionAssayisaurine-basedDNAtestforearlydetectionofcaninetransitionalcellcarcinoma.TheBRAFmutationisfoundin85%ofcaninetransitionalcellcarcinomacasesandhasnotbeendetectedintheurineofdogswithothercancersnorindogswithnon-neoplasticinflammatorybladderdiseaseornonmalignantbladdermasses.Thetesthasbeenshowntodetecttransitionalcellcarcinomaindogupto4monthspriortodevelopmentofclinicalsymptoms.TheBRAFMutationDetectionAssayisnotaffectedbyhematuriaorbacteriuria.Dogsthattestpositiveforthemutationcanbemonitoredduringtreatmenttodetecttheextentofresidualdisease.Eachkitcontainseverythingneededtocollectandsubmitthesample.Thereisasmallcardboardboxandawhiteenvelopeforeachkit.
Preparetheurinecollectionpot
1. Removecollectionpotfromtheclearplasticbaganddiscardthebag.2. Thereisasmallvolumeofclearliquidpreservativeinthepot.DO
NOTDISCARDtheliquid.3. Labelthecollectionpotwiththefirstandlastnameofthedogin
CAPITALLETTERSSamplecollection
1. Collecturinebyfreecatchorcatheterintoaseparatecleancontainer.
2. 40-60mlofurineisrequiredforeachtest3. Ifthevolumecannotbecollectedatonce,itisoktocollectsmallervolumesover2-3days.
PackagingandShipping1. Screwthetopontightlyandplacecollectionpotinsidethesuppliedbagwithabsorbentpad.2. Packagebaginsidesmallcardboardboxprovided.PlaceboxinsidesuppliedFedExbag.3. CompletegreenSampleSubmissionForm.4. ShipviaFedEx.NOTE–cannotbeshippedusingFedExdropbox5. SendanemailtoCADETINBOUND@sentinelbiomedical.comwithcollectionpotnumber
(Dx_______________)andFedExtrackingnumberinthesubjectheader.OptionalStudyParticipation
1. ThebottomhalfofthegreensubmissionformisaconsentformandquestionnaireforanoptionalstudyinvestigatinggeneticandenvironmentalfactorsassociatedwithcanineTCC.
Testresultsareemailedwithin2-3businessdaysofreceiptofthesampleatSentinelBiomedical.
2
Resources:
1. CADETSMBRAFMutationDetectionAssayBrochurefromSentinelBiomedical.2. MochizukiH,ShapiroSG,BreenM.DetectionofBRAFMutationinUrineDNAasaMolecular
DiagnosticforCanineUrothelialandProstaticCarcinoma.PLoSOne.2015;10(12):e0144170.doi:10.1371/journal.pone.0144170.PubMedPMID:26649430.
3. DeckerB,ParkerHG,DhawanD,KwonEM,KarlinsE,DavisBW,etal.HomologousMutationtoHumanBRAFV600EIsCommoninNaturallyOccurringCanineBladderCancer--EvidenceforaRelevantModelSystemandUrine-BasedDiagnosticTest.MolCancerRes.2015;13(6):993-1002.doi:10.1158/1541-7786.MCR-14-0689.PubMedPMID:25767210;PubMedCentralPMCID:PMC4470794.