update on rotavirus and update on rotavirus and other ... · other viral causes of acute...
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Update on Rotavirus and Other Viral Causes of Acute Gastroenteritis
Pete Shult, PhD.Director, Communicable Disease Division
WISCONSIN STATE LABORATORY OF HYGIENEWISCONSIN STATE LABORATORY OF HYGIENE 1
,and Emergency Response
Erik Reisdorf, MPH, M (ASCP)CM
Team Leader, Virology Laboratory
WCLN AudioconferenceJanuary 26, 2011
Update on Rotavirus and Other Viral Causes of Acute Gastroenteritis
-Objectives-
• Updated information about norovirus and rotavirus
• Public health impact of rotavirus i ti
Source: www.highlighthealth.comSource: www.highlighthealth.com
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vaccination program• Current epidemiological trends • Importance of public health
surveillance systems
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Global Impact of Gastroenteritis (Diarrheal Diseases)
www.who.int/vaccine_research/diseases/diarrhoeal/en/print.html
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United States Impact of Gastroenteritis
• Estimates of foodborne illness in the U.S.http://www.cdc.gov/foodborneburden/index.html– Each year:
• 48million people (1 in 6) get sick
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p p ( ) g• 128,000 hospitalized• 3000 die
– Estimates can be compounded by community spread
• Only the common cold reported more often than GE of all cases
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FOODBORNE GASTROENTERITISwww.cdc.gov/ncidod/dbmd/diseaseinfo/foodborneinfections_g.htm
Bacterial Agents• Campylobacter spp.• Salmonella spp.• E. coli STEC, ETEC, Other• Shigella spp.• S. aureus• C. perfringens
Viral Agents• Norovirus • Hepatitis A• Rotavirus• Sapovirus• Astrovirus
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C. perfringens• C. botulinum• L. monocytogenes• V. cholerae• V. parahemolyticus• V. vulnificus• Vibrio spp.• B. cereus• Y.enterocolitica• Strep spp., Grp A• Brucella spp.
• Astrovirus• Other ???
Protozoan Agents• Giardia intestinalis• Cryptosporidium parvum• Cyclospora cayatenensis• Toxoplasma gondii• Trichinella spp
Noroviruses 2011http://www.cdc.gov/ncidod/dvrd/revb/gastro/norovirus.htmNew Engl J Med 2009;361:1776-85
A nasty bug and getting nastier– New pandemic strain
identified– Immunity strain-specific
and non-enduring
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g– Highly contagious;
environmentally stable– Food-borne and
community-acquired– Targets restaurants,
schools, chronic care facilities, and cruise ships
– Critical need for personal hygiene!
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NorovirusVirology
• Family Caliciviridae----ssRNA, non-enveloped, 26-34nm viruses
• 4 genera:– Lagovirus– Vesivirus– Sapovirus
Norovirus
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– Norovirus• 5 genogroups
– GI, GII, GIV---humans– GIII---pigs and cows– GV---mice
• ≥ 25 genotypes (genetic clusters)• Ongoing emergence (drift) resulting
in strains among genotypes• GII.4 strains currently predominate
worldwide
NorovirusAntigenic Diversity
GIIGII--3 Toro3 Toro
GIIGII--14 M714 M7
BlakemoreBlakemore
GIGI--7 Winchester7 Winchester
GIGI--8 Boxer8 Boxer
GIGI--3b 3b StavangerStavanger
GIGI--3 DSV3 DSV
GIGI--6 Hesse6 Hesse
318/S05/95318/S05/95GIGI--5 Musgrove5 Musgrove
MaltaMaltaGIGI--4 Chiba4 Chiba
WhiteRoseWhiteRoseGIGI--2 Southampton2 Southampton
KY89KY89GIGI--1 Norwalk1 Norwalk
LordsdaleLordsdaleGIIGII--4 Bristol4 Bristol
GIIGII--17 CSE17 CSE
Kashiwa47Kashiwa47GIIGII--13 Fay13 Fay
GIIGII--10 Erfurt10 Erfurt290/White290/White
GIIGII--5 Hillingdon5 Hillingdon
GIIGII--2 Melksham2 MelkshamSnow MountainSnow Mountain
GIIGII--16 Tiffin16 TiffinGIIGII--12 Wortley12 Wortley314/S19/94314/S19/94GIIGII--1 Hawaii1 HawaiiGirlingtonGirlington
GI: humanGI: humanGII: humanGII: human
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80.10.1
GIIGII--8 8 AmsterdamAmsterdam
GIIGII--9 VABeach9 VABeachGwyneddGwynedd
GIIGII--7 Leeds7 Leeds274/S07/93274/S07/93
GIIGII--6 Seacroft6 Seacroft
GIIGII--18 SWQ18 SWQGIIGII--19 SWQ19 SWQLimburgLimburg
GIIGII--11 SW911 SW9
GIVGIV--1 Alphatron1 Alphatron
Fort LauderdaleFort Lauderdale
GV MNVGV MNV--11
GIIIGIII--1 Jena1 Jena
GIIIGIII--1 CH1261 CH126
NewburyNewbury
Vinje et al. (2009)Vinje et al. (2009)
NorovirusClinical Features
• Incubation period----10-51hr• Duration of illness---24-72 hours• Clinical effects across the age spectrum
– Acute onset of nausea and cramping– Vomiting (more prevalent among children) and
d t di h ( l t
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moderate diarrhea (more prevalent among adults) are hallmarks
– Low-grade fever in up to 50% of cases– Variable constitutional symptoms (e.g., chills,
malaise, headache, myalgia)• Dehydration is most common complication
– May require i.v. replacement fluids• Most patients recover without incident;
fatalities rare
NorovirusPathogenesis and Immunity
• Exact mechanism of diarrhea and vomiting unknown• Observation: asymptomatic infection may occur in
30% of infections– Susceptibility to infection and illness severity
genetically determinedLi k t ABH hi t bl d ti
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• Link to ABH histo-blood group antigens– Role for asymptomatics in transmission?
• Recent GII.4 strains cause more severe disease – More intense symptoms, more fever, longer illness
duration (3-4d); greater transmissability• Immunity strain-specific, but short-lived (months?)
– Re-infection common
NorovirusEpidemiology(I)
Reservoir• Humans are only known reservoir for human infectionModes of Transmission• High levels of virus found in stool and vomit
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High levels of virus found in stool and vomit• Transmission via:
– Food – Water– Direct person-to-person contact
• 2° and 3° cases following point-source outbreak– Contact with contaminated object or surface– Airborne via aerosolization of vomitus
NorovirusEpidemiology(II)
Characteristics That Facilitate Spread:• Low infectious dose (<10-100 viral particles)• Prolonged (up to 4w or more) viral shedding even
in asymptomatic (>90%)– Increased risk from infected food handlers
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– Viral shedding vs. infectivity• Environmental stability and persistence
• Survives:–up to10 ppm chlorine–freezing–up to 60°C–use of many routine disinfectants
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NorovirusTransmissibility
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MMWR (2007) MMWR (2007) NorovirusNorovirus Outbreak Associated with Ill FoodOutbreak Associated with Ill Food--Service Workers Service Workers ------ Michigan, JanuaryMichigan, January----February 2006 56(46). February 2006 56(46).
Outbreaks of Acute GastroenteritisSettings in the U.S.- 2006
Setting Number of Outbreaks
Cruise ships 37
Long-term care facilities 37
Restaurants 13
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Restaurants 13
Hospitals 7
Colleges 3
Parties 3
Other:Schools,daycare,etc. 26
Total 126
NorovirusEpidemiology (III) - Seasonality
FBO by Month (1998-2007)
14161820
aks
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02468
1012
Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec
No
. ou
tbre
a
Bacterial Viral
Source: WI Dept. of Public Health
NorovirusPrevention and Control
Prospects for a vaccine• The clinical and public health motivation is
present• Significant obstacles
– Is morbidity /mortality severe enough?
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Is morbidity /mortality severe enough?– Lack of in vitro propagation methods– Lack of long-term protective immunity
• A local infection– Antigenic diversity:
• multiple genotypes, strains• “influenza-like” drift• Pandemic-like global spread of new strains
NorovirusPrevention and Control
Recommended Measures• Practice good hand hygiene• Aggressive disinfection of contaminated
surfaces
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surfaces• Do not return to work or school until 24-
72h after symptoms resolve– Particularly important with food handlers
• Aggressive measures for outbreak control in healthcare and LTCFs
Rotaviruswww.cdc.gov/rotavirus/index.html
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ROTAVIRUSPublic Health Importance
• Most important cause of severe, dehydrating GE in children <5y in all socioeconomic groups in all regions of the world
• Responsible for ~ 6% of all mortality in children < 5y
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– Mortality predominately in developing world
• > 500,000 deaths annually; >2,000,000 hospitalized
• Malnutrition, less access to Rx, synergy with other pathogens
• However, the story is changing
Mortality Rate per 100,000 Child Deaths due to Rotavirus Disease
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The boundaries and names shown and the designations used on this map do not imply the expression of any opinion whatsoever on the part of the World Health Organization concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. Dotted lines on maps represent approximate border lines for which there may not yet be full agreement. WHO 2009. All rights reserved100 to 500 deaths per 100,000
50 to 100 deaths per 100,000
10 to 50 deaths per 100,000
< 10 deaths per 100,000
Source: WHO/IVB database, 193 WHO Member States. Data as of July 2009Date of slide: September 2009
RotavirusVirology
• Family: Reoviridae– dsRNA, 11 segments, non-enveloped
• 7 serogroups A G; only A B C infect
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• 7 serogroups A-G; only A, B, C infect humans
• Many serotypes within serogroup A– 4 responsible for 90% of pediatric cases
worldwide. However…– Serotype prevalence varies geographically– Reassortment and antigenic drift occur
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RotavirusClinical Features
• Incubation period----24-72 h• Duration of illness---- 3 to 8d• Most severe illness in infants 6m – 2yrs
F iti di h
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– Fever, vomiting, diarrhea– Dehydration with severe electrolyte
abnormalities
• Exacerbating factors: malnutrition, immunodeficiency & poor sanitation
• Decrease in illness severity with age22
RotavirusPathogenesis and Immunity
• Mechanisms of diarrhea and vomiting complex and incompletely understood
• As many as 50% infections subclinical
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• Complex immune response– Innate, cellular, and humoral mechanisms
– Re-infections common throughout life; succeeding illnesses milder
• This is the case even if initial infections asymptomatic
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RotavirusEpidemiology
• Human reservoir• Transmission: person-to-person
– fecal - oral; very rarely waterborne, foodborne
– respiratory?
Ch i i h f ili d
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• Characteristics that facilitate spread– Virus shed in very large amounts; prolonged shedding
– Small infectious dose
– Environmental stability
• Seasonality (“back in the day”)– Marked Winter-Spring peak in temperate climates
•24
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ROTAVIRUSPrevention and control
• Vaccine represents the most promising public health control measure– Natural infection provides protection against
disease
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disease
– Treatments not readily available
– Transmission unaffected by improvements in sanitation and hygiene
– Cost effective
RotavirusVaccines• RotaTeq®
– Pentavalent vaccine containing G1, G2, G3, G4, P[8]
– Licensed by FDA in 2006
– ACIP recommendations in 2006
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ACIP recommendations in 2006
• Rotarix®– Monovalent contains genotype
G1P[8]
– Relies on heterotypic immunity
– Licensed by FDA in 2008
– Widely used in Brazil
RotavirusVaccines
• Uptake nationally: >70% in 6 of 8 states (MMWR, 2010).
• Uptake in WI: 80% had
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received at least one dose of vaccine (MMWR, 2010).
FIGURE. Percentage of rotavirus tests with positive results, by surveillance week --- participating laboratories, National Respiratory and Enteric Virus Surveillance System (NREVSS),
United States, July 2000--June 2009*
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MMWR, Oct 23, 2009/ 58(41);1146MMWR, Oct 23, 2009/ 58(41);1146
Weekly rotavirus admissions (ICD9=008.61) to Wisconsin hospitals, Jul 1992 - Jun 2008
60
70
80
90
ns
2500
3000
3500
ICD9 008.61 introduced 1992
Rotashield Sep 98 - Jul 99
RotaTeqFeb 06 -
Early Returns in the Wisconsin are Promising As Well (I)
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0
10
20
30
40
50
60
Jul-92
Jan-93
Jul-93
Jan-94
Jul-94
Jan-95
Jul-95
Jan-96
Jul-96
Jan-97
Jul-97
Jan-98
Jul-98
Jan-99
Jul-99
Jan-00
Jul-00
Jan-01
Jul-01
Jan-02
Jul-02
Jan-03
Jul-03
Jan-04
Jul-04
Jan-05
Jul-05
Jan-06
Jul-06
Jan-07
Jul-07
Jan-08
Jul-08
Week of admission
Nu
mb
er o
f h
osp
ital
iza
tio
n
0
500
1000
1500
2000
Early Returns in the Wisconsin are Promising As Well (II)
Number of Specimens Tested and Positive for Rotaby Wisconsin Antigen Detection Sites
200
250
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30
0
50
100
150
7/3/
04
9/25
/04
12/1
8/0
43/
12/0
56/
4/0
58/
27/0
511
/19
/05
2/11
/06
5/6/
06
7/29
/06
10/2
1/0
61/
13/0
74/
7/0
76/
30/0
79/
22/0
712
/15
/07
3/8/
08
5/31
/08
8/23
/08
11/1
5/0
82/
7/0
95/
2/0
97/
25/0
910
/17
/09
1/9/
10
Nu
mb
er
No. Positive for Rota No. Rota Tested
6
Countries Using Rotavirus Vaccine in National Immunization Schedule, 2008
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31Source: WHO/IVB database, 193 WHO Member States. Data as of July 2009. 2009 data is provisionalDate of slide: September 2009
The boundaries and names shown and the designations used on this map do not imply the expression of any opinion whatsoever on the part of the World Health Organization concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. Dotted lines on maps represent approximate border lines for which there may not yet be full agreement. © WHO 2009. All rights reserved
No (170 countries or 88% of countries)
Yes (17 countries or 9% of countries)
Yes (Part of the country) (2 countries or 1% of countries)
Introduction in 2009 (6 countries or 3% countries)
Yes (Risk groups) (1 country or 0.5% of countries)
In 2008,Peru and South Africa had introduced in parts of the country and Colombia for risk groups
Rotavirus VaccinesProspects for Global Success
Recent successes: 2006-07 – Mexico – Africa
Anticipated challenges– How reproducible are results in other countries
St d hi t i t
N Engl J Med N Engl J Med 362362;;4 14 1//2828//1010
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– Storage and shipment requirements– Narrow window for vaccine administration*
• 1st dose between 6-15 weeks and 3rd dose no later than 32 weeks
– Cost of vaccine in the U.S.– Past experience with oral vaccines in developing countries– Antigenically changeable virus - will immunization drive
evolution?• Need to maintain/expand surveillance
Norovirus vs. RotavirusAn interesting public health comparison
One on the risevs.
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vs.One on the decline
Norovirus:Laboratory methods
• Cell culture• RT-PCRHigh sensitivity, specificity, throughput,
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g y p y g psame day results.
Limitations: Infectious virus, inhibitors
• EIASensitivity =55% compared with RT-PCR
(Moe, 2004).Specificity =83 to 96% (Gray, et al., 2007)
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Norovirus:National Surveillance
• CaliciNet17 states certified March 2010Wisconsin one of 5 SPHL selected Specimens received from 3 Midwest statesSeq ences deposited in national database
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Sequences deposited in national databaseObjectives:Improve surveillance Real-time data exchangeLinking clusters of illnessMonitor for emerging strains
CaliciNet: Geographical representation
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Courtesy of CDC National Calicivirus Laboratory
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Norovirus diversity
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Courtesy of CDC National Calicivirus Laboratory
Norovirus WI Surveillance
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Courtesy of Wisconsin Division of Public Health Bureau of Communicable Disease
Norovirus WI Surveillance
• Wisconsin strain surveillance
6%5%
Norovirus genotypes in WI Sept. 2009-May 2010
Norovirus genotypes in WISept. 2010- Jan. 2011
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6%
35%
5%8%
3%3%
35%
5% GI.3B
GII.12
GI.4
GI.6
GII.3
GII.4_Minerva
GII.4_NewOrleans
4% 6% 2%7%
4%
65%
2% 4% 6% GI.1
GI.4
G1.7
GII.2
GII.4_Minerva
GII.4_NewOrleans
GII.4_Osaka
GII.6
NorovirusEpidemiology
PANDEMICs occur every 2-4 years!
Emergence of novel GII.4Possess different epidemiological profile
Attack rateSource: www.stuffwelike.com/.../2009/11/Pandemic.jpg
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Vomiting & diarrheaDuration of illness
2002 GII.4_Farmington Hills 2006 GII.4_Minerva
WDPH investigated 106 AGE v. 23 in 2005!
2010 GII.4_New OrleansWDPH investigated 37 outbreaks in
December 2010!
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Norovirus Summary
• Genetically diverse• Antigenic drift• GII.4 pandemic potential
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G pa de c pote t a• National and community impact
uncertain• Vaccine faces many challenges• Knowledge base continues to expand
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RotavirusLaboratory methods
• EIA* Sensitivity >90%– BioRad Pathfinder, Vidas, Rotaclone,
etc…
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Limitations:NPV high
PPV low
GrpA specific
• RT-PCR• Sequencing
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Rotavirus Molecular Epidemiology
• US Rotavirus strain surveillance data: 2005-2008 (Hull et al., 2011)
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Year Genotypes
2005-2006 G1 predominate
2006-2007 G1 predominate
2007-2008 G1 & G3 predominate, G2, G9
Rotavirus vaccine: Impact on strain diversity and age distribution
• Key findings (Hull J et al., 2011)– Reports from Brazil & Australia described
changes in genotype prevalence post-vaccine
– US: G3 emerged as predominate rotavirus
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genotype in some sentinel sites (2007-2008)
– G3 predominated in states primarily using RotaTeq® (natural fluctuation vs. vaccine driven)
– Mean age of rotavirus patients increased from • 13 to17.8 months in 2007-2008.
Is diversity natural fluctuation or vaccine driven??
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Rotavirus surveillance--Wisconsin
• Collaboration between CDC/WSLH/WI Clinical Laboratories
• Aim is to assist CDC with national
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strain surveillanceMonitor for emerging strains
Change in genotype prevalence
Ensure vaccines are effective
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Rotavirus specimen submission
• Please send ALLrotavirus positive specimens to WSLH
• NO cost for shipping if using Dunham
Patient Information Submitter InformationName (Last, First): (Your Institution’s Agency Number If Known)
Address: (Your Institution’s Name)
City: State: Zip:
(Your Institution’s Address)
Age or Date of Birth: (City, State, Zip Code)
Gender: M F (Telephone Number)
Patient Telephone Number: Health Care Provider Full Name:
Your Specimen ID Number (optional):
WSLH Use Only: VI ROTA CDC / Bill To: Account # 74201
Date Collected: Specimen Type:
[ ] Raw Stool
SAMPLE: Rotavirus Surveillance Requisition Form [rev. 11/2010]
WISCONSIN STATE LABORATORY OF HYGIENEWISCONSIN STATE LABORATORY OF HYGIENE
using Dunham• Specimens can be sent
weekly (refrigerated)• Sent with Flu
surveillance specimens• Raw stool or VTM
acceptable46
[ ] Raw Stool
[ ] Other (specify):_________________________Onset Date:
Vaccination History:A. Was the patient vaccinated for rotavirus? [ ] Yes [ ] No [ ] UnknownB. What date was the patient vaccinated: ________________(MM/DD/YR) [ ] UnknownC. Which vaccine was used? [ ] RotaTeq® [ ] Rotarix® [ ] Unknown
Your Test Results
[ ] Positive Rotavirus[ ] Negative Rotavirus
[ ] Other (specify):_________________________
Please mark the test used: Antigen Detection[ ] BioMerieux Vidas/Mini [ ] ImmunoCard STAT![ ] Xpect Rotavirus [ ] Premier Rotaclone
[ ] Sure Vue Rotavirus [ ] Pathfinder Rotavirus EIA[ ] Murex Rotavirus[ ] SAS Rota [ ] Other (specify): _______________________
WISCONSIN STATE LABORATORY OF HYGIENE USE ONLY
WSLH Test Code: 3222
Rotavirus Summary
• Diverse genome• Vaccine impact on morbidity & prevalence• Vaccine impact on strain evolution
( t G&P i l d d i i )
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(genotypes G&P included in vaccine)– Brazil and US noted a shift in prevalence of
other genotypes following vaccination (Gentsch et al., 2009). Natural fluctuation or vaccine driven?
– Importance of monitoring viral genotype prevalence.
Future Concerns
• Monitoring for emerging strains of norovirusand rotavirus.
• No national surveillance system for norovirus– Difficult to assess public health impact
E f i
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• Emergence of sapovirus– Studies have concluded that sapovirus infections
are increasing in Europe (Svraka et al., 2010).
• What is the significance of other viral etiologies? – Adenovirus 40/41, astrovirus, sapovirus, unknown
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Diagnostic gap
• What role do viruses other than norovirus play in outbreaks and AGE morbidity in the US? Etiologies unknown in 12-41% of outbreaks (Lyman et al.,
2009 & Finkbeiner et al., 2009).
d
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• WI NFBO investigation data:
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Year NV Unknown % Other
2006 61 11 15.3
2007 41 12 22.6
2008 90 5 5.3
2009 102 21 17.1 Sapovirus
2010 110 27 19.7Courtesy of John Archer WI Division of Public Health (2010)
References
Atmar, R et al. (2008) Norwalk virus shedding after experimental human infection. Emerg Infect Dis. Oct;14(10):1553-7.
Bull et al. (2010) Rapid evolution of pandemic noroviruses of the GII.4 lineage PLOSpathogens 6(3). Cunliffe, N et al. (2010) Healthcare-associated viral GE among children in a large pediatric hospital, UK EID 16(1): 55-62.
Curns et al. (2010) Reduction in acute GE hospitalizations… JID 201: 1617-1624. Finkbeiner, S et al. (2009) ID of a novel astrovirus (vA1) associated with an outbreak of acute GE J Virology 10836-10839. Gentsch et al. (2009) Impact of rotavirus vaccination: The importance of monitoring strains Future Microbiology 4(10):
1231-1234. Gentsch J et al. (2009) G & P types of circulating rotavirus strains in the US during 1996-2005: Nine years of prevaccine
data JID 200(Sup1): S99-S105 Glass et al. (2009) Norovirus gastroenteritis NEJM 361(18): 1776-1785. Glass, RI, Bresee, J, Jiang, B, et al. Gastroenteritis viruses: an overview. Novartis Found Symp 2001; 238:5. Gray et al. (2007) European Multicenter Evaluation of Commercial Enzyme Immunoassays for Detecting Norovirus Antigen in
Fecal Samples Clin Vaccine Immunol. October; 14(10): 1349–1355
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p ( ) Hull J et al., (2011) US rotavirus strain surveillance from 2005-2008 Pediatric Infectious Disease 30(1). Lyman, W et al. (2009) Prospective study of etiological agents of acute gastroenteritis outbreaks in child care centers J
Pediatrics 154: 253-257. KSU (2010) Norovirus fact sheet Available at:
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