team e presents: micro fluidic paper-based assay device
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Alan Dorsinville, Reading High School Natalie Gibbs, Reading High School. Team E Presents: Micro Fluidic Paper-Based Assay Device. Outline. Introduction The Problem and our solution Background Information The purpose of μ PAD’s Materials Procedure Science Concepts Results - PowerPoint PPT PresentationTRANSCRIPT
Alan Dorsinville, Reading High SchoolNatalie Gibbs, Reading High School
Introduction The Problem and our solution Background Information The purpose of μPAD’s
Materials Procedure Science Concepts Results Team F(Floral Experiment) Conclusion/Discussion Further Research Acknowledgements
The cost of health care is an issue in America
Testing requires Time Money Insurance Technical Experience Etc
All of which is inconvenientDeveloping Countries
Micro fluidic paper-based assay devices (μPAD) A paper diagnostic test
Paper-based devices are Inexpensive Quick Easy to use Require a small volume of liquid Lack the use of advanced equipment Effective and accurate
Your body has many substances including proteins and glucose
Glucose provides energy for your body an all of your movements
Glucose Concentration
Disease
0-0.8mM Normal
Above 0.8mM Impaired kidney and/or diabetes
Proteins are important for growth, tissue repair, and many other bodily functions
Our purpose is to show we can quantify diagnostic results using a cheap paper device Our chips are designed to detect glucose
and protein in our substances
Intermolecular forces
Capillary action Allows us to direct small amounts of
liquid to testing wells
CleWinChromatography paper( Whatman)PrinterScales, beakers, pipettesVarious chemicals Infrared GunHot PlateScannerAdobe Photoshop
Part One: PlanningCleWin is a computer program made
to design our chips
Step 1: Printing The pattern is outlined with wax when
printedStep 2: Place the chips on a hot plate
at 150°C Allows wax to seep through
This project requires making both a protein and glucose reagent A chemical reagent is a substance used
in a chemical reaction to detect, measure, examine, or produce other substances
Buffer (pH=6.0) 0.2 M NaH2PO4 0.2 M Na2HPO4
0.3 M Trehalose0.6 M KI30 units/mL HRP120 units/mL GO
Glucose + Glucose oxidase Gluconic acid + Hydrogen
peroxide (H2O2)
H2O2 H2O + ½ O2
I- ½ I2
HR Peroxidase
Brown color
Part 1: 0.25 M Citric acid (pH 1.8 buffer) 184 μL H2O, 16 μL EtOH
Part 2: 9 mM TBPB 10 μL H2O, 190 μL EtOH
Protein Mechanism TBPB + protein = Blue color
Apply 0.2 μL of reagents using a micropipette. Must wait ten minutes
Part Five: Test One
We made a new design for our second chip on CleWin, printed them, and reapplied the chemical reagents, etc.
The chips are a urine analysis test so we made an artificial urine sample
We made several concentrations glucose and proteins to test
Sol'n 1 2 3 4 5 6 7 8 9 10 11Glucose 25 18.75 12.5 9.375 6.25 5 3.75 2.5 1.25 0.63 0BSA 25 20.83 16.67 12.5 8.33 4.17 3.125 2.08 0.833 0.42 0Urine 0 10.42 20.83 28.13 35.42 40.83 43.13 45.42 47.92 48.96 50
We performed eight tests for each of the eleven different concentrations of glucose and protein
After 30 minutes, the chips were scanned into the computer
Team F’s research is focused on the relationship between pollinators and nectar
We made adjustments to our second chip to create a more effective test
We were not able to quantify, but still proved the chips had the potential to quantify different detectable substances
We were able to rank the glucose concentration of Team F’s nectar solutions
The μPAD’s serve the same purpose as other urine tests Scientists are trying to find ways of
detecting more diseases with the paper-based analytical device
We would like to thank:Dr. Scott PhillipsSeeCos FacultyChris DalyMs. Jody MarkleyMr. Derek JamesMs. Jean Marie DonnellyUBMS Staff