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Alan Dorsinville, Reading High SchoolNatalie Gibbs, Reading High School
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Introduction The Problem and our solution Background Information The purpose of μPAD’s
Materials Procedure Science Concepts Results Team F(Floral Experiment) Conclusion/Discussion Further Research Acknowledgements
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The cost of health care is an issue in America
Testing requires Time Money Insurance Technical Experience Etc
All of which is inconvenientDeveloping Countries
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Micro fluidic paper-based assay devices (μPAD) A paper diagnostic test
Paper-based devices are Inexpensive Quick Easy to use Require a small volume of liquid Lack the use of advanced equipment Effective and accurate
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Your body has many substances including proteins and glucose
Glucose provides energy for your body an all of your movements
Glucose Concentration
Disease
0-0.8mM Normal
Above 0.8mM Impaired kidney and/or diabetes
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Proteins are important for growth, tissue repair, and many other bodily functions
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Our purpose is to show we can quantify diagnostic results using a cheap paper device Our chips are designed to detect glucose
and protein in our substances
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Intermolecular forces
Capillary action Allows us to direct small amounts of
liquid to testing wells
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CleWinChromatography paper( Whatman)PrinterScales, beakers, pipettesVarious chemicals Infrared GunHot PlateScannerAdobe Photoshop
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Part One: PlanningCleWin is a computer program made
to design our chips
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Step 1: Printing The pattern is outlined with wax when
printedStep 2: Place the chips on a hot plate
at 150°C Allows wax to seep through
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This project requires making both a protein and glucose reagent A chemical reagent is a substance used
in a chemical reaction to detect, measure, examine, or produce other substances
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Buffer (pH=6.0) 0.2 M NaH2PO4 0.2 M Na2HPO4
0.3 M Trehalose0.6 M KI30 units/mL HRP120 units/mL GO
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Glucose + Glucose oxidase Gluconic acid + Hydrogen
peroxide (H2O2)
H2O2 H2O + ½ O2
I- ½ I2
HR Peroxidase
Brown color
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Part 1: 0.25 M Citric acid (pH 1.8 buffer) 184 μL H2O, 16 μL EtOH
Part 2: 9 mM TBPB 10 μL H2O, 190 μL EtOH
Protein Mechanism TBPB + protein = Blue color
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Apply 0.2 μL of reagents using a micropipette. Must wait ten minutes
Part Five: Test One
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We made a new design for our second chip on CleWin, printed them, and reapplied the chemical reagents, etc.
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The chips are a urine analysis test so we made an artificial urine sample
We made several concentrations glucose and proteins to test
Sol'n 1 2 3 4 5 6 7 8 9 10 11Glucose 25 18.75 12.5 9.375 6.25 5 3.75 2.5 1.25 0.63 0BSA 25 20.83 16.67 12.5 8.33 4.17 3.125 2.08 0.833 0.42 0Urine 0 10.42 20.83 28.13 35.42 40.83 43.13 45.42 47.92 48.96 50
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We performed eight tests for each of the eleven different concentrations of glucose and protein
After 30 minutes, the chips were scanned into the computer
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Team F’s research is focused on the relationship between pollinators and nectar
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We made adjustments to our second chip to create a more effective test
We were not able to quantify, but still proved the chips had the potential to quantify different detectable substances
We were able to rank the glucose concentration of Team F’s nectar solutions
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The μPAD’s serve the same purpose as other urine tests Scientists are trying to find ways of
detecting more diseases with the paper-based analytical device
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We would like to thank:Dr. Scott PhillipsSeeCos FacultyChris DalyMs. Jody MarkleyMr. Derek JamesMs. Jean Marie DonnellyUBMS Staff
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