Summer!Undergraduate!

Research!

Fellowship2014

Rhode Island NSF EPSCoR’s Summer Undergraduate Research Fellowship (SURF) program helps grow the state’s economy by developing a skilled workforce and training future generations of scientists to conduct cutting edge research.#

The program provides Ocean State students a unique opportunity to engage in full-time, independent research in the laboratory of a faculty mentor. Students earn fellowship awards through a competitive application process and spend 10 weeks on their research projects, which culminate with a presentation of their work at an annual conference.#

The student researchers also take field trips to relevant industries and develop professional skills through topical seminars and career-building workshops such as science ethics and leadership, strategies for graduate school admission, and science communication.#

Since 2007, Rhode Island NSF EPSCoR has supported 175 SURF fellows under the mentorship of 76 different faculty members at our partner institutions.

Opportunities of a lifetime for RI undergrads

Rhode Island NSF EPSCoR SURF 2014!Laura Anderson | Roger Williams University!

Effects of ocean acidification on larval fish!!Mentor: Dr. Andrew Rhyne!Major: Marine Biology!Career path: Undecided!Expected graduation: Spring 2017SURF project goal: We are trying to determine the relationship between ocean acidification/ocean pH and the growth of otoliths (ear stones) in larval fish. We expect to see enlarged otoliths in the fish due to the acidified water. This affects their swimming ability, and therefore their overall fitness. Why SURF?: Not only did I want to gain experience in a laboratory environment, but I wanted to learn about career options I may want to consider in the future.  The SURF experience: So far this summer I have had the opportunity to gain skills both in and out of the laboratory. I have had the chance to raise larval fish, use new microscopes to examine the fish, and I have been able to write to help teach others about what we are learning. The best part of the SURF program has been the team of people I’ve gotten to work with. I think it says a lot that even on our days off we plan things to do together, whether it is going to the beach, going on hikes, or even just playing guitar on our lunch breaks. Memorable research moment: There have been too many to count, but probably the day we had so much work to do that we stayed in the lab until 2:30 in the morning! At that point, everything was funny, so we were having fun even though it was so early. Either that, or the times where something has not gone according to plan. This was not necessarily a bad thing, though, because it forced our team to get creative to solve problems. One memorable moment was when the CO2 line into our part of the lab broke three times in one day! Even though it was frustrating, we bonded over the experience. !

Rhode Island NSF EPSCoR SURF 2014!Nicholas Andreozzi | Rhode Island College!

Do chemicals in the environment affect embryonic development in cartilaginous marine fish?!!Mentor: Dr. Rebeka Merson!Major: Secondary Education & Biology!Career path: Undecided!Expected graduation: Spring 2016SURF project goal: Determine if certain chemicals that are dumped into Narragansett Bay are changing the development of fish (particularly the little skate and the spiny dogfish). Also, determine where associated genes are expressed in the embryos of the little skate. I expect the chemicals to be affecting the organisms in the bay, but I don’t know where the associated genes may be expressed.Why SURF?: The project was very interesting to me because I like marine biology and fish, but also molecular biology. The most valuable part of the summer so far has been the experience of working full time in a lab. This is helping me narrow down what I want to do after my degree.Memorable research moment: The research group and I took a trip to the Environmental Protection Agency to collaborate with Diane Nacci and her colleagues about how to best perform an experiment we were going to do. On this trip, we got a tour of the facility and saw all of the killifish they had there and how they took care of them.

Rhode Island NSF EPSCoR SURF 2014!Shannon Aurigemma | Roger Williams University!

How is protein expression of marine invertebrates affected by projected ocean temperature and pH changes?!!Mentors: Dr. Steven Irvine, Dr. Thomas Meedel!Major: Marine Biology & Environmental Science!Career path: Work with marine organisms, possibly coral reef fishes, and be involved in aquarium design. ! Expected graduation: Spring 2016SURF project goal: Observe the effects of temperature change on the sea squirt species Ciona Intestinalis. The lower temperature demonstrates normal conditions while the higher temperature illustrates the predicted increase in ocean temperature on account of climate change. We will observe the effects on the species by carrying out protein and RNA extractions to see if there are differences in the proteins expressed. We will also observe difference in reproductive success. We expect that there will be differences in the proteins expressed in the two populations, thus showing that this environmental stress of temperature increase will have harmful effects on the species. !Why SURF?: I wanted to learn how to raise marine organisms and work in the lab. I also wanted to have an internship that involved science in the field. This project is teaching me a lot about the difficulties in research. There are so many factors that influence the proteins expressed in any organism and it is difficult to isolate the factors we do want from the ones we do not want. When I found out that I was accepted to the program it was nighttime, but I screamed anyway, waking up my roommate. I still have that same exuberance months later. !Memorable research moment: Setting up the tank at the Graduate School of Oceanography, we were trying to figure out how to work the chiller pump to regulate the temperature of one of the tanks. Dr. Irvine was holding up one of the ends of the tubes connected to the chiller, while the other tube was in one of the tanks filled with water. Suddenly, I realized that the tubing was pointed directly at me. I held my right arm straight out, as if to say stop and my mouth was open wanting to say, “STOP!” but my mouth and brain were not cooperating. Within an instant, the chiller pump started working and water sprayed me in the face. It was actually pretty refreshing because that room is always so warm. We laughed for a little and continued on. !!

Rhode Island NSF EPSCoR SURF 2014!Jennifer Brewster | Bryant University!

Conducting desorption studies of various amines using river sediment (Blackstone River Project)!!Mentor: Dr. Chris Reid!Major: Biology!Career path: Industrial biotechnology research!Expected graduation: Spring 2016SURF project goal: We are trying to find out which antibiotic-resistance genes — or genes inside these bacteria that prevent them from expiring from antibiotics — are present in bacteria in the Blackstone River, and identify what bacteria carry them. I hope to learn more about the methods related to my field of study, along with gaining enough data from this experiment to continue studying the Blackstone River after the fellowship ends.!Why SURF?: I wanted to make a closer connection with my professors and other science majors at Bryant, along with gaining a greater understanding of lab procedures and general experience in the lab with tools, methods, and the like. Through EPSCoR, I’ve been able to learn with my peers in a lab setting about interesting projects related to our interests, along with getting to know them and my professors better.!Memorable research moment: While cleaning our lab for the weekend, we discovered a lump of fungus in one of our broths. We decided to keep him (for now) and named him Collin: Our colony of fuzzy fungus!!!

Rhode Island NSF EPSCoR SURF 2014!Joe Burgess | Rhode Island College!

Heat shock proteins in Geukensia demissa as indicators of climate change and environmental stress on marine life in Narragansett Bay!!

Mentor: Dr. John Williams!Major: Biology!Career path: Research!Expected graduation: 2014SURF project goal: We are trying to use the Atlantic ribbed mussel as an indicator for climate change. When any organism becomes too warm, its proteins become damaged, causing the organism to release heat shock proteins that help get rid of or fix damaged proteins. We are measuring the amount of heat shock proteins the Atlantic ribbed mussel is expressing in the wild. If this project is continued into the future, we believe the mussel will express more heat shock proteins as global warming keeps heating the ocean. I expect that we will come up with a reproducible way to measure levels of heat shock proteins in the wild.Why SURF?: I wanted to be able to continue doing research into the summer. I’ve had a great experience, and I’m learning many valuable skills that can only come from hands-on learning experiences.Memorable research moment: The day we spent on the beautiful Jamestown beach, analyzing mussels, when we were asked by an interested child if we were wizards.!

Rhode Island NSF EPSCoR SURF 2014!Kelli Butler | University of Rhode Island!

What is the potential for evolutionary adaption of marine organisms to climate change?!!Mentors: Dr. Jason Kolbe, Dr. Carol Thornber & Ph.D. student Gordon Ober!Major: Environmental Science and Management!Career path: Coral conservation & restoration!Expected graduation: 2016SURF project goal: Our team is exploring a species' evolutionary ability to adapt to climate change. To investigate this, we are using a mysid shrimp species, Neomysis from Narragansett Bay. !Why SURF?: I have always been concerned with climate change and how species will adapt to these changes. This project is very much in line with my interests. I was also looking to gain experience within a professional lab setting focusing on climate change. This experience has been great! I have learned a great deal about setting experimental parameters and properly executing them. Memorable research moment: There have been many memorable moments, but the most memorable was when our team went out on the bay to catch the species we have been working with this summer. The mysid shrimp are relatively small and we had to separate them from the other items brought up in the trawler. It was exciting to collect the first generation of Neomysis that we would be working with for the project.

Rhode Island NSF EPSCoR SURF 2014!Kyle Carpentier | University of Rhode Island!

Impacts of climate change on harmful macroalgal blooms!!Mentor: Dr. Carol Thornber!Major: Biological Sciences!Career path: Help educate the public about the benefits of certain genetically modified organisms (GMOs) through research or a public relations position.!Expected graduation: December 2015SURF project goal: Our goal is to determine the effects climate change will have on a type of seaweed that grows all around the coastline of Greenwich Bay. There is a severe lack of literature on the algae genus Ulva and its response to a prolonged exposure to decreased salinity paired with an increase in water temperature. The beauty of not having any sort of preconceived notion is that it allows us to maintain a strictly objective outlook on this entire experiment.Why SURF?: Since the beginning of my college experience I knew that I wanted to be involved in biology in some sort of fashion. However, it wasn’t until this past school year that I recognized my passion for research. It is for this reason that I chose to go after the research opportunity that the SURF program provides us, in hopes of confirming that this is indeed what I want to pursue. My experience has been nothing but rewarding. This is not to say that everything has gone exactly as planned or been even close to perfect. However, it is from these mishaps and imperfections that I have found lessons to be learned, which will be extremely useful, not only in practicing research, but in all other areas of my professional and personal life.Memorable research moment: The first time our team went to the Graduate School of Oceanography at URI’s Bay Campus was a surreal experience. It was my first time there. Being surrounded by a beautiful landscape that consists of many significant Rhode Island landmarks, getting my first taste of what it was like to set up an experiment with the possibility of it getting published, all while being around the many sea creature friends in the tanks made for a wonderful SURF summer memory.!

Rhode Island NSF EPSCoR SURF 2014!Samuel Church | Brown University!

The cellular structure of complex colonial animals !!Mentor: Dr. Casey Dunn!Major: Biology!Career path: Research/university teaching!Expected graduation: June 2015SURF project goal: Siphonophores are a kind of hydrozoan, or in common terms, jellyfish. These animals, unlike the “classic” jelly, grow into long, complexly organized colonies that swim freely. The siphonophore colony accomplishes this through budding new cloned individuals, which remain attached and perform specialized functions such as feeding or reproducing. At the location where buds begin, a small patch of stem cells has been reported; these cells will divide and become the cells of the whole colony. My project is to characterize, using very high magnification microscopes, the structures of these cells and their derivatives in mature siphonophore colonies. I expect to find more complexity in the cellular structure than is commonly considered for jellyfish. It is often stated that these are “simple” animals, though the closer we look the more we can fully comprehend their complexity.Why SURF?: The SURF program provides the sort of flexible, yet specific funding that allows research to achieve a higher potential. The funding was very supportive in allowing me to pursue a project that fit with my studies as well as with the Dunn lab. I have the ability to make progress on my own – to direct my own project and to further understanding with regard to open questions. This summer feels like it is going to be one of the more productive and satisfying experiences of my scientific experience to date. Memorable research moment: One week, despite having put in many hours on the microscope, I was not able to image certain cells with enough resolution to provide definitive results. I was pretty disappointed, but then decided to make a final attempt using some slides I had made previously. Right when I was about to call it a day, I found the shot I needed and was able to tie up a lot of loose ends.!!

Rhode Island NSF EPSCoR SURF 2014!Jennifer Cyr | Providence College!

Phylogenetic ties in mysid shrimp species!!Mentor: Dr. Elisabeth Arevalo!Major: Biology!Career path: Researcher/graduate school — infectious disease in wildlife!Expected graduation: Spring 2015SURF project goal: Discover the genetic makeup of several species of mysid shrimp. Since no one has successfully characterized these genomes to date, my lab may be among the first. Currently, different mysid species are defined only by morphological and behavioral attributes. A genetic component to this definition would be extremely helpful in many important toxicology and climate change studies because these shrimp are a model organism used by the Environmental Protection Agency (EPA) for such studies. We expect to come up with a variety of tools to use in order to make the genetic characterizations. This means that in the lab, we generally focus on trial-and-error tests of various genetic markers. I hope to have figured out at least five markers that “work.”!Why SURF?: I have worked with my mentor, Dr. Arevalo, for three years and am familiar with the techniques used in her lab. When I heard she was starting a new project (we used to work with wasps), I was eager to learn something new. This summer has been a great experience. I have thoroughly enjoyed field work and the additional lab experience. The program has allowed me to really get a feel for all branches of lab science – field collection, molecular experiments, and behavioral observation, to name a few.!Memorable research moment: Our lab group took a trip south to collect mysids. We had to wake up very early and collect and count a ton of shrimp, but we also had time to experience the culture and attractions of Florida, Alabama, New Orleans, and Mississippi (most of which I’d never been to).!!

Rhode Island NSF EPSCoR SURF 2014!John Da Lomba | Community College of Rhode Island!

Do stress and diet affect the composition of intestinal mucous in summer flounder?!!Mentors: Dr. Bruno Soffientino & Dr. Marta Gomez-Chiarri!Major: General Studies!Career path: Environmental engineer/biological research!Expected graduation: 2016SURF project goal: If summer flounder fish are given a remote plant protein source rather than a fishmeal protein source will it affect the intestinal mucous lining of the organs? Our research will surely benefit the men and women of aquaculture. It may be possible to feed summer flounder a much more sustainable and cheaper source of protein such as soybean meal and still see the population flourish and grow. I hope to utilize what I have learned and apply it to the world around me. With the knowledge I have gained, I feel much more comfortable being in a laboratory setting among other scientists. I hope to work in a laboratory at a four year institution after transferring from CCRI.!Why SURF?: I wanted to gain hands-on experience, discipline and feedback from some of the best scientists in the state. It is amazing how a program like this could even exist. I am grateful to my mentor, Dr. Soffientino, for proposing the idea that I apply to the SURF program. I thought it would be a great place to begin my endeavors in life, get the opportunity to learn and gain experience in the biology field. Now I know I’ll be well prepared to enter a laboratory setting.Memorable research moment: Devising a plan in tissue staining. The field of Immunohistochemistry is fairly new and techniques in staining slides are always being developed. Limiting or increasing the time that chemical reagents can bind to tissue glycoconjugates can always be modified and always changed. The results of the experiment can always be altered so timing is key. Being at the forefront of the experiment lets me be in control and gives me great responsibility. !!

Rhode Island NSF EPSCoR SURF 2014!Anthony DelPizzo | Providence College!

How can cutting edge genetic methods best be used in conservation biology?!!Mentors: Dr. Jeffrey Markert & Dr. Elisabeth Arevalo!Major: Biology!Career path: Biopharmaceutics/biotech!Expected graduation: Spring 2015SURF project goal: We are exploring the many different species of Americamysis (from Rhode Island and Florida and Mississippi) and looking for phylogenetic relationships between the species. I, personally, am doing the genetics aspect of the project — extracting the DNA from the mysids and trying to see what markers fit with each species in hopes to sequence the genome. We expect to find a few markers that are working, which has been a long process of PCR and electrophoresis. Why SURF?: I wanted to get involved in research and felt as if the SURF program allowed me to do so without sacrificing my time in another job. SURF has been an awesome experience. Working in a lab full time has been great and I am learning a lot more than I expected to, while also enjoying my time. Memorable research moment: Our research group went down to Florida and Mississippi to collect mysids in the Gulf of Mexico and it was a great experience in the field, which I was not used to since I mainly do genetics testing in the lab.

Rhode Island NSF EPSCoR SURF 2014!Kristian Dzilenski | University of Rhode Island!

Causes of long-term declines of coral reef communities!!Mentor: Dr. Graham Forrester!Major: Marine Affairs!Career path: Conservation biologist!Expected graduation: December 2014SURF project goal: We are conducting an investigation into the characteristics of operations of Reef Guardians, British Virgin Islands, a volunteer-based lionfish removal effort. I suspect their efforts will be biased towards popular recreational dive sites that support the tourist industry.Why SURF?: It is a selective program and provides experience in the practical application of research methodology. My project partner and I have spent many hours transcribing and analyzing data. The development portion of the project goals has provided a taste for what it is like to be an actual scientist seeking to answer an inquiry of interest.Memorable research moment: I am looking forward to several weeks in the field, employing SCUBA and AAUS research diver skills under the experienced tutelage of Dr. Forrester.

Rhode Island NSF EPSCoR SURF 2014!Mike Finnerty | Providence College!

The feeding behavior of Opossum shrimp!!Mentor: Dr. Maia Bailey!Major: Biology!Career path: Unknown!Expected graduation: May 2016SURF project goal: We are interested in the overall behavioral patterns of mysid shrimp. This can be defined observing behaviors between species and within species via observational analysis. Furthermore, we are trying to determine their feeding patterns by counting out specific amounts of food, or smaller brine shrimp. We hope to be able to recognize a specific volume of food per mysid shrimp. From this, we may be able to deduce the amount of brine shrimp that can sustain a swarm of mysids. Also, we may be able to observe the behaviors of specific species of mysid shrimp.Why SURF?: I wanted to be able to expand my science horizon, and be able to get a feel for what professional research is like. I felt like the SURF program was a great opportunity for me. I am gaining valuable insight and experience this summer. It is giving me a feel of what the world of science is like outside of school. Also, it is giving me a feel of what macrobiology research is like as opposed to microbiology. The SURF program is an excellent opportunity for all undergraduate students.Memorable research moment: We were able to go into the field in the Gulf of Mexico in a number of sites including Mississippi and Florida. It was an awesome mix of working and being a tourist in a beautiful part of the country.!!

Rhode Island NSF EPSCoR SURF 2014!David Gleeson | University of Rhode Island!

Coral Restoration and Lionfish Study, British Virgin Islands!!Mentor: Dr. Graham Forrester!Major: Marine Biology!Career path: Conservation!Expected graduation: Spring 2016SURF project goal: Continuing previous research that has been conducted on coral reefs in the British Virgin Islands. We hope to gain a better understanding of how coral fragments respond to a specialized care environment, essentially a nursery. Ideally, these nurseries will be used to increase the speed at which damaged coral reefs can recover. While in the BVIs, I will be studying how lionfish behave around divers and looking into the effectiveness of lionfish removal programs. I expect to see a difference in lionfish behavior between areas frequented by divers and areas where lionfish are left alone.!Why SURF?: The opportunity to travel to the British Virgin Islands and conduct research on coral restoration. The chance to conduct my own study on lionfish was a bonus, as I’m interested in fisheries conservation.The experience has been very positive. Early on, most of the work has been to compile data that we will use during the lionfish study, but in a few weeks I will be SCUBA diving every day, studying the coral and the lionfish. The experience I’m getting out of the program is fantastic.!Memorable research moment: The first time we found lionfish along one of our research transects was incredible. Despite being bad for the reef, lionfish are incredibly beautiful underwater. My dive partner and I found a small cave under our transect line that held three fish inside, and we were able to observe their behavioral responses toward us.!

Rhode Island NSF EPSCoR SURF 2014!Sean Grace | Rhode Island College!

Heat shock proteins in Geukensia demissa as indicators of climate change andenvironmental stress on marine life in Narragansett Bay!!

Mentor: Dr. John Williams!Major: Biology!Career path: Evolutionary Biology!Expected graduation: Spring 2015SURF project goal: The organisms we are studying — the Atlantic Ribbed Mussel or Geukensia demissa — produce proteins when they are exposed to high temperatures to protect themselves. These are called heat shock proteins. This summer, we will be developing a procedure to measure the amount of heat shock proteins being expressed by mussels in the field. With these data, we plan to compare heat shock protein levels to temperatures in the bay to monitor climate change and its effect on the health of the local ecosystems. We expect heat shock protein levels to rise with temperature. Why SURF?: I wanted to participate in the SURF program to gain experience working in a laboratory on an ecology project. I am learning new techniques and participating in problem-solving discussions.Memorable research moment: My most memorable moment this summer so far is when we first started getting good results from our experiment. !

Rhode Island NSF EPSCoR SURF 2014!Elizabeth Groover | Roger Williams University!

Ocean acidification effects on fish larvae!!Mentor: Dr. Andrew Rhyne!Major: Biology, with a minor in Aquarium Science!Career path: Fish husbandry research!Expected graduation: Spring 2015SURF project goal: The main purpose of our research is to discover how ocean acidification (gradually decreasing pH) may be affecting otolith (inner ear bones) development in reef fish larvae and how this may affect their ability to orientate themselves in a 3D marine environment. It is expected that otolith morphology will be altered due to decreasing pH, which will negatively affect larval fish in their ability to orientate themselves and therefore survive to later life stages. Why SURF?: After working with fish larvae for research that I conducted while studying abroad in Bonaire last fall, I wanted to continue to gain experience, performing research focused on fish larvae. This SURF project was the perfect opportunity. I also thought this would be a great way to learn how to perform research alongside other scientists and improve upon my lab skills. I have been able to experience firsthand how a complex and sophisticated research project is conducted, as well as acquire many new lab techniques, such as extracting otoliths from larval fish using a polarizing microscope.Memorable research moment: The day that we were all able to successfully use the newly fixed polarizing microscope to find otoliths in the smallmouth grunt larvae. It was truly amazing to see the tiny ear bones glowing under the polarized light of the microscope for the first time.

Rhode Island NSF EPSCoR SURF 2014!Joseph Guerriero | Rhode Island College!

The Bermuda Seaweed Project!!Mentor: Dr. Chris Lane!Major: Biology!Career path: Teaching!Expected graduation: Spring 2015SURF project goal: We are using genetic markers to classify collected field samples. My research is specifically focused on working with red algae in the cryptonemia genus. Using specific genetic markers, we are not only classifying some samples that were previously collected, but we also are trying to get an idea of how the different species of cryptonemia have evolved over time and over different collection sites. We are expecting to at least get the genetic data by the end of the summer, and hopefully begin working on assembling evolutionary trees based off of the genetic data we will collect during the summer.Why SURF?: I participated in the SURF program last year, doing a more fieldwork intensive project looking at salt marsh grass and ribbed muscle relationships, and I really enjoyed it. This year I wanted to work on a project that was more focused on work in the lab and molecular techniques and the SURF program was the best option for getting critical research experience and get paid to do it. There have definitely been moments of frustration as I have been working with samples that have given some of the other researchers trouble in the past, however, I do find it incredibly satisfying when I finally get things to work right. I believe that I am getting a more profound understanding of what it is like to work in a lab day in/day out and to take the details of how and when to complete aspects of my project onto myself and get things done on my own terms. Memorable research moment: When I finally got my first set of good sequence data. After a few weeks of getting nothing useful, it was a great feeling to finally be able to jump into some of my data.!!

Rhode Island NSF EPSCoR SURF 2014!Jennifer Kane | Salve Regina University!

The effect of climate change on near shore invertebrate and fish populations!!Mentor: Dr. Jameson Chace!Major: Biology!Career path: Marine biologist!Expected graduation: Spring 2016SURF project goal: We are researching the effects climate change will have on the abundance and distribution of near shore invertebrate and fish populations around Newport Neck. We have lobster traps along Newport Neck that we check every three days and record the species, sex and size of what we find in the traps. We also conduct intertidal, subtidal and supratidal surveys to record what is available now and what will be available after sea level rise has made an impact on the RI coast. After we have analyzed the past five years of data, we will be able to predict the effect climate change will have on the near shore substrate and the habitat selection species will have to choose from.Why SURF?: I wanted to get involved with the marine research that was offered through the program and get more experience with fieldwork. My experience has been amazing. I am learning new field techniques everyday and I love getting a chance to interact with the species we are working with each day. Memorable research moment: Recently, we caught our first American Eel of the season in one of our traps. It was a very exciting and memorable experience.

Rhode Island NSF EPSCoR SURF 2014!Elizabeth Kawa | Providence College!

The feeding behavior of Opossum shrimp!!Mentor: Dr. Jeffrey Markert!Major: Biology!Career path: Medical field!Expected graduation: Spring 2017SURF project goal: We are attempting to observe the difference in feeding rates between male, female, and juvenile Opossum shrimp. In addition, we have performed many behavioral assays to explore the interactions and movement patterns between the various species of Opossum shrimp. The data received from the behavioral assays potentially could correlate to the amount the shrimp consume. I hope to have consistent results that will determine the largest consumer of food among the Opossum shrimp and to eventually relate that data to that which we receive in the behavioral assays.Why SURF?: It’s an excellent opportunity to explore my own interests in the field of biology and to discover how science operates in the real world. I thought that partaking in a research project would lead me to change the direction I take for a career after college. This program could open numerous doors for me when applying to medical school or future jobs. This project has allowed me to become more self-reliant, enhanced my critical thinking skills tremendously, and developed my leadership skills a great deal. It has taught me to look at data more effectively and with a more critical eye.Memorable research moment: Traveling to the Gulf Coast to collect various species of Opossum shrimp and sorting them in the Environmental Protection Agency located in Pensacola, FL.

Rhode Island NSF EPSCoR SURF 2014!Jacqueline Kratch | Bryant University!

Systematics of trace metal transport and bio-uptake in urban intertidal zones, Narragansett Bay!!Mentor: Dr. Julia Crowley-Parmentier!Major: Biology!Career path: Research!Expected graduation: Spring 2015SURF project goal: We are trying to determine the level and different heavy metal pollution in different beaches along the Providence River and how much local plant life takes up. Some of the expected outcomes of the project are that the beaches by the industrial sites will be more contaminated than the one that is closer to the start of the river. Looking at different samples, it also is predicted that the native plant life that lives close to the pollution would take up metals from the environment.Why SURF?: To gain the experience of working in the field and working on my own project. I am getting good experience out of the program from working in a lab and I’m learning more from the project being hands-on than classroom learning.!Memorable research moment: Sampling the different locations along the river and seeing what we would be studying was one of the most memorable experiences of the project.

Rhode Island NSF EPSCoR SURF 2014!David Lam | Roger Williams University!

Can we synthesize structurally diverse pyrazolines to better understand the requirements for inhibition of amebic growth and enzymatic activity?!

Mentor: Dr. Lauren Rossi!Major: Biology & Chemistry, with a minor in Math!Career path: Physician!Expected graduation: Spring 2016SURF project goal: I am trying to create a variety of different chemical compounds that hopefully have antiamoeba growth properties. Once these compounds are successfully made, they will be tested on amoebas to see which chemical compounds are more successful in stopping amoebas from growing. I intend to have my library of compounds that were synthesized be analyzed for their different chemical properties, and to have results on the varying inhibition abilities of each compound from the amoebas they were tested on. Why SURF?: I wanted to feel firsthand what it means to be a researcher in a lab instead of being a student in a lab. I’ve never had any sort of research experience thus far in my undergraduate experience. So, this was a springboard to launch my research and scientific career, and to gain valuable experience in this particular aspect of scientific academia. This summer has given me insight into what research is like and the things a researcher needs to go through in order to get results. I love being able to work with my mentor and learning little tips and tricks that tweak my own technique in lab to make me more proficient. !Memorable research moment: At the beginning, I was just awed with all the new equipment that would be available for my use in order to work up the different reactions that I would be doing — especially the Rotovap. When I first started using the Rotovap, thinking how cool it was, I expressed my thoughts on it. One of my labmates overheard and told me, “It stays cool for about the first day, just wait.” After using the Rotovap more times than I can count now, though, he’s right. Still, learning how to use the lab equipment definitely was a highlight, along with finally getting through an experiment and making my desired product.

Rhode Island NSF EPSCoR SURF 2014!Stephaine Liptak | Salve Regina University!

How does the gene expression change in Ulva blooms?!!Mentor: Dr. JD Swanson!Major: Biology!Career path: Possibly in the genetics field!Expected graduation: Spring 2016SURF project goal: We are investigating the gene expression involved in algal blooms from samples collected at the beginning, middle, and end of summer. Looking through the transcriptome analysis of each sample will allow us to compare the gene expression across two species of Ulva and see which genes are turned on the most and at what point in the summer they are turned on. Based on this data we will be able to tell which genes are involved specifically in the blooming month of July. We expect to find specific genes which are correspondent to the Ulva blooming season. Then we can look into the pathways that are effected by the up-regulation of these specific genes and based on this we can develop a way to regulate this gene and control the blooming of Ulva species around the world.Why SURF?: So I could have the opportunity to get hands-on experience with research that allows me to learn and investigate a problem. To find the solution to the problem we are approaching, I am learning techniques and skills that I can bring to other research projects. This summer has been awesome because this project has given me the opportunity to learn a ton of scientific techniques like DNA/RNA extractions, PCR, qPCR and data analyzing and more. I also have had the opportunity to work as a team with my colleagues to brainstorm new ideas and troubleshoot any problem we run into. It has been rewarding to apply the science that I know to something bigger than a test.!Memorable research moment: Collecting Ulva samples and freezing them in liquid nitrogen in order to extract their DNA or RNA!

Rhode Island NSF EPSCoR SURF 2014!Jillon McGreal | University of Rhode Island!

Sea Star Wasting Disease!Mentor: Dr. Marta Gomez-Chiarri!Major: Marine Biology!Career path: Research scientist, professor!Expected graduation: Spring 2015SURF project goal: Sea stars on the west and east coasts are dying due to what is referred to as “sea star wasting syndrome.” Sea stars infected with this will become less turgid, get white mucus on the surface of their skin, form lesions, and sometimes their body tissue will ulcerate; it almost looks as though they are melting away. The problem is, no one knows what is causing this! The overall goal is to find the culprit, but there is so little known about the sea star wasting syndrome that any observations or new information is valuable. My project is testing whether the pathogen, or the responsible agent, is a virus or bacterial in nature. (I plan to continue studying the sea star wasting syndrome with URI graduate student, Caitlin DelSesto, after the end of my SURF internship.)!Why SURF?: It’s a great way to gain research experience and create professional relationships. There are field trips, workshops, and seminars throughout the program, which makes for a more well-rounded experience. I got to perform my own study that was only a month long in Bermuda last fall, when I was involved in a marine science intensive study abroad program. The SURF program was the perfect opportunity to take that next step toward becoming an experienced scientist. I feel more comfortable and confident in a lab setting. I am more confident in myself as a scientist; I am feeling less like a student and more like a coworker/peer and I am less afraid to speak up if I have an idea. Being involved in this program has made it clear that I am definitely pursuing the right career path. I love research!!Memorable research moment: One of my favorite things about my research is that the more I learn, the more I realize we don’t know; I get so excited thinking about possible future projects!!

Rhode Island NSF EPSCoR SURF 2014!Alexandra Moen | University of Rhode Island!

Mechanisms behind the production of nitrous oxide from marine invertebrates!!Mentors: Dr. Serena Moseman-Valtierra, Melanie Garate!Major: Marine Biology!Career path: Marine research/ diving safety officer!Expected graduation: December 2014SURF project goal: I am trying to further my experience in the field of marine research and gain a better understanding for life as a graduate student. I expect to have a better understanding about the field of marine research and to have an idea of whether or not graduate school is a possibility in my future.Why SURF?: I wanted to work under a mentor who has been successful in my field at a reputable institution studying something I am interested in. My SURF experience has been full of knowledgeable and stimulating experiences ranging from fieldwork to techniques in the lab. I am gaining a better understanding and appreciation for graduate students and their work as well as the overall field of marine research. Memorable research moment: Finding large mantis shrimp in Mt. Hope Bay and attempting to catch them. !!

Rhode Island NSF EPSCoR SURF 2014!Heather Nicholson | Salve Regina University!

How will sea level rise affect abundance and distribution of near shore invertebrate and fish populations?!!Mentor: Dr. Jameson Chace!Major: Biology, with minors in Environmental Studies & Chemistry!Career path: Conservation, marine biology or environmental science!Expected graduation: Spring 2015SURF project goal: Determine the abundance and distribution of various marine benthic macro invertebrates and small fish and their habitat associations with different types of substrate along Newport Neck. We do this by setting lobster traps, taking intertidal quadrat surveys of species at low tide, and surveying the percent coverage of different substrates at multiple supratidal and subtidal sites along Newport’s Cliff Walk and Ocean Drive. As sea level rises due to the effects of climate change, we hope these data will allow us to predict how these marine organisms will be distributed when the current supratidal zone becomes the new intertidal zone. I expect we will find certain species to be more prevalent in some areas than others, and that sea level rise will have a significant impact on the distribution of intertidal marine organisms, because much of the supertidal substrate that is expected to be underwater in the future manmade and may not be fitting for these species. Why SURF?: It is an amazing opportunity to work in a field I am interested in, make numerous networking connections with other scientists and professors, and live in Newport for the summer while undertaking true field work concerning climate change. It is a rare opportunity to gain experience in a field that I would love to work in someday. I am realizing that research does not necessarily mean working day-to-day inside a lab — it also can be a much more hands-on career. !Memorable research moment: Seeing the way our team reacts to pulling up a lobster trap that has caught one or more eels, which are typically the length and width of a human arm. This usually involves some screaming, falling, and, thankfully, at least one person who can reach inside the trap to remove any crabs or lobsters while the eel is still slithering around inside, or even worse, trying to escape.!

Rhode Island NSF EPSCoR SURF 2014!Katie Nickles | University of Rhode Island!

Understanding the evolution of parasitism in red algae!Mentor: Dr. Chris Lane!Major: Marine Biology!Career path: Research!Expected graduation: Spring 2017SURF project goal: The first part of my SURF project is to identify all the genes on a red algal chloroplast plastid. This species of red alga has a very closely related parasitic red alga that targets its cells. The parasitic alga uses the host’s chloroplasts and has none of its own. So our purpose is to determine which genes the parasite possess to be able to still use the chloroplasts of the host. Once we know what genes it uses, we would be able to target those genes to prevent the parasite from spreading. I expect to see that the genes that were transferred from the plastid to the host red alga will also have been transferred to the parasite red alga so they both have the ability to control and run the chloroplast. Why SURF?: It’s a great opportunity to get some real lab/field experience. The SURF program also has the poster presentation at the end of the summer and I have never presented a poster, but it is something I would like to do. The program also provides the opportunity to be a part of a larger research community. It allows us to see what other people are working on, and exposes us to more research in interesting fields. I have spent a lot of time in the lab learning about algae, PCR, DNA, and parasites. The lab processes we are using, PCR and gel electrophoresis, are very valuable for any future lab work. Also, working in this lab has made me realize that looking at things at the DNA level is very effective and can be very helpful for other types of research. Memorable research moment: When I asked why. Once the entire picture of the research was explained, the process made so much more sense. The purpose of identifying genes on the red algal plastid is to allow us to determine what genes are necessary for both the host and parasitic red algae to exist. Once we understand the parasitic red algae, we can transfer that knowledge to help with parasitic-based diseases like Malaria.

Rhode Island NSF EPSCoR SURF 2014!Christine Ortiz | Salve Regina University!

Differentiation of pathogenic Leishmania species through amplified fragment length polymorphism!!Mentor: Dr. Alison Shakarian!Major: Biology!Career path: Physician!Expected graduation: Spring 2015SURF project goal: We look at the genetic differences in a series of species from the same genus of infectious parasites known as Leishmania to determine what genes are associated with the pathogenicity of certain species. We expect to see genes expressed in the pathogenic species that are not present in to non-pathogenic species of Leishmania. Why SURF?: The SURF program has been a great way to get more experience in the research field at my university. This summer, I am much more skilled than I was my first summer doing the SURF program. Techniques that used to be difficult for me are now a lot easier to do because of the amount of practice I get with the techniques. I am also learning about other research projects and building some knowledge on those as well as my own.Memorable research moment: Getting the protocol down for our gel electrophoresis analysis of the Leishmania genes has been the most memorable moment for me in research. It feels great when protocols are optimized for the best result yield! !

Rhode Island NSF EPSCoR SURF 2014!Christina Perry | Providence College!

What are the mechanical properties of the zebra finch shoulder girdle? !!Mentor: Dr. David Baier!Major: Biology & Psychology, with a minor in Neuroscience!Career path: Pediatric neurologist!Expected graduation: Spring 2016SURF project goal: Prior studies (Jenkins et al, 1988) have suggested that the furcula, or wishbone, in small and medium birds may function as a spring during flight, spreading during downstroke and recoiling during upstroke. Using biplanar X-ray videography of zebra finches in flight and CT scans of the finches, we will be able to generate three-dimensional computerized models to examine movement of the shoulder girdle during flight and determine when during flight the furcula is spreading. The exciting thing about this research is the variety. While Jenkins et al found that the furcula of starlings spreads during downstroke, other research in pigeons and chukar partridges has suggested the opposite – that it recoils during downstroke. Who knows what we’ll find!Why SURF?: It’s very rewarding to know that the project I am working on is part of a bigger entity. Even though I spend my day-to-day work primarily in a computer lab with one other lab mate and my PI, I know there are thirty-nine other students working on projects in EPSCoR’s SURF program. Being able, as an undergraduate, to say that I am, and we are, adding to the body of scientific knowledge while working in a research lab (and not just cleaning beakers) is incredible.Memorable research moment: After spending days cutting bones out of the CT scan images in a very finicky program, digitally cleaning up the models in a different program, and doing calculations of each bone’s inertia in a third program, I was finally able to import a three-dimensional model into our animation software. Seeing a bird’s skeleton materialize in three-dimensional space – vertebrae, wings, sternum – after so much time put in to get there was wonderful.!

Rhode Island NSF EPSCoR SURF 2014!Dillon Post | Community College of Rhode Island!

Ocean acidification effects on fish larvae!Mentor: Dr. Andrew Rhyne!Major: Biology!Career path: Undecided!Expected graduation: Spring 2017SURF project goal: This project aims to further the understanding of how ocean acidification (decreasing pH) affects the otoliths (ear bones) of larval Clarkii Clownfish. This experiment adds a variable of diet to see if the food source effects fish development in relation to the lowered pH. !Why SURF?: I wanted to take my interest in Biology out of the classroom and find what “real world” research was like, and if it was a path for me to follow. The program has given me a great understanding of how research is conducted and how many institutions are often involved in one project. I work alongside fellow undergraduate students, graduate students, professors, lab managers, and marine biologists, and use technical equipment not many undergraduate students are allowed access to. Being immersed in this project has given me the opportunity to perform work that affects our understanding of how human interaction is changing the ecosystems of the Earth, and the wildlife. Working alongside such experienced and devoted individuals, and being allowed to carry out important responsibilities, has given me a sense of honor in being a SURF student.Memorable research moment: After performing a number of micro-dissections under a microscope, I had not yet been able to locate the smallest of the three sets of otoliths, the Asteriscii. It was already explained to me how difficult they were to find and remove. I approached the practice specimens with confidence, and expected to pull out the Asteriscii. After searching through many specimens, my hope began to dwindle and I was almost certain I would never be able to find one of the Asteriscii, which are only visible on the highest magnification of the polarizing microscope. I was thrilled to finally find one of these microscopic otoliths in one of the final specimens of the day. After many more hours of practice I was able to pull them out with no problem, and enjoyed every minute of the dissection process.!

Rhode Island NSF EPSCoR SURF 2014!Ryan Quinn | University of Rhode Island!

Greenhouse gas fluxes from biological nutrient removal at Field’s Point Wastewater Treatment Plant!!Mentors: Dr. Serena Moseman-Valtierra, Elizabeth Brannon!Major: Biological Sciences!Career path: Scientist and/or teacher!Expected graduation: Spring 2015SURF project goal: We want to better understand the role photosynthetic organisms have on greenhouse gas fluxes from biological nutrient removal at Field’s Point Wastewater Treatment Plant in Providence, RI. I hope to learn how to use lab equipment like how to use a gas chromatograph and spectrophotometer. I also want to learn how to use field equipment like a redox probe, and the Picarro, a new machine that measures N20, CO2, and CH4 fluxes in real time. Why SURF?: I love research and always wondering about the world around me. I want to become a more competent researcher by working for and alongside graduate students. I want to gain as much experience in the lab and field as possible. It’s been a great experience. I am learning new, interesting things every day. I understand the science behind the Picarro, know how to use a gas chromatograph, and just finished carrying out a 24 hour incubation to determine the effects of algae on greenhouse gas fluxes. Memorable research moment: Splashing wastewater onto my jeans at the beginning of a long field day at the wastewater treatment plant. !

Rhode Island NSF EPSCoR SURF 2014!Oscar Robert | Rhode Island School of Design!

How can public understanding of marine ecosystems be enhanced throughnovel representations of environmental monitoring data?!!

Mentors: Neal Overstrom, Dr. Susanne Menden-Deuer, Dr. Sunshine Menezes!Major: Graphic design!Career path: Pixar, Facebook, or any startup design company!Expected graduation: Spring 2016SURF project goal: We are trying to convey the detrimental effects that algal blooms cause to our ecosystems using a narrative device to stimulate engagement and learning. I expect that we will be able to create a web experience that simplifies the message to the core of what the public is able to understand.Why SURF?: I was encouraged to apply to the fellowship program due to my experience in data visualization.!Memorable research moment: It’s hard to say what my most memorable experience has been. As an artist, it took me a while to adjust to the research mindset. I think what I have gotten out of this experience is the ability to talk to scientists at a very fundamental level to understand some of the frustrations that both disciplines have with one another and to work them out until we are able to find the answers to our problems.

Rhode Island NSF EPSCoR SURF 2014!Noah Schlottman | Brown University!

Can novel representations of living marine plankton foster public in and understanding of marine ecosystems?!!Mentors: Neal Overstrom, Jennifer Bissonnette!Major: Environmental Science & Music!Career path: Research and/or conservation work!Expected graduation: 2016SURF project goal: To represent living marine plankton in a way that incites curiosity and compels people to engage with marine ecosystems. We are mainly focusing on different forms of narrative and how charisma, influenced by form and context, may help us and other science communicators craft more influential science (specifically plankton) stories. I expect that we will have developed elements of compelling narratives for organisms and topics not typically charismatic or tangible, like marine plankton.Why SURF?: I’ve grown up with a deep love of the ocean, which has guided my interests, hobbies, and my extracurricular experiences and educational career. My fascination and knowledge of the sea has been accompanied by a desire to communicate what I know and love about the ocean to others in a way that allows others to share in my wonder. Science communication has similar goals, as it is the hope that wonder and fascination can foster stewardship, investment, and care for the natural world. I was drawn to the SURF program because of its intensive focus on conducting relevant marine science research and in addition searching for effective and novel ways to communicate that research to a wider audience.Memorable research moment: Finding myself always making sound effects whenever I look at plankton under the microscope, which has inspired ideas for novel representations as well as allowed me to connect with a certain childish fascination that plankton can evoke!!

Rhode Island NSF EPSCoR SURF 2014!Abigail Scro | Roger Williams University!

What are the implications of climate change on the population dynamics and biotic interactions among winter flounder, summer flounder, and blue crabs?!!

Mentor: Dr. David Taylor!Major: Marine Biology, & Math!Career path: Undecided, possibly graduate school!Expected graduation: Spring 2015 SURF project goal: I have been working toward setting up a protocol to look into blue crab predation on winter flounder in the Narragansett Bay. We are looking to detect winter flounder in the stomachs of blue crabs through genetic analysis. We expect to determine the sensitivity and specificity of our winter flounder specific primer pair used in the pcr (polymerase chain reaction) protocol. Sensitivity will be analyzed through blue crab feeding trials, where the crabs will be sacrificed at different time intervals after feeding on winter flounder. We also have and will continue to test the primer pair against a range of possible other blue crab prey, including both vertebrates and invertebrates. Why SURF?: I was interested in the different opportunities the SURF program provided, including being able to present my research at the end of the summer. I have gained a better understanding of the scientific research process and I’m learning various lab techniques that I hope will carry on into a future career. On top of the lab work, I have done a great deal of field work, which I have come to love.!Memorable research moment: Fishing in the Narragansett Bay.

Rhode Island NSF EPSCoR SURF 2014!Mahrukh Shaikh | University of Rhode Island!

How do microbe-mineral interactinos in Earth’s oceans respond to climate change?!!Mentor: Dr. Dawn Cardace!Major: Geosciences!Career path: Geologist!Expected graduation: Spring 2016 SURF project goal: Microbes are the single cell organisms that are present just about everywhere including oceans. Using the deep-sea bedrock called serpentinite, I am researching how these microbes will adjust/adapt to the ocean acidification in different regions of the ocean. This will be accomplished by immersing very thin serpentinite mineral films (wafers) in the seawater samples. These prepared samples will be studied under conditions of light, dark, ambient carbon dioxide, increased carbon dioxide, & anaerobic conditions to mimic different regions of the oceans and studied to see how the microbes present in the sample will behave under these changed conditions. I expect this experiment to provide us with some insight into how the microbial communities will respond to the changing oceanic conditions. This can help us to predict the microbial trend in both the benthic and pelagic microbial communities that make up the bottom of our food chains. Why SURF?: It is a great opportunity for me to work as a researcher under the mentorship of my professor and broaden my understanding of the research processes. I also wanted to get a feel of the graduate school and SURF provided me with an excellent opportunity. Memorable research moment: I began this experiment with the impression that it was a simple ocean acidification experiment, but after hours and hours of journal readings, I realized that what I was researching was something extremely vast and tremendously significant. This seemingly simple experiment will provide us a window of how our entire food chains will be affected in the future due to ocean acidification. That for me was the wow moment! !

Rhode Island NSF EPSCoR SURF 2014!Beatrice Steinert | Brown University!

Can novel representations of living marine plankton foster public in and understanding of marine ecosystems?!!!Mentors: Neal Overstrom, Jennifer Bissonnette! Major: Biology & Science and SocietyCareer path: UndecidedExpected graduation: Spring 2016

SURF project goal: To understand how to present information about marine plankton in a way that will trigger an emotional reaction in people. More specifically, we are focusing on how to craft engaging narratives and determining how charisma or plankton “personalities” might play a role in how people engage or empathize with these organisms. We are still in the process of determining the final form our project will take. Right now, we are focusing on collecting observations and exploring ways of constructing narratives. Our hope, however, is that we can get people to see these important organisms in a new way. Why SURF?: I wanted to participate in this unique project at the RISD Nature Lab, which allows for exploration of communication as it applies to both art and science and the intersection of the two. In addition to admiring the elegance of biological systems and structures, I have always been attracted to the imagery they have the potential to produce because it can be at the same time incredibly beautiful, communicative, and educational. Visual information and communication is such an important part of our everyday lives, but is not often directly talked about outside the realm of art and design, especially in science classes. Since our interactions with the world around us are primarily visual, one of the most effective methods of communicating science and scientific research, which is in pursuit of a deeper understanding of the world, is to use the same language with which we primarily experience it.Memorable research moment: Teaching myself to identify different types of zooplankton with just a strong light and my eyes has been particularly memorable. By concentrating and paying very close attention to subtle differences in movement, I can pick out certain zooplankton or recognize ones I have never seen before.

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Rhode Island NSF EPSCoR SURF 2014!Jake Swanson | Providence College!

Differential effects of temperature change on southern New England intertidal consumers!!Mentor: Dr. Patrick Ewanchuk!Major: Biology!Career path: Pursue Ph.D. and work in academia or industry!Expected graduation: Spring 2015 SURF project goal: The effect of the predicted rise in ocean temperature caused by climate change on many marine organisms is unknown. My project is looking at the effect of temperature on two different species of snails that can be found here in Rhode Island. Specifically, I am interested in whether a rise in temperature will cause one species to out-compete the other for resources that are essential for survival. When the temperature rises, I expect the more southern of the two species to out-compete the northern species. Why SURF?: I felt SURF would provide me with a unique opportunity to gain experience both in the field and in the lab while being able to work closely with distinguished faculty members and other students. My experience has been nothing but positive. I feel like I am gaining a lot valuable research experience that will be beneficial to me when I start my life after college; whether I continue my education or enter the workforce to do my own research. Memorable research moment: The first day I got to go into the field. I traveled with my mentor to Narragansett Bay to collect snails and mussels for my project. While it wasn’t the most beautiful day, I enjoyed being able to get out of the lab and work out in nature.

Rhode Island NSF EPSCoR SURF 2014!Megan Sylvia | Salve Regina University!How does gene expression change in Ulva bloom?!!

Mentor: Dr. JD Swanson!Major: Biology!Career path: Undecided!Expected graduation: Spring 2016 SURF project goal: Gain a greater understanding of the Ulva blooms that are occurring in Narragansett Bay. Ulva is a type of green algae found natively in this region. We are specifically examining the two species Ulva compressa and Ulva rigida. During the summer, large Ulva blooms occur, damaging the Bay ecosystem by creating large dead zones. We expect that the genes responsible for the large Ulva blooms found in the summer months are up-regulated by higher temperatures and longer photoperiods and down-regulated by lower temperatures and shorter photoperiods. Why SURF?: I wanted the opportunity to work in a lab full time instead of trying to fit in research between classes, and to learn new and valuable research techniques that will help me find a career in the science field. I’m getting practical research experience, performing experiments where the outcome is unknown, instead of having designed experiments, like in school, with pre-determined outcomes. I am actually trying to figure out something new about an organism. SURF has given me valuable experience, not only with scientific research methods but also with understanding how to troubleshoot my experiments and determine the next step on my own. Memorable research moment: Collecting algae samples with fellow student researchers from our three collection sites surrounding Narragansett Bay. It was a fun day, and interesting to see where the Ulva I have been researching comes from. !

Rhode Island NSF EPSCoR SURF 2014!Leigha Tacey | Bryant University!

Wood pellet combustion contamination!!Mentor: Dr. Dan McNally!Major: Biology!Career path: Occupational therapy or research!Expected graduation: Spring 2015 SURF project goal: Find out what trace metals and organics (PAHs), which are all contaminates, can be found within the wood pellets and with the combustion of them. These are harmful to vegetation growth and some are carcinogenic and can be harmful if handled improperly. There is not much research on this topic, which is exciting. I expect to find that the combustion of the wood pellets, the ash and tar will have a lot of PAHs, which are carcinogenic contaminates, and that there will be trace metals in all the samples because of the vegetation uptake of the trees used to make the wood pellets. Why SURF?: I thought it would be a good experience to learn lab practices and to discover whether I enjoy working in a lab setting. I am discovering a lot about interesting topics and the use of lab equipment. The experience is helping me learn more about myself and my interests in the environmental aspects of science, and it is pushing me toward my research career goals. Memorable research moment: When we got the GC-MS to start working so I could get data for my wood pellet project. I also enjoyed field sampling on the beaches in Providence with SURF student Jackie Kratch and her mentor for her project.

Rhode Island NSF EPSCoR SURF 2014!James Tempest | Community College of Rhode Island!

How is protein expression of marine invertebrates affected by projected ocean temperature and pH changes?!!Mentor: Dr. Steven Irvine!Major: Biology/Chemistry!Career path: Study biology and chemistry; find a fun and challenging career that allows travel and spending time on the ocean Expected graduation: 2016 SURF project goal: We study a common sea squirt Ciona Intestinalis by collecting them from a local marina and performing protein and RNA extractions. These animals have already had their entire genome sequenced and they have interesting properties for research. They are seemingly simple, but are actually quite fascinating. I will never look at them the same way again. As a fisherman, I tend to look for vertebrate fish and did not even know what a Ciona was until this summer. We are looking at the RNA and proteins, and how the protein expression of these animals changes under various conditions. Why SURF?: I earned my GED in 2010 and thanks to my hard work and the wonderful professors at CCRI, I discovered my true calling was science. I completed CCRI’s Biotechnology program and applied to the SURF program. SURF is a great opportunity to utilize my recently learned biotechnology and chemistry skills, and network with other passionate scientists. It seems like a dream to arrive at beautiful campuses and do real research with state-of-the-art equipment after having worked long factory hours and studying nights and weekends. !Memorable research moment: Each day I walk through URI’s greenhouse and garden area to get to my lab might be the most memorable. But, one experience in particular stands out — I was working at URI’s Graduate School of Oceanography and helped unload sharks. These dogfish sharks are a common catch for any Rhode Island angler and I have caught them before, but it was especially fun to carry them into the aquarium and learn more about them. !!

Rhode Island NSF EPSCoR SURF 2014!Megan Warburton | Rhode Island College!

How is protein expression of marine invertebrates affected by projected ocean temperature and pH changes?!!Mentors: Dr. Steven Irvine, Dr. Thomas Meedel!Major: Biology!Career path: Working with marine mammals!Expected graduation: Spring 2015 SURF project goal: Ocean temperatures are projected to be increasing in the next few years. Our project looks to determine how this temperature increase will affect the protein make up of the sea squirt, Ciona intestinalis. We also are looking at the reproductive success of animals in higher temperatures versus control animals. We expect that various proteins such as heat shock proteins will increase in the higher temperatures. We also expect that animals raised in higher temperatures will have lower reproductive success than those raised at standard temperatures. Why SURF?: It’s an amazing experience that will prepare me for a future in the field of Biology. I think that the best way to learn Biology is through hands-on experience, and I have learned so much working with Dr. Meedel and Dr. Irvine. I have met wonderful people and been exposed to all sorts of new experiments and equipment. And, I have expanded my knowledge of experimental processes, which will be extremely beneficial when I begin a career. Memorable research moment: Finishing our first PCR. Throughout all of my biology courses I’ve learned all about PCR and it was amazing to actually finally complete one. I am extremely honored to be included in this project. I can’t wait to present my project at the SURF conference at the end of the summer.

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