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    Summative Grade 11 AP Biology

    Prokaryotes

    Prokaryotes are extremely common

    Can be found almost everywhere (bacteria)o Soil

    o Air

    o On organisms/in them

    o Every possible place that has favourable living conditions

    Archaea can also be found in extreme conditions

    Their collective mass is at least 10 times that of all eukaryotes

    Bacterial Cell Wall

    Formed by peptidoglycan

    Stain either Gram-positive or Gram-negative (see classifying bacteria)

    Peptidoglycan encloses the entire bacterium and anchors other molecules that

    extend from its surface.

    Difference from plant cell wall: plant cell walls are formed mainly by cellulose

    Classifying Bacteria

    Gram stain, developed in the 19th century

    Gram-positive bacteria have similar walls with a relatively large amount of

    peptidoglycan (thick protein layer on their cell wall and stains purple)

    Gram-negative bacteria have less peptidoglycan and are structurally more complex,

    with an outer membrane that contains lippolysaccharides. (carbohydrates bonded to

    lipids.) (thin protein layer on their cell walls and stain pink) Classified by shape, structure of their cell walls, and their source of food and

    energy

    Bacterial phylogenies have been constructed based on analysis of RNA sequences

    Genetic change by mutation occurs rapidly in bacteria

    Genetic differences can arise in bacteria populations within a few generations

    Bacterial Locomotion

    Flagella- may be scattered over the entire cell surface or concentrated at one or bothends of the cell

    Taxis- movement toward or away from a stimulus, chemotaxis- respond tochemicals by changing their movement patter

    Ex: movement toward nutrients/oxygen (positive chemotaxis), away from toxic

    substances (negative chemotaxis)

    Bacterial Reproduction (asexual)

    Binary Fission

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    Bacteria makes copy of its single chromosome, and when cell reaches a certain

    size, it elongates and separates the two chromosomes (chromosomes attached tocell wall by cytoskeleton). The cell then builds parition between them and the

    septum forms

    Septum is completed and distinct cell walls form

    Genetic Modifications of Bacteria

    Conjugation

    Two bacteria cells are linked to each other through pilus (bridging structures)

    One bacterium transfers all or part of its chromosome to another across the pilus

    Receiving cell undergoes binary fission to produce more cells with the new gene

    contentTransformation

    The modification of genotype of a cell by the introduction of DNA from another

    source

    Ex: plasmid (small loops of DNA separate from the main chromosome), contains

    one to few genes, these genes are different from those found in the chromosomes,can split from the bacterial chromosome and rejoin it, important in producinggenetic recombination in bacteria

    Transduction

    Transfer of genetic material by phages (viruses) as they infect one cell to another

    Nutritional Categories of Bacteria

    Photoautotrophs

    Use light as their source of the needed energy and CO2 to form organic compoundsPhotoheterotrophs

    An organism that depends on light for most of its energy and principally on organic

    compounds for its carbonChemoautotrophs

    Use CO2 and oxidize inorganic substances to obtain energy

    Chemoheterotrophs

    Oxidation/consummation of organic molecules to generate energy and to obtain

    carbon

    Classification of Prokaryotes into two DomainsCarl Richard Woese defined Archaea in 1977 by phylogenic taxonomy of 16S ribosomal

    RNA, a technique pioneered by Woese. Molecular systematics is the use of the structure of

    molecules to gain information on an organisms evolutionary relationships. The study ofrRNA molecule led him to conclude that Archaeans are more closely related to Eukaryotes

    than bacteria, thus forming their own group.

    Ecological Significance of Prokaryotes

    Bacteria: decompose detritus and turn organic carbon into inorganic carbon

    Nitrogen fixing bacteria (nutrients for plants) and denitrifying bacteria

    Symbiotic relationships with eukaryotes

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    Key characteristics of the three domains

    Domain Characteristics

    Bacteria Cocci, bacilli, and spirals

    Most unicellular, some species form colonies

    Membrane-bound organelles absent Peptidolycan in cell walls

    Circular chromosome

    Nucleoid

    RNA polymerase: 1

    Rare introns

    Antibiotics inhibit growth (streptomycin and chloramphenicol

    Histones associated with DNA absent

    Initiator amino acid for protein synthesis: Formyl-methionine

    Membrane lipids- unbranched hydrocarbons

    Archaea

    Membrane-bound organelles absent Nuclear envelope absent

    Peptidoglycan in cell wall absent

    Membrane lipids : some branched hydrocarbons

    Several kinds of RNA polymerase

    Introns present in some genes

    Antibiotics dont inhibit growth (streptomycin and

    chloramphenicol

    Histones associated with DNA present

    Circular chromosomes

    Initiator amino acid for protein synthesis: Methionine

    Eukarya Nuclear envelope present

    Membrane-bound organelles

    Peptidoglycan in cell wall absent

    Membrane lipids: unbranched hydrocarbons

    RNA polymerase: several kinds

    Initiator amino acid for protein synthesis: Methionine

    Introns present

    Response to the antibiotics streptomycin and chloramphenicol:

    Growth not inhibited

    Histones associated with DNA present

    Eukaryotes

    Protists Diversity

    Most protests are unicellular, although there are some colonial and multicellular species.

    At the cellular level, many protists are the most nutritionally diverse of all eukaryotes.

    There are autotrophs, heterotrohps, and mixotrophs. There habitats are also very diverse.

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    Most protists are aquatic, and are found almost anywhere with water. They are important

    constituents of planktons. Reproduction and life cycles are highly varied. All three basic

    types of sexual life cycles are represented among protists, along with some variations thatdo not quite fit any of types. Asexual reproduction. Too many structural and functional

    differences among them.

    Genesis of eukaryotes from prokaryotes

    Endosymbiosis- origins of mitochondria and plastids(chloroplasts)

    - according to this theory, these organelles originated as separateprokaryotic organisms that were taken inside the cell as endosymbionts.

    Kindom Characteristics

    Protista Heterotrophs and autotrophs

    Most are single celled

    Eukaryotes

    Do not fit in other kingdoms

    Less diverse than bacteria, more diverse than other eukaryotickingdoms

    Fungi Heterotrophs- feed by releasing digesting enzymes into theirsurroundings, absorb the digested nutrients into their cells

    Few are unicellular

    Boundary of a fungal cell is a cell wall made of chitin insteadof cellulose

    Hyphae+septa

    Sexual+asexual reproduction

    Plants Vascular + non-vascular

    Autotrophs Roots, stems, leaves

    Alternations of generations

    Cellulose cell walls

    Have chlorophylls a and b

    Photosynthesis

    Aquatic or terrestrial

    Gymnosperms or angiosperms

    Multicellular

    Eukaryotes

    Mostly sexual reproducation

    Animalia Multicellular

    Eukaryotes

    Reproduce sexually

    Ingest food

    Aerobic

    Heterotrohps

    Herbivore, omnivore, carnivore

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    Kingdom Fungi

    Acquiring Nutrients

    Hyphae grows across a source of food (dead matter)

    Release digestive enzymes

    Enzymes break down large organic molecules in the substrate into smallermolecules

    Smaller molecules diffuse into the fungus for growth and repair (extracellulardigestion)

    More extensive the mycelium, greater surface area available for absorbing nutrients

    Some are parasites of plant and animals that specialized to feed on living cells, their

    hyphae is called haustoria that penetrate host cellsHyphae is a network of fine filaments. Mycelium is a loose, branching network of hyphae

    (Hyphae strung together form a network called mycelium)

    Fungal Cell Walls

    Chitin, a protein that makes up the cell wall of fungus cells

    Contributions to an EcosystemDecomposers: break down organic waste and release nutrients into the soil

    Performing essential recycling of elements between the living and nonliving

    worldThey form symbiotic relationships with plants, algae, and animals.

    We eat fungi.

    Use others to make cheeses, alcoholic beverages and bread

    Antibiotics produced by fungi treat bacterial infections

    Fungi is leading to applications in biotechnology

    Phyla of Fungi

    Phylum Characteristics

    Zygomycetes Saprotrophs, few parasites of protests and small invertebrate

    animals, mostly terrestrial, ex: Rhizopus stolonifer (black breadmold), Pilobolus (grows on animal dung)

    Basidiomycota Have short lived reproductive structure ex: mushrooms, rusts,smuts, shelf fungi/bracket fungi

    Ascomycota Largest group of fungi, have asci, mostly saprotrophs, parasites

    of plants, sexual reproduction ex: truffle, Aspergillus nidulans

    Chytridiomycota Water mould -Allomyces sap, Batrachochytrium dendrobatiolisBlastocladiomycota

    Neocallimastigomycota

    Glomeromycota

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    Kingdom Animalia

    Hypothesis for the origins of Animals

    Most systematics now agree that all animals lineages are monophyletic; that is, we can

    trace all lineages back to a single common ancestor. The ancestor was probably a

    flagellated protist; which was probably related to choanoflagellates.

    Two forms of symmetry of the Eumetazoa (clade comprising all majoranimal groups

    except sponges, placozoa and several other little known animals)Bilateral : has a left and a right side, one line of division that allow the body to be divided

    into equal but opposite parts

    Radial: parts of animal radiate from the centre. Any imaginary slice through the centralaxis divides the animal into mirror images

    Cephalization as an Evolutionary TrendThe nervous tissue, over many generations, becomes concentrated toward one end of an

    organism. This process eventually produces a head region with sensory organs. The endof a traveling animal that is usually first to encounter food, danger, and other stimuli.

    development of central nervous system and adaptation for movement on land.

    Germ Layers of Radiata and other Eumetazoa

    Radiata contain two germ layers including the ectoderm and endoderm, the otherEumetazoa differ in that they have an additional layer called the mesoderm.

    Classifying Animals

    Term Significance

    Acoelomates Animals with no blood vascular system and lacking a cavity

    between the gut and outer body wall ex: flatworm-major split in phylogenic tree for animal development involved the

    split of bilateral organisms into two further branches

    Pseudocoelomates Animals with fluid-filled body cavity that is not enclosed by

    mesoderm (body cavity formed from the blastocoel) ex:roundworms

    Coelomates A coelom is a fluid-filled body cavity found between the body walland gut that has a lining. It comes from the mesoderm.

    Protostomes Branches from Coelomates, a bilateral animal whose first

    embryonic indentation eventually developes into a mouth (mouthdevelops from blastospore) ex: annelids, arthropoda, mollusks

    Deuterostomes A branch from Coelomates that includes chordates andechinoderms. The first indentation of their embryos eventually

    develops into the anus (anus develops from blastospore)

    Blastospore: the indentation that during gastrulation leads to the formation of the

    archenteron

    http://en.wikipedia.org/wiki/Cladehttp://en.wikipedia.org/wiki/Animalhttp://en.wikipedia.org/wiki/Spongehttp://en.wikipedia.org/wiki/Placozoahttp://en.wikipedia.org/wiki/Cladehttp://en.wikipedia.org/wiki/Animalhttp://en.wikipedia.org/wiki/Spongehttp://en.wikipedia.org/wiki/Placozoa
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    Major differences between Protostomes and Deuterostomes

    Protostomes: blastospore develops into mouth and the second opening forms the anus

    Early cell division: cells specialize, determined cleavageCoelom formation: schizocoelous-coelom originates as a split within a bud of

    mesodermal tissue at time of gastrulation

    Deuterostomes: blastospore develops into the anus and the second opening forms themouth

    Early cell division: undetermined cleavage

    Coelom formation-enterocoelous: coelom originates from an out pocketingof the archenterons during gastrulation

    Phyla of Animalia

    Phylum Characteristics

    Ponifera Sponges

    Cnidaria Polyp/medusa, ex: jellyfish

    Platyhelminthes Flatworms

    Annelida Segmented worms, long tube like bodies, each segment containsimilar sets of organs for excretion, circulation, nerve control

    Mollusca Soft-bodied animals

    Arthropoda Joint legged animals, body segments, hard external cuticle, legs

    divided into moveable segments, ex: spider

    Echinodermata Starfish

    Chordata Vertebrate/invertebrate

    Sponges and their method of NutritionSponges filter their food from passing stream of water.

    The body has a single opening food enters, waste matter leaves

    A sponge is long and cylindrical with an opening in the middle.They have no tissue or organs.

    Unique characteristics of Cnidarians

    These organisms are of radical symmetry

    Lack a mesoderm

    Body is a digestive sac that can be one of two types

    Use tentacles to capture and eat prey

    Body plan is a sac like with a central digestive compartment, with

    gastrovascular cavity and it has a single opening

    The two shapesPolyp (asexual): cylinder-shaped and lives attached to some surface

    Medusa: flat and roams the waters looking for food

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    Platyhelminthes

    Evolutionary Significances

    Structurally more complex

    3rd embryonic layer, mesoderm, contributes to the development of more

    complex organs and organ systems, including true muscle tissues

    Significance to Humans cause troublesome diseases in humans

    tapeworms reside in the enteron or the biliary ducts of mammals

    parasites on humans

    cause much suffering and sometimes death

    cause diseases in livestock which can cause problems for humans

    some parasite on neural system

    Parthenogenesis

    Biological reproduction that involves development of a female (rarely male) gametewithout fertilization. It occurs commonly among lower plants and invertebrate animals,

    particularly rotifers, aphids, ants, wasps, and bees. An egg produced parthenogeneticallymay be either haploid or diploid.

    Unique Structure and Function of the Pseudocoelomates

    Mesoderm discontinues with regard to endoderm but not contact withectoderm

    Provides anchor matrix of muscle fibres etc. derived from suspension of

    digestive system; fluid-filled cavity, therefore hydrostatic pressure provides

    support for overall body structure and shape of organism.

    Nematodes and Humans

    Are roundworms found in moist environments

    Trichinosis is caused by a roundworm that infects meat products (usually

    pork)

    Humans ingesting infected meat can become afflicted with this disease

    Parasites of humans

    Important role in decomposition and recycling nutrients

    Research specimens (aging in humans)

    Agricultural pests that attack roots of plants

    Body Regions of a Mollusk

    Muscular footVisceral Mass

    Mantle

    Mollusks and Annelids

    The life cycle of many marine molluscs includes a ciliated larvae called the trochophore,

    also characteristics of marine annelids and other Protostomes. But mollusks lackthe one trait that most defines annelid heritage: true segmentation.

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    AnnelidsEvolutionary significance

    Muscles work against the non-compressible coelic fluid, a hydrostatic skeleton

    Muscles can alter the shape of each segment individually because the coelom isdivided into separate compartments.

    Segmentation

    Segmentation allows for a high degree of specialization of body regions. Thisregional specialization is an evolutionary development of the body plan of

    arthropods. Easier motility

    Parapodia (pair of them) (satae) that function in locomotion (in each segment of a

    polychaete)

    Arthropoda

    Success

    Greater diversity in distribution and numbers than any other phyla ofAnimalia

    Have adapted successfully to life in water, on land, and in air

    Live on top of mountains, at great depths in the ocean and in Antarctica

    Can survive extremes of temperature, toxicity, acidity and salinity

    Characteristics for the Success

    Hard external cuticle, acts as an exoskeleton for muscle attachment

    Segmentation

    Jointed legs

    Coelomate Deuterostomes: echinoderm and chordata, mammals

    Unique Traits of Echinoderms

    Water vascular system- a network of hydraulic canals branching into

    extensions called tube feet (podia) that function in locomotion, feeding and

    gas exchange

    By expanding or contracting various chambers in the water vascular system,

    water is forced into the tube feet, extending or retracting them and allowingechinoderms to walk

    Majority benthic organisms (live on sea floor)

    Most show pentamerous radial symmetry

    Bilaterally symmetrical larvae

    Characteristics of Chordates

    Have a dorsal nerve cord, and from it, nerve braches to all parts of the body

    Have a notochord which is a rod of cartilage that runs along dorsal length of

    body

    In most vertebrates, notochord only occurs in embryo

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    Have gill slits in pharynx (throat), terrestrial vertebrates only have them in

    embryonic stage.

    Example of an invertebrate chordate: a tunicate, hagfishes- they lack a series of backbones

    in their body

    -UrochordataPaedogenesis

    Reproduction by an animal that is still in the larval or pre-adult form. Paedogenesis is a

    form of neoteny and is particularly marked in the axolotl, a larvae form of salamander,which retains its larvae features owing to a thyroid deficiency but can breed, producing

    individuals like itself. If the thyroid hormone thyroxine is given, metamorphosis occurs.

    An example is the mudguppy, which retains gills and other larval features when sexuallymature.

    Characteristics of Subphylum Vertebrata

    Series of bones that make up the back bone

    Craniates (having more complex nervous system)

    Significance of being a Tetrapod

    4 feet

    limbs that can support their weight on land and feet with digits that allow

    them to transmit muscle generated forces to the ground when they walk

    live on land

    bones of the pelvic girdle, to which the hind legs are attached, are fused to

    the backbone, permitting forces generated by the hind legs against theground to be transferred to the rest of the body.

    Amniotic Egg and the AmnioteAmniotic egg contains specialized membranes that protect the embryo (humans --> amniotic

    sac). The shell around the egg slows dehydration. Amniotes also have skin thats less

    permeable and the ability to use the cage to ventilate the lungs. This allowed tetrapods toreproduce on land.

    Major Branches of Subphylum Vertebrata

    Class Characteristics

    Aves Endothermic, feathered over much of body surface; scales on legs andfeet; bones hollow and light-weight in flying species; four chambered

    hear; well developed lungs and air sacs for efficient gas exchange

    Reptilia Have scales that create a water proof barrier, lay shelled eggs on land,ectothermic; most have three chambered hearts; internal fertilization;

    amniotic egg typically laid on land

    Mammalia Mammary glands which produce milk; hair; generally larger brain than

    other vertebrates of equal size; endothermic; subcutaneous fat; hair; most

    viviparous; suckle young with milk produced from mammary glands; fourchambered heart; most have four legs;

    Amphibia Most have moist skin that complements the lungs in gas exchange

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    Osteichthyes Bony fishes; bony endoskeleton; aquatic; ectothermic; well developed

    respiratory system, usually involving gills; possesses swim bladder;paired fins; divided into two groups: ray finned fishes (which include

    most living species) and lobe finned fishes (which include the lungfish

    and coeloacanth)

    Agnatha Jawless fishes-exclude all jaw fish (vertebrates)Cephalochordata Lancelets- larvae filter feeders; adults parasites whose circular mouth is

    lined with rasping toothlike structures; many live in both salt water and

    fresh water during the course of their lives

    Chondrichthyes Cartilaginous fish: have jaws; most live in salt water; typically several gill

    slits; tough small scales with spines; ectothermic; two chambered heart;

    male possesses structures for internal fertilization

    Kingdom Plantae

    Characteristics of all Plants apical meristems

    alternation of generations

    walled spores produced sporangia

    multicellular gametangia

    multicellular dependent embryos

    Advantage of Living on Land

    more sunlight

    more access to CO2

    more habitat more nutrients

    Tissue in Land Plants (most of them)

    The vascular tissue (includes the xylem and phloem transport materials to all parts of the

    plant

    xylem: water superhighway for the plant, transporting water throughout theplant

    the phloem: transports sugar and nutrients to the various plant structures

    Major groups of Plants

    Non-vascular plants- without vascular tissue (bryophytes: mosses)Vascular plants- with vascular tissue (angiosperms and gymnosperms)

    Reproductive Strategies

    Non-vascular plants require moist habitats because their sperms must swim throughwater to fertilize the ovule.

    Vascular plants can live in dry climate because they do not require water for fertilization.

    They can use wind and animals.

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    Ferns

    Water dependenceFern species live in a variety of habitats, from remote mountain elevations, to dry

    desert rocks faces, to bodies of water or in open fields. Some live in extremely

    acidic habitat and other live in basic ones. They are vascular plants and do notrequire a lot of water even though they lack seeds and flowers. Although their

    preferred habitat is a moist one, they can survive in dry areas as well. They do not

    entirely depend on water for reproduction.

    Major Groups that Reproduce Using Seeds

    Gymnosperms- naked seeds

    Angiosperms- flowers plants

    Significance of the Angiosperms

    Protect their seeds within the body of a fruit

    Seeds are better protected of nutrients

    Can be carried to farther places and can be displaced farther than those of

    gymnosperms

    Flowering plants

    Major Strategies for Plant pollination

    Seeds (gymnosperms + angiosperms)

    Anemophily (wind)

    Hydrophily (water)

    Biotic pollination : organisms that transport pollen

    Entomophily (insects)

    Zoophily (vertebrates, ex: birds and bats)

    Development of Fruit

    Various fruit adaptations help disperse seeds

    Dandelions: fruit function like propellers--> wind

    Coconuts: dispersal by water

    Burrs: cling to animal for dispersal

    Edible fruits: animal eats --> deposit seeds with feces--> acts like fertilizer

    It helped plants to be dispersed to a larger area and become more wide spread. Their seeds

    are also protected.

    Virus

    Living Things?

    Not considered living things

    Have no cellular structure

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    No cytoplasm, organelles or cell membranes

    Do not carry out respiration or any other common life processes

    Consist of little more than strands of RNA or DNA surrounded by a

    protective protein coat called a capsid

    Parasite cells; capsid protects the virus from attacks by the host cell

    enzymes.

    Structure of Virus

    Tobacco mosaic virus : helical capsid with the overall shape of a rigid rod

    Adenoviruses: a polyhedral capsid with a glycoprotein spike at each vertex

    Influenza viruses: outer envelope studded with glycoprotein spikes. Thegenome consists of eight different RNA molecules, each wrapped in a

    helical capsid

    Bacteriophage T4: like T-Even phages, has a complex capsid consistingof a polyhedral head and a tail apparatus

    Depending on the virus, the capsid may be rod-shaped, polyhedral, or more complex shape

    Characteristics that led to a Classification of Viruses into 21 Groups

    Life Cycle

    Lytic cycle1. Attachment

    2. Entry: the virus inject its nucleic acid into the host cell

    3. Replication: hosts metabolism replicates the viral RNA or DNA

    4. Assembly: new virus particles are assembled5. Lysis and Released: host cell breaks open and release new viruses

    Life Cycle of Provirus

    Enzyme called reverse transcriptase causes the host cell to copy the

    viral RNA into DNA

    Viral genome can enter the chromosomes of the host cell and can be

    copied whent the cell divides

    Virus is now called a provirus

    When host cell undergoes mitosis, it replicates the provirus

    Process can continue for years and does not kill host cell

    However, provirus can separate from host chromosomes and

    complete the lytic cycle

    Why Viruses are Important for Humans

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    Biotechnology

    The gene is spliced into the genome of a virus

    The virus gets copied with the gene through host cell

    Helps genetic engineers

    Cancer Research

    Can possibly cure cancer Still under research

    People who had cancer and caught a severe virus and survived are cancer

    free

    Origin of Viruses

    Scientist believe that they evolved after the first cells

    They cannot replicate without host cells

    Probably originated as fragments of nucleic acid that escaped their original

    acid

    Survived by becoming parasites of same or similar types of cells

    Taxanomy

    Taxa

    Domain

    Kingdom

    Phylum

    Class

    Order Family

    Genus

    Species

    Binomial Nomenclature

    Two-word name for each species

    Latinised and first word always describe the genus of the species

    First letter of the first word is always capitalized

    Second word is a particular name given to the species that no other species

    have within the same genus

    Common names are misleading; same name can be given to two completely

    different species

    With scientific naming, no two species have the same name

    Sources of Genetic Variety

    Mutation

    Meiosis (sexual reproduction)

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    Meiosis allows different gene combinations

    Crossover occurs where homologous chromosomes have the potential toexchange bits of DNA

    Two haploid cells fuse, create a cell with a brand new set of DNA that has

    never been formed before

    Carry adaptations to survive their environment

    Determining Relatedness

    Fossils: ancestral characteristics that may have been lost over timeBiochemical evidence: similar protein structures; compare different proteins of organisms;

    more similar, more closely related

    DNA evidence: compare introns; the bigger the difference, the longer ago the two animalsbranched from each other

    Homologous organs: similar developmental origins, different adult functions

    Vestigial Organs: structures or organs that seem to serve no useful function

    Cladistics and Cladograms Cladistics is a classification scheme based on phylogeny

    System based on the idea that each group of related species has one

    common ancestor

    Organisms retain some ancestral characteristics as they evolve and diverge

    from one common ancestor

    Cladogram is a branching diagram that resembles a phylogenic tree, but can

    be used to test alternative hypotheses

    One common ancestor, branches are drawn to represent differentevolutionary processes that took place and the organisms that evolution

    created

    More recent the group split, more related they are

    Monophyletic, paraphyletic, polyphyletic (Look on Wikipedia for more

    information)