Speeder Gel Documentation System

THREE YEAR QUALITY GUARANTEE!

Application A complete range of gel doc systems to support white light, UV light applications for 1D and 2D gel analysis. Gel documentation is at the fore-front of molecular biology research. Our high quality bio-imaging systems deliver excellent results in many types of applications and condi-tions: Colony Counting Plants Western Blots Northern Blots Southern Blots Dot Blots DNA RNA Microtiter Plates 1D Gels

Speeder1200 Gel Documenta-tion System

Configuration 1) 12 mega high resolution scientific

grade digital CCD camera, with time integral function

2) Computerized zoom lens 3) Filter: UV/IR 4) Controller: computer software 5) Darkroom with double door sealing 6) Working table:

312nm UV transilluminator (standard, for nucleic acid application) Part No.: UV312*2520 25×20cm transilluminating area, or

white light table

(optional, for protein application) Part No.: WL2520 25x 20cm illuminating area

7) Image capture software: BIO-CAPT 8) Analysis software: BIO-1D 9) 254nm 365nm UV side light (optional) 10) UV protection shield: UPS (optional) 11) Gel cutting ruler: GCR (optional) 12) Computer (optional) 13) Analysis software: BIO-2D (optional)

Gel Documentation System (A Series) Hardware Features 1) Automatically controlled by computerized software: on-off of

UV/white light, zoom lens, acquisition & analysis of images 2) High resolution scientific grade digital CCD camera 3) Pull-push working table 4) Unique fan and airtight air-flue design 5) Diverse light sources are available, with side lights 90 degree

freely bent and rotated 6) Double door sealing system

BIO-1D Analysis software Functional Features

1) Microsoft application software features (cut, paste, copy...) 2) Lane precision set-up 3) Manual or automatic band detection 4) Molecular weight calculation 5) Rate of Flow (RF) calculation 6) Phylogenetic tree analysis 7) Optical Density (OD) calculation 8) Microtiter plate analysis 9) Colony counting 10) Image enhancement function 11) Print design 12) Report design 13) Diverse output formats 14) Results summary 15) Other functions

Gel Documentation System Speeder

Features of Components

The Main components consist of:

1. CCD Camera (for Gel Imaging application)

Scientific grade digital CCD camera, with time integral function.

Auto Focus, image can be got within seconds

Resolution:

Speeder1200 4000×3000 Speeder800 3264×2448 Speeder500 2560×1920

CCD size 1/2 inch Time of exposure 1-500 ms Dynamic range 74db Pixel 5.2um-5.2um Lens (Manual or Motorized) 8.1-51mm Digital zoom Real time x 6 Sensitivity 0.001 LUX Signal to noise ratio >62dB

2. Cooled CCD Camera (For Chemiluminescence application)

Scientific grade digital CCD camera, with time integral function.

Imaging Resolution 1560×1200 CCD Temperature -30℃

3. Darkroom Including: A metallic case and door with antioxidant coating A fixed camera support A UV safety switch A tray with orbital slides for UV transilluminator and white light table. Size: 540H×320D×435W mm

4. UV Transilluminator 312nm UV Transilluminator, two models are available: UV2020 20×20cm Transilluminating Area UV2520 25×20cm Transilluminating Area

5. White light Table (optional) For application of protein gel’s observation or photography, autoradiogram or microtiter plates. Illuminating area: 25×20cm

6. UV side light (optional) Reflected UV Light: 254nm and 365nm

7. Software Image Capture Software: Bio-Capt Analysis Software Bio-1D (for 1D gel analysis)

Five kinds of light sources avail-able: Transmission Light: UV312nm, white light Reflected light: UV254nm, UV365nm, Overhead white light.

Transilluminator

UV Transilluminator & White Light Table

In genetic research field (especially nucleic acid and protein research application), agarose or polyacryla-mide gels are usually used to separate DNA or RNA fragments. To locate these fragments, DNA or RNA bands have to be stained with ethidium bromide or acridine orange, and then excited by ultraviolet light. We place gel on the surface of working table, transilluminating it by UV or white light, then we obtain a visible fluorescence of the band.

Transilluminator Specification

Model Transilluminating Area Wave-

UV312*2020 20cm × 20cm 312nm

UV312*2520 25cm × 20cm 312nm

UV312*3050 30cm × 50cm 312nm

UV254*2020 20cm × 20cm 254nm

UV254*2520 25cm × 20cm 254nm

UV254*3050 30cm × 50cm 254nm

UV365*2020 20cm × 20cm 365nm

UV365*2520 25cm × 20cm 365nm

UV365*3050 30cm × 50cm 365nm

WL2520 25cm × 20cm white light

UV254L 20cm × 5cm 254nm

UV365R 20cm × 5cm 365nm

Stepless UV Light Intensity Adjus-ment

UV intensity is adjustable, which could be reduced properly to reinforce protection for samples (only for T controller).

Air Cooling Fan

Two air cooling fans prevent gels from any dam-age caused by overheating.

Long-life Filter

GOLD SIM transilluminator is equipped with a long-life filter which provides a high level of UV trans-mission for all over the working area.

Antioxidant coated metal frame

Easy maintenance.

White Light Table

For application of protein gels observation or pho-tography, autoradiogram or microtiter plates.

UV transilluminator UV312*2020, 20×20cm Transilluminating Area UV312*2520, 25×20cm Transilluminating Area

White light Table WL2520 25×20cm Illuminating area

Bio-Capt Image Capture Software

1. Images capture and save as different formats

(Tiff, Bitmap, Jpeg, Pict, pcx, Gif, Targa...)

2. Images display in menu and marking

3. Images processing: rotation, inversion, bright-

ness and contrast adjustment.

4. Diverse output formats. Bio-Capt Capture soft-

ware can work together with lots of application

software.

5. Exposure time is adjustable.

6. High image capturing sensitivity.

BIO-1D Analysis Software

1. Microsoft application software features (cut, paste, copy, rotate...)

2. Lane precision set-up 3. Manual or automatic band detection 4. Molecular weight calculation 5. Rate of Flow (RF) calculation 6. Phylogenetic tree analysis 7. Optical Density (OD) calculation 8. Microtiter plate analysis 9. Colony counting 10. Image enhancement function 11. Print design 12. Report design 13. Diverse output formats 14. Results summary 15. Other functions

Bio-Capt Software

Lane Precision Set-up

Manual & Automatic Band Detection

Single or all Lanes Correction function: Lane Direction, gap and slope are adjust-able. Using up or down arrow to change the value of parameters, or directly enter a specific value. The range of slope: -100~100°

Bio-1D Software

Vertical and horizontal lane adjustment

Vertical and horizontal lane slope adjustment

Visually viewing all selected bands, easy for verifica-tion, comparison, calculation of molecular weight (MW), Rate of Flow (RF), Optical Density (OD), etc.

You can add or delete a detection marker following the instruction of shown graph, which creates more pre-cise result by preventing any missed or wrongly added markers. Also we can add a new marker on the graph just by moving cursor onto a position between two peaks, then click when cursor becomes a double headed ar-row.

Bio-1D Software

Parameters adjustment: slope, sensitivity. Migration correction: suitable for three or more markers.

Molecular Weight (MW) Calculation

Using molecular weight of standard lane to calculate that of other lanes. Gel images and result are displayable.

Marker Editor enables you to enter a specific value, then press Enter to confirm. All values are displayed in a descending order.

Graphic illustration of MW calculation result. Lanes can be compared with a user-defined standard lane.

Bio-1D Software

Rate of Flow (RF) Calculation

RF means the ratio of flow distance between sam-ple and buffer.

Phylogenetic Tree Analysis

Genetic Relationship is shown as a ma-trix.

Phylogenetic Tree

Define different colors for values in matrix to get a vivid visual effect.

Matching function is similar to pholygenetic tree function, but it can get phylogenetic tree graph using selected band as contrast.

Bio-1D Software

Optical Density (OD) Calculation

Volumes

Units may be changed to ng, mg, %, etc. You can calculate relative content and absolute con-tent at different position of each band.

Get more precise result by activat-ing crest-magnify button.

Calculate volume of all lanes according to the volume of detected lane.

Microtiter Plate Analysis

Compatible with any microtitier plates, easy for analysis.

Display the outline and results of different bands in the same window to facilitate comparison.

Bio-1D Software

Qualitative and quantitative analysis of colony. All necessary information is listed.

Colony counting is based on flexible detec-tion parameters. Parameter modification is to add or filter colonies depending on their specific characteristics.

Image preview helps you easily re-trieve your images. All images in the same directory are displayed in small viewable format.

Colony Counting

Image Enhancement

Gray value is adjustable.

Print Design

The report including different selected data and chart is reorganized in a layout defined by operator.

All selected data could be displayed in the same file. What you need to do is just to select your desired data and drag it into the report layout.

Bio-1D Software

Background adjustment: Grey level is adjustable. This function is to outstand and enhance the image feature.

Background Inversion is suitable for print-ing electrophoresis images. You may reverse image so as to outstand image feature and also save your printer consumables.

Output Format

User-friendly spreadsheet includes useful features such as ratios, data import & ex-port function.

Images could be output as format of tif, bmp, png, jpg. Output formats: clipboard, Excel, PDF, data-base, etc. 3D image observation and output is available.

Results Summary

Result list displays all calculated results. “comment“ under the “edit” menu displays all calcu-lated results, easy for editing and report output.

Bio-1D Software

Bio-2D is an unique software especially used for 2D gel analysis application. Fast and accurate analysis is done just through a few clicks.

Calculation Function

1. Background subtraction by overlap. 2. Outline recognition by local comparison. 3. Manually define spots. 4. Detecting volume, gravity center, maximum intensity,

height, circularity of every spot. 5. Calculating MW, isoelectric point of each spot as per

user’s definition 6. Correcting distortion image by trigonometry. 7. Correcting intensity between images by one or more refer-

ence points. 8. Screen display of matching and non-matching spots. 9. Spots movement observation.

Database Function

1. Variable database, 10 levels including 999 sublevels. 2. Data storage of all detected points of image.

Spot Characteristic Definition

Date is saved in a database with mass storage capacity. Intuitive tools makes database management very simple.

MW marker is included in the sample type, as well as PL scale. Specific MW and PL value are assigned to spots according to the proportion de-fined on the image.

Spot detection methods includes a threshold close to accurate spot defini-

The comparison is expressed either in volume or in gravity center movement of the matching spots. The comparison is to select matching spots or values of all samples.

Each spot is marked and defined its gravity. Each spot could be in directly conjunction with to relative analytical

Spot Matching

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Adjustment is often used to correct dis-torted images so as to make compari-

Bio-2D Software

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Order Information

1. 5.0 mega, 8.0 mega and 12.0 mega high resolution scientific grade digital CCD camera, with time inte-

gral function.

2. Computerized zoom lens.

3. Filter: UV/IR.

4. Controller: computerized software, auto focus

5. Darkroom with double door sealing system.

6. Working table: 312nm UV table (suitable for nucleic acid experiment).

UV312*2520: 25×20cm filter transilluminating area or

White light table (suitable for protein experiment, Model: WL2520, optional).

6. 254nm 365nm UV side light.

(optional for Bio-Best 140A, standard configuration for Bio-Best 200A and Bio-Best 300A).

6. UV protection shield. Model: UPS (optional).

7. Gel cutting ruler. Model: GCR (optional).

8. Computer (optional).

9. Image capture software. Model: BIO-CAPT.

10. Analysis software. Model: BIO-1D (for 1D Gel application); Model: BIO-2D (for 2D Gel application)

Speeder Gel Documentation System

Model Resolution Pixel size Mode of Zoom

Mode of Switch

Bit Depth CCD temp. Size of UV Fil-ter

Speeder500 2560×1920 5.0 mega Cybernate Cybernate 16 bits Normal 25×20

Speeder800 3264×2448 8.0 mega Cybernate Cybernate 16 bits Normal 25x20

Speeder 1200

4000×3000 12.0 mega

Cybernate Cybernate 16 bits Normal 25x20

Notes: Speeder series is auto focus gel doc, image can be got within seconds .

Specification

SIM INTERNATIONAL GROUP is a multinational corporation. The headquarter is located in Califor-nia of USA which specializes in research and development of automatic control system. We have branch in Delaware Newark specializing in research, development and manufacture of bioscience and life science instruments.

SIM entered into China market since the year of 1995 and established China branch in the year of 1999. In the year of 2004, to optimize global resources, SIM transferred the laboratory instruments product line from USA to China and established production plant in Beijing of China-GOLD SIM (BEIJING) INTERNATIONAL CO. LTD.

Now, we have branches handle our products in European countries, in the United States and in the Asia-Pacific region. We are present worldwide via authorized representatives.

SIM PRODUCT LINE

- Freeze Dryer - Gel Imaging documentation System - Incubator - Hybridization Oven - Bio-Safety Cabinet - Rotating Incubator - Pipettor

SIM USA INC.

SIM INTERNATIONAL GROUP CO. LTD. Add: 113 Barksdale Professional Center, Newark, DE19711, USA. Email: asales@sim-lab.com

SIM USA is the research and development center since its establishment. . Now, the new R&D focus is placed on auto-matic control system.

SIM UK CORPORATION. Add: De Castro Street Wickhams Cay I Road, Town Tortolam, UK. Email: esales@sim-lab.com

SIM UK CORPORATION is another branch of SIM INTERNATIONAL GROUP located in U.K.

GOLD SIM (BEIJING) INTERNATIONAL CO., LTD. Address: Room B1405, Gongshanlian Mansion, Fufeng Road No. 4, Science City, Fengtai District, Beijing 100070, P. R. China. Tel: 86 10 63899566 Fax: 86 10 83811149 Email: csales@sim-lab.com website: www.sim-lab.com

SIM entered into China market in the year of 1995, established China branch in 1999 and established laboratory instru-ments production plant - GOLD SIM (Beijing) International Co., Ltd in the year of 2004.

Focused and Steadfast Our research, development and production are located in Beijing of China in a space of 9000m2. All GOLD SIM employees are currently working on fulfilling our client’s wishes. Their satisfaction is our measure of suc-cess.

Open and Individual Our comprehensive accessories also contribute to our customer-friendliness. They especially make our instruments very adaptable and their operation economical. We are always open for new wishes and develop customized solutions. Highly specialized in construction and produc-tion, we respond both quickly and individually.

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