Sogesta Center, Urbino, ItalyJune 29-July 5,1980

UNIVERS'TATSB'BLiOTHEKHANNOVER

TECHNiSCHEINFORMATIONSBIBLIOTHEK

Patron Editore - Bologna

CONTENTS

OXIDO-REDUCTION CHAINS IN BACTERIA, MITOCHONDRIA ANDCHLOROPLASTS

S I B. ChanceTheoxycytochromeoxidasereaction pag. 3

S I R.A. CapaldiStructure and function ofcytochrome c oxidase » 5

S I G. SchatzComposition and biogenesis of the mitochondrial oxidative phospho-rylation system » 7

S I A. Baccarini Melandri, N. Gabellini, B.A. Melandri, E. Hurt, G.Hauska, W.A. Rutherford, A.R. CroftsCyclic photosynthetic electron flow in chromatophores: the role ofspecializedquinone molecules » 11

S I H.T. Witt, R. Tiemann, D. Di FioriA. On the nature of the transmembrane electric potential differencein the steady stateB. On the behavior of fluid and rigid thylakoid layers spread at inter-faces and large plates » 15

S I G. Hauska, G. Orlich, D. Samoray, E. Hurt, P.V. SaneReconstitution of photosynthetic energy conservation with photosy-stem I - Reaction centers from chloroplasts » 17

SI S. Papa, F. Guerrieri, M. LorussoThe proton translocating function of the cytochrome system of mito-chondria » 19

RT I A. Azzi, R.P. Casey, M. ThelenThe proton pump of cytochrome c oxidase: inhibition by carbodiimi-des pag. 21

RT I C.R. Hackenbrock, H. Schneider, J.J. Lemasters, M. HochliDiffusional activity of redox components related to electron transferin the mitochondrial energy transducing membrane » 23

RT 1 G. von Jagow, W.D. EngelA hypothetic model for cytochrome b of ubiquinol: cytochrome c re-ductase (EC 1.10.2.2) as a proton translocator » 25

RT I H. Weiss, P. Wingfield, K. Leonard, F. Winkler, S. Perkins, A. Mil-lerStructure of ubiquinone: cytochrome c reductase from Neurosporamitochondria » 27

RT HI A.R. Crofts, J.R. Bowyer, S. Meinhardt, A.W. RutherfordPhotosynthetic electron transport in Rps. sphaeroides and Rps. capsu-lata » 29

RT III A.L. Lehninger, B. Reynafarje, A. Alexandre, A. Beavis, P. Davies,C. Mahle, A. VillaloboThe proton stoichiometry of mitochondrial electron transport » 31

C I M.C.W. Evans, A.W. RutherfordDetermination of the redox properties of the primary and secondaryelectron acceptor in the reaction centre o/Rhodopseudomonas sphae-roides » 33

C I M. Snozzi, S. Bodmer, R. BachofenStudies on the quinones in the membrane of the photosynthetic bacte-rium Rhodospirillum rubrum » 35

C I B.L. TrumpowerResolution and reconstitution of the iron-sulfur protein of the cytoch-rome b-c, segment of the mitochondrial respiratory chain » 37

CI S.P.J. AlbrachtThe prosthetic groups in succinate dehydrogenase » 39

C I G. Buse, G.J. Steffens, G.C.M. Steffens, R. Sacher, M. ErdwegPrimary structure and function of cytochrome c oxidase subunits » 41

C I M. Gutman, D. Huppert, E. Pines, E. NachalielProbing of macromolecular surface by laser induced pH jump » 43

C I A. KrOgerGeneration of the electrochemical proton potential byfumarate reduc-tion in Vibrio succinogenes » 45

C I G. Akoyunoglou, S. TsakirisIndependent growth of the photosystem I and II units pag. 47

P I I.M. Moller, S.P. Johnston, J.M. PalmerThe effect of cations on exogenous NADH oxidation - General chargescreening anda specific requirement for Ca1* » 49

P I S.R. Earle, R.R. FisherThe stoichiometry of proton translocation coupled to transhydrogena-tion in reconstituted vesicles » 51

P I J.T. Duniec, S.W. ThorneTransmembrane surface charge asymmetry and the electron transportin chloroplasts » 53

P I N.V. KarapetyanElectron transport in photosystem I of subchloroplast fragments » 55

P I W. Oettmeier, U. JohanningmeierBinding of DCMU and DBMIB-type inhibitors to the chloroplastmembrane » 57

P I P.R. Rich, D.S. BendallA model for electron transfer from quinol to the be complex » 59

P I M.T. Fernandes Moreira, P.R. Rich, D.S. BendallA method for demonstrating the involvement of a mobile quinonepool in an electron transport chain by the use of loosely bound inhibi-tors » 61

P I A.A. Konstantinov, Yu. Kamensky, M. Ksenzenko, S. SurkovStudies on the Q-cycle in the mitochondrial respiratory chain » 63

P I A. De Santis, E. Bertoli, B.A. Melandri, A. Baccarini MelandriThe reconstitution of oxidative phosphorylation in mitochondria froma ubiquinone-deflcient mutant of yeast » 65

P I M. Degli Esposti, E. Bertoli, G. Parenti-Castelli, C. Casali, R. Fato,G. LenazEffect of exogenous ubiquinones on mitochondrial membranes » 67

P I CA. Boicelli, G. Sartor, E. Casali, L. MasottiQ homologues interactions with lipids and water in model membranes. » 69

P I T. Ohnishi, H. BlumLocation of the Rieske iron-sulfur cluster in the mitochondrial mem-brane » 71

P I V.Yu. Artzatbanov, A.A. KonstantinovEffect ofantimycin on the EJpH - dependence ofb cytochromes inbeef heart submitochondrial particles » 73

P I H.R. Bosshard, R. Rieder, M. Zurrer, B. Waldmeyer, R. BechtoldIs there a common recognition site for cytochrome c on structurallydissimilar hemoproteins? pag. 75

P I P.A.M. Michels, B.A. HaddockCytochrome c-deftcient mutants o/Rhodopseudomonas capsulata » 77

P I K. Dancheva, M. Mitovska, G. Kossekova, B. AtanasovEffect of chemically modified derivatives of cytochrome c on electrontransfer » 79

P I M. Denis, E. Neau, I. Agalidis, P. PajotPotentiometric resolution of cytochromes c and c, in yeast mitochon-dria » 81

P I K.R. Leonard, T. Arad, P. Wingfield, H. WeissThree dimensional image reconstruction of cytochrome c reductasefrom Neurospora mitochondria » 83

P I C. Montecucco, H. Gutweniger, R. Bisson, F. Dabbeni-Sala, A. Pi-totti, P. Zaccolin, A. Mascia, M. Santato, P. ArslanPhotolabelling of the hydrophobic sector of mitochondrial oligomycinsensitive ATPase and succinate cyt c reductase » 85

P I R. Rieder, H.R. BosshardComparison of the binding sites on cytochrome c for cytochrome coxidase, cytochrome c reductase, and cytochrome c, .-. » 87

P I B. LudwigA two-subunit cytochrome c oxidase from Paiacoccmdenitzificans.... » 89

P I B. Kadenbach, P. MerleThe subunit composition of mammalian cytochrome c oxidase » 91

P I B.H. Ariano, A. AzziThe motion of cytochrome c oxidase, inlayed in phospholipid vesiclesis fast and' anisotropic » 93

P I G.C.M. Steffens, L. Prochaska, R.A. Capaldi"C-DCCD and "S-NAP-Taurine binding sites on cytochrome oxidase » 95

P I E. Sigel, E. CarafoliQuantitative analysis of the charge stoichiometry of reconstituted beefheart cytochrome c oxidase » 97

P I M. Lorusso, D. Boffoli, F. Capuano, N. Capitanio, V. Pace, S. PapaMechanism of A/ZH* generation by cytochrome c oxidase » 99

P I R.P. Casey, C. Broger, A. AzziNCCD - A spin label probe of the cytochrome c oxidase proton pump. » 101

P I R. Bisson, R. A. CapaldiCytochrome c binding sites in cytochrome c oxidase and cytochrome cperoxidase pag. 103

P I A. Alexandre, A.L. LehningerProton ejection coupled to ferrocytochrome c oxidase by rat liver mi-toplasts » 105

P I R.K. Poole, B. ChanceIntermediates in the reaction with oxygen of cytochrome d in oxygen-limitedEscherichia coli » 107

P I G. DucetMembrane potential changes seen by rapid scan spectrometry » 109

P I A.A. Jasaitis, Z.J. KrivickieneKinetic characteristics and physiological significance of the intermem-braneelectron transfer in mitochondria » 111

P I R. KiehlThe effect of the lipophilic thiol reagent NSPMon ox. phos. and resp.chain » 113

ATP SYNTHESIS IN BACTERIA, MITOCHONDRIA AND CHLOROPLASTS

S II L.M. Amzel, P. Narayanan, P.L. Pedersen, J. HullihenStructure of FrATPases » 117

S II W. Sebald, H.U. Schairer, P. Friedl, J. HoppeStructure and genetics of the A TPase proteolipid » 119

S II N. NelsonSubunit - structure, function and biogenesis of proton ATPase com-plexes » 121

S II C. Carlsson, L. ErnsterStudies on the reaction mechanism of mitochondrial A TPase » 123

S II G. Klein, M. Satre, AJC. Dianoux, P.V. VignaisInteraction of mitochondrial ATPase with its natural inhibitor radio-actively labeled » 125

S II H. Strotmann, J. SchumannFunctional properties of chloroplast A TP synthetase » 127

RT II B.A. Melandri, G. Venturoli, A. Baccarini Melandri, J.B. JacksonPre-steady state events of bacterial photophosphorylation: thresholdeffect by the phosphate potential, photosynthetic control andATP/2er ratios • » 129

RT II L.N.M. Duysens, H.N. van der Wai, R. van GrondelleIs a proton membrane potential between inner and outer space ofchromatophores necessary for photosynthetic phosphorylation ? pag. 131

RT 11 P.D. BoyerThe binding-change mechanism for A TP synthesis and its relation toproton translocation » 133

RT II J.B. Jackson, N.P.J. CottonEnergy coupling in intact cells of Rhodopseudomonas capsulata aftershort flashes » 135

RT II T. Graan, D.R. OrtThe dependence of the initiation of photophosphorylation in isolatedchloroplasts on both the electrical and chemical potential of the pro-ton gradient » 137

RT IV K. van Dam, H.V. WesterhoffThe use of thermodynamic equations describing energy transduction .. » 139

RT IV H. RottenbergEnergy conversion and the proton current pathways in mitochondrialmembranes » 141

RT .V J.W. StuckiOptimization of mitochondrial energy conversion » 143

RT IV D. WalzNon-equilibrium thermodynamic treatment of mitochondrial proces-ses » 145

C II G. Schafer, G. Onur, M. SchlegelNucleotide analogs as probes in oxidative phosphorylation: observa-tion of cooperativity » 147

C II D.C. Gautheron, C. GodinotEssential thiol groups in oxidative phosphorylation » 149

C II E. Bauerlein, H. TraschInteraction between alkyl chains with and without functional groupsand beef heart mitochondria » 151

C II P.L. Pedersen, J. Hullihen, J. Wehrle, K. Schwerzmann, N. CintronH*-A TPase of rat liver mitochondria - Progress in understanding howit functions and how it may be regulated » 153

C II I.A. KozlovThe mechanism of transformation of the energy ofA^n- — ATP on themitochondrial membrane » 157

C II B.T. Storey, D. Scott, C.P. Leelntramembrane and extramembrane H* movements in submitochon-drial particles from skeletal muscle: membrane Bohr FT in energy cou-pling pag. 159

C II J.E.G. McCarthy, P.R. Alefounder, S.J. FergusonMechanistic aspects of aerobic and anaerobic oxidative phosphoryla-tion in Paracoccus denitrificans » 161

C II E. Munoz, J.M. Andreu, J. Carreira, V. Larraga, A. Marquet, F.Mollinedo, M. Nieto, J.P. PivelPolymorphism and conformatiohal dynamics of energy transducingFi-ATPase from a bacterial membrane system » 163

P II H.W. Miiller, M. Baltscheffsky, K. DoseGradually disintegrated A TPase complexes from a photosyntheticbacterium: subunit composition, kinetic properties, reconstitution,and energy-dependent reactions in proteoliposomes * » 165

P II L. SlootenATPase activity and nucleotide exchange in Rhodospirillum rubrumchromatophores » 167

P II J. Lunardi, P.V. VignaisArylazido - nucleotide analogues as photoaffinity labels for the E.coliadenosine triphosphatase » 169

P II M. Satre, G. ZaccaiSmall - angle neutron scattering o/Escherichia coli BFx-ATPase » 171

P II E. Wachter, R. Schmid, G. Deckers, K. AltendorfThe dicyclohexylcarbodiimide-reactive protein from E.coli - Molecu-lar implications of aminoacid replacements and chemical modifica-tions » 173

P II H.J. Schafer, P. Scheurich, G. Rathgeber, K. DosePhotoaffinity labeling, fluorescent photoaffinity labeling, and pho-toaffinity cross-linking of F,-ATPasefromWlicrococaislvitew » 175

P II M.C. Bohler, M. Wolf, A. Binder, R. BachofenThe coupling factor AF, in membranes of the thermophilic cyanobac-terium Mastigocladus laminosus » 177

P II E. Stutterheim, M.A.C. Henneke, J.A. BerdenThe subunit stoicheiometry of yeast Ft: binding ofaurovertin and ra-dioactive labeling » 179

P II R.J. Berzborn, L. Hein-HitpassTitrations concerning If flux from electron transport chains to the en-zyme of photophosphorylation CFt » 181

— AV111 —

P II C. VinklerUncouplers and inhibition of electron transport have opposite effectson the apparent Km of ADP forphotophosphorylation pag. 183

P II H.M. Younis, N.A. Morjana, N.M. BakryLow molecular weight chloroplast protein, a natural inhibitor ofchlo-roplast coupling factor 1 adenosine triphosphatase » 185

P II R.J. Wagenvoord, W.P.A. van Vliet, A. Kemp, E.C. SlaterBinding sites of 8-azido-adenosine analogs on Fi » 187

P II M.D. Partis, D.G. Griffiths, R.B. BeecheyA transmembrane location of the proton translocating A TPase » 189

P II B. Norling, E. Glaser, L. ErnsterThe dicyclohexylcarbodiimide-binding protein of the mitochondrialATPase complex from beef heart » 191

P II J.B. Hughes, S. Joshi, D.R. SanadiOn the role of mitochondrial coupling factor B in energy-linked reac-tions » 193

P II F. Dabbeni-Sala, A. Pitotti, A. BruniResolution of mitochondrial ATPase complex by dinonanoylphospha-tidylcholine » 195

P II C. Godinot, D.C. Gautheron, Y.M. Galante, Y. HatefiLocation ofthiols essential in theATP-P, exchange of complex V » 197

P II Y.M. Galante, S.Y. Wong, Y. HatefiInteraction of complex V (ATP synthetase complex) with the ATPaseinhibitor protein » 199

P II D. Griffiths, M.D. Partis, A. Williams, R.B. BeecheySynthesis and biological properties of some novel crosslinking rea-gents » 201

P II M.J. Gresser, G. Rosen, P.D. BoyerBound catalytic nucleotides in ATP synthesis and degradation » 203

P II W.E. Kohlbrenner, P.D. BoyerOne type of catalytic site will account for synthesis and hydrolysis ofATP by mitochondria and chloroplasts » 205

P II F. Guerrieri, S. PapaMolecular mechanism of proton conduction by the mitochondrial fT-ATPase » 207

P II G.D.V. van Rossum, S.C. Kapoor, T. Galeotti, G. Palombini, M.L.EboliInhibition of oxidative phosphorylation by SKF 525A in intact cells

and isolated mitochondria pag. 209

P II H. Trasch, E. BauerleinAcetylenedicarboxylic acid dimethylester, a new inhibitor of oxidativephosphorylation » 211

P II C.G. Jackson, R.B. Beechey, P.J.F. Henderson, P.E. LinnettThe efrapeptins, a family of potent inhibitors of the mitochondrialATPase » 213

P II P.N. Lowe, P.E. Linnett, H. Baum, R.B. BeecheyMgATP - inducedinhibition of the mitochondrial ATPase » 215

P II H.V. Westerhoff, J.C. Arents, A.L.M. Simonetti, P.C. de Jonge,J.A. Bode, K. van DamMechanistic conclusions from a non equilibrium thermodynamic ana-lysis of bioenergetic experiments » 217

P II J.J. LemastersKinetic control and thermodynamics of oxidative phosphorylation » 219

P II W. Kunz, G. BOhme, P. Schonfeld, U. Kuster, R. BohnensackMitochondrial phosphorylation potential, hydrogen pressure and rateof ATP formation » 221

P II A.B. Hope, D.A. Walker, P. Horton, D. RansonPhotophosphorylation in chtoroplasts with varied proton motive force » 223

P II K.J. Hellingwerf, K. Nicolay, R. Kaptein, J. Lolkema, W.N. KoningsThe proton motive force in Rhodopseudomonas sphaeroides » 225

P II L. Wojtczak, K.S. Famulski, A. Dygas, M.J. NaleczChanges of the surface potential of the inner mitochondrial membraneupon energization » 227

P II G. Girault, J.M. GalmicheEnergization of the thylakoid membranes by ATP as measured withoxonol VI in spinach chloroplasts » 229

P II R. Casadio, G. Venturoli, B.A. MelandriTransmembrane potential difference in chromatophores of photosyn-thetic bacteria: measurements with a membrane ion selective electrode » 231

P II M.C. Sorgato, D. Branca, S.J. FergusonRelationship between the rate of A TP synthesis and a) rate of respira-tion, b) magnitude of the protonmotive force in submitochondrialparticles » 233

P II D. Branca, S.J. Ferguson, M.C. SorgatoThe H*/A TP phenomenological coefficient in submitochondrial parti-cles: its dependance on the composition of the reaction mixtures » 235

P II M. Pozzan, D. Pietrobon, G.F. AzzoneThe relationship between phosphate potential and proton motive forcein static head rat liver mitochondria pag. 237

P II D. Pietrobon, G.F. AzzoneThe relation between H* pumps and leaks in rat liver mitochondria » 239

P 11 F. Di Virgilio, G.F. AzzoneHVsite, charge/site and ATP/site ratios in rat liver mitochondria » 241

P II M. Schuermann, W.G. HansteinUncoupling of energy-dependent transhydrogenation: unexpected de-pendence on the mode of energization » 243

P II F. FreundProtons and defect protons - A new concept for vectorial proton tran-sport in membranes » 245

TRANSPORT OF IONS AND METABOLITES IN BACTERIA, MITOCHONDRIAAND CHLOROPLASTS

S III S.R. CaplanThermodynamics of active proton transport » 249

S III T.E. Conover, G.F. AzzonePrimary events in energy transduction: the rise of electrical field andits relation to H* extrusion » 251

S HI M. WikstromMechanism of proton translocation by mammalian cytochrome oxida-se » 253

S HI Yu.A. OvchinnikovMechanism of ion transport through biological membranes: ionopho-res and ion channels , » 255

S III W.A. HamiltonEnergy coupling in microbial secondary ion-linked solute transport sy-stems » 257

S III E. CarafoliInflux and efflux of calcium in isolated mitochondria: recent develo-pements » 259

C HI L. GriniusIon gradient-driven transport of DNA » 261

C HI I.R. Booth, S. Ahmed, W.A. HamiltonEnergy coupling to lactose transport in Escherichia coli » 263

- X X I -

C III G.L. Sottocasa, E. Panfili, G. Sandri, G.F. Liut

On the nature of the compound responsible for mitochondrial calciummovements pag. 267

CIII M. Crompton

The activation of the sodium-calcium carrier of cardiac mitochondriaby potassium » 269

C III R. Kramer, M. Klingenberg

On the mechanism ofADP, ATP - translocation by the reconstitutedadenine nucleotide carrier » 271

C III G. Sachs, B. Wallmark, H.B. Stewart, M. Barcellona, T. Berglindh,G. Saccomani

Mechanistic aspects of the proton pump of gastric mucosa » 273

C HI A. Goffeau, J.P. Dufour, F. Foury, M. Boutry, A. Amory, A. Villa-loboIstheyeast plasma membrane ATPase a new type of proton pump?.... » 275

P III W.N. Konings, R. Otto, B. ten Brink, A.S.M. Sonnenberg, J. Hugen-holtz, H. VeldkampGeneration of an electrochemical proton gradient in Streptococcuscremoris and Escherichia coli by lactate efflux » 277

P HI C.V. Hammond, W.A. Hamilton, I.R. BoothMaintenance of intracellular pH in Escherichia coli » 279

P III R.G. Kroll, W.A. Hamilton, I.R. BoothMeasurement of proton movements linked to passive lactose and so-dium ion fluxes in Escherichia coli » 281

P HI E.P. BakkerThe role of A TP and the proton-motive force in potassium transportby the Escherichia coli TRKAsystem » 283

P III A. Siebers, L. Wieczorek, K. AltendorfPotassium transport in Escherichia coli: characterization of a pho-sphoryfated intermediate of the ATP- driven KDP system and topo-graphy of the subunits » 285

P III O. Misset, G.Th. RobillardStudies on the PEP- dependent phosphotransferase system of Escheri-chia coli: the mechanism of the enzyme-I catalyzed reaction » 287

P HI B.F. Luisi, H.J. Weber, J.K. LanyiSodium transport via sodium-proton antiport in Halobacterium halo-bium envelope vesicles » 289

P HI A. Fonyd, E. Ligeti, P.V. VignaisThe transport pathways for phosphate in mitochondria » 291

P III G.D. Mironova, N.I. Fedotcheva, P.R. Makarov, L.A. Pronevich,G.P. Mironov, M.N. KondrashovaThe protein from beef heart mitochondria inducing potassium per-meability of mitochondrial and artificial membrane pag. 293

P III R. KiehlOn the Co"'-phosphate symporter » 295

P III I. Roos, M. Crompton, E. CarafoliThe role of inorganic phosphate in the release of Ca1* from rat livermitochondria » 297

P III D. Siliprandi, M. Rugolo, A. Toninello, F. ZoccaratoOn the phosphate induced calcium transport in liver mitochondria » 299

P III H. WohlrabInhibitor-sensitivity of the reconstituted mitochondrial phosphatetransporter » 301

P HI K.S. Boos, E. SchlimmeADP, A TP transport in mitochondria. On the trigger function of theC(2' )hydroxyl group » 303

P HI Y. Briand, S. Touraille, S. Alziari, R. DurandDiscrimination between the phosphate carrier and the ATP/ADP car-rier in pig heart mitochondria : » 305

P III P. Dieterle, P. WalterAlanine transport in rat liver mitochondria » 307

P III J. Duszynski, U. Kiister, G. Letko, K. Bogucka, W. Kunz, L. Wojtc-zakIs the adenine nucleotide translocase in equilibrium ? » 309

P III E. Schlimme, K.S. BoosADP, ATP transport in mitochondria. On the role of the amidine re-gion for substrate binding and transport » 311

P III J.D. Arrabaca, R. Deana, J.B. ChappellAdenine nucleotides and the efflux of Ca2* from rat liver mitochondria » 313

P III P. Bernardi, G.F. AzzoneThe relationship between divalent cation transport and cytochrome cactivity in rat liver mitochondria » 315

P III F. Rigoni, R. Deana, J.D. Arrabaca, J,B. ChappellInhibitory effect of ruthenium red on calcium transport in rat liver mi-tochondria » 317

P III H.R. Lotscher, K.H. Winterhalter, E. Carafoli, C. RichterThe energy-state of mitochondria during transport of Ca1* » 319

P III J.W. Michejda, M. Hejnowicz, A. Domka-Popek, L. HryniewieckaChanges in membrane potential and Ca1* uptake in mitochondria ofAmoeba, snail heart and Ascaris muscles : pag. 321

P III G. Paradies, S. PapaThe effect ofCa2* on the translocation ofpyruvate in mitochondria.... » 323

P III N.E. Lofrumento, F. ZanottiNew aspects of the mechanism of anion substrate translocation in ratliver mitochondria » 325

P III I. Stipani, R. Kramer, F. Palmieri, M. KlingenbergReconstitution of citrate transport from rat liver mitochondria » 327

P III U.I. Flugge, H.W. HeldtOn the isolation of the phosphate translocator in chloroplasts » 329

P III M. Makinose, W. BollReaction sequence in the sarcoplasmic calcium transport (III) » 331

PHI B.J.W.M. Nieuwenhuis, C.A.G.M. Weijers, G.W.F.H. Borst-PauwelsThe plasma membrane ATPase is not directly involved in yeast diva-lent cation uptake » 333

P III A.S. Dahms, J.E. MiaraUP("O) - NMR kinetic analysis of the oxygen-18 exchange reactionbetween inorganic phosphate and water catalyzed by the (Na*, K*) -ATPase » 335

METABOLIC ASPECTS OF BIOENERGETICS

S IV H. TrachselThe mechanism of eukaryoticprotein synthesis initiation » 339

S IV A.S. SpirinEnergetics of the ribosome » 341

S VII R.J.A. Wanders, R.F. Nooteboom, A.J. Meijer, J.F. Nicholson,J.M. TagerIntramitochondrial A TPand its utilization for intra- and extra- mito-chondrial processes » 343

S VII H.E.S.J. Hensgens, A.J. Verhoeven, A.J. MeijerThe effect ofglucagon on the activity of carbamoyl-phosphate synthe-tase(ammonia) » 345

S VII H. SiesGlutathione: relationship to metabolism of reactive oxygen species » 347

S VII A.B. Wojtczak, K.S. Famulski, A. KielduckaThe mechanism ofglucagon action on mitochondrial functions pag. 349

C V F. Brawand, G. Folly, P. WalterRelation between extra- and intramitochondrial A TP/ADP ratios andoxygen uptake in rat liver mitochondria » 351

C V E. Marra, S. Passarella, S. Doonan, E. Quagliariello, C. SacconeThe selective Uptake of mitochondrial aspartate aminotransferase byisolated mitochondria: insensitivity to proteolytic digestion of the in-ternalized enzyme » 353

C V H.R. Lotscher, K.H. Winterhalter, E. Carafoli, C. RichterPeroxides, calcium, and pyridine nucleotides interactions in rat livermitochondria » 355

C V M.L. Eboli, T. GaleottiMitochondrial anion translocators in the shuttle systems of tumourcells » 357

C V L. TumermanEnergy conservation in nucleic acidsynthesis and turnover » 359

P VI R. LagunasEnergetic irrelevance ofaerobiosisforS. cerevisiae growing on sugars. » 361

P VI A.V. Kotelnikova, R.A. Zvjagilskaya, V.A. ZelenstchikovaRegulation of substrate oxidation in yeast mitochondria » 363

P VI L. Kovac, L. VareckaMembrane potential and metabolic control in yeast » 365

P VI E. Raddatz, P. KuceraSpatio-temporal patterns of the oxidative metabolism in the neurula-ting chick embryo » 367

P VI C.S. Lin, M. KlingenbergPurification and properties of the uncoupling protein from brown adi-pose tissue mitochondria » 369

P VI M.N. Kondrashova, E.I. Maevsky, I.B. Gusar, U.N. Anisimov, J.G.Kaminsky, E.A. KosenkoEndogenous succinate in mitochondria respiration under different sta-tes of organism » 371

P VI J. Popinigis, M. Kolanowska, M. Wozniak, T. WakabayashiUltrastructure of mitochondria under conditions ofcitrulline synthesis » 373

P VI B.D. Nelson, J. Kolarov, A. Wielburski, V. Joste, P. Gellerfors, 1.Mendel-HartvigBiosynthesis of mitochondrial proteins in isolated rat hepatocytes » 375

P VI M.J. Nalecz, A. Wroniszewska, K.S. Famulski, L. Wojtczak

On the mechanism of cuprjzone-induced formation of megamitochon-dria pag. 377

P VI K.A. Nalecz, A.B. Wojtczak

Transport of branched-chain a-ketoacids in isolated rat hepatocytesand liver mitochondria » 379

P VI Z. Machoy, G. Galka, T. Ogoriski, A. Stepinska

Comparison of properties of five ascorbic acid analogues used as redu-cingagents » 381

P VI J. Bryla, J.M. Dzik

Phosphoenolpyruvate efflux from rabbit renal cortex mitochondria.... » 3 83

P VI A. Kielducka, E. Walajtys-Rode

Metabolism of branched chain a-keto acids (BCKA) in hepatocytesfrom alloxan diabetic rats » 385

P VI A. Bindoli, L. Cavallini, P. Jocelyn

Formation and subsequent partial removal oflipid hydroperoxides inrat liver mitochondria » 387

P VI M. Markert, J. FreiOJOl stoichiometry of particle stimulated polymorphonuclear leuko-cytes » 389

P VI G.M. Bartoli, E. Bertoli, T. GaleottiElectron transport and superoxide radical production in tumor micro-somal membranes » 391

P VI J. Boonstra, C.L. Mummery, P.T. van der Saag, S.W. de Laat

Relationships between cation transport and growth in neuroblastomacells » 393

P VI Z. Kovacevic, M. BreberinaEnergy coupling of the mitochondria in intact ascites carcinoma cellsand the effect of oleate » 395

P VI K.S. Cheah, A.M. CheahEvidence for mitochondrial calcium - activated phospholipase in por-cine malignant hyperthermia » 397

P VI Z. Kaniuga, W.P. MichalskiMechanism of cold and dark - inactivation of chloroplast superoxided'ismutase activity and its effect on the photoperoxidation of membra-ne lipids » 399

P VI R. HamppPool sizes of adenine nucleotides during greening in mitochondria,

plastids, and a cytoplasmic fraction derived from plant cell protoplastwithin 60s, pag. 401

P VI M.C. Pugliarello, F. Rasi-Caldogno, M.I. De MichelisA TP-dependent hyperpolarization ofmicrosomal vesicles from pea in-ternodes » 403

P VI J.P. FlattComposition of expended energy and the regulation of energy balance » 405

P VI G. Curatola, L. Mazzanti, I. Pasquali-Ronchetti, E. Bertoli, G. LenazGeneral anesthetics as a tool for the study of lipid protein interactions. » 407

P VI Z. MasinovskyModel studies of some aspects ofprebiotic and early biological energe-tics » 409

P VI S. Costa, E. Costanzo, A. Rubbino, I. TurcoEntropy role in bio-systems » 411

PHOTOPIGMENT COMPLEXES

S VI P. MathisTransduction of the energy of light into chemical energy: the primaryprocesses •.• » 415

S VI W. Junge, H. SchaffernichtPhotochemical reaction centers from green plants » 417

S VI G. Feher, M. Schonfeld, M. MontalCharacterization of reaction centers (RCs)from Rhodopseudomonassphaeroides R-26 and their functional reconstitution into planar lipidbilayers » 419

S VI D. Oesterhelt, U. FischerChromophore structure and light inducedprotonation changes in bac-teriorhodopsin » 421

C IV D.B. Kell, A.M. GriffithsThe «energised state» in bacteriorhodopsin sheets » 423

C IV L. PackerEnergy conversion by bacteriorhodopsin » 425

C IV J.K. LanyiThe light-driven sodium pump of Halobactenumhaiobmm » 427

P V H.J. Weber, R.A. BogomolniPhotochemical cycle ofP,,,, a retinyl-pigment associated with sodiumtranslocation in a bacteriorhodopsin-deficient mutant of Halobacte-riumhalobium » 429

P V A. Fahr, E. Bamberg

Studies on photocurrent kinetics of purple membrane attached onblack lipid membranes pag. 431

P V V.P. Skulachev, L.A. Drachev, G.R. Kalamkarov, A.D. Kaulen,M.A. Ostrovsky

Animal rhodopsin as a photoelectric generator producing membranepotential to open a pore in photoreceptor membrane » 433

P V G. Wagner

Retinal-protein interactions and photosensory transduction in Halo-bacterium halobium » 435

P V D. Kuschmitz, M. Engelhard, R.B. Jones, B. Hess

Interactions between amino acid residues and the retinal chromophoreof bacteriorhodopsin » 437

P V G. Jolchine, F. Reiss-Husson

Interactions between isolated reaction centers and membranes from areaction center-less mutant o/Rhodopseudomonas sphaeroides » 439

P V N.G. Holmes, C.N. Hunter, R.A. Niederman, A.R. CroftsThe carotenoid shift of photosynthetic bacteria: its relationship withlight harvesting complexes » 441

P V T. Herczeg, I. Vass, L. SzalayDetermination of activation energies of thermoluminescence compo-nentsinChlorella » 443

P V J.H. Argyroudi-AkoyunoglouThe effect of monovalent and divalent cations on the chlorophyll di-stribution among the pigment-protein complexes of the thylakoid » 445

P V R.C. Jennings, F.M. Garlaschi, P.D. Gerola, G. FortiEffects of cations on thylakoid membranes » 447

BIOENERGETICS OF SPECIAL BACTERIAL SYSTEMS

S V C. Veeger, C. Laane, H. HaakerEnergy supply for dinitrogen fixation by A. Vinelandii and bacteroidso/R. leguminosarum » 453

S V R. Thauer, W. BadziongA TP synthesis in Desulfovibrio vulgaris (Marburg): stoichiometry andmechanism » 455

P IV G. Tikhonova, V. Artzatbanov, E. Mileikovskaja, T. Sheiko, D.OstrovskyStudies on spectral and potentiometrical properties of cytochromes b

and arrangement of factor BF, in Micrococcus lysodeikticus membra-nes pag.457

P IV M.J. Dawson, C.W. JonesEnergy conservation in Methylophilus methylotrophus » 459

P IV M.F. HenryElectron transfer associated with denitrification in Paracoccus denitri-ficans » 461

P IV M.F. Henry, F. Porte, P.M. VignaisEnergetics of electron transport from Hx in Paracoccus denitrificans... » 463

P IV J.A. Ward, O.T.G. JonesChanges in the electron transport system of Rhodopseudomonassphaeroides induced by dark, anaerobic growth » 465

P IV D. Zannoni, B.L. MarrsThe role of the redox chain during anaerobic-dark growth o/Rhodop-seudomonas capsulata » 467

P IV J.P. Armitage, M.C.W. EvansMeasurements of membrane potential changes during tactic stimula-tion of photosynthetic and non-photosynthetic bacteria using the ca-rotenoid bandshift and oxanol dyes » 469

APPENDIX

H. GestProposal for simplified nomenclature of electron transport and energyconversion processes in microbial and other systems » 473