scientist catalogue 2011 final - ku

Biomedicine, KU-LIFE

Scientist Catalogue 2011

At the Novo Nordisk-Life In Vivo Pharmacology Centre, 24 PhD and 5 post doc projects will be initiated in the period 2010-2015.

The purpose of this catalogue is to promote project collaboration between KU-LIFE, Section of Biomedicine and Novo Nordisk, on projects within In Vivo Pharmacology & Toxicology.

The catalogue consists of senior and junior faculty scientists at Biomedicine, KU-LIFE. Feel free to take the first contact.

Please note that PhD projects initiated within the LIFEPHARM Centre need a senior faculty member from Biomedicine as main supervisor.

To apply for a PhD fellowship from LIFEPHARM, you should submit a ‘Short Project Proposal’ to our Centre administrator—preferably in collaboration between Novo Nordisk and LIFE—using the form available on our website, www.lifepharm.dk . Here you can also find the deadline for submission. If the project is selected, you will be invited to submit a full PhD application (full application form is also available on the website).

We look forward to the collaboration

Jens Lykkesfeldt

Director of LIFEPHARM

Content: Part 1: Senior faculty, Section of Biomedicine, KU-LIFE Part 2: Junior faculty, Section of Biomedicine, KU-LIFE Contact information

Part 1: Senior Faculty at Biomedicine (i.e. possible main supervisors)

Alf Skovgaard Position: Associate Professor

Previous positions and background Employments: 2008 ‐ Associate professor, Aquatic pathobiology 2005‐2008 Associate professor, Section of Aquatic of Biology, Department of Biology, University of Copenhagen 2003‐2005 Post doc., Institut de Ciències del Mar, CSIC, Barcelona, Spain (EU ‐ Marie Curie fellowship) 2000‐2002 Post doc., Marine Biological Laboratory, University of Copenhagen Education: 1999 PhD in zoology (marine plankton ecology), Department of Biology, Marine Biological Laboratory, University of Copenhagen.

1995 Master of Science degree in biology, University of Copenhagen.

Brief description of research group and collaborators My main interest is within aquatic pathobiology and parasitology. I investigate the effect and ecology of aquatic pathogenic microorganisms, such as protozoans and small invertebrates. This is done both as laboratory experiments and through investigations of disease‐causing organisms in field samples. I work not only of fish disease, but also on diseases in fish eggs and fish larvae as well as aquatic invertebrates and zooplankton organisms. I also explore the molecular phylogeny and taxonomy of marine plankton organisms With focus on disease‐causing organisms.

Scientific interests ‐ Aquatic pathobiology ‐ Diseases of crustaceans ‐ Diseases and ecology of marine plankton organisms ‐ Aquatic protozoans and algae

Technological/Analytical platform and key competencies ‐ Experimental ecology of marine microorganisms ‐ Molecular methods in protistology ‐ Methods in molecular phylogeny ‐ Morphology through various microscopical techniques ‐ Histology and histochemistry ‐ Scanning electron microscopy

Selected papers Eigemann, F., Burmeister, A., Skovgaard, A. (2010) Detection of Hematodinium sp. (Alveolata, Syndinea) in crustaceans

from Danish and Greenlandic waters. Diseases of Aquatic Organisms. 92:59‐68.

Skovgaard, A., Meneses, I. & Angélico, M.M. (2009) Identifying the lethal fish egg parasite Ichthyodinium chabelardi as a

member of Marine Alveolate Group I. Environmental Microbiology. 11: 2030–2041.

Skovgaard, A. & Daugbjerg, N. (2008) Identity and systematic position of Paradinium poucheti and other Paradinium‐like

parasites of marine copepods based on morphology and nuclear encoded SSU rDNA. Protist. 159: 401‐413.

Skovgaard, A. & Saiz, E. (2006) Seasonal occurrence and role of protistan parasites in coastal marine zooplankton. Marine

Ecology Progress Series 327: 37‐49.

Skovgaard, A., Massana, R., Balagué, V. & Saiz, E. (2005) Phylogenetic Position of the Copepod‐Infesting Parasite

Syndinium turbo (Dinoflagellata, Syndinea). Protist. 156: 413‐423.

Skovgaard, A. (2005) Infection with the dinoflagellate Blastodinium in two Mediterreanean copepods. Aquatic Microbial

Ecology. 38: 93‐101.

Axel Kornerup Hansen Position Professor in laboratory animal science and welfare Head of section ‘Biomedicine’, chair ‘Centre for Applied Laboratory Animal Research’.

Previous positions and background Veterinarian, Roskilde Dyreklinik 1985 ‐ 1987 Head of Laboratory and Production, Møllegaard Breeding Center (Taconic M&B Ltd) 1987 ‐ 1992 Associate Director, Department of Experimental Medicine, KU‐SUND 1993 ‐ 1997 Professor, Department of Veterinary Disease Biology, KU‐LIFE 1997 ‐ ? Head of Section of Biomedicine, Department of Veterinary Disease Biology, KU‐LIFE, 2007 ‐ ?

Brief description of research group and collaborators My group consists of 1 associate professor, 1 post.doc., 2 animal technicians, and 5 PhD and 6 master students. Additionally I am co‐supervising 4 PhD and 2 master students. Main collaborators are Department of Food Science (bacteriology), other groups in Biomedicine (immunological/chemical methods), and groups on LeoPharma, Lundbeck and Novo Nordisk (animal models).

Scientific interests My main scientific interest is how early life microbiology influence animal models of inflammatory diseases, e.g. diabetes, atherosclerosis, colitis, dermatitis and neuroinflammaton. The vision is to fully control the early life gut microbiota and thereby reduce the variation or increase the power of experimental animal studies. I work mostly in mice and pigs, and to a minor extent in rats. Keywords: Gut microbiota, animal models, inflammatory diseases, 3R’s, experimental design.

Technological/Analytical platform and key competencies Modelling human diseases in animals, gut microbiota and inflammatory response characterization.

Selected papers 1.Dahl K, Kirkeby S, d'Apice AJF, Matthiassen S, Hansen AK. The Bacterial Flora of alpha‐Gal Knock Out Mice Express the alpha‐Gal Epitope Comparable to Wild Type Mice. Transpl Immunol 2005;14(1):9‐16. 2.Hansen AK, Ling F, Kaas A, Funda DP, Farlov H, Buschard K. Diabetes preventive gluten‐free diet decreases the number of caecal bacteria in non‐obese diabetic mice. Diabetes‐Metabolism Research & Reviews 2006;22(2):220‐5. 3.Kirkeby S, Hansen AK, d'Apice A, Moe D. The galactophilic lectin from Pseudomonas aeruginosa. Its binding requirements and the localization of lectin receptors in various mouse tissues. Microb Pathogene 2006;40(5):191‐7. 4.Sangild PT, Siggers RH, Schmidt M, Elnif J, Bjornvad CR, Thymann T, et al. Diet‐ and colonization‐dependent intestinal dysfunction predisposes to necrotizing enterocolitis in preterm pigs. Gastroenterology 2006;130(6):1776‐92. 5.Dahl K, Buschard K, Gram DX, d'Apice AJF, Hansen AK. Glucose intolerance in a xenotransplantation model: studies in alpha‐gal knockout mice. APMIS 2006;114(11):805‐11. 6.Kirkeby S, Wimmerova M, Moe D, Hansen AK. The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA‐IL and PA‐IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink. Microbes Infect 2007 Apr;9(5):566‐73. 7.Siggers RH, Thymann T, Siggers JL, Schmidt M, Hansen AK, Sangild PT. Bacterial colonization affects early organ and gastrointestinal growth in the neonate. Livestock Science 2007;109(1‐3):14‐8. 8.Olsson IAS, Hansen AK, Sandoe P. Ethics and refinement in animal research. Science 2007;317(5845):1680. 9.Ejsing‐Duun M, Josephsen J, AASTED B, Buschard K, Hansen AK. Dietary Gluten Reduces the Number of Intestinal Regulatory T Cells in Mice. Scandinavian Journal of Immunology 2008;67(6):553‐9. 10.Kay H, Murrell KD, Hansen AK, Madsen H, Nguyen TTT, Nguyen MH, et al. Optimization of An Experimental Model for the Recovery of Adult Haplorchis Pumilio (Heterophyidae: Digenea). Journal of Parasitology 2009;95(3):629‐33. 11.Varga O, Harangi M, Olsson IAS, Hansen AK. Contribution of animal models to the understanding of the metabolic syndrome: a systematic overview. Obesity Reviews 2010;11(11):792‐807. 12.Engkilde K, Buschard K, Hansen AK, Menne T, Johansen JD. Prevention of Diabetes in NOD Mice by Repeated Exposures to a Contact Allergen Inducing a Sub‐Clinical Dermatitis. Plos One 2010;5(5). 13.Hufeldt MR, Nielsen DS, Vogensen FK, Midtvedt T, Hansen AK. Variation in the Gut Microbiota of Laboratory Mice Is Related to Both Genetic and Environmental Factors. Comparative Medicine 2010;60(5):336‐42. stematic inter‐individual variation in the gut microbiota of inbred laboratory mice. Labor Animals 2010;44(4):283‐9.


Bent Aasted Position Professor in Veterinary Immunology., Ph.D, Dr. Sci., Dr. Vet. Sci.

Previous positions and background 74/08‐76/02 Post doc., The Rockefeller Univ. New York, USA 82/09‐83/09 Visiting associate. Lab. of Persistent Viral Diseases, NIAID, NIH, USA 86/07‐91/06 Research Professor, Dept. Virol. Immunol. (RVAU) 97/07‐98/06 Guest Professor, CSIRO, Dept. Animal Health, Parkville, Melbourne, Australia. Since 94/04 Professor, Immunological lab., Dept. Vet. Pathobiol. (KU)

Brief description of research group and collaborators My group consists of 6 staff members. I am main supervisor of 2 PhD students and co‐supervisor of 2. I have pt 2 (biotech) master students. My various collaborations fall mostly into two categories; Veterinary Immunology and Viral Pathogenesis.

Scientific interests Appx 50 articles concerns parvovirus infections (Aleutian Disease Virus) and appx 30 in swine immunology. My recent interest is in experimental influenza virus infection of ferrets, where the efficiency of new adjuvant formulations are being analysed.

Technological/Analytical platform and key competencies Characterization of monoclonal antibodies towards CD structures on cells from Veterinary relevant animals. Flowcytometic analyses of cellular receptor proteins and cytokine reasurements

Selected papers 111 McKenna, R., Olson, N.H., Chipman, P.R., Baker, T.S., Booth, T.F., Christensen, J., Aasted, B., et al. (1999) Three‐dimensional structure of Aleutian mink disease parvovirus: Implications for disease pathogenicity. J. Virol. 73, 6882‐6891. 118. Jensen, P. V., Castelruiz, Y. and Aasted, B. 2003. Cytokine profiles in adult mink infected with Aleutian Mink Disease Parvovirus. J. Virol. 77, 7444‐7451 121. Castelruiz, Y., Blixenkrone‐Møller, M. & Aasted, B. 2005. DNA vaccination with the Aleutian Mink Disease virus NS1 gene confers partial protection against disease. Vaccine 23, 1225‐1231. 122. Zola, Heddy, Bernadette Swart, Ian Nicholson, Bent Aasted et al. 2005. CD Molecules 2005: Human cell differentiation molecules. Blood 106, 3123‐6. 133. Saalmuller A, & Aasted, B.: 2007. Summary of the animal homologue of HLDA8. Vet. Immunol. Immunopathol. 119, 2‐13. 141. Jensen, TH, Nielsen, L., Aasted, B., Blixenkrone‐Møller. 2009. Early life DNA vaccination with the H gene of Canine distemper virus induces rebust protection against destemper. Vaccine 27: 5178‐5183. 146 Bragstad, Karoline ; Cyril Jean‐Marie Martel ; Bent Aasted ; Lars Peter Nielsen ; Anders Fomsgaard (2011) 1918 pandemic H1N1 DNA vaccine protects ferrets against 2007 H1N1 virus infection. Influenza and other respiratory viruses 5, 13‐23. 148 Cyril Martel; Else Marie Agger; Julie Juul Poulsen; Trine Hammer Jensen; Lars Andresen; Dennis Christensen; Lars Peter Nielsen; Merete Blixenkrone‐Møller; Peter Andersen; Bent Aasted. 2011. CAF01 Potentiates Immune Responses and Efficacy of an Inactivated Influenza Vaccine in Ferrets. Submitted to PLoS ONE February 2011


Christian Friis

Position

Professor in Pharmacology and Toxicology, Department of Veterinary Disease Biology, Faculty of Life Science, University of Copenhagen

Previous positions and background Veterinarian 1975, PhD in pharmacology 1977

Brief description of research group and collaborators The group comprises 2 PhD students, 4 Master students, 2 Technicians and 1 Professor.

Scientific interests

Transport and metabolism of xenobiotics

Comparative aspects of the cytochrome P450 system

Clinical pharmacology of antimicrobials and anthelmentics in food producing animals

PK/PD modelling

Technological/Analytical platform and key competencies In vivo animal experiments ( rodents, domestic animals), cell culture of hepatocytes and proximal tubules of the nephron, radio‐labelled test‐substances, HPLC

Selected papers 1: Winther L, Baptiste KE, Friis C. Pharmacokinetics in pulmonary epithelial lining fluid and plasma of ampicillin and pivampicillin administered to horses. Res Vet Sci. 2010 Dec 6. [Epub ahead of print] PubMed PMID: 21144541. 2: Skaanild MT, Friis C. Is bupropion a more specific substrate for porcine CYP2E than chlorzoxazone and p‐nitrophenol? Basic Clin Pharmacol Toxicol. 2007 Sep;101(3):159‐62. PubMed PMID: 17697034. 3: Loven AD, Olsen AK, Friis C, Andersen B. Phase I and II metabolism and carbohydrate metabolism in cultured cryopreserved porcine hepatocytes. Chem Biol Interact. 2005 Jun 30;155(1‐2):21‐30. PubMed PMID: 15950209. 4: Lauritzen B, Lykkesfeldt J, Skaanild MT, Angen Ø, Nielsen JP, Friis C. Putative biomarkers for evaluating antibiotic treatment: an experimental model of porcine Actinobacillus pleuropneumoniae infection. Res Vet Sci. 2003 Jun;74(3):261‐70. PubMed PMID: 12726745. 5: Skaanild MT, Friis C. Is cytochrome P450 CYP2D activity present in pig liver? Pharmacol Toxicol. 2002 Oct;91(4):198‐203. PubMed PMID: 12530471. 6: Lindecrona RH, Friis C, Nielsen JP. Pharmacokinetics and penetration of danofloxacin into the gastrointestinal tract in healthy and in Salmonella typhimurium infected pigs. Res Vet Sci. 2000 Jun;68(3):211‐6. PubMed PMID: 10877965. 7: Agersø H, Friis C, Nielsen JP. Penetration of amoxycillin to the respiratory tract tissues and secretions in Actinobacillus pleuropneumoniae infected pigs. Res Vet Sci. 1998 May‐Jun;64(3):251‐7. PubMed PMID: 9690612. 8: Friis C. Penetration of danofloxacin into the respiratory tract tissues and secretions in calves. Am J Vet Res. 1993 Jul;54(7):1122‐7. PubMed PMID: 8396371. 9: Friis C. Postnatal development of renal function in piglets: glomerular filtration rate, clearance of PAH and PAH extraction. Biol Neonate. 1979;35(3‐4):180‐7. PubMed PMID: 435594.


Dan Holmberg Position Professor MSO biotechnology KU‐LIFE 2008 (80%). Professor in Medical Genetics, Umeå University, since 1999 (20%).

Previous positions and background Professor in medical genetics, Umeå University 1999 ‐ present Assoc professor in immunology, Umeå University, 1986‐1999. EMBO long term fellow, Institut Pasteur, Paris, 1984‐1986. Bachelor in medicine (Med kand.) 1980, PhD in Immunology 1984, Docent in Immunology 1985.

Brief description of research group and collaborators My group consists of 4 post docs ( 1 in Umeå), 1 technician (in Umeå) two research assistants and a PhD student. I am also co‐supervisor of 1 post doc and 2 PhD students. We have multiple collaborations within KU‐LIFE (in the area of immunology of parasite infections and autoimmunity and in imaging of inflammatory processes) KU‐Sund (mainly related to diabetes) and KU‐Pharma (mainly EAE). We also have a broad network of international collaborations mainly in the area of imaging and autoimmune inflammation including partners at Umeå University, KI, Stockholm, Lund University, Cambridge University, Hospital for Sick Kids, Toronto, and others.

Scientific interests My main scientific interest is to understand the cellular and molecular mechanisms underlying autoimmune inflammation. We use a multifaceted approach including real time live imaging, and 3‐D, quantitative imaging in combination with cellular immunology and molecular genetics to study animal models of autoimmune inflammation (particular focus is on the NOD mouse and the mouse EAE models). We also work in parallel with functional studies of immunephenotypes in human families with type 1 diabetes and autoimmune thyroid disease (mainly in Umeå). Keywords: Type 1 diabetes; EAE; autoimmune inflammation; plasmacytoid dendritic cells; autoinflammation.

Technological/Analytical platform and key competencies Animal models of autoimmune inflammation, immunophenotyping, 3‐D imaging, live imaging.

Selected papers 76. Cilio, C.M., M.R. Daws, A. Maleshnikova, C.L. Sentman and D. Holmberg CTLA‐4 is induced in the thymus upon in vivo activation and its blockade prevents anti‐CD3 mediated depletion of thymocytes. J. Exp. Med. 188:1239‐1246, 1998 94. Bagot, S., Campino, S., Penha‐Goncalves, C., Pied, S., Cazenave, P‐A. and Holmberg, D. Identification of two novel cerebral malaria resistance loci using an inbred wild derived mouse strain Proc Natl Acad.Sci USA 99:9919‐9923, 2002 98. Bergman, M‐L., Duarte, N.,Campino, S.,Lundholm, M., Lejon, K., Penha Gonçalves, C. and Holmberg, D. Diabetes protection and restoration of apoptosis resistance in NOD Idd6 congenic strains Diabetes 52: 1677‐1682, 2003 103. Rolf, J., Motta, V., Duarte, N., Lundholm, M., Berntman, E., Bergman, M‐L., Sorokin, L., Cardell, S. and Holmberg, D. The enlarged population of marginal zone/CD1dhigh B lymphocytes in non‐obese diabetic mice appears at the time of disease initiation and maps to diabetes susceptibility region Idd11 J. Immunol. 174:4821‐4827, 2005 111. Lundholm, M., Motta, V., Löfgren‐Burström, A., Duarte, N., Bergman, M‐L., Eklund, S. and Holmberg, D. Defective induction of CTLA‐4 in the NOD mouse is controlled by the NOD allele of Idd3/IL‐2 and a novel locus (Ctex) telomeric on chromosome 1. Diabetes. 55:538‐44, 2006 112. Einarsdottir, E., Mayans, S., Ruikka, K., Escher S. A. , Lindgren, P., Ågren, Å., Eliasson M. and Holmberg, D. Linkage but not Association of Calpain‐10 to Type 2 Diabetes Replicated in Northern Sweden. Diabetes 55:1879‐1883. 2006 116. Wikstrom I, Forssell J, Goncalves M, Colucci F, Holmberg D. E2‐2 regulates the expansion of pro‐B cells and follicular versus marginal zone decisions. J Immunol. 177:6723‐9. 2006 118. Alanentalo T, Asayesh A, Morrison H, Loren CE, Holmberg D, Sharpe J, Ahlgren, U. Tomographic molecular imaging and 3D quantification within adult mouse organs. Nat Methods. 4:31‐3. 2007 129. Cisse, B., Caton, M.L., Lehrer, M., Maeda, T., Scheu, S., Locksley, R., Holmberg, D., Zweier, C., den Hollander, N.S., Kant, S.G., Holter, W., Rauch, A., Zhuang, Y., Reizis, B. Transcription factor E2‐2 is an essential and specific regulator of plasmacytoid dendritic cell development. Cell 135:37‐48, 2008 134. Lundholm, M., Mayans, S., Motta, V., Löfgren‐Burström, A., Danska, J. and Holmberg, D. Variation in the Cd3ζ (Cd247) gene affects T cell activation and is associated with autoimmune diabetes. J. Immunol. 184: :5537‐4444, 2010 135. Alanentalo, T., Hörnblad, A., Mayans, S., Nilsson, A. K., Sharpe, J., Larefalk, Å., Ahlgren., U. and Holmberg D. Quantification and 3‐D imaging of the insulitis‐induced destruction of beta‐cells in murine Type 1 Diabetes. Diabetes 59:1756‐64.2010

Dorte Bratbo Sørensen Position Associate professor in Laboratory Animal Science and Welfare.

Previous positions and background Assistant professor, KU‐LIFE 2001 Teaching assistant 1993, 1994, 2000 Veterinarian at a Small animal hospital, slaughter house and shelter for homeless animals 1993‐1995 DVM in 1993, PhD in ethology and animal welfare 2001

Brief description of research group and collaborators I am part of Axel Kornerup Hansens group. I am main supervisor of 2 PhD students and co‐supervisor of 1. My collaborations fall mostly into two categories; Animal welfare/Refinement (EU‐project; Centre of Applied Lab Animal Research; Center of BioEthics & Risk assessment) and Improvement of behavioural tests in neuroscience (LabResearch, several external/internal project partners)

Scientific interests My main scientific interests are Animal welfare – both in theory, as an area of research and as a practical application in the lab animal facility ‐ and Refinement of behavioural models through biological optimization and use of positive motivation. My latest area‐of‐interest is the impact of gut microbiota on neuroinflammation and behaviour. Keywords: Animal welfare, Refinement, behavioural models in neuroscience, gut microbiota & behaviour, positive reinforcement training (PRT, “clicker‐training”),

Technological/Analytical platform and key competencies Assessment of animal welfare through preference testing and evaluation of biological functioning, PRT of pigs and dogs, behavioural characterization/testing of animal models.

Selected papers D B Sørensen (2010) Never wrestle with a pig ... Lab Anim, 44, 159‐161.

LF Mikkelsen, DB Sørensen, TC Krohn, B Lauritzen, N Dragsted, AK Hansen, JL Ottesen (2010) Clinical pathology and cardiovascular parameters are not influenced by housing rats under increased environmental complexity. Anim Welf, 19, 449‐460.

DB Sørensen et al. (2010) The influence of three standardized enriched environments on learning and memory abilities in group‐housed SD. Scand J Lab Anim Sci, in press

D Sørensen et al. (2010) Preferences for limited versus no contact in SD rats Lab Anim, 44, 274‐277

Kasanen IHE, Sørensen DB, Forkman B, Sandøe P (2010) Ethics of feeding: the omnivore dilemma Anim Welf, 8, 37‐44

Huber R, Sørensen DB, Sandøe P, Whitelaw B, Lillico S, Carlisle A, Olsson AIS (2010) Welfare protocols for large transgenic animals Transgenic res 19, p 138

Sørensen DB, Møller M, Larsen L (2009) The use of the Tecniplast mousehouse in four strains of mice Scand J Lab Anim Sci 36, 2, 179‐183

Sørensen DB, Mortensen K, Bertelsen T, Vognbjerg K (2008) Enriching the metabolic cage: effects on physiology and behaviour. Anim Welf, 17, 395‐403

Sørensen DB, Dahl K, Ersbøll AK, Kirkeby S, D’Apice A, Hansen AK (2008) Aggression in cataract‐bearing alfa‐1,3‐galactosyltransferase knockout mice. Lab Anim 42, 34‐44

Sørensen DB, Stub C; Jensen HE, Ritskes‐Hoitinga M, Thon R, Hjorth P, Ottesen JL Hansen AK (2007) The impact of tail tip amputation and ink tattoo on C57Bl/6JBomTac mice. Lab Anim, 41, 19‐29.

Krohn T, Sørensen DB, Ottesen JL, Hansen AK. (2006) Single housing of mice and rats ‐ A review. Anim Welf, 15, 343‐352.

Sørensen DB, Stub C, Jegstrup IM, Ritskes‐Hoitinga M, Hansen AK (2005) Fluctuating asymmetry in relation to stress and social status in inbred male Lewis rats. Scand J Lab Anim Sci 32, 117‐23.

Sørensen DB, Krohn T, Ottesen JL, Hansen AK. (2005) An ethological approach to housing requirements of hamsters and gerbils in the laboratory ‐ A review. Appl Anim Behav Sci 94, 181‐95.

Sørensen DB, Johnsen, PF, Bibby MB, Böttner A, Bornstein S, Hansen AK (2005) Phenylethanolamin‐N‐methyl‐transferase (PNMT) transgenic mice have an aggressive phenotype. Horm Met Res, 37, 159‐163.

Jens Lykkesfeldt Position Professor in experimental pharmacology & toxicology. Head of the in vivo pharmacology PhD program, director of LIFEPHARM.

Previous positions and background Professor MSO in molecular toxicology, KU‐LIFE, 2008 Assoc professor in pharmacology, KU‐LIFE, 1998‐2007. Visiting research associate, University of California, Berkeley, USA, 1996‐98. Research assistant professor in pharmacology, KU‐SUND, 1993‐95. MSc in chemistry 1989, PhD in Biochemistry 1992, DSc in medicine 2005.

Brief description of research group and collaborators My group consists of about 15 staff members with a broad range of scientific backgrounds. I am main supervisor of 7 PhD students and co‐supervisor of 4. My various collaborations fall mostly into two categories; in vivo studies using animal models of disease and clinical studies in humans.

Scientific interests My main scientific interest is oxidative stress in disease. Rather than to focus on any particular aspect alone, I prefer larger setups that attempt to encompass molecular, biochemical, in vivo and clinical aspects of a particular problem. However, this may not necessarily all be in one project. Keywords: Metabolic syndrome; early brain development; oxidative stress and inflammation; vitamin C deficiency; endothelial dysfunction; pharmacokinetics.

Technological/Analytical platform and key competencies Development and quantification of biomarkers of oxidative stress and damage; HPLC of labile compounds; rodent models of disease, pharmacokinetics.

Selected papers ‐ Frikke‐Schmidt et al. (2011) High dietary fat and cholesterol exacerbates chronic vitamin C deficiency in guinea pigs.

Brit. J. Nutr., 105, 54‐61. ‐ Tveden‐Nyborg et al. (2009) Vitamin C deficiency induces impaired neuronal and cognitive development in neonatal

guinea pigs. Am. J. Clin. Nutr. 90, 540‐546. ‐ Odermarsky et al. (2009) Poor vit C status is associated with increased carotid intima‐media thickness, decreased

microvascular function and delayed myocardial repolarization in young patients with type 1 diabetes. Am. J. Clin. Nutr. 90, 447‐452.

‐ Lykkesfeldt et al. (2007) Oxidative stress and damage in liver, but not in brain, of fischer 344 rats subjected to dietary iron supplementation with lipid‐soluble [(3,5,5‐trimethylhexanoyl)ferrocene]. J. Biochem.Mol. Toxicol. 21, 145‐155.

‐ Lykkesfeldt et al. (2007) Vitamin C deficiency in weanling guinea pigs: Differential expression of oxidative stress and DNA repair in liver and brain. Brit. J. Nutr., 98, 1116‐1119.

‐ Christen et al. (2005) Oxidative stress precedes systemic inflammatory response in pediatrics undergoing cardiopulmonary bypass operation. Free Radic. Biol. Med. 38, 1323‐1332.

‐ Jiang et al. (2002) ‐Tocopherol supplementation inhibits protein nitration and ascorbate oxidation in rats with inflammation. Free Radic. Biol. Med. 33, 1534‐1542.

‐ Lykkesfeldt (2002) Increased oxidative damage in vitamin C deficiency is accompanied by induction of ascorbic acid recycling capacity in young but not mature guinea pigs. Free Radic. Res. 36, 567‐574.

‐ Schaper et al. (2002) Cerebral vasculature is the major target of oxidative protein alterations in bacterial meningitis. J. Neuropath. Exp. Neur. 61, 605‐613

‐ Hagen et al. (2002) Feeding acetyl‐L‐carnitine and lipoic acid to old rats significantly improves metabolic function while decreasing oxidative stress. Proc. Natl. Acad. Sci. USA. 99, 1870‐1875.

‐ Christen et al. (2001) Brain oxidative stress in experimental bacterial meningitis: Differential effects of ‐phenyl‐tert‐butyl nitrone and N‐acetylcysteine treatment. Free Radic. Biol. Med. 31, 754‐762.

‐ Hagen et al. (1999) (R)‐‐lipoic acid supplemented old rats have improved mitochondrial function, decreased oxidative damage, and increases metabolic rate. FASEB J. 13, 411‐418.

‐ Lykkesfeldt et al. (1998) Age‐associated decline in ascorbic acid concentration, recycling and biosynthesis in rat

hepatocytes—Reversal with (R)‐‐lipoic acid supplementation. FASEB J. 12, 1183‐1189.


Kurt Buchmann Position Professor in Aquatic Pathobiology since 2005 Head of the Danish Fish Immunology Research Centre and Network DAFINET (www.dafinet.dk) Head of the Research School SCOFDA

Previous positions and background Professor MSO in Fish Immuno‐Parasitology, KU‐LIFE, 2000‐2005, Associate professor in Fish Diseases, KU‐LIFE, 1995‐2000, Scientific assistant 1992‐1995 in Fish Parasitology, KU‐LIFE, Senior research fellow 1989‐1992 (Fish immuno‐parasitology) KU‐LIFE MSc in Aquatic Parasitology at KU‐NAT 1983, PhD in Fish Parasitology 1989, DVSc 1997.

Brief description of research group and collaborators My group consists of about 16 staff members with different scientific backgrounds but mostly biologists. I am principal supervisor of 6 PhD students. Collaborators are found among national and international research institutions and companies (vaccinology, nutrition, immunology).

Scientific interests My main scientific interest is the interaction between the fish immune system and pathogens in the light of the phylogenetic and ontogenetic development of hosts. Keywords: Fish as models for higher vertebrates, Ontogenetic development of the immune system, Phylogeny of the vertebrate immune system, Expression of immune related genes, Immune effector molecules.

Technological/Analytical platform and key competencies Expression of immune genes by qPCR, Immunohistochemistry, Immunocytochemistry

Selected papers from 2007‐2011 Gonzales et al. (2007) Real‐time gene expression analysis in carp (Cyprinus carpio L.) skin: Inflammatory responses caused

by the ectoparasite Ichthyophthirius multifiliis. Fish & Shellfish Immunol 22: 641‐650 Gonzales et al. (2007) Ichthyophthirius multifiliis infection induces massive up‐regulation of serum amyloid A in carp

(Cyprinus carpio). Vet. Immunol. Immunopathol. 115: 172‐178. Kania et al. (2007) Baltic salmon activates immune relevant genes in fin tissue when responding to Gyrodactylus salaris

infection. Dis. Aquat. Org. 76: 81‐85 Raida & Buchmann (2007) Temperature‐dependent expression of immune‐relevant genes in rainbow trout following

Yersinia ruckeri vaccination. Dis. Aquat. Org. 77:41‐52 Huyse et al. (2008). The mitochondrial genome of Gyrodactylus derjavinoides (Platyhelminthes: Monogenea) – a

mitogenomic approach for Gyrodactylus species and strain identification. Gene 417: 27‐34. Raida & Buchmann (2008). Development of adaptive immunity in trout (Oncorhynchus mykiss Walbaum) surviving and

infection with Yersinia ruckeri: Fish & Shellfish Immunology 25: 533‐541. Jørgensen et al. (2008). Immune‐relevant genes expressed in rainbow trout following immunisation with a live vaccine

against Ichthyophthirius multifiliis. Dis. Aquat. Org. 80: 189‐197. Raida & Buchmann (2009). Innate immune responses of rainbow trout (Oncorhynchus mykiss Walbaum) against primary

and secondary infections with Yersinia ruckeri O1. Developmental and Comparative Immunology 33:35‐45 Chettri et al. (2010). Factors influencing in vitro respiratory burst assays with head kidney leucocytes from rainbow trout,

Oncorhynchus mykiss (Walbaum). J. Fish Dis. 33: 593‐602. Kania et al. (2010). Divergence between Asian, European and Canadian populations of the monogenean

Pseudodactylogyrus bini indicated by ribosomal DNA patterns. J. Helminthol. 84: 404‐409. Kania et al. (2010) Molecular and immunohistochemical studies on epidermal responses in Atlantic salmon Salmo salar L.

Induced by Gyrodactylus salaris Malmberg, 1957. Journal of Helminthology 84, 166‐172. Olsen et al. (2011). Cellular and humoral factors involved in the response of rainbow trout gills to Ichthyophthirius

multifiliis infections: Molecular and immunohistochemical studies. Fish and Shellfish Immunology (in press). Chettri et al. (2011) PAMP induced expression of immune relevant genes. Developmental and Comparative Immunology

(In press) Woo & Buchmann Eds. (2011). Fish Parasites: Pathobiology and Protection. CABI, UK.


Lisbeth Høier Olsen Position Professor mso in clinical pharmacology and toxicology, KU LIFE, 2011 ‐

Previous positions and background Associate professor in veterinary physiology, KU‐LIFE, 2003‐2011. Assistant professor in veterinary physiology, KU‐LIFE, 2000‐2003. DVM 1996, DVSc 2005.

Brief description of research group and collaborators I am main supervisor of 3 PhD students and co‐supervisor of 2. I have close collaboration with assistant professor Sophia Moesgaard at IBHV (KU LIFE) regarding vascular biology. Our main collaborators are Aarhus University Hospital (Skejby), Copenhagen University Hospital (Rigshospitalet), The Swedish University of Agricultural Sciences and University of Pennsylvania.

Scientific interests My main scientific interest is in vivo cardiovascular animal models especially cardiovascular imaging, 24 hour electrocardiography (Holter monitoring), platelet function, cardiovascular biomarkers (in blood and tissue), histopathology and vascular function. My group has experience with spontaneous cardiovascular animal models (privately owned dogs) and experimental models (pigs). Keywords: Cardiovascular imaging, electrocardiography, platelet function, cardiovascular biomarkers and histopathology.

Technological/Analytical platform and key competencies Cardiovascular imaging (conventional echocardiography and advanced analyses of myocardial function (speckle tracking and tissue Doppler imaging); vascular imaging (flow‐mediated vasodilation (FMD)), 24 hour electrocardiography (arrhythmia and heart rate variability analysis), platelet analysis (optical and whole blood platelet aggregation; platelet function analyzer (PFA 100)), measurements of cardiovascular biomarkers and cardiovascular histopatphology

Selected papers

1. Olsen LH, Kristensen AT, Häggström J, Jensen AL, Klitgaard B, Hansson H, Pedersen HD. Increased platelet aggregation in non‐thrombocytopenic Cavalier King Charles Spaniels with mitral valve prolapse. Journal of Veterinary Internal Medicine 2001;15:209‐21. 2. Olsen LH, Martinussen T, Pedersen HD. Early echocardiographic predictors of myxomatous mitral valve disease in dachshunds. Veterinary Record 2003;152:293‐297. 3. Olsen LH, Mortensen K, Martinussen T, Larsson LI, Baandrup U, Pedersen HD. Increased NADPH‐diaphorase activity in canine myxomatous mitral valve leaflets. Journal of Comparative Pathology 2003;129:120‐130. 4. Moesgaard SG, Holte AV, Mogensen T, Mølbak J, Kristensen AT, Jensen AL, Teerlink T, Reynolds AJ, Olsen LH. Effects of breed, gender, exercise and white‐coat effect on markers of endothelial function in dogs. Research in Veterinary Science 2007;82:409‐15. 5. Pedersen LG, Zhao J, Yang J, Thomsen PD, Gregersen H, Hasenkam JM, Smerup M, Pedersen HD, Olsen LH. ”Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets”. Research in Veterinary Science 2007;82:232‐8.6. Falk T, Jönsson L, Olsen LH, Tarnow I, Pedersen HD. Association between cardiac pathology and clinical findings in dogs with naturally occurring chronic congestive heart failure. The Veterinary Journal 2010: Jul;185:68‐74.7. Rasmussen CE, Vesterholm S, Ludvigsen TP, Häggström J, Pedersen HD, Moesgaard SG, Olsen LH. Holter monitoring in clinically healthy cavalier King Charles spaniels, wire‐haired dachshunds and cairn terriers. Journal of Veterinary Internal Medicine 2011 (in press).


Martin Kristian Raida Position Associate Professor in Aquatic Pathobiology

Previous positions 2008 – 2009. Post doc. (FTP grant) at laboratory of Aquatic Pathobiology, Section of Biomedicine, Department of Veterinary Pathobiology, Faculty of Life Sciences, University of Copenhagen (KU‐LIFE). 2005‐ 2008 PhD‐student at laboratory of Aquatic Pathobiology, KU‐LIFE 2004 Research assistant at laboratory of Aquatic Pathobiology, Department of Veterinary Pathobiology, KVL.

Brief description of research group and collaborators Our group consists of 16 staff members, mainly working in the field of fish immunology (See DAFINET (Danish Fish Immunology Research Network) www.dafinet.dk). I have ongoing research projects investigating the immune response in rainbow trout after vaccination and infection with fish pathogenic bacteria. I am supervisor for two PhD students (Kasper Rømer Villumsen and Sidhartha Deshmukh ). They are both testing vaccines against fish pathogene bacteria in in vivo fish models. My collaboration is focused on mucosal immunology, in vivo infection models and vaccine development.

Scientific interests My main scientific interest is to understand development of immunity in vaccinated fish. My research is mainly focused on understanding the protective mechanisms behind success full immersion vaccination, using rainbow trout as in vivo model. Scientific focus areas: • Fish immunology • Vaccine development • In vivo infection models

Technological/Analytical platform and key competencies

Bacterial infection models (including GMO)

Bioimageing on whole fish (Optical Projection Tomography (OPT) technology)

Transcriptional analysis (RT‐qPCR using TaqMan probes)

Immunohistochemistry and development of antibodies

ELISA

Selected papers PAMP induced expression of immune relevant genes in head kidney leukocytes of rainbow trout (Oncorhynchus

mykiss). Chettri JK, Raida MK, Holten‐Andersen L, Kania PW, Buchmann K. Dev Comp Immunol. 2010 Dec 13.

Antigen‐sampling cells in the salmonid intestinal epithelium. Fuglem B, Jirillo E, Bjerkås I, Kiyono H, Nochi T, Yuki Y, Raida MK, Fischer U, Koppang EO. Dev Comp Immunol. 2010 Jul;34(7):768‐74. Epub 2010 Mar 1.

Response of rainbow trout (Oncorhynchus mykiss) in skin and fin tissue during infection with a variant of Gyrodactylus salaris (Monogenea: Gyrodactylidae). Jørgensen TR, Raida MK, Kania PW, Buchmann K. Folia Parasitol (Praha). 2009 Dec;56(4):251‐8

Immune‐relevant genes expressed in rainbow trout following immunisation with a live vaccine against Ichthyophthirius multifiliis. von Gersdorff Jorgensen L, Nemli E, Heinecke RD, Raida MK, Buchmann K. Dis Aquat Organ. 2008 Aug 7;80(3):189‐97.

Innate immune response in rainbow trout (Oncorhynchus mykiss) against primary and secondary infections with Yersinia ruckeri O1. Raida MK, Buchmann K. Dev Comp Immunol. 2009;33(1):35‐45.

Development of adaptive immunity in rainbow trout, Oncorhynchus mykiss (Walbaum) surviving an infection with Yersinia ruckeri. Raida MK, Buchmann K. Fish Shellfish Immunol. 2008 Nov;25(5):533‐41. Epub 2008 Jul 23.

Bath vaccination of rainbow trout (Oncorhynchus mykiss Walbaum) against Yersinia ruckeri: effects of temperature on protection and gene expression. Raida MK, Buchmann K. Vaccine. 2008 Feb 20;26(8):1050‐62. Epub 2008 Jan 10.

Temperature‐dependent expression of immune‐relevant genes in rainbow trout following Yersinia ruckeri vaccination. Raida MK, Buchmann K. Dis Aquat Organ. 2007 Aug 13;77(1):41‐52.


Søren Skov

Position Associate Professor in Immunology, KU‐LIFE

Previous positions and background Post doc in Seattle, Jeff Ledbetter lab. Assistant and Associate professor at KU (Health science and LIFE)

Brief description of research group and collaborators The Skov lab consists of 1 post doc, 3 PhD students, 4 Master students, 2 Bachelor students and 1 technician.

Collaborators:

Professor Bente Gammelgaard, KU‐Pharma (Pharmacology of Selenium compounds). Associate professor

Winnie Svendsen, Nanotech, DTU (Nanotechnology). Associate professor Lars Ellgaard KU‐Science (ER stress).

Heamatology department, Rigshospitalet (Primary cancer cells). Professor Hugh T. Reyburn, CNB, Madrid,

Spain (NKG2D / NKG2D‐ligand system in autoimmunity).

Scientific interests

Immunology, especially regulation of immunity during autoimmunity and cancer.

Assay development using Nanotechnoblogy, my lab is full partner in the EU project ENCABRA.

Technological/Analytical platform and key competencies

Cellular immunology: T and NK cell assays, Cell purification, Long term in vitro cultivation, Flow cytometry

Molecular immunology: Cloning and Transfection, Genechip, Promotor‐assay, siRNA array

In vivo immunology: In vivo assays of T and NK cell function

Selected papers 21: Skov S, Rieneck K, Bovin LF, Skak K, Tomra S, Michelsen BK and Ødum N. Histone deacetylase inhibitors: A new class of

immunosuppressors targeting a novel signal‐pathway essential for CD154 expression Blood, 101:1430, 2003.

22: Skov S. Therapeutic uses of depsipeptides and congeners thereof. Granted patent: US6548479, april 15, 2003.

25: Skov S, Pedersen MT, Andresen L, Thor Straten P, Woetmann A and Ødum N. Cancer cells become susceptible to NK

cell killing after exposure to Histone Deacetylase‐inhibitors due to GSK‐3 dependent expression of MICA/B. Cancer

Research, 65:11136, 2005.

27: Skov S. Use of depsipeptide and congeners thereof as immunosuppressants in preventing or treating rejection

following transplantation and for induction of apoptosis of activated CD4 and CD8 T‐cells. EPO Patent No. 07002930.1‐

2101, May 15, 2007.

28: Andresen L, Jensen H, Pedersen MT, Hansen KA and Skov S. Molecular regulation of MICA expression after HDAC‐

Inhibitor treatment of Jurkat T cells. J. Immunol., 179:8235, 2007

29: Andresen L, Hansen KA, Jensen H, Pedersen SF, Stougaard P, Hansen HR and Skov S. Propionic acid secreted from

Propionibacteria induce NKG2D‐ligand expression on human activated T lymphocytes and cancer cells. J. Immunol,

183:897, 2009.

30. Jensen H, Andresen L, Hansen KA and Skov S. Cell surface expression of Hsp70 on hematopoietic cancer cells after

inhibition of HDAC activity. J Leukocyte Biol, 86:923, 2009, 2009.

.

Part 2: Junior Faculty at Biomedicine (i.e. possible co-supervisors)

Alan Sten Nordal Mortensen Position/Principal investigator

Post.Doc./ Jens Lykkesfeldt

Previous positions and background 2010‐present Post.Doc., IVS, University of Copenhagen 2008‐2009 Regulatory affairs officer, Sun Chemical 2001‐2008 Specialist chemist, Chr. Hansen A/S 1997‐2001 Associate professor, Dept. Dairy and Food Science, KVL 1995‐1997 Assistant professor, Dept. Dairy and Food Science, KVL 1995 Ph.D. in chemistry (IR and Raman spectroscopy), University of Copenhagen

Scientific interests and current projects The role of tetrahydrobiopterin, a co‐factor of nitric oxide synthase, in endothelial dysfunction Project: Develop an analytical method for analysis of tetrahydrobiopterin in plasma. Development and validation of biomarkers of oxidative stress and damage.

Key competencies Analytical chemistry – spectroscopy, HPLC Molecular toxicology

My 3 best papers A. Mortensen and L. H. Skibsted: "Real Time Detection of Reactions between Radicals of Lycopene and

Tocopherol Homologues", Free Rad. Res. 27, 229‐234 (1997) A. Mortensen: "Analysis of a Complex Mixture of Carotenes from Oil Palm (Elaeis guineensis) Fruit Extract",

Food Res. Intern. 38, 847‐853 (2005) A. Mortensen and A. Geppel: "HPLC‐MS Analysis of the Green Food Colorant Sodium Copper Chlorophyllin",

Innov. Food Res. Emerg. Technol. 8, 419‐425 (2007)

Anja Schmidt‐Christensen

Position/Principal investigator Postdoc /Dan Holmberg

Previous positions and background -Postdoc at KU‐LIFE, Center of Infection and Inflammation Research (CIIR)

‐PhD student in Molecular Parasitology, Bernhard‐Nocht‐Institute for Tropical Medicine in Hamburg, liver‐stage malaria

Scientific interests and current projects Autoimmunity, study of complex biological interactions Current Project: Visualizing the autoimmune process of type‐1 diabetes in the anterior chamber of the NOD mouse

Key competencies Confocal and live‐cell imaging, Immunohistochemistry, OPT Common methods in molecular biology

My 3 best papers Putrianti ED and Schmidt‐Christensen A, Arnold I, Heussler VT, Matuschewski K, Silvie O (2010): The Plasmodium serine‐type SERA proteases display distinct expression patterns and non‐essential in vivo roles during life cycle progression of the malaria parasite. Cell Microbiol 12(6), 725‐39. Schmidt‐Christensen A, Sturm A, Horstmann S, Heussler VT (2008): Expression and processing of Plasmodium berghei SERA3 during liver stages. Cell Microbiol, 10(8): 1723‐1734. Van de Sand C, Horstmann S, Schmidt A, Sturm A, Bolte S, Krueger A, Lütgehetmann M, Pollok JM, Libert C, Heussler VT (2005): The liver stage of Plasmodium berghei inhibits host cell apoptosis.

Mol Microbiol, 58(3):731‐42.

Anne‐Marie Wegener

Position/Principal investigator

Post doc/Dan Holmberg

Previous positions and background

Research scientist, Pharmexa

Production manager, DanDrit Biotech

Post doc at Institute for experimental Immunology, Panum

Ph.D. student at Centre d’Immunologie de Marseille‐Luminy, France

Master in Biochemistry/Immunology, University of Copenhagen

Scientific interests and current projects I am interested in understanding the molecular mechanisms of T cell activation in relation to autoimmunity. The diabetes susceptibility locus denoted Idd28 (also called Ctex) in the NOD (Non Obese Diabetic) mouse is associated with a less activated phenotype of T cells (ref.: Lundholm, M. et al, Journal of Immunology, 2010, 184: 5537‐44). The NOD allele of the CD3‐zeta is a candidate gene for this phenotype. My current project is to understand the molecular mechanism responsible for the phenotype of this genotype.

Key competencies T cell biology and signaling, immunological tolerance/break of tolerance in autoimmunity and cancer.

My 3 best papers Wegener AM, Letourneur F, Hoeveler A, Brocker T, Luton F & Malissen B (1992) "The T cell receptor/CD3 complex is composed of two autonomous transducing modules". Cell, 68:83‐95. Wegener AM & Malissen B (1994) "Analysis of the (YXXL/I)2 signalling motifs found in the cytoplasmic segment of the mouse CD3‐ζ chain". Adv. Exp. Med. Biol., 365:45‐51. Steinaa L, Rasmussen PB, Wegener AM, Sonderbye L, Leach DR, Rygaard J, Mouritsen S & Gautam AM (2005) ”Linked foreign T cell help activates self‐reactive cytotoxic T lymphocytes (CTL) and inhibits tumour growth”. Journal of Immunology, 175:329‐34.

Lars Andresen

Position/Principal investigator Postdoc in the lab of Søren Skov

Previous positions and background I started working as a master student with HIV infected individuals analyzing signal transduction pathways in white blood cells from these patients. As a PhD student I studied transcriptional activity in the colonic epithelium from patients with chronic intestinal diseases such as colllagenous colitis and ulcerative colitis. After defending my phd thesis I started as a postdoc working with regulation of the NKG2D/NKG2D‐ligand system in cancer in the lab of Søren Skov, Univ. of Copenhagen. From spring 2009 I have started collaboration with Søren Skov and Winnie E. Svendsen from DTU‐Nanotech on implementing various immunological detection techniques into a nanotechnological platform and microfluidic systems.

Scientific interests and current projects I am very interested in identifying the molecular events that regulate the NKG2D ligands in health and disease. I have worked on that project since 2006 when I started as a postdoc in the Skov lab. I am also collaborating with DTU‐Nanotech on a project that aims at developing a sensor that can be used to electronically detect specific antibodies in the blood.

Key competencies Molecular Immunology, Molecular Biology, Technical skilled

My 3 best papers

Andresen L, Jørgensen VL, Perner A, Hansen A, Eugen‐Olsen J, Rask‐Madsen J. Activation of nuclear factor‐kappa B in colonic mucosa from patients with collagenous and ulcerative colitis. Gut 2005; 54:503‐9. Andresen L., H. Jensen, M. T. Pedersen, K.A. Hansen, and S. Skov. Molecular Regulation of MHC Class I Chain‐Related Protein A Expression after HDAC‐Inhibitor Treatment of Jurkat T Cells. Journal of Immunology 2007; 179:8235‐42. Andresen L., Hansen K.A., Jensen H., Pedersen S.T., Stougaard P., Hansen H.R., Skov S. Propionic acid secreted from Propionibacteria induce NKG2D‐ligand expression on human activated T lymphocytes and cancer cells. Journal of Immunology 2009; 183: 897‐906.

Malene Muusfeldt Birck

Position/Principal investigator Post doc / Jens Lykkesfeldt

Previous positions and background

DVM 2004

Veterinarian at the Department of Small Animal Clinical Sciences, LIFE, KU (2004‐2006)

PhD at Department of Veterinary Disease Biology, Section of Biomedicine, LIFE, KU (2006‐2011)

Scientific interests and current projects

Animal models of cardiovascular disease (atherosclerosis) and other life style disorders

Pig models of atherosclerosis (diet‐induced and infection/inflammatory‐induced)

Gastric bypass model using obese Göttingen minipigs

Key competencies

Animal models, surgery, anesthesia, histology, electron microscopy, stereology, endothelial function testing

My 3 best papers Malene M. Birck1,*, Erkki Pesonen2, Michal Odermarsky2, Axel K. Hansen1, Kenneth Persson3, Henriette Frikke‐Schmidt1, Peter M. H. Heegaard4, Petru Liuba2 Infection‐Induced Coronary Dysfunction and Systemic Inflammation in Piglets Are Dampened in Hypercholesterolemic Milieu. The American Journal of Physiology ‐ Heart and Circulatory Physiology (in press) Malene M. Birck1, T Iburg2, M Schmidt3, P T Sangild4 and A K Hansen.1 A novel method for trans‐uterine identification of piglets. Laboratory Animals (2008), Vol 42, 331‐337


Pernille Yde Tveden‐Nyborg Position/Principal investigator

Assistant Professor (experimental pharmacology)/ Jens Lykkesfeldt

Previous positions and background Assistant professor in veterinary biochemistry (KU‐Life), PhD in cell biology (KU‐Life) General veterinary practitioner Master of Veterinary Science (KU‐Life)

Scientific interests and current projects From an overall view point, my scientific interests lie within the investigation of biological mechanisms in animal models in order to improve human welfare. I am particularly interested in exploring pathways affected by vitamin C and its putative role as a key factor during neuronal function and development. This is currently reflected by my primary project concerning the potential effects of vitamin C during neuronal development, applying the guinea pig as in vivo model for human infants. However, other areas of investigating the effects of sufficient and insufficient vitamin C homeostasis and redox imbalances in the body, e.g. during ageing, are also within my line of interest.

Key competencies Molecular Biology

Gene expression analysis

Immunohistochemistry

Transmission Electron Microscopy

In vivo pharmacology

In vivo animal models (PK/PD)

My 3 best papers

Tveden‐Nyborg, P; Johansen, LK; Raida, Z; Villumsen, CK; Larsen, JO and Lykkesfeldt, J.Vitamin C deficiency in early post natal life impairs spatial memory and reduces the number of hippocampal neurons in guinea pigs (2009), Am J Clin Nutr, 90: 540‐546.

Tveden‐Nyborg, P and Lykkesfeldt, J Does vitamin C deficiency result in impaired brain development in infants (2009), Redox Rep, 14: 2‐6.

Tveden‐Nyborg, P; Alexopulous, NI; Cooney, MA; French, AJ; Holland, MK; Thomsen, PD and D’Cruz, NT Analysis of the expression of putatively imprinted genes in bovine peri‐implantation embryos (2008), Therio, 70: 1119‐1128;

Shashank Gupta

Position/Principal investigator Postdoc /Dan Holmberg

Previous positions and background Postdoc at Max Delbrueck Centre for Molecular Medicine Berlin, Germany Ph.D. student at Max Delbrueck Centre for Molecular Medicine/Humboldt University Berlin, Germany Research Scientist (Pharmacology) at New Drug Discovery Research Division of Ranbaxy Research Labs Master in Pharmacy (Pharmaceutical biotechnology), Birla Institute Of Technology, India

Scientific interests and current projects I am interested in understanding the cellular and molecular mechanisms involved in the progression of autoimmune inflammation in Multiple Sclerosis (MS) and Type 1 Diabetes (T1D). I am currently working on following projects:

a) Investigating the role of plasmacytoid dendritic cells (pDC) in mouse models of T1D and MS by using knock out mouse system (ref: Cisse,B., Cell 2008;135:37‐48).

b) Using Optical Projection Tomography (OPT) I am working to describe the natural development of EAE, by visualizing neuroinflammation, neurodegeneration and demyelination in CNS tissue from different rodent models of MS (ref: Alanentalo,T.,Diabetes. 2010:1756‐64. 2010).

c)

Key competencies Autoimmunity, Immunomodulation, antigen presentation, MHC/peptide interaction, DC Biology

My 3 best papers Gupta S, Höpner,S.,Rupp,B.,Günther,S.,Dickhaut,K.,Agarwal,N., Cardoso,MC., Kühne,R., Wiesmüller,KH.,Jung ,G., Falk,K., Rötzschke,O., Anchor side chains of short peptide fragments trigger ligand‐exchange of class II MHC molecules. PLoS ONE 2008; 3(3): e1814.

Hoepner,S.,Dickhaut,K.,Hofstaetter,M.,Kraemer,H.,Ruckerl,D.,Soederhall,JA.,Gupta,S.,Esteban,VM.,Kuehne,H.,Freund,C.,Jung,G.,Falk,K.,Roetzschke,O., Small organic compounds enhance antigen loading of class II major histocompatibility complexproteins by targeting the polymorphic P1 pocket. The Journal of Biological Chemistry. 2006 Dec 15; 281 (50): 38535‐42

Chopra,P.,Kulkarni,O.,Gupta,S.,Bajpai,M.,Banerjee,M.,Kanoje,V.,Chaira,T.,Shirumalla,R.,Verma, AK, Palle,VP.,Ramanathan,VK.,Sharma,G.,Ray,A., Pharmacological profile of AW‐814141, a potent, orally active inhibitor of p38 MAP kinase. International Immunopharmacology (2010), 10(4): 467‐73.

Contact information

Name KU‐LIFE Address Email Office phone

Alan Mortensen Ridebanevej 9, 1.sal [email protected] +45 35333165

Alf Skovgaard Stigbøjlen 7 [email protected] +45 35332703

Anja Schmidt‐Christensen Ridebanevej 9, 1.sal [email protected] +45 35331582

Anne‐Maria Wegener Ridebanevej 9, 1.sal [email protected] +45 35331579

Axel Kornerup Hansen Thorvaldsensvej 57 [email protected] +45 35332726

Bent Aasted Stigbøjlen 7 [email protected] +45 35332727

Christian Friis Ridebanevej 9, 2.sal [email protected] +45 35333161

Dan Holmberg Ridebaneven 9, 1.sal [email protected] +45 35332396

Dorte Bratbo Sørensen Thorvaldsensvej 57 [email protected] +45 35332724

Jens Lykkesfeldt Ridebanevej 9, 1. sal [email protected] +45 35333163

Kurt Buchmann Stigbøjlen 7 [email protected] +45 35332700

Lars Andresen Stigbøjlen 7 [email protected] +45 35333124

Lisbeth Høier Olsen Ridebanevej 9, 1.sal [email protected] +45 35333175

Malene Muusfeldt Birck Ridebanevej 9, 1.sal [email protected] +45 35333167

Martin Kristian Raida Stigbøjlen 7 [email protected] +45 35332701

Pernille Tveden‐Nyborg Ridebanevej 9, 1.sal [email protected] +45 35333167

Shashank Gupta Ridebanevej 9, 1.sal [email protected] +45 35331579

Søren Skov Stigbøjlen 7 [email protected] +45 35333126

scientist catalogue 2011 final - ku

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