restriction map: 1 psti psti + ccoiv 700030007000 4000 psti ccoi 7kbp 1 cut 2 cuts 3kbp 4kbp
TRANSCRIPT
Restriction Map: 1
PstIPstI +CcoIV
CcoIV
7000 3000 7000
4000
PstI
CcoI
7Kbp
1 cut 1 cut2 cuts2 cuts
3Kbp3Kbp
4Kbp4Kbp
Restriction Map: 2
EcoRiEcoRI +BstEII
BstEII
200 100 100
800 200 900
700
1,000
1 cut 1 cut2 cuts2 cuts
EcoRI200 800
BstEII
100900
100 900
100900
Restriction Map: 3
XhoIXhoI + FstII
FstII
1,000 1,000 3,000
5,000 2,000 14,000
11,000 3,000
11,000
FstII
17,000
14,000 3,000
2 cut 3cut3cut 1 cut
XhoIXhoI
5,0001,00011,0002,0002,000 3,0003,000
oror
Restriction Map 1Construct a plasmid restriction map of the
following digest. Include cuts and fragment sizes.
EcoREcoRII EcoRI + HindHindIIIIII
HindIII
7,0007,000 6,000 10,00010,000
3,0003,000 3,000
1,000HindHindIIIIII
EcoREcoRII
10K
1K
6K
EcoREcoRI = 2 sitesI = 2 sites
HindHindIII = 1 siteIII = 1 site
EcoREcoRII
3k3k
10k10k10k10k
10k10k
10k10k
7k7k1.) Linear or Plasmid ?2.) What’s the Size?3.) How many cuts?
4.) Easy Cuts First!5.) Find relationships!
6.) Rotate
=10K=10K= 3 sites= 3 sites
=HindIII=HindIII
Linear: Fragments = cuts + 1Plasmid : Fragments = cuts
Restriction Map 2Construct a plasmid restriction map of the following
digest. Include cuts and fragment sizes.
HhaII HhaII + XhoI
XhoI
6,000 4,000 6,000
2,000 HhaII
XhoI
6K 2K4K
HhaHhaII = 1 siteII = 1 site
XhoXhoI = 1 siteI = 1 site
Restriction Map 3Restriction Map 3A RIVER runs from Stanton to Fredericksburg. Two Cabins are located on this stretch of river. The Teelin’s cabin and the Larson’s cabin are 5 km apart. The Larson’s cabin is 8 km from Stanton while the Teelin’s cabin is 11 km from Fredericksburg.
Using the data, make a mapmake a map of the river and the cabins located along it. Use a horizontal free line to represent the river and symbols to represent the cabins. Label the symbols and intervals between symbols with the measurements of distance between the cabins.
– How far is the Teelin’s cabin to Stanton?How far is the Teelin’s cabin to Stanton?– How far is the Larson’s cabin to Fredericksburg?How far is the Larson’s cabin to Fredericksburg?– Is there an alternate Map?Is there an alternate Map?
Stan
ton
Stan
ton
Larso
n’s
Larso
n’s
Teelin
’sT
eelin’s
Fred
ericksbu
rgF
redericksb
urg
8 km8 km 5 km5 km 11 km11 km
Stan
ton
Stan
ton
Larso
n’s
Larso
n’s
Teelin
’sT
eelin’s
Fred
ericksbu
rgF
redericksb
urg
8 km8 km 11 km11 km
5 km5 km
How far is the Teelin’s cabin to Stanton? How far is the Larson’s cabin to Fredericksburg?Is there an alternate Map?
13K13Kmm 16K16K
mmYou Betcha , See You Betcha , See Below!Below!
Now how far is the Teelin’s cabin to Stanton? Now how far is the Larson’s cabin to Fredericksburg?
3Km3Km6Km6Km
Restriction Map 4Restriction Map 4There are towns along highway 28 running east to west. Using the data below, make a mapmake a map of highway 28 and the towns located along it. Use a free horizontal line to represent highway 28 and symbols to represent towns. Label the intervals between the towns with the measurements of distance.
•Freeville : Mt. Crumpit Freeville : Mt. Crumpit 160 km160 km•Mt. Crumpit : WhovilleMt. Crumpit : Whoville 200 km200 km•Waterville : FreevilleWaterville : Freeville 120 km120 km•Whoville : WatervilleWhoville : Waterville 240 km240 km•Mt. Crumpit : WatervilleMt. Crumpit : Waterville 40 km 40 km•Freeville hunters get to start each hunting day 8 Freeville hunters get to start each hunting day 8 min. sooner than Waterville hunters.min. sooner than Waterville hunters.
EastEastWestWest
120K120K40K40K200K200K
Fre
evill
eF
reev
ille
Wat
ervi
lleW
ater
ville
Mt.
Cru
mpi
tM
t. C
rum
pit
Who
ville
Who
ville
Restriction Map 5Construct a Linear restriction map of the following
digest. Include cuts and fragment sizes.SamIII SamIII + TrpII
TrpII
13,00013,000 10,000 17,00017,000
9,0009,000 9,000 10,00010,000
5,0005,000 5,000
3,000
TrpIISamIIISamIII10K
10K10K17K17K
3K5K5K9K9K
27K27K
27K27K
27K27K
SamSamIII = 2 sitesIII = 2 sites
TrpTrpII = 1 siteII = 1 site
13K13K
Restriction Map 6Construct a plasmid restriction map of the following
digest. Include cuts and fragment sizes.
CabCabIIII CabII + MtGMtGII
MtGI
6,0006,000 4,500 8,0008,000
3,0003,000 2,500 2,0002,000
1,0001,000 1,500
1,000
10K10K
500
8K8K
CabCabII = 3 sitesII = 3 sites
MtGMtGI = 2 siteI = 2 site
500
MtGMtGII
MtGMtGII2K2K10K10K
10K10K
10K10K
CabCabIIII1,500
CabCabIIIICabCabIIII
2,500
1,000
4,500
Restriction Map 73. There is a trail that runs along a mountain ridge in eastern Vermont
from James Camp to Camp Five. Using a horizontal line to represent the trail and dots to represent climbing features. Label the intervals between climbs with the measured distance.
• Blood Lake : Washington Peak 3 km• Washington Peak : Misery Rock 4.5 km• Hangman's Cliff : One Way Jacks Trail 1 km• James Camp : Jackson's Thrill 2 km• Hangman’s Cliff : Misery Rock 9.5 km• Washington’s Peak : Jackson’s Thrill 4 km• Jackson’s Thrill : Misery Rock .5 km• One Way Jacks Trail : Jackson’s Thrill 10 km• Blood Lake : Camp Five 6.5 km
• Jackson’s Thrill should be climbed in the sun before the sun sets behind Washington’s Peak
James C
amp
James C
amp
Misery R
ock
Misery R
ock
Jackson
’s Th
rillJackso
n’s T
hrill
Wash
ing
ton
’s Peak
Wash
ing
ton
’s Peak
Blo
od
Lake
Blo
od
Lake
Han
gm
an’s C
liffH
ang
man
’s Cliff
On
e Way Jack’s T
railO
ne W
ay Jack’s Trail
Cam
p V
Cam
p V
1K1K 3.5K3.5K2K2K3K3K4K4K.5.5KK
1.5K1.5K
Problem set 1: #1
EcoRIEcoRIEcoRI + BamHI BamHIBamHI
10,000 8,000 10,000
2,000
10K10K
EcoRIEcoRI
BamHIBamHI
2K
8K
Problem set 1: #2
EcoRIEcoRIEcoRI + BamHI BamHIBamHI
8,000 6,000 12,000
4,000 4,000
2,00012K12K
EcoRIEcoRIBamHIBamHI
2K
4K
EcoRIEcoRI
6K
Problem set 1: #3
EcoRIEcoRIEcoRI + BamHI BamHIBamHI
8,000 6,500 6,500
4,000 4,000 5,500
1,000
500 12K12K
EcoRIEcoRI
BamHIBamHI
1K
6.5KEcoRIEcoRI
4K
500
BamHIBamHI
Problem set 1: #4ClaIClaI ClaIClaI +
HindIIIHindIII HindIIIHindIII
11,000 8,000 14,000
8,000 7,000 7,000
2,000 4,000
2,000
Cla
IC
laI
HindIIIHindIII
Cla
IC
laI
Hin
dIII
Hin
dIII
80008000 20002000 40004000 70007000
= 1site= 1site
ClaIClaI =2 sites=2 sites
Problem set 1: #5HindIIIHindIII
HindIII HindIII +
BamHIBamHIBamHIBamHI
15,000 15,000 18,000
10,000 7,000 13,000
6,000 6,000
3,000
HindIIIHindIII
Hin
dIII
Hin
dIII
= 2 sites= 2 sites
BamHIBamHI =1 site=1 site
1500015000 30003000 70007000 60006000B
amH
IB
amH
I
Hin
dIII
Hin
dIII
Problem set 1: #6PvulPvul SmalSmal +
PvulPvul SmalSmal
8,800 8,000 11,800
8,000 5,500 8,700
6,700 4,500 7300
6000 3,800 4200
5500 3200 3000
3000
2800
2200
2000
SmaISmaI
Sm
aIS
maI
= 4 sites= 4 sites
PvuIPvuI = 4 sites= 4 sites
Pvu
IP
vuI
3200320055005500 28002800
Pvu
IP
vuI
Sm
aIS
maI
45004500 20002000P
vuI
Pvu
I22002200 30003000
Sm
aIS
maI
Sm
aIS
maI
38003800
Pvu
IP
vuI
80008000
Problem set 2: #7EcoRIEcoRI EcoRIEcoRI +
BamHIBamHI BamHIBamHI
6,000 4,500 8,000
3,000 2,500 2,000
1,000 1,500
500
BamHIBamHI1,0001,000
= 2site= 2site
EcoRIEcoRI =3 sites=3 sites
BamHIBamHI
BamHIBamHI
EcoRIEcoRIEcoRIEcoRI
EcoRIEcoRI
2,5002,500
500500
1,5001,500
4,5004,500
Problem set 2: #8
EcoRIEcoRI + BamHI
BamHIBamHI+ HindIII
HindIIIHindIII + EcoRI
18,000 9,000 11,500 9,000 16,300 14,500
5,000 8,000 9,000 8,700 8,700 5,000
4,000 5,000 8,000 4,500 3,500 3,500
1,500 4,000 3,500 2,200
1,500 2,800 1,800
1,000 1,500E
coR
IE
coR
I
Eco
RI
Eco
RI
Hin
dIII
Hin
dIII
Bam
HI
Bam
HI
3,5003,500 4,5004,500 9,0009,000
Bam
HI
Bam
HI
1,0001,000H
indI
IIH
indI
II1,8001,800 2,2002,200 5,0005,000 1,5001,500
Eco
RI
Eco
RI
8,0008,000 4,0004,000
Problem set 2: #9EcoRIEcoRI EcoRIEcoRI +
BamHIBamHI BamHIBamHI
9,000 8,000 11,000
6,000 6,000 7,000
3,000 3,000
1,000
Eco
RI
Eco
RI
BamHIBamHI
Eco
RI
Eco
RI
Bam
HI
Bam
HI
6,0006,000 1,0001,000 8,0008,000 3,0003,000
= 1site= 1site
EcoRIEcoRI =2 sites=2 sites
Problem set 2: #10
EcoRIEcoRIEcoRI EcoRI
+ BamHIBamHI
BamHIBamHI HindIIIHindIIIHindIII HindIII
+ EcoRIEcoRI
ClaI ClaI +
EcoRIEcoRIClaIClaI
14,000 9,000 17,000 12,000 11,000 14,000 19,000
9,000 8,000 6,000 11,000 9,000 5,000 4,000
6,000 3,000 4,000
Bam
HI
Bam
HI
Hin
dIII
Hin
dIII
Eco
RI
Eco
RI
Cla
IC
laI
6,0006,000 5,0005,000 3,0003,000 5,0005,000 4,0004,000
Quick Draw Δ (linear)
SnaTIISnaTII
SnaTIISnaTII+ BkaIBkaI
BkaIBkaI
16,000 15,000 18,000
15,000 13,000 13,000
3,000S
naT
IIS
naT
II
15,00015,000 3,0003,000 13,00013,000B
kaI
Bka
I
31,000
Quick Draw Σ (Plasmid)
TtHIITtHII TtHIITtHII + ZtoPIZtoPI ZtoPIZtoPI
8,500 8,000 8,500
500
TtHIITtHII ZtoPIZtoPI
8,0008,000
500500
8,5008,500
Quick Draw Ω (linear)
SpaZIIISpaZIII
SpaZIIISpaZIII+ HmmIHmmI
HmmIHmmI
115 100 130
40 30 15
15 10
10
Spa
ZII
IS
paZ
III
1010 3030 100100
Hm
mI
Hm
mI
Hm
mI
Hm
mI
1515
155