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 Entamoeba histolytica may be observed in thefeces in two morphology stages (necessary fordiagnostic importance) :

trophozoitecyst

This specimen are stained in Trichrome

Entamoeba histolytica 

Diagnosis : histolytica forms in feces, abscess

ulcer

 immunological reactions 

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Entamoeba histolytica

Vegetatif form  Size : 20-40 micron

One Entamoebicnucleus

Contain red bloodcells

Endoplasm isgranular

Ectoplasm acts as apseudopodia

nuclei

RBC

endoplasm

ectoplasma

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Entamoeba histolytica

Vegetatif form  Size : 20-40 micron

One Entamoebicnucleus

Contain red bloodcells

Endoplasm isgranular

Ectoplasm acts as apseudopodia

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Entamoeba histolytica

cyst form 

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Entamoeba histolytica

Cyst form

Size : 20-40 micron

our entamoebicnuclei !maturecyst"

Ectoplasm isgranular

nuclei

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Entamoeba coli vegetative form

Size : 15-30 micron

(rbc : 7 micron)

Entamoeba nucleus

acuole Endoplasm

Ectoplasm unclear

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 Entamoeba coli Vegetative form

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Entamoeba coli cyst form

Size : 15-!! micron

S"ape : rounded

Entamoebic nucleus

Contain : ! nuclei

#"e position o$

nucleus are

separated b% &acuole'lio'en

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Entamoeba coli mature cyst form

Size : 15-!! micron

S"ape : rounded

(o&al)

Entamoebic nucleus

Contain : nuclei

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Giardia lamblia may be obser#ed in thefeces in t$o morphology stages!necessary for diagnostic importance" :

trophozoite cyst

 %his specimen are stained in %richrome

Giardia lamblia 

Diagnosis : #egetati#e forms in diarrheic feces &

duodenal 'uid

 cyst forms in feces 

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Giardia lamblia

(egetati#e form  Size : )4 micron

 %he shape loo*s li*e +ambumonyet !bilateral

symmetrical"  %$o nuclei

One suc*er

 %$o a.ostyles

 %$o blepharoplasts and fourpairs of 'agella bet$eenthe anterior and posteriorlateral pairs !doesnt sho$"

 %$o parabasal bodies

nuclei

sucer

a*ost%les

+arabasal

bodies

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 Giardia lamblia

Cyst form

Ellipsoidal shape

Size : )0 - )4 micron  %$o / four nuclei

Cyst $all

 %$o blepharoplasts

 %$o parabasal bodies

nuclei

+arabasal

bodiesC%st ,all

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 Balantidium coli

Vegetative formOval shape

Size : ± 60 micron

Nuclei :Macronucleus

Micronucleus(isappear!

Vacuole

Cilia can "e seenvaguely

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 Balantidium coli

Cyst form

Oval shape

Size : ± 60 micron

Nuclei :Macronucleus

Cilia cant "e seen

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Cryptosporidium parvum

oocyst very small 10 micron

  (4-5 micrometers in

diameter)

!cystment

"at#re oocysts contain 4

sporozooites

$ocysts appear as bright

pin% to red containing some dar%

gran#les

oocyst

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Cyclospora cayetanensis

oocyst &ize : 15 ' 0 mi%ron

Transparent cyst wall

"at#re oocysts contain 4

sporozooites

$ocysts appear as bright pin%

to red

alled *giantryptosporidi#m+

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Cyclospora cayetanensis

oocyst

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Isospora belli 

oocyst Oval shape red color 

 size ,5 -

 oocyst contain , sporocyst

 sporocyst contain

4 sporozoit

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Blastocystis hominis

Oval

Size : 5-30 micron

Two – four peripher

nuclei

Cyst wall

nuclei

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