parasitic protozoa
DESCRIPTION
Parasitic Protozoa. Slackers Facts by Mike Ori. Disclaimer. The information represents my understanding only so errors and omissions are probably rampant. It has not been vetted or reviewed by faculty. The source is our class notes. - PowerPoint PPT PresentationTRANSCRIPT
Parasitic Protozoa
Slackers Facts by Mike Ori
Disclaimer
The information represents my understanding only so errors and omissions are probably rampant. It has not been vetted or reviewed by faculty. The source is our class notes.
The document can mostly be used forward and backward. I tried to mark questionable stuff with (?).
If you want it to look pretty, steal some crayons and go to town.
Finally…
If you’re a gunner, buck up and do your own work.
What are the three related sporozoans?
ToxoplasmosisCryptosporidium
Plasmodia
What is the definitive host for toxoplasmosis?
What are the routes of acquisition for toxoplasmosis?
1. Ingestion of undercooked meat especially lamb and pork (vive la France)
2. Cat feces3. Vertical
Explain when toxo is a concern during transplant
When a seropositive donor heart is transplanted into a seronegative recipient
What results from post transplant infection
Myocarditis
What sx would you expect to see in aids pt
Ring enhancing lesions in the brain
Since toxo increases in aids pt, what type of immunity is likely to play a role in toxo
control?
Cell mediated immunity
What is the source of toxo in aids pt
Usually reactivated encysted (bradyzoites) organisms
What is a common source of diarrhea in PT with low CD4+ counts?
Cryptosporidium
Describe the disease course in immunocompetent and immunocompromised
pt
Competent: incubation < 2 weeks with 1 week sx.
Immunocompromised: long term diarrhea
What is the treatment for cryptosporidium in immunocompromised pt
Raise the CD4 count.
What organism could cryptosporidium be mistaken for in “regular stains”?
Candida
How do you differentiate cryptosporidium from candida
Crypto is acid fast, Candida is not
Why is crypto hard to treat
The organisms invades the ciliated surface and sits in an intracellular but extracytoplasmic location. This limits access to antimicrobial
agents.
Describe Girardia morphology
Flagellated pear shaped organisms with two nuclei and a concave sucking disk
Describe Giardia epidemiology
Outdoorsy people that drink from streams without properly treating the water.
What are girardia’s sx?
Most are asymptomatic. Those that have sx experience loose foul smelling stools,
abdominal ramps, bloating, weight loss.
Explain how Girardia attaches to the host
It uses its ventral sucking disk for attachment.
How many nuclei does the encysted Giarardia have
Quattro
Which test is more sensitive, O and P or fluorescent antibodies, or ELISA
O and P is not very sensitive especially if the small intestine is colonized.
O and P is repeated three.
Three tests are typically done. FA and ELISA are much more sensitive and usually yield results
in one test.
What is the string test?
PT’s with small intestinal disease swallow the end of a string overnight in an effort to trap
organisms in the string for detection.
What is metabolically unique about anaerobic protozoans?
They do not synthesize nucleotides
Name three anaerobic protozoans
Girardia, Entamoeba, Trichomonas
Describe the SX with Entamoeba infection
Bloody diarrhea
When is Entamoeba infection not a clinical problem
Entamoeba dispar is a frequent colonizer but is not known to cause disease. E. histolytica is
only a problem if its invasive.
What is the evidence for invasion with E. histolytica?
RBC’s ingestion
How are E. dispar and E. histolytica differentiated?
By PCR
What colon morphology is indicative of amoebiasis
Inverted flask shaped ulcers.
What is the reservoir for Entamoeba
E. Histolytica = human
What other organs are involved with Entamoeba and what are the findings
Liver can be involved with anchovy paste filled cysts. Organisms will be on the periphery of
the cyst.
Describe the sx for trichomonas vaginalis in men and women
Men are usually asymptomaticWomen will usually have a foul smelling green
discharge
How is Trichmonas vaginalis transmitted?
By sexual contact
Women
How is enamoeba transmitted?
Food > water
How is Girardia transmitted?
Water > food
What is the reservoir for Girardia in the wild
Reservoir = beaver = reservoir builder
What disease does plasmodium cause
Malaria
Name the plasmodium species
P. vivaxP. ovale
P. malariaeP. falciparum
Which species is associated with the most deaths?
P. falciparum
What is the route of transmission for Plasmodium
Anopheles
What is the definitive host for malaria
Mosquitoes
What is injected into humans by said mosquito?
A malaria sporozoite
Where do the sporozoites go
The liver
What is a schizont
The replicating malarial mass in a hepatocyte or RBC
What is a malaria trophozoite?
Something less than a schizont but more than a merizoite.
Why does malaria fever come in 2-3 day intervals?
The release of the merizoites from RBC’s is fairly well coordinated and occurs in a large burst.
This exposes the organism to the immune system and causes a fever. The merizoites
then go on to infect more RBC’s thus causing a cycle.
When might the fever cycle degenerate?
P. falciparum tends to degenerate. Multiple infection cycles also tend to degenerate
What are the unique histological features of P. falciparum?
Schizont forms tend to lodge in the microvasculature and so are not seen on
routing blood smears. In addition, more ring forms and double infected RBC’s are seen in P.
falciparum
What is sequestration
The action of retaining shizonts in the microvasculature. This prevents removal by
the spleen.
What is unique about the life cycle of P. vivax and P. ovale?
They can have latent hepatic stages that can release merizoits months to years later. This is
in contrast to P. falciparum and P. malariae which release all their merizoites from the
liver in a single go.
Which blood cells does P. vivax invade?
It only invades reticulocytes.
What is the role of receptors in malarial disease
Merizoites interact with species specific receptors on the RBC surface. For instance P. vivax required Duffy and reticulocyte receptor
Where are receptors located on merizoites
In a special organelle called the rhoptry
Explain the high incidence of sickle cell trait and the low incidence of Duffy in west Africa
Sickle cell trait is protective for severe malaria. Absence of Duffy prevents P. vivax invasion of reticulocytes. Note there are other examples
in the notes.
Explain the belief that the immune response to malaria is incomplete
Mortality is highest in naïve pt (children mostly in endemic areas). Adults can carry a high
parasitemia with little sx and thus seem to be protected from severe disease.
What are the run of the mill sx for malaria
High fever (40-41c)Chills
Headache
What is the typical causative species for severe malaria?
P falciparum
What are the sx of severe malaria
Cerebral malaria – seizures and coma due to cerebral sequestration
Severe anemia (Hgb < 5) – falciparum can have very high infectivity
General illness – prostration, hypoglycemia, lactic acidosis, ARDS
What is chronic malaria
Continuous parasitemia in contrast to recurring parasitemia
What is the causitive agent for chronic malaria
P. falciparum and P. vivax
What are the methods to prevent malaria
RepellentsMosquito netting
ClothingProphylactic pharmacology
List the malaria time course
Prepatent (hepatic) phase – 2 weeksRed cell phase – 2-3 days
What is the vector for leishmania
Sand flies
What is an amastigote and a promastigote?
A mastigote = amotile (I.e. no flagella)Promastigote = pretty good swimmer
(flagellated)
What cell is invaded by promastigotes
Macrophages
What is the result for the macrophage?
Eventual lysis of the cell due to replication
What are the three types of leishmaniasis?
CutaneousMucocutaneous
VisceralDiffuse cutaneous
Describe cutaneous leishmaniasis
Ulcerative lesions that eventually heal with no sequelae
Describe mucocutaneous leishmaniasis
Cutaneous lesions heal but later destruction of nasal and mucosal tissue occurs
Describe visceral leishmaniasis
Invasion of the liver and other visceral leading to death
Describe diffuse cutaneous leishmaniasis
Treatment resistant non-ulcerative lesions disseminated over the skin surface. Very tx
resistant.
Describe the African Trypanosoma brucei species ranges
Roughly divided into east and west african
What is the vector from Trypanosoma brucei
The tsetse fly
Describe the hosts for both species
West = humanEast = big game animals with humans as
incidental
What are the stages of african trypanosomal disease
1. Bite/chancre2. Hemolymphatic – Lymphadenopathy with
relapsing fever3. Meningoencephalitis – somnolence, coma,
death
Why does the immune system fail to clear trypanosomes
The organisms express variant surface glycoproteins that cause antigenic variation
Describe the progression of T.b rhodesiense and T.b. ghambiense
Tbr is east african and is much more acute. Death occurs in months. Tbg is west african
and is less acute. Death occurs on years
How is tx complicated with CNS diease
Tx of CNS disease relies on arsenic containing compounds which are toxic to the host.
What is the vector for Trypanosoma cruzi
The reduviid bug
How is T. cruzi spread?
The reduviid bug bites a victim causing a pruritis. The bug also deposits trypanosome laden
feces near the bite. The host then scratches the bite, mechanically introducing the
organism. The organism can also pass directly across mucosal surfaces.
What is unique about the epithelial invasion of T. cruzi
It invades epithelial cells at point of cell to cell junction.
What are the sx of chagas disease
Nerve destructionMuscle inflammation and hypertrophy
Leading to dilated cardiomyopathy, arrhythmia, and GI tract dilation and esophageal
impairment (dysphagia, odynophagia)
What are the phases of chagas disease
AcuteIndeterminate
Chronic
What is Romana’s sign
Periorbital edema caused by T cruzi entry across the eye mucosa.
What is the cause of most deaths from chronic chagas disease?
Cardiac disease
What is meant by indeterminate disease
Chagas disease may enter a latent phase that can lead to acute flares, continued latency, or
chronicity. There is no way to predict the outcome.
Which phases of chagas disease are treatable?
Acute is treatable. Indeterminate and chronic do not benefit from treatment.