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    Plant Disease and ControlIntroduction to

    Abdul HafeezPlant PathologistDepartment of Agriculture Azad Kashmir

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    DiseaseDisease

    Series of responsesSeries of responses

    abnormalityabnormality

    lossloss

    microorganismsmicroorganisms EnvironmentalEnvironmental factorsfactors

    morphologymorphology

    physiophysiollogyogy

    quantquant ityity

    qualiqualityty

    PLANT DISEASE ?PLANT DISEASE ?

    WHAT ISWHAT IS

    (shape)

    (function)

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    Symptoms

    The external & internal alterationsof a plant as a result of a disease

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    Primary SymptomsDirect changes of tissues infected by

    pathogen

    leaf spot

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    Secondary Symptoms

    Indirect & subsequent changes of a distance

    Sclerotium w ilt

    from the site of the pathogen

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    Localized symptoms

    (in a particular part of the plant)

    leaf blight

    (death of cells in restricted areas)

    (death of cells: not restricted)

    leaf spot

    bacterial canker

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    (Death of cells and tissues)

    dry rot: fungi

    Rots

    soft rot: bacteria

    anthracnose

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    Hypertrophy & Hyperplasia

    root knot (gall)

    (overgrowth of tissue)

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    Systemic symptoms

    (spreading throughout the plant body)

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    Transformation of tissues/ organs

    phyllody

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    Stunting/Dwarfing

    (Reduced Growth)

    Leaf roll / leaf curl

    Dwarf

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    Changes in colorationChanges in coloration

    phosphorus deficiencyphosphorus deficiency

    white leaf of sugarcanewhite leaf of sugarcane

    mosaic

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    Symptoms with SignsSymptoms with Signs

    rustrust

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    smutsmut

    Symptoms withSymptoms withSignsSigns

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    downy mildew

    powdery mildewpowdery mildew

    Symptoms w ith SignsSymptoms w ith Signs

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    Naming of DiseaseNaming of Disease

    11.. With symptomWith symptom papayapapaya ringspotringspot

    Watermelon fruit rotWatermelon fruit rot

    22.. With signWith signrustrustsmutsmut

    powdery mildewpowdery mildew

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    1) Noninfectious/ abiotic agents- imbalance of nutrients

    - extreme temperature/moisture

    - Air & w ater pollutants, chemicals, etc.

    2) Infectious / biotic agents

    Fungi, bacteria, mollicutes,

    viruses & viroids, nematodes,

    protozoa

    Causal agentsCausal agents

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    Shapes & sizes of plant pathogens

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    Fungi

    bacteria

    mollicutes

    viruses

    nematodes

    viroids

    protozoa

    Parasitichigherplants

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    DiseaseDiseaseTriangleTriangle

    TiTi

    meme

    HostHost

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    Disease CycleDisease Cycle

    DisseminationDissemination

    InfectionInfection

    Monocyclic Disease

    Polycyclic Disease

    inoculationinoculation

    SymptomSymptom

    (1)

    (2)(4)

    (3)

    developmentdevelopment

    Reproduction

    (5)

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    Diagnostic TechniquesDiagnostic Techniques

    1. Visual inspection of symptoms

    2. Microscopic exam ination

    3. Isolation for pure culture of pathogenand Identification of pure culture

    4. Biochemical & physiological methods

    5. Nucleic acid-based methods(molecular methods)

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    disease

    insect

    Visual Inspection ofVisual Inspection ofSymptomsSymptoms

    Determine : diseaseVS insect

    insect

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    Determine : biotic agentVS abiotic agent

    abioticbiotic

    Visual Inspection of SymptomsVisual Inspection of Symptoms

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    Determine : Nutrient Deficiency VS Virus

    virusnutrient deficiency

    Visual Inspection of SymptomsVisual Inspection of Symptoms

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    Fungal Diseases

    Visual Inspection of SymptomsVisual Inspection of Symptoms

    Use hand-lens/ microscope

    - mycelium/ fruit ing body/

    spores

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    bacterial ooze

    mass of bacterial cells

    cut stem

    from xylem

    from cut leafspot

    Bacterial Diseases

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    Virus and Phytoplasma

    virus

    phytoplamainsect vector

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    Transmission test

    Indicator plant

    Serology

    Electron microscope

    Nucleic Acid, DNA

    Virus andVirus and PhytoplasmaPhytoplasma

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    Nematode Damage

    root knotroot knot

    (gall)(gall)

    Nematodes instained root

    Symptom , Populat ion density in soil & root

    poor growth

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    Principles of Plant Disease Control

    1. Reducing Initial Inoculum

    2. Reducing Rate of Development

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    Reducing Initial

    InoculumExclusion - no introduction ofpathogen

    Eradication-

    eliminate pathogeninoculum

    Therapy - eliminate pathogen from living host

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    Reducing Rate of Development(To Slow Rate of Increase)

    Host resistance - horizontal resistance

    Protection - protect the host from infection

    Avoidance - avoid contact of host and pathogenunder conditions favorable for

    disease

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    Reducing Rate of Development

    AvoidanceAvoid putting host & pathogen in contact with

    one another under a favorable environment

    A. Geographic Selection

    B. Site Selection

    C. Environmental Management

    D. Cultural Practices

    Planting/Harvest dates Short season cultivars Cultivation practices, plant spacing Irrigation management, ferti l ity management

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    Reducing Rate of Development

    ProtectionA. Biological controlAntagonistic organisms (Streptomyces, Bacil lus,

    Trichoderma, Paecil lomyces, Vertici l l ium)

    Cross protection (inoculate mild strain to induceresistance for virulent strain of pathogen)

    B. Chemical control

    Protectant, Eradicant Contact, systemic pesticides

    Fungicides, bactericides, nematicides

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    Integrated P lantDisease Management

    1. Manage the pathogen

    2. Manage the host

    3. Manage the environment

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    Integrated P lant DiseaseManagement

    1. Manage the pathogen

    - Exclusion of inoculum

    - Reduction of inoculum

    - Eradication of inoculum

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    Integrated P lant DiseaseManagement

    2. Manage the host

    - Induced resistance

    - Breeding for disease resistance

    - Protect ion

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    Integrated P lant Disease

    Management

    3. Manage the environment

    - Soil management

    - Crop management

    - Water management