pif supplement workflow benefits and performance data
TRANSCRIPT
1
PIF Supplement Workflow Benefits
and Performance Data
Bio-Rad Laboratories
Food for Thought Newsletter, June 2020
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Workflow Benefits
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A Fully Harmonized Solution
Matrices
Targets
Screening Confirm.
Harmonized Infant formula without probiotics Infant formula with probiotics Ingredients
Harmonized Salmonella Cronobacter
Harmonized iQ-Check Kits RAPID’media
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Benefits of 1:4 Dilution
3.75 kg / bag 2.25 kg / bag 1.5 kg / bag
375 g
1:10
375 g
1:4
375 g
1:6
Make your technicians happier with 1:4 dilution
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Workflow Benefits
Save Time
• Technician handling
Save Space
• Incubators
Save Money
• Less media
• Less energy
• Less waste treatment
Save Energy
• For heating media
Save Waste
• Less media
A small 1:4 dilution factor for big benefits
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Summary
PERFORMANCE
EASE OF USE
REASONABLE COST
Very low level of detection, even in
challenging conditions:
Large sample size
Stressed bacteria
Large sample size suitable method
No additional materials / technicians
Co-enrichment Salmonella & Cronobacter
Reagent cost
Consumable & material cost
Labor cost
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Performance Data
AFNOR Validation Data
RAPID’Sakazakii
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RAPID’Sakazakii AFNOR Performance Data
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RAPID’Sakazakii AFNOR Performance Data
Sensitivity Study
RLOD Study Better sensitivity
than the reference
method
No false
positive
results
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RAPID’Sakazakii AFNOR Performance Data
AFNOR certificate (avalaible) Validation Summary Report
(soon available)
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Performance Data
AFNOR Validation Data
iQ-Check Cronobacter
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iQ-Check Cronobacter AFNOR Performance Data
• AFNOR validation extension:
– 375 g sample size
– 1:4 dilution in BPW pre-warmed + PIF
Supplement
– 18–26 hr single enrichment @ 37 ± 1°C
– Free DNA Removal Solution (FDRS)
– APF Fast: “Crono Fast”
– 72 hr storage
• Matrices
– Infant formula and cereals w/wo
probiotics and ingredients
– Environmental samples
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Sensitivity Study
Category SE Alt % SE Ref % RT % FP %
1 – 30 g regrowing 94.6 100.0 96.7 8.7
2 – 375 g + PIF 100 73.5 86.4 0.0
3 – Environmental 100.0 100.0 100.0 0.0
iQ-Check Cronobacter AFNOR Performance Data
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RLOD
1 – 30 g regrowing
2 – 375 g + PIF
3 – Environmental
iQ-Check Cronobacter AFNOR Performance Data
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AFNOR certificate (avalaible) Validation Summary Report
(soon available)
iQ-Check Cronobacter AFNOR Performance Data
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Thank You