NGS needs for study capture

Frans P Ruzius

Hubrecht Institute

NGS

• Next Gen(eragion) Sequencing

• Deep Sequencing

– Small reads

• 50-500 bp

– Lots of reads

• 0.5 – 800 M

– Big reference

• Genome

– 3 Gb

Sequencing run

Experiment webpage

Sequencing run

Experiment webpage

Sequencing run

Mapping pipeline

Sequencing run – future planns

Submission website

Sequencing run

Need for sample tracking

Most heard questions in the lab

• Can this be solved by study capture?

– Is my data already analyzed?• What is already mapped and what not?

– Where can I find my data?• Where is the data stored?

– From which run was my library?

– What was the mappability?