mn products for spemn products for spe

MN

MN products for SPEMN products for SPE

Standard phases for solid phase extraction

201

C6H11 ec cyclohexyl silica, endcapped

base material silica, pore size 60 Å, particle size 45 µm,specific surface 500 m2/g, pH stability 2 – 8cyclohexyl phase, endcapped, carbon content 9 %

– alternative phase for the mid-polar range

recommended application: phenols from waterchloroanilines from waste wateranthelmintics from tissue

Application: comparison of different phases for phenol analysis

Compounds investigated: Phenol, 2,4-dinitrophenol, pen-tachlorophenol

Column types: CHROMABOND® C18 ec, 6 ml, 2000mg, Cat. No.730141CHROMABOND® C18, 6 ml, 2000 mg, Cat. No.730130CHROMABOND® C6H11 ec, 6 ml, 2000 mg, Cat. No.730469CHROMABOND® C18 HYDRA, 6 ml, 2000 mg, Cat. No.730301

Column conditioning: 10 ml acetone, 10 ml methanol, and 10 ml dist. water (pH 2)Sample application: aspirate the sample through the col-umn.Elution: 10 ml methanol

Ordering information

0

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40

60

80

100

phenol 2,4-dinitrophenol pentachlorophenol

CHROMABOND®

C18 ec C18 C6H11 ec C18 Hydra

Volume Adsorbent weight Pack of

CHROMABOND® C6H11 ec polypropylene columns500 mg 1000 mg

3 ml 730442 506 ml 730443 730444 30

CHROMABOND® C6H11 ec glass columns500 mg 1000 mg

3 ml 730442 G 506 ml 730443 G 730444 G 30

CHROMABOND® LV-C6H11 ec

100 mg 200 mg 500 mg

15 ml 732440 732441 732443 30

CHROMABOND® MULTI 96 C6H11 ec

96 x 25 mg 96 x 50 mg 96 x 100 mg

738440.025M 738440.050M 738440.100M 1

CHROMABOND® C6H11 ec adsorbent

730631 100 g

Other column sizes and sorbent weights on request · Take advantage of our SPE service on page 232

3021

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Australia & New Zealand: Winlab Pty Ltd www.winlab.com.au email: [email protected] or call +61 (0)7 3205 1209

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202

C6H5 phenyl silica

base material silica, pore size 60 Å, particle size 45 µm,specific surface 500 m2/g, pH stability 2 – 8phenyl phase, carbon content 8 %

– polarity similar to C8– in addition to hydrophobic interactions more selective

adsorption is possible by π-π interactions due to theelectron density of the phenyl ring

recommended application: aflatoxinscaffeinephenols


NO2 nitrophenyl silica

base material silica, pore size 60 Å, particle size 45 µm,specific surface 500 m2/g, pH stability 2 – 8nitrophenyl phase, carbon content 5.5 %

recommended application: aromatics



CHROMABOND® C6H5 polypropylene columns100 mg 200 mg 500 mg

1 ml 730083 1003 ml 730411 730084 50

CHROMABOND® C6H5 glass columns100 mg 500 mg

1 ml 730083 G 1003 ml 730084 G 50

CHROMAFIX® C6H5 cartridges0.4 ml / 200 mg 0.8 ml / 400 mg 1.8 ml / 900 mg

731810 731811 731812 50

CHROMABOND® MULTI 96 C6H5

96 x 25 mg 96 x 50 mg 96 x 100 mg

738025.025M 738025.050M 738025.100M 1

CHROMABOND® C6H5 adsorbent730606 100 g


CHROMABOND® NO2 polypropylene columns500 mg 1000 mg

3 ml 730143 506 ml 730142 30

CHROMABOND® NO2 glass columns500 mg

3 ml 730143 G 50

CHROMAFIX® NO2 cartridges

0.8 ml / 400 mg

731826 50

On request, CHROMABOND® columns are also available in special versions for lab and flash robots


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203

NH2 aminopropyl silica

base material silica, pore size 60 Å, particle size 45 µm,specific surface 500 m2/g, pH stability 2 – 8aminopropyl phase, carbon content 3.5 %

– polar, weak anion exchangerrecommended application: trace elementslipids

DMA dimethylaminopropyl silicabase material silica, pore size 60 Å, particle size 45 µm,specific surface 500 m2/g, pH stability 2 – 8dimethylaminopropyl phase, carbon content 3.5 %

– polar, weak anion exchangerrecommended application: similar to NH2 – slightly weaker anion exchanger



CHROMABOND® NH2 polypropylene columns100 mg 200 mg 500 mg 1000 mg

1 ml 730031 1003 ml 730413 730033 506 ml 730180 730626 30

CHROMABOND® NH2 glass columns100 mg 500 mg 1000 mg

1 ml 730031 G 1003 ml 730033 G 506 ml 730180 G 730626 G 30

CHROMABOND® LV-NH2

100 mg 500 mg

15 ml 732031 732033 30

CHROMAFIX® NH2 cartridges0.4 ml / 200 mg 0.8 ml / 400 mg 1.8 ml / 900 mg

731813 731814 731815 50

CHROMABOND® MULTI 96 NH2

96 x 25 mg 96 x 50 mg 96 x 100 mg

738031.025M 738031.050M 738031.100M 1

CHROMABOND® NH2 adsorbent730603 100 g

CHROMABOND® DMA polypropylene columns100 mg 500 mg

1 ml 730041 1003 ml 730043 50

CHROMABOND® DMA glass columns100 mg 500 mg

1 ml 730041 G 1003 ml 730043 G 50



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204

CN cyanopropyl silica

base material silica, pore size 60 Å, particle size 45 µm,specific surface 500 m2/g, pH stability 2 – 8cyanopropyl phase, carbon content 5.5 %

– polar to mid-polar– in addition to weak hydrophobic interactions selective in-

teractions are possible due to the high electron densityof the CN group

recommended application: cyclosporinscarbohydrates



CHROMABOND® CN polypropylene columns100 mg 200 mg 500 mg

1 ml 730061 1003 ml 730420 730063 506 ml 730421 30

CHROMABOND® CN glass columns100 mg 500 mg

1 ml 730061 G 1003 ml 730063 G 506 ml 730421 G 30

CHROMABOND® LV-CN

200 mg

15 ml 732062 30

CHROMAFIX® CN cartridges

0.4 ml / 200 mg 0.8 ml / 400 mg 1.8 ml / 900 mg

731822 731823 731824 50

CHROMABOND® MULTI 96 CN

96 x 25 mg 96 x 50 mg 96 x 100 mg

738061.025M 738061.050M 738061.100M 1

CHROMABOND® CN adsorbent

730607 100 g



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205

OH diol silica

base material silica, pore size 60 Å, particle size 45 µm,specific surface 500 m2/g, pH stability 2 – 8diol phase, carbon content 5.5 %

– polar – properties similar to SiOH

recommended application: antibioticsprostaglandins



CHROMABOND® OH polypropylene columns100 mg 200 mg 500 mg

1 ml 730051 1003 ml 730417 730053 506 ml 730418 30

CHROMABOND® OH glass columns100 mg 500 mg

3 ml 730053 G 50

CHROMABOND® LV-OH

100 mg 500 mg

15 ml 732051 732053 30

CHROMAFIX® OH cartridges

0.4 ml / 200 mg 0.8 ml / 400 mg 1.8 ml / 900 mg

731819 731820 731821 50

CHROMABOND® MULTI 96 OH

96 x 25 mg 96 x 50 mg 96 x 100 mg

738051.025M 738051.050M 738051.100M 1

CHROMABOND® OH adsorbent

730605 100 g



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206

SiOH unmodified silica

unmodified, weakly acidic silica, pore size 60 Å, particlesize 45 µm, specific surface 500 m2/g, pH stability 2 – 8

– very polar – adsorbs humidity from air, for this reason it should be

kept well closed and if necessary dried before use– due to its high affinity for polar compounds it should not

be conditioned with polar (e. g. methanol) or water-con-taining solvents

recommended application: aflatoxinschloramphenicolpesticidessteroidsvitamins



CHROMABOND® SiOH polypropylene columns100 mg 200 mg 500 mg 1000 mg 2000 mg 5 g 10 g 50 g

1 ml 730071 1003 ml 730214 730073 506 ml 730070 730075 730107 30

15 ml 730217 2045 ml 730406 2070 ml 730072 10

150 ml 730473 10

CHROMABOND® SiOH glass columns100 mg 200 mg 500 mg 1000 mg 2000 mg

1 ml 730071 G 1003 ml 730214 G 730073 G 506 ml 730070 G 730075 G 730107 G 30

CHROMABOND® LV-SiOH

100 mg 200 mg 500 mg

15 ml 732071 732072 732073 30

CHROMAFIX® SiOH cartridges

0.4 ml / 200 mg 0.8 ml / 400 mg 1.8 ml / 900 mg

731828 731829 731830 50

CHROMABOND® MULTI 96 SiOH

96 x 25 mg 96 x 50 mg 96 x 100 mg

738071.025M 738071.050M 738071.100M 1

CHROMABOND® SiOH adsorbent

730608 100 g



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207

PCA propylcarboxylic acid cation exchanger based on silica

base material silica, pore size 60 Å, particle size 45 µm,specific surface 500 m2/g, pH stability 2 – 8propylcarboxylic acid modified silica

– weakly acidic cation exchanger

recommended application: strong cations

Application: enrichment of heterocyclic compounds with cation exchangers

Compounds investigated: Desisopropylatrazine, desethyl-atrazine, atrazine, prometryn, chlorpromazine

Column types: CHROMABOND® SA, 6 ml, 500 mg, Cat. No. 730425CHROMABOND® PSA, 6 ml, 500 mg, Cat. No. 730463CHROMABOND® PCA, 6 ml, 500 mg, Cat. No. 730483CHROMAFIX® 400-PS-H+, 0.8 ml, 400 mg, Cat. No. 731861

Sample pretreatment: 200 µg desisopropylatrazine, 100 µgdesethylatrazine, 100 µg atrazine, 100 µg prometryn, and200 µg chlorpromazine are dissolved in 10 ml methanol,the solution is given in 200 ml dist. water.Column conditioning: 2 column volumes methanol, 2 col-umn volumes acetic acid (1%)Sample application: Aspirate the sample with about 700mbar through the column.Elution: 10 ml acetonitrile / 0.1 M K2HPO4 (1:1)

Subsequent analysis: HPLCColumn: CC 250/4 NUCLEOSIL® 100-5 C18

Cat. No. 721 622.40Eluent: A) water, B) methanolGradient: 3 min 40 % B, in 1 min to 60 % B, in 3 min to

90 % B, in 4 min to 100 % BFlow rate: 1 ml/minDetection: UV, 280 nmRecovery rates:


CHROMABOND® CHROMAFIX® PS-H+SA PSA PCA

Desisopropyl-atrazine

51 % 54 % 3 % 49 %

Desethylatrazine 64 % 67 % 3 % 54 %Atrazine 94 % 98 % 8 % 62 %Prometryn 96 % 97 % 10 % 41 %Chlorpromazine 71 % 99 % 15 % 35 %


CHROMABOND® PCA polypropylene columns500 mg 1000 mg

3 ml 730482 506 ml 730483 730484 30

CHROMABOND® PCA glass columns500 mg

3 ml 730482 G 50

CHROMABOND® LV-PCA

100 mg 200 mg 500 mg

15 ml 732480 732481 732482 30

CHROMABOND® MULTI 96 PCA

96 x 25 mg 96 x 50 mg 96 x 100 mg

738480.025M 738480.050M 738480.100M 1

CHROMABOND® PCA adsorbent

730629 100 g


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208

SA benzenesulphonic acid cation exchanger based on silica

base material silica, pore size 60 Å, particle size 45 µm,specific surface 500 m2/g, pH stability 2 – 8benzenesulphonic acid modified silica

– strongly acidic cation exchangercapacity ~ 0.9 meq/g

– adsorbent with hydrophobic and π-π interactions (ben-zene ring)

– ion exchange of organic compounds from aqueous ma-trix

– elution of interesting compounds with solvent systems,which compensate the ionic and nonpolar interactions,e. g. methanolic HCl

recommended application: amino acidschlorophyllPCBs



CHROMABOND® SA polypropylene columns100 mg 200 mg 500 mg 1000 mg

1 ml 730076 1003 ml 730275 730077 506 ml 730212 30

CHROMABOND® SA glass columns100 mg 200 mg 500 mg

1 ml 730076 G 1003 ml 730275 G 730077 G 50

CHROMABOND® LV-SA

100 mg 500 mg

15 ml 732081 732083 30

CHROMAFIX® SA cartridges

0.4 ml / 200 mg 0.8 ml / 400 mg 1.8 ml / 900 mg

731831 731832 731833 50

CHROMABOND® MULTI 96 SA

96 x 25 mg 96 x 50 mg 96 x 100 mg

738141.025M 738141.050M 738141.100M 1

CHROMABOND® SA adsorbent

730609 100 g



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209

PSA propylsulphonic acid cation exchanger based on silica

base material silica, pore size 60 Å, particle size 45 µm,specific surface 500 m2/g, pH stability 2 – 8propylsulphonic acid modified silica

– very strong cation exchanger – contrary to the SA phase no π-π interactions

recommended application: weak cations

Please note application 302130 ”Enrichment of heterocycliccompounds with cation exchangers“ on page 207.



CHROMABOND® PSA polypropylene columns100 mg 500 mg 1000 mg

1 ml 730460 1003 ml 730462 506 ml 730464 30

CHROMABOND® PSA glass columns100 mg 500 mg 1000 mg

1 ml 730460 G 1003 ml 730462 G 506 ml 730464 G 30

CHROMABOND® LV-PSA

100 mg 200 mg 500 mg

15 ml 732460 732461 732462 30

CHROMAFIX® PSA cartridges

0.4 ml / 200 mg 0.8 ml / 400 mg 1.8 ml / 900 mg

731710 731711 731712 50

CHROMABOND® MULTI 96 PSA

96 x 25 mg 96 x 50 mg 96 x 100 mg

738460.025M 738460.050M 738460.100M 1

CHROMABOND® PSA adsorbent

730630 100 g



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210

SB quaternary ammonium anion exchanger based on silica

base material silica, pore size 60 Å, particle size 45 µm,specific surface 500 m2/g, pH stability 2 – 8silica modified with quaternary amine

– strongly basic anion exchangercapacity ~ 0.6 meq/g

– not suited for very strong anions such as sulphonic ac-ids, because these are difficult to elute

recommended application: organic acidscaffeinesaccharin



CHROMABOND® SB polypropylene columns100 mg 200 mg 500 mg 1000 mg

1 ml 730078 1003 ml 730322 730079 506 ml 730426 730323 30

CHROMABOND® SB glass columns100 mg 200 mg 500 mg 1000 mg

1 ml 730078 G 1003 ml 730322 G 730079 G 506 ml 730426 G 30

CHROMABOND® LV-SB

100 mg 200 mg 500 mg

15 ml 732086 732087 732088 30

CHROMAFIX® SB cartridges

0.4 ml / 200 mg 0.8 ml / 400 mg 1.8 ml / 900 mg

731834 731835 731836 50

CHROMABOND® MULTI 96 SB

96 x 25 mg 96 x 50 mg 96 x 100 mg

738101.025M 738101.050M 738101.100M 1

CHROMABOND® SB adsorbent

730610 100 g



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211

PS-RP / PS-OH– / PS-H+ / PS-Ag+ / PS-Ba2+ phases for ion chromatography

base material: high purity polystyrene-divinylbenzenecopolymers (PS/DVB), pore size 100 Å, particle size100 µm

– very low degree of swelling, thus very well suited forchromatography

– reliable function over the whole pH range from 0 to 14different modifications for different applications fromelimination of nonpolar compounds up to the removal ofspecific polar components

recommended applications: – removal of interfering compounds

→ improves chromatographic separation, if the interfer-ing components overlap with the analyte in the chroma-togram (see below)→ improves lifetime of the chromatographic column,since interfering components can irreversibly block thecolumn packing

– enrichment of the analytes

Properties of the individual modifications:


PS-RP hydrophobic PS/DVB copolymer removal of organic interfering components from waterPS-OH– strong PS/DVB anion exchanger, OH– form removal or concentration of anions from water

increasing the pH value in acidic samplesPS-H+ strong PS/DVB cation exchanger, H+ form removal or concentration of cations from water

decreasing the pH value of basic samplesPS-Ag+ strong PS/DVB cation exchanger, Ag+ form removal of halide ions from waterPS-Ba2+ strong PS/DVB cation exchanger, Ba2+ form removal of sulphate ions from water


CHROMAFIX® PS cartridges0.4 ml / 200 mg 0.8 ml / 400 mg

PS-RP 731877 731875 50PS-OH– 731868 731860 50PS-H+ 731867 731861 50PS-Ag+ 731866 731865 50PS-Ba2+ 731871 731870 50

0 5min 0 5min 0 5min

1

22

1 2

original sample fraction 2 control Application: removal of halides from aqueous samples shown for the trace analysis of nitrate besides an excess of bromide

Original sample: 20 ppm nitrate besides 500 ppm bromideControl: 20 ppm nitrate besides 5 ppm bromideSample preparation: SPE

Column type: CHROMAFIX® 400-PS-Ag+

0.8 ml / 400 mg, Cat. No. 731865Column conditioning: 1 ml dist. waterSample application and elution: apply 4 x 1 ml sample frac-tions to the cartridge, discard 1st ml, collect 2nd, 3rd and 4thml separatelyChromatograms of fractions 3 and 4 correspond to that offraction 2.Analysis: HPLC

Column: CC 250/4 NUCLEOSIL® Anion II,Cat. No. 721451.40

Eluent: 2 mM potassium hydrogen phthalate pH 6Flow rate: 2 ml/minDetection: indirect UV, 280 nmPeaks: 1. bromide, 2. nitrate30

2750


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212

Alox A / Alox N / Alox B Aluminum oxide, acidic, neutral, basic

Aluminium oxide, high purity, pore volume 0.90 ml/g,particle size 60 – 150 µm, specific surface 150 m2/gAlox A: aluminium oxide, acidic, pH value 4 ± 0.3Alox N: aluminium oxide, neutral, pH value 7 ± 0.5Alox B: aluminium oxide, basic, pH value 9.5 ± 0.3

recommended application: together with phase SA for PCBs and pesticides


Phase Volume Adsorbent weight Pack of

CHROMABOND® Alox polypropylene columns100 mg 500 mg 1000 mg 4000 mg

Alox A 3 ml 730452 50Alox A 6 ml 730453 730017 30Alox A 45 ml 730455 20Alox N 3 ml 730446 50Alox N 6 ml 730447 730139 30Alox N 45 ml 730250 20Alox B 1 ml 730427 100Alox B 3 ml 730429 50Alox B 6 ml 730466 730020 30Alox B 45 ml 730467 20CHROMABOND® Alox glass columns

100 mg 500 mg 1000 mgAlox A 3 ml 730452 G 50Alox A 6 ml 730453 G 30Alox N 3 ml 730446 G 50Alox N 6 ml 730447 G 730139 G 30Alox B 1 ml 730427 G 100Alox B 3 ml 730429 G 50Alox B 6 ml 730466 G 730020 G 30CHROMABOND® LV-Alox

1000 mgAlox A 15 ml 732210 30Alox N 15 ml 732091 30Alox B 15 ml 732205 30

CHROMAFIX® Alox cartridges0.4 ml / 300 mg 0.8 ml / 700 mg 1.8 ml / 1700 mg

Alox A 731766 731767 50Alox N 731843 731844 731845 50Alox B 731761 731762 50CHROMABOND® MULTI 96 Alox

96 x 100 mgAlox A 738253.100M 1Alox N 738251.100M 1Alox B 738252.100M 1

CHROMABOND® Alox adsorbentsAlox A 730642 100 gAlox N 730641 100 gAlox B 730640 100 g



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213

Florisil® magnesium silicate

Matrix magnesium silicate (MgO-SiOH 15:85), high pu-rity, particle size 150 – 250 µm

– Florisil® is a product and registered trademark of FloridinCompany, USA.

recommended application: organic tin compoundsaliphatic carboxylic acidsPCBs, PAHs



CHROMABOND® Florisil® polypropylene columns100 mg 200 mg 500 mg 1000 mg

1 ml 730089 1003 ml 730457 730081 506 ml 730238 730082 30

CHROMABOND® Florisil® glass columns100 mg 200 mg 500 mg 1000 mg

3 ml 730457 G 730081 G 506 ml 730082 G 30

CHROMABOND® LV-Florisil®

100 mg 200 mg 500 mg

15 ml 732200 732201 732094 30

CHROMAFIX® Florisil® cartridges

0.4 ml / 200 mg 0.8 ml / 400 mg 1.8 ml / 900 mg

731846 731847 731848 50

CHROMABOND® MULTI 96 Florisil®

96 x 25 mg 96 x 50 mg 96 x 100 mg

738081.025M 738081.050M 738081.100M 1

CHROMABOND® Florisil® adsorbent

730622 100 g



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214

PA polyamide 6

matrix polyamide 6, unmodified, high purity, particle size40 – 80 µm

recommended application: flavonoids, PAHs



CHROMABOND® PA polypropylene columns100 mg 200 mg 500 mg 1000 mg

1 ml 730383 1003 ml 730384 730126 506 ml 730007 730127 30

CHROMABOND® PA glass columns100 mg 200 mg 500 mg 1000 mg

1 ml 730383 G 1003 ml 730384 G 730126 G 506 ml 730007 G 730127 G 30

CHROMABOND® LV-PA

100 mg 500 mg

15 ml 732009 732097 30

CHROMAFIX® PA cartridges

0.4 ml / 125 mg 0.8 ml / 250 mg 1.8 ml / 600 mg

731849 731850 731851 50

CHROMABOND® MULTI 96 PA

96 x 25 mg 96 x 50 mg 96 x 100 mg

738121.025M 738121.050M 738121.100M 1

CHROMABOND® PA adsorbent

730660 100 g



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