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SPE Catalog Table of Contents

Section 1 Introduction to Solid Phase Extraction (SPE) Use of Cartridges

Section 2 Overview of SPE Product Line Section 3 Sorbents Section 4 Polymer and Specialty Resins Section 5 Spe-ed eL/Lute

Spe-ed DRI Section 6 Spe-ed FLASH

Flangeless Cartridges Section 7 Method Development Kits Section 8 Accessories Section 9 Spe-ed Mate Vacuum Manifolds

930 Hamilton Street · Allentown, PA 18101 610-770-0900 · 610-740-5520 (fax) www.appliedseparations.com

SPE Catalog Section 1 Introduction to SPE 1-1 Introduction to SPE 1-8 Four Easy Steps to Prepare a Sample 1-9 Cartridge Selection Guide


Solid Phase Extraction

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www.appliedseparations.com 1-1

SPEIntroduction to Solid Phase Extraction

Matrix

Analyte

Interferences

Why Prepare a Sample?It is often necessary to isolate specific compounds for qualitative and quantitativeanalysis. An effective sample preparation technique should remove interference, concentrate analytes for greater sensitivity, or modify the sample to achieve analyticalcompatibility. Without a pure, dry high yield sample analytical results are often meaningless. For this reasons, an effective sample preparation step is crucial for successful analysis.

Defining the SampleThere are three integral components to a sample:

Matrix: The body of the sample from which you are extractingAnalyte(s): The component of the sample you are trying to extractInterferences: Other components of the sample which need to be separated from the

analyte in order to effectively clean-up the sample

Traditional Sample Preparation TechniquesSample preparation is still often performed by archaic, time consuming, and inefficient methods. Of these techniques Liquid/Liquid Extraction is the most common.

Liquid/Liquid (L/L) extraction requires taking a raw sample, usually dissolved in anaqueous, buffered solution, and placing it in a separatory funnel with an organic solvent such as Ethyl Ether, Petroleum Ether, Methylene Chloride etc. The funnel isshaken vigorously, often for long periods of time, in order to distribute the samplecomponents between the immiscible solvent and aqueous layers. The organic compounds of interest are distributed in the organic solvent layer while the inorganicsalts, acids, bases, and other water soluble components remain in the aqueous layer.

The layers are then allowed to equilibriate and separate. The solvent layer is drawnoff and the aqueous layer is extracted two more times. The solvent extracts are combined, washed with aqueous solution, and dried through anhydrous sodium sulfate. The solvent is then evaporated from the extract to concentrate the analytes.

As described, L/L extraction is very time consuming, tedious, and costly. There aremany steps, leaving a large margin for error and analyte loss. An immense amount of expensive organic solvents are used, which need to be handled and disposed ofcarefully.

To summarize, L/L sample preparation can pose the following problems:! Time consuming! Tedious and difficult procedure! Expensive due to use and disposal of large amounts of solvents! Use of large quantities of toxic and flammable solvents! Wet, contaminated extracts! Results tend to be irreproducible

Why Choose Solid Phase Extraction for Sample Preparation?Solid Phase Extraction (SPE) allows samples to be prepared nine times faster withless expense than traditional techniques. Solvent usage is greatly reduced and samples are concentrated. This alternative requires only one sample addition and isaccomplished in 4 easy steps.

How Does Solid Phase Extraction Work?Solid Phase extraction is based on the separation mechanisms of LiquidChromatography (LC). Separations take place by a competing three-way interactionof the sorbent, solvent and sample functional groups. Depending on the application,the interaction can be based on polarity or ion exchange mechanisms. Sorbents with a stronger attraction for the analyte than the sample solution are able to retain the analyte.


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DISCARD

Condition by aspiratinga few mililiters of solu-ble solvent or solutionthrough the column.

DISCARD

Matrix

ExtractionOptimize 3 Interactions

Analyte(s) Solvent

Extract and concentratecompounds of interestby aspirating a samplesolution at 5-8mL/min.

DISCARD

Remove coextractedinterferences by

aspirating 2mL or lessof suitable solvent or

solution.

ANALYZE

Elute extracted compounds by

aspirating 1mL or lessof suitable solvent or

solution.

StrongerWeaker

An elution solvent with a greater attraction to the analyte than the analyte-sorbentattraction is then used to elute the analyte of interest off of the sorbent bed.Compounds of interest are best eluted with the strongest solvent strength possible.The three way interactions are optimized to promote elution.

What is a Bonded Phase?The base silica (or polymer) used in solid phase extraction sorbents (or resins) consists of fine porous particles with a large surface area. This surface area permits adegree of functional interaction with the sample molecules passing through.

Silica gel is functionally modified by bonding organosilanes to the silanols to form abonded base.


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Solvent

ExtractionOptimize 3 Interactions

Analyte(s) Eluting Solvent

StrongerWeaker

SiOH+ Cl

Cl

SiOH

Silica Gel

Si

Si

Bonded Phase

Si SiRO

O

OH

R

Cl

Sample molecules pass through the pores and over the outer surface area of the bond-ed silica. They will interact with the functional bonded group and not the silanols.

The organo-bonded phases (R groups) can be polar, non-polar, or ionic functionally.A variety of separation mechanisms are available with varying degrees of polarity orionic strengths in order to diversify the types of applications for which solid phaseextraction can be used.


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Si

SiSi

O

O

OH

(CH2)17CH3

Octadecyl (C18)

Residual Silanol

Si

SiSi

O

O

OH SI (CH3)3(CH2)17CH3

Endcapped withTrimethylchlorosilane

What is endcapping?After bonding an organo-phase to the silica substrate, residual silanols remain due to steric exclusion. Since the free silanols are an adsorptive interaction they may compete with a different mechanism of the bonded phase. Endcapping the residualsilanols with a similar but lower molecular weight organo-phase ensures uniformmechanism interactions. In some specific applications residual silonal interactionsmay be desired. There are some non-endcapped sorbents available for use in this typeof application.

What are polymeric resins?Although silica based sorbents are most common, there are also polymer-based resinsavailable for use in solid phase extraction.

Polymeric resins work on the same principles as silica sorbents, however a polymertakes the place of the base silica. Similar to silica-based sorbents polymer-basedresins can be functionalized by either non polar, polar, or ionic functional groups.

There are some advantages to polymeric resins including:! Resins can be subjected to the full range of the pH scale (1-14), where as

sorbents break down in extreme pH conditions.! There are no residual silanol interactions because polymer based resins are

not adsorptive as are silica gels.


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PolarityTwo of the four retentionmechanisms, normal phase,and reverse phase, arerelated to the physicalproperty of polarity.Polarity is often defined asthe separation of electronic charge of a molecule or ion. The electronic charge can belocailized in the functionalgroup of a molecule offering a polar site forinteration.

The Four Retention Mechanisms

PolarityTwo of the four retention mechanisms, normal phase and reverse phase, are related to the physical property of polarity. Polarity is often defined as the separation of electronic charge of a molecule or ion. The electronic charge can be localized in thefunctional group of a molecule offering a polar site for interaction.

Normal PhaseNormal phase solid phase extraction is used to extract polar compounds from lesspolar or weaker solutions. Polar compounds are adsorbed onto the sorbent surface viapolar functional group interactions. More polar or greater solubilizing solvent thanthe sorbent is used to elute the analyte of interest form the sorbent.

The advantages of normal phases are their compatibility with aqueous solutions andtheir ability to reversibly retain polar compounds.

Reverse PhaseReverse phase SPE is used to extract non-polar or hydrophobic compounds frommore polar or weaker solutions. Non polar compounds are adsorbed onto the surfacevia non polar functional group interactions. A more non-polar or greater solubilizingsolvent than the sorbent is used for elution.

The advantages of reverse phases are their excellent compatibility with aqueous samples.

Ion exchangeIon exchange mechanisms are used to extract ionic compounds from aqueous andnon-aqueous solutions. Ionic compounds are exchanged on the sorbent surface viaopposite charges attracting eachother. A solution that will neutralize the charge interaction and has a stronger affinity to the ionic analyte is used for elution.


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SPEIntroduction to Solid Phase Extraction The following factors effect extraction and elution:

Ionic strength! counter ion concentration less than the analyte favors extraction! Counterion concentration greater than the analyte favors elution

pH

For acidic compounds:! extract 1-2 pH units above the analytes pKa! elute at an acidic pH

For basic compounds:! extract 1-2 pKa units below the analytes pKa! elute at a neutral to basic pH

Organic solvent! for ionic compounds that lose solubility in aqueous buffered eluates,

add 40-80% water miscible organic solvent

Use a weak ion exchanger if the analyte has a strong ionization. For exampleuse a weak cation exchanger if your analyte is a strong cation. Using a strongcation exchanger in this scenario may cause the analyte to be irreversiblybound to the sorbent if a counter-ion is not available to displace the analyteform the sorbent bed.

Size Exclusion MechanismsTo separate large molecules from a sample, size exclusion mechanisms areoften used. Specific resins designed for sized exclusion implement a limitingpores size to allow small molecules larger than the pores are excluded and passthrough the resin bed. This method is often used in applications such as protein and DNA purification.

Use of Spe-ed Cartridges

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Spe-ed CartridgesSpe-ed Cartridges makesample preparation easier, faster, more economical, and environmentally friendly.

Solid Phase ExtractionSolid Phase Extraction (SPE) utilizes the same analyte/sorbent interactions that are exploited in the separation technique of high performance liquid chromatography (HPLC).Spe-ed SPE cartridges are packed with a variety ofsurface-modified bonded silica sorbents which selectivly retain specific classesof chemical comounds from within a given matrix. The more specific theinteraction between the sorbent and analyte, the cleaner the final extract.

EasySpe-ed Cartridges are simple to use and allow sample preparation in four simple steps.

FastPrepare samples up to nine times faster with Spe-ed Cartridges than with traditional extraction techniques.

Economical and Environmentally FriendlyReduce the use of hazardous solvents by 95% and reduce operator time, glassware, and equipment by 83%.

ReliableSpe-ed Cartridges ensure reprodicuble results, less variablity and a smoothtransition to automated procedures. Applied Separations offers the tightestquaility control for particle size, surface activity, pH, flow rate, and interfer-ences. Packaging in hermetically sealed packets prevents contamination, andallow inspection of cartridges before use.

VersatileSpe-ed Cartridges are the most versatile, enabling sample preparation in applications such as:! pharmaceutical! clinical! environmental! toxicological! biological! foods and cosmetics! agricultural


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1 2 3 4

ConditionAdd SampleWashElute

ConditionCondition by aspirating a few millileters of suitable solvent or solution throughthe column.

Add SampleExtract and concentrate compounds of interest by aspirating a sample solution.

WashRemove co-extracted interferences by aspirating 2mL or less of suitable solventor solution.

EluteElute extracted compounds by aspirating 1mL or less of suitable solvent orsolution.

Four Easy Steps

Condition

Discard

Add Sample

Discard

Wash

Discard

Elute

Analyze

ANALYTE

INTERFERANCE

1234


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Select the Sorbent toBest Suit Your Needs

Cartridge SelectionThree Basic Extraction MechanismsThere are three basic extraction mechanisms used in solid phase extraction:non-polar, polar, and ion-exchange. Sorbent mechanism selection is primarilybased upon the funcional groups present on the analyte and the composition ofthe sample matrix.

Each sorbent within a given group exhibits unique properties of retention andselectivity which may be very specific for a given anaylte. As a result, even ifan extraction calls for a non-polar extraction mechanism, it may still be neces-sary to test several sorbents to find the optimal balance between high recoveryand good clean-up.

Sample CharacterizationThoroughly characterizing your sample is critical in selecting the right Spe-edCartridge.

Defining the SampleA sample consists of:! Martix - What you’re extracting from! Analyte - What you’re extracting! Interferences - What you don’t want extracted with the analyte

Solvent/Solution CompatibilitySpe-ed is chemically compatible with most of the common solvents and solutions used in Gas and Liquid Chromatography. Avoid sustained pH conditions below pH2 and above pH10. Do not use DMSO, sulfuric acid,hydrofluoric acid, or sodium hydroxide with Spe-ed cartridges.


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Select the Sorbent toBest Suit Your Needs Analytical Compatibility

To eliminate elute reconstitution, elute analytes with solvents/solutions compatible with the final means of analysis.

Cartridge Selection

0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 +

Hexa

neIs

ooct

ane

Carb

on T

etra

chlo

ride

Chlo

rofo

rmM

ethy

lene

Chl

orid

eTe

trahy

drof

uran

Ethy

l Eth

er, E

thyl

Acet

ate

Acet

one

Diox

ane

Acet

onitr

ile

Isop

ropa

nol

Met

hano

lW

ater

, Ace

tic A

cid

NONPOLAR

POLAR

! "

Relative Solvent Strength on Silica Gel

Solvent Miscibility

Acetic AcidWaterMethanolIsopropanolAcetonitrileAcetoneEthyl AcetateEthyl EtherMethylene ChlorideChloroformCarbon TetrachlorideHexane

Hexa

neCa

rbon

Tetra

chlor

ine

Chlor

ofor

mM

ethy

lene

Chlor

ide

Ethy

l Eth

erEt

hyl A

ceta

teAc

eton

eAc

eton

iteIso

prop

anol

Met

hano

lW

ater

Acet

ic Ac

id

# = Immiscible

##

# # # # # #

# # ######


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Select the Sorbent toBest Suit Your Needs

Cartridge SelectionCompound

Type

Nonpolar,Hydrophobic

ModeratelyNonpolarHydrophobic

Polar,Strong Cation

ModeratelyPolar

Weak Anion

Strong Anion

Basic,Weak Cation

BasicStrong Cation

Sample Solution

Polar,Aqueous

Polar,Aqueous

Less Polar,Aqueous

Less Polar,Nonaqueous

Aqueous,Nonaqueous

Aqueous,Nonaqueous

Aqueous,Nonaqueous

Aqueous,Nonaqueous

SampleVolume

<5mL5-25mL5-50mL5-500mL5mL-1L+

1-25mL5-50mL5-500mL

<5mL5-250mL

1-25mL5-250mL1-25mL5-250mL1-25mL5-250mL

1-250mL

1-250mL

1-250mL

1-250mL

Packing

Octadecyl(C18)

Octyl(C8)

Cyano(CN)

Silica Gel

Florisil®

Alumina(neutral)

Amino (NH2)

QuaternaryAmino (N+)

CarboxylicAcid (COOH)

Sulfonic Acid(SO3H)

Weight

100mg200mg500mg500mg1000mg

200mg500mg500mg

100mg500mg

500mg1000mg500mg1000mg500mg1000mg

500mg

500mg

500mg

500mg

Size

1mL3ml3mL6mL6mL

3mL3mL6mL

1mL3mL

3mL6mL3mL6mL3mL6mL

3mL

3mL

3mL

3mL

Extractables(<10,000MW)

Capacity

12mg25mg60mg60mg120mg

15mg40mg40mg

5mg25mg

40mg80mg35mg70mg35mg70mg

0.45meq

0.45meq

0.45meq

0.45meq

CatalogNo.

20012002200320062007

201220132014

22012203

210321072113211721232127

2213

2303

2313

2323

Sample Characterization Recommended Cartridges

SPE Catalog Section 2 Overview of Product Line 2-1 Spe-ed SPE Cartridges 2-2 Standard Cartridges 2-3 Super Cartridges 2-4 Spe-ed LRC Cartridges 2-5 Flangeless Cartridges 2-7 Spe-ed Flash 2-9 Mini Spe-ed Cartridges 2-11 Spe-ed 96 2-13 Spe-ed Flow 2-15 Combinatorial Chemistry Products 2-16 Method Development Kits 2-21 Accessories 2-21 Spe-ed Matrix 2-22 Bulk Sorbents


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SPE Products

Standard CartridgesStandard extraction columns arestraight walled polypropylenesyringe barrel tubes designed forfast, easy, and exact samplepreparations.

These cartridges are available in1mL, 3mL, 6mL, and 12mL sizes.

Super CartridgesApplied Separations also offerspolypropylene cartridges in largersizes, including 20mL, 35mL, and60mL.

Applied Separations offers a wide variety of cartridges types and configurations. You can be assured of reproducible results, less variability, and smooth transition to automated procedures through Applied Separations’ tight quality control for particle size,surface activity, pH, flow rate and interferances.

Spe-ed Cartridges

Spe-ed LRCThe wide opening of both the100mg and 500mg polypopylenelarge reservoir cartridge allowsfor sample or rinse volumesgreater than that allowed by thestandard cartridges. The flaredshape accommodates up to 15mL of sample without usingadditional reservoirs or adaptors.

Standard

Super

Large ReservoirCartridges(15mL)

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SPE Products

Sorbent Catalog NumberStandard Spe-ed CartridgesC18 Octadecyl C18/14%

C18 Octadecyl C18/18%


C18 Octadecyl C18/OH

2001

C8 Octyl

PH Phenyl

CH Cyclohexyl

C4 Butyl

C2 Ethyl

C1 Methyl

CNe Cyanopropyl (endcaped)

CN Cyanopropyl

SI Silica Gel

FLO Florisil

ALN Alumina (Neutral)

ALA Alumina (Acidic)

ALB Alumina (Basic)

DIO Diol

COOH Carboxylic Acid

SCX Benzenesulfonic Acid

NH2 Aminopropyl

PSA Pri/Sec Amino

DEA Diethylamino

N+ Quaternary Amino

PBA Phenylboronic Acid

100mg1mL

Standard CartridgesCartridgeSelection

For cartridges listed bysorbent, see section 3.

Sorbent packed in standard 1mL, 3mL,6mL, or 12mLpolypropylene cartridgesyringe barrels.

2002

200mg3mL

2003

500mg3mL

2006

500mg6mL

2007

1000mg6mL

2009

12001 12002 12003 12006 12007 12009

12101 12102 12103 12106 12107 12109

12201 12202 12203 12206 12207 12209

2011 2012 2013 2016 2017 2019

2081 2082 2083 2086 2087 2089

2071 2072 2073 2076 2077 2079

2021 2022 2023 2026 2027 2029

2061 2062 2063 2066 2067 2069

2051 2052 2053 2056 2057 2057

2221 2222 2223 2226 2227 2229

2201 2202 2203 2206 2207 2209

2101 2102 2103 2106 2107 2109

2111 2112 2113 2116 2117 2119

2121 2122 2123 2126 2127 2129

2131 2132 2133 2136 2137 2139

2141 2142 2143 2146 2147 2149

2151 2152 2153 2156 2157 2159

2311 2312 2313 2316 2317 2319

2321 2322 2323 2326 2327 2329

2211 2212 2213 2216 2217 2219

2241 2242 2243 2246 2247 2249

2331 2332 2333 2336 2337 2339

2301 2302 2303 2306 2307 2309

2341 2342 2343 2346 2347 2349

Qty/Box 100 50 50 30 30 20

1000mg12mL

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SPE Products

CartridgeSelection

For cartridges listedby sorbent, see section 3.

Sorbent packed insuper 6mL, 12mL,35mL, or 60mLpolypropylene cartridge syringebarrels.

Sorbent Catalog NumberSuper Spe-ed CartridgesC18 Octadecyl C18/14%




2008

C8 Octyl

PH Phenyl

CH Cyclohexyl

C4 Butyl

C2 Ethyl

C1 Methyl


CN Cyanopropyl

SI Silica Gel

FLO Florisil



ALB Alumina (Basic)

DIO Diol



NH2 Aminopropyl

PSA Pri/Sec Amino

DEA Diethylamino

N+ Quaternary Amino


2g6mL

Super Cartridges

5001

2g12mL

5002

2g35mL

5003

3g12mL

5004

3g35mL

5005

12008 15001 15002 15003 15004 15005

12108 15101 15102 15103 15104 15105

12208 15201 15202 15203 15204 15205

2018 5011 5012 5013 5014 5015

2088 5081 5082 5083 5084 5085

2078 5071 5072 5073 5074 5075

2028 5021 5022 5023 5024 5025

2068 5061 5062 5063 5064 5065

2058 5051 5052 5053 5054 5055

2228 5221 5222 5223 5224 5225

2208 5201 5202 5203 5204 5205

2108 5101 5102 5103 5104 5105

2118 5111 5112 5113 5114 5115

2128 5121 5122 5123 5124 5125

2138 5131 5132 5133 5134 5135

2148 5141 5142 5143 5144 5145

2158 5151 5152 5153 5154 5155

2318 5311 5312 5313 5314 5315

2328 5321 5322 5323 5324 5325

2218 5211 5212 5213 5214 5215

2248 5241 5242 5243 5244 5245

2338 5331 5332 5333 5334 5335

2308 5301 5302 5303 5304 5305

2348 5321 5342 5343 5344 5345

5007

15007

15107

15207

5017

5087

5077

5027

5067

5057

5227

5207

5107

5117

5127

5137

5147

5157

5317

5327

5217

5247

5337

5307

5347

5008

15008

15108

15208

5018

5088

5078

5028

5068

5058

5228

5208

5108

5118

5128

5138

5148

5158

5318

5328

5218

5248

5338

5308

5348

Qty/Box 30 20 20 20 20 20 12 12

5g35mL

10g60mL

20mg60mL

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SPE Products

Spe-ed LRCCartridgeSelection


Sorbent packed in 15mL reservoir cartridge.

Sorbent Qty/Box Catalog NumberSpe-ed LRCC18 Octadecyl C18/14%




30 3003 3005

C8 Octyl

PH Phenyl

CH Cyclohexyl

C4 Butyl

C2 Ethyl

C1 Methyl


CN Cyanopropyl

SI Silica Gel

FLO Florisil



ALB Alumina (Basic)

DIO Diol



NH2 Aminopropyl

PSA Pri/Sec Amino

DEA Diethylamino

N+ Quaternary Amino


300mg3001

30 3043 30453041

30 3093 30953091

30 3193 31953191

30 3013 30153011

30 3083 30853081

30 3073 30753071

30 3023 30253021

30 3063 30653061

30 3053 30553051

30 3223 32253221

30 3203 32053201

30 3103 31053101

30 3113 31153111

30 3123 31253121

30 3133 31353131

30 3143 31453141

30 3153 31553151

30 3313 33153311

30 3323 33253321

30 3213 32153211

30 3243 32453241

30 3333 33353331

30 3303 33053301

30 3343 33453341

100mg 500mg

SPE Products

Spe-ed Flangeless CartridgesToday’s fully automated SPE processing systems require an SPE cartridge configuration that has beenspecially adapted to fit these instru-ments. Applied Separations’ Flangelesscartridges do not have the flange, ortab that is found at the top of AppliedSeparations’ Standard SPE cartridge.

They are identical in every other way.

Applied Separations provides the same widevariety and high quality packings in these specially modified cartridges as available in its standard SPE cartridges.

The flangeless design allows for closer packing in specialized automated SPE systems, such as the Gilson® SPE215. This format will accommodate 96 x 1mL cartridges, or 48 x 3mL cartridges in the standard 96-well footprint.

If non-standard sorbents, packingamounts, or cartridge sizes arerequired, Applied Separations canprovide them.

Empty cartridges with a wide varietyof different frit material are alsoavailable.

Flangeless Cartridges

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Flangeless Standard

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SPE Products

Flangeless CartridgesSorbent Catalog NumberFlangeless CartridgesC18 Octadecyl C18/14%




C8 Octyl

PH Phenyl

CH Cyclohexyl

C4 Butyl

C2 Ethyl

C1 Methyl


CN Cyanopropyl

SI Silica Gel

FLO Florisil



ALB Alumina (Basic)

DIO Diol



NH2 Aminopropyl

PSA Pri/Sec Amino

DEA Diethylamino

N+ Quaternary Amino

100mg1mL

13100

200mg3mL

13101

500mg3mL

13102

500mg6mL

13103

1000mg6mL

13104

13106 13107 13108 13109 13110

13112 13113 13114 13115 13116

13118 13119 13120 13121 13122

13124 13125 13126 13127 13128

13130 13131 13132 13133 13134

13136 13137 13138 13139 13140

13142 13143 13144 13145 13146

13148 13149 13150 13151 13152

13154 13155 13156 13157 13158

13160 13161 13162 13163 13164

13166 13167 13168 13169 13170

13172 13173 13174 13175 13176

13178 13179 13180 13181 13182

13184 13185 13186 13187 13188

13190 13191 13192 13193 13194

13196 13197 13198 13199 13200

13202 13203 13204 13205 13206

13208 13209 13210 13211 13212

13214 13215 13216 13217 13218

13220 13221 13223 13224 13225

13226 13227 13228 13229 13230

13232 13233 13234 13235 13236

13238 13239 13240 13241 13242

13281

13282

13283

13284

13285

13286

13287

13288

13289

13290

13291

13292

13293

13294

13295

13296

13297

13298

13299

13280

13300

13301

13302

13303

13105

13111

13117

13123

13129

13135

13141

13147

13153

13159

13165

13171

13177

13183

13189

13195

13201

13207

13213

13219

13225

13231

13237

13243

Qty/Box 100 50 50 30 30 30 20

2g6mL

1000mg12mL

SPE Products

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Disposable Columns forFlash Chromatography

Spe-ed™ Flash columns have beendesigned and manufactured to meetthe critical needs of researchers whoare using automated organic purification instruments.

Buy your Flash columns from a company that actually

manufactures the packings

Get consistent results because there’s• uniformity from batch to batch

Uniform and fast flow for excellent separations, because there’s...• narrow particle size distribution

No samples are lost due to pressure problems, because there’s...• 100% inspection. Every cartridges is tested

for leaks at 100 psi

Wide range of column configurations to fit most popular instruments - even your custom configuration

Fast Delivery

Economical Pricing

Large Selection of Packings

Popular silica or SCX columnsOver 50 different packings are also available, including:

• C18 • Phenyl • C1 • Alumina • NH2 • Quat Amino • C8 • Cyclohexyl •• Cyanopropyl • Diol • PSA • C4 • C2 • Florisil • COOH • DEA • XADs •

• Polymeric SDVB, etc

SPE Products

CartridgeSelection

Representativelist only. For fulllist of cartridgesby sorbent, seesection 6.

Spe-ed Flash

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Sorbent Catalog NumberSuper Flash CartridgesSI Silica Gel


C4 Octyl

SCX

12347

SAX

HP20SS

Alumina (neutral)

D12S

12348

D12M

12301

D40S

12303

D40M

12305

D40L

12307

12353 12354 12309 12311 12313 12315

12357 12358 12317 12319 12321 12323

12361 12362 12260 12262 12264 12266

12364 12365 12268 12270 12272 12274

12367 12368 12276 12278 12280 12282

12394 12395

12425

12428

12431

12434

12437

12440

12443

12426

12429

12432

12435

12438

12441

12444

D40XL D65M D65L

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products

MiniSpe-ed CartridgesMiniSpe-ed cartridges contain the widevariety of sorbents found in standard Spe-ed cartridges. The MiniSpe-edconfiguration can be easily used in manualsyringe operations or on a vacuum mani-fold. MiniSpe-ed cartridges are also fullycompatible with instruments such asMilliab™. MiniSpe-ed cartridges have a1.0mL volume. For silica gel, this is 450mg.

MiniSpe-ed Plus CartridgesMiniSpe-ed Plus cartridges have the sameconfiguration as MiniSpe-eds, but holdalmost three times as much sorbent. TheMiniSpe-ed Plus cartridge has a volume of 2.8mL. The weight of different sorbents will varydepending on their bulk density; for silica gel this is 1265mg.

ConvenienceMiniSpe-ed / Plus cartridges stack together, interchangeably, allowing the use of more thanone sorbent, or further increasing capacity. Both cartridge types are available empty or pre-fritted for your own packing. The MiniSpe-ed and MiniSpe-ed Plus cartridges use standard20µ hydrophobic polyethylene SPE frits. Other frit material is available.

for Solid PhaseExtraction

MiniSpe-ed/MiniPlus

Syringe MiniSpe-ed or MiniSpe-ed Plus Outlet Needle

2-10TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products

Sorbent Qty/Box Catalog NumberStandard Spe-ed CartridgesC18 Octadecyl C18/14%




50 14001 24001

14002 24002

14003 24003

14004 24004

14005 24005

14006 24006

14007 24007

14009 24009

14010 24010

14011 24011

14012 24012

14013 24013

14014 24014

14015 24015

14016 24016

14017 24017

14018 24018

14019 24019

14020 24020

14021 24021

14022 24022

14023 24023

14024 24024

14025 24025

50

50

50

50

50

50

50

50

50

50

50

50

50

50

50

50

50

50

50

50

50

50

50

C8 Octyl

PH Phenyl

CH Cyclohexyl

C4 Butyl

C2 Ethyl

C1 Methyl


CN Cyanopropyl

SI Silica Gel

FLO Florisil



ALB Alumina (Basic)

DIO Diol



NH2 Aminopropyl

PSA Pri/Sec Amino

DEA Diethylamino

N+ Quaternary Amino

Mini MiniPlus

MiniSpe-ed/MiniPlusCartridgeSelection


All other packings thatApplied Separationsoffers can be ordered inthe MiniSpe-ed andMiniSpe-ed Plus format.

All cartridges are madewith 20µ hydrophobicpolyethylene frits. Othermaterials are available.Please inquire.

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products

Spe-ed 96Make SPE sample preparation easier, faster, andmore economical with Spe-ed Microtiter Plates.

EconomicalPrepare 96 samples at one time. Reduce use ofhazardous solvents, operator time, glassware,and equipment.

ReproducibleAchieve high recoveries with 2-3% coefficientsof variation.

VersatilePerform extractions with a wide variety of sorbents. Two plate sizes and a range of standardpacking amounts are available.

Spe-ed 96Solid PhaseExtraction in a96-Well Format

1. Condition 2. Add Sample 3. Wash 4. Elute

Condition byaspirating a fewmicroliters ofsuitable solventor solutionthrough the column.

Discard

Extract and concentrate compounds of interest by aspirating a sample solution.

Remove co-extracted interferences by aspirating a few microliters of suitable solvent or solution.

Elute extractedcompounds by aspirating a few microliters of suitable solvent or solution.

Discard Discard

Analyze

Cat. # Description Qty. Price24030 1mL empty extraction plate 1

Cat. # Description Qty. Price24040 2mL empty extraction plate 1 $50.0024041 2mL extraction plate w/20µ frits 1 $70.0024042 2mL round 20µ frits 100 $30.0024043 2mL square 20µ frits 100 $30.0024044 2mL collection plates 20 $90.0024045 2mL collection plate 1 $7.00

24031 1mL extraction plate w/20µ frits 1 $65.0024032 1mL 20µ frits 100 $30.0024033 1mL collection plates 20 $80.0024034 1mL collection plate 1 $6.5024029 Universal 96-well vacuum manifold 1 $1800.00

2-12TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products

CartridgeSelection


For resins and specialtysorbents, see page 4.

Spe-ed 96Sorbent Catalog NumberSpe-ed 96C18 Octadecyl C18/14%




24050

C8 Octyl

PH Phenyl

CH Cyclohexyl

C4 Butyl

C2 Ethyl

C1 Methyl


CN Cyanopropyl

SI Silica Gel

FLO Florisil



ALB Alumina (Basic)

DIO Diol



NH2 Aminopropyl

PSA Pri/Sec Amino

DEA Diethylamino

N+ Quaternary Amino

25mg1mL

24051

50mg1mL

24052

25mg2mL

24053

50mg2mL

24054

100mg2mL

24055

24060 24061 24062 24063 24064 24065

24070 24071 24072 24073 24074 24075

24080 24081 24082 24083 24084 24085

24100 24101 24102 24103 24104 24105

24110 24111 24112 24113 24114 24115

24120 24121 24122 24123 24124 24125

24130 24131 24132 24133 24134 24135

24140 24141 24142 24143 24144 24145

24150 24151 24152 24153 24154 24155

24160 24161 24162 24163 24164 24165

24170 24171 24172 24173 24174 24175

24180 24181 24182 24183 24184 24185

24190 24191 24192 24193 24194 24195

24200 24201 24202 24203 24204 24205

24210 24211 24212 24213 24214 24215

24220 24221 24222 24223 24224 24225

24230 24231 24322 24233 24234 24235

24240 24241 24242 24243 24244 24245

24250 24251 24252 24253 24254 24255

24260 24261 24262 24263 24264 24265

24270 24271 24272 24273 24274 24275

24280 24281 24282 24283 24284 24285

24290 24291 24292 24293 24294 24295

200mg2mL

SPE Products

Spe-ed FlowSpe-ed Flow cartridges are speciallydesigned to allow for the fast, free-flowing direct addition of suspended solid laden samples into a SPE cartridge. The special frit configuration of the Spe-ed Flow cartridge acts as a pre-filter to prevent clogging.

Some ApplicationsClinical/Drugs of Abuse -

Whole Blood! Direct addition of whole blood for

drugs of abuse determinations! Eliminate or minimize analyte degradation due to traditional whole blood processing

Pharmaceutical - Tissue Homogenates! Measure drugs, hormones, proteins, and other analytes without filtering first

Biotech - Fermentation Broths! Direct addition of fermentation liquors! Improve yields, no spin down necessary! Eliminate liquid/liquid extractions with more efficient SPE

Environmental - Waste Water! Eliminate filtration! No analyte loss due to analyte adsorption to the solid particles

Spe-ed FlowEnables SolidPhase Extractionfor Samples with SuspendedSolids

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Flow cartridges can be packed in any size, with any sorbent.

StandardCompound of interestInterferenceParticulates (large molecule)

Discard

Add a sample to a regularcartridge and the fritclogs. The analyte doesnot pass through the sorbent.

Frit Clog

Spe-edFlow

Compound of interestInterferenceParticulates (large molecule)

Discard the interferences.

Add a sample to a Spe-edFlow cartridge and the fritfilters the suspended solids,allowing a path for the liquid matrix to flowthrough.

Special FritConfiguration

2-14TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products

Spe-ed FlowCartridgeSelection

Spe-ed Flow cartridgescan be packed in anysize, with any sorbent.

Items shown are merelyrepresentative. Pleaseinquire.

Size Qty/Box Catalog NumberSpe-ed Flow ABN3mL

6mL

15mL LRC

15mL LRC with cap

50 2723

30 2724

30 2725

30 3725

Spe-ed Flow THC3mL

6mL

15mL LRC

15mL LRC with cap

50 2619

30 2620

30 2621

30 2622

Spe-ed Flow THC II3mL

6mL

15mL LRC

15mL LRC with cap

50 2623

30 2624

30 2625

30 2626

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products

Combinatorial Chemistry AccessoriesMany of our products are used by combinatorialchemistry laboratories. Some places to find theseproducts in our catalog include:AccessoriesSEE SECTION 8

DESCRIPTIONFind a full line of empty cartridges and fritted cartridges to use as reaction vessels.We offer caps and tips for all of our cartridges to seal off reaction vessels and to protect reactions from contamination.Check out the chemically inert PTFE stopcocks.We also have universal adaptors to stack reaction vessels vessels.Frits of varying pore sizes are available in several materials, including PTFE.

Solid Phase Extraction Columns and 96-Well PlatesWe offer a full line of sorbents and resins. The following is a list of popular combinatorialpacking materials

SCX Benzenesulfonic AcidN+ Quaternary AminoSI Silica GelAlumina (Available in acidic, basic and neutral)Florisil®C18C8NH2 Aminopropyl

Bulk Sorbents and ResinsAll of our sorbents and resins are available in bulk.

Custom Packing ServicesWe will pack a specified mass of your sorbents or ours in any size column. Please inquire.This can be a great opportunity to save time in your laboratory.

CombinatorialChemistry Productsfor Solid PhaseExtraction

2-16TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products

Method DevelopmentKitsEach Method Development Kitcontains a sampling of sorbentsfor developing and optimizingsample preparation methodsand procedures.

Method DevelopmentKitsfor Solid PhaseExtraction

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products

Method DevelopmentKits

Qty. Description10201010

C18 Octadecyl/14C18 Octadecyl/18C18 Octadecyl/22C18 Octadecyl/OH

Non-Polar

#2501

500mg/3mL50 cartridges



Non-Polar

#2554

5g/20mL16 cartridges



Non-Polar

#2550100mg/1mL$8048 cartridges



Non-Polar

#2551




Non-Polar

#2552




Non-Polar

#2553



C8 OctylPH PhenylCH Cyclohexyl

ModerateNon-Polar#2502






Qty. Description888




Qty. Description888




Qty. Description444




Qty. Description444




Non-Polar Kits Moderate Non-Polar Kits

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SPE Products

Method Development Kits


C4 ButylC2 EthylC1 MethylCNe Cyanopropyl (endcapped)


CN CyanopropylSI SilicaFLO Florisil®

ALN Alumina (Neutral)ALA Alumina (Acidic)ALB Alumina (Basic)DIO Diol

Polar#2505





Polar#2571





Polar#2572





Polar#2573





Polar#2574





Polar#2575


WeakNon-Polar#2504




WeakNon-Polar#2564




WeakNon-Polar#2565




WeakNon-Polar#2566




WeakNon-Polar#2567




WeakNon-Polar#2568


Weak Non-Polar Kits Polar Kits

® Florisil is a registered trademark of Floridin Co.

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products



COOH Carboxylic AcidSCX Benzenesulfonic AcidNH2 AminopropylPSA Primary/Secondary AmineDEA DiethylaminoN+ Quaternary Amino

Ion-Exchange#2506




Ion-Exchange#2581




Ion-Exchange#2582

2.5g/20mL24 cartridges



Ion-Exchange#2583




Ion-Exchange#2578




Ion-Exchange#2579




Ion-Exchange#2580


Ion-Exchange Kits

!"!"Custom kits

containing varying sizes and combinations are also available.!"!"

2-20TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products



RP-101RP-102RP-103RP-104RP-105

#2585



RP-101RP-102RP-103RP-104

RPResins#2586



RP-101RP-102RP-103RP-104RP-105

RPResins#2587



RP-101RP-102RP-103RP-104

RPResins#2588



RP-101RP-102RP-103RP-104RP-105

#2589


RPResins


RP-101RP-102RP-103RP-104

RPResins#2590



RP-101RP-102RP-103RP-104RP-105

#2591


RPResins


RP-101RP-102RP-103RP-104

RPResins#2592



RP-101RP-102RP-103RP-104RP-105

#2593


RPResins


RP-101RP-102RP-103RP-104

RPResins#2594


RP Resin KitsRP101, 102, 103, 104, 105

RP Resin KitsRP101, 102, 103, 104


Amberlite® XAD-2Amberlite® ZAD-4Amberlite® XAD-7Amberlite® XAD-16

Amberlite®

#2507


Amberlite® XAD Kits

® Amberlite is a registered trademark of Rohm and Haas Co.

RPResins

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products

AccessoriesApplied Separations offers a variety of SPE accessories including medical grade polypropylene cartridges, frits, filters, syringe plungers, adaptors, and stopcocks.

The polypropylene cartridges are available in sizesranging from 1mL to 60mL. MiniSpe-ed, and largereservoir cartridges (LRC). These cartridges may bepurchased empty or with frits.

Separate frits of assorted materials are also available inorder to meet individual extraction needs. Syringeplungers are sold separately for manual extraction.

These accessories, used separately, or in combiantion,result in customized extraction columns for use in allsolid phase extraction procedures.

AccessoriesEmpty CartridgesFritsFiltersSyringe PlungersAdaptorsCaps & TipsStopcocks

Spe-ed MatrixSpe-ed Matrix is a modified form ofdiatomaceous earth that can be packedinto any fritted Spe-ed SPE cartridge foruse as a support in sample preparation.

Spe-ed Matrix is also ideal for use insupercritical fluid extraction.


Spe-ed Matrix

2-22TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


SPE Products


Bulk SorbentsSorbent Catalog NumberStandard Spe-ed CartridgesC18 Octadecyl C18/14%




4100 4132 4162

4101 4133 4163

4102 4164

4103 4135 4165

4104 4166

4105 4167

4106 4168

4107

4108 4170

4109 4171

4110

4118 4177

4111 4173

4112 4174

4113 4189

4114 4190

4115 4191

4116 4175

4119 4178

4120 4152 4179

4121 4153 4180

4123 4154 4181

4124 4155 4182

4125 4156 4183

C8 Octyl

PH Phenyl

CH Cyclohexyl

C4 Butyl

C2 Ethyl

C1 Methyl


CN Cyanopropyl

SI Silica Gel

FLO Florisil



ALB Alumina (Basic)

DIO Diol



NH2 Aminopropyl

PSA Pri/Sec Amino

DEA Diethylamino

N+ Quaternary Amino

100g 500g 1Kg

SPE Catalog Section 3 Sorbents 3-1 C18/14% 3-2 C18/18% 3-3 C18/22% 3-4 C8 (Octyl) 3-5 PH (Phenyl) 3-6 CH (Cyclohexyl) 3-7 C18/OH (Octadecyl/18/OH)

Carbon Loading / Not Endcapped 3-8 CNe (Cyanopropyl) 3-9 C4 (Butyl) 3-10 C2 (Ethyl) 3-11 C1 (Methyl) 3-12 CN (Cyanopropyl)

Not endcapped 3-13 Si (Silica Gel) 3-14 FLO (Florisil®) 3-15 ALA (Acidic Alumina) 3-16 ALN (Neutral Alumina) 3-17 ALB (Basic Alumina) 3-18 DIO (Diol) 3-19 COOH (Carboxylic Acid) 3-20 SCX (Benzenesulfonic Acid) 3-21 NH2 (Aminopropyl) 3-22 PSA (Primary/Secondary Amine) 3-23 DEA (Diethylamino) 3-24 N+ (Quaternary Amino) 3-25 PBA (Phenylboronic Acid)


Non-Polar (Reverse Phase)

Octadecyl/18Carbon Loading: 14%

Description of Base Sorbent! 40µm irregularly-shapedsilica,60 Angstrom mean porosity

The primary retention mechanism is strongly non-polar with typical sample matrices of waterand aqueous biological fluids.

Description Qty/Box Catalog NumberStandard Spe-ed Cartridges100mg/1mL 100 2001

200mg/3mL 50 2002

C18/14%

500mg/3mL 50 2003

500mg/6mL 30 2006

1000mg/6mL 30 2007

1000mg/12mL 20 2009

Super Spe-ed Cartridges2g/6mL 30 2008

2g/12mL 20 5001

2g/35mL 20 5002

LRC Cartridges100mg/15ml 30 3001

300mg/15mL 30 3003

Mini Spe-ed/Mini Spe-ed PlusMini Spe-ed 50 14001

Mini Spe-ed Plus 50 24001

500mg/15mL 30 3005

3g/12mL 20 5003

3g/35mL 20 5004

5g/35mL 20 5005

Spe-ed-9625mg/1mL plate 1 24050

50mg/1mL plate 1 24051



100mg/2mL plate 1 24054

200mg/2mL plate 1 24055

10g/60mL 12 5007

20g/60mL 12 5008

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520




Description of Base Sorbent! 40µm irregularly-shapedacid silica, 60 Angstrommean porosity



200mg/3mL 50 12002

C18/18%

500mg/3mL 50 12003

500mg/6mL 30 12006

1000mg/6mL 30 12007

1000mg/12mL 20 12009


2g/12mL 20 15001

2g/35mL 20 15002

LRC Cartridges100mg/15mL 30 3041

300mg/15mL 30 3043



500mg/15mL 30 3045

3g/12mL 20 15003

3g/35mL 20 15004

5g/35mL 20 15005





100mg/2mL plate 1 24064

200mg/2mL plate 1 24065

10g/60mL 12 15007

20g/60mL 12 15008

3-2TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520




Description of Base Sorbent! 40µm/120µm irregularly-shaped acid washed silica,60 Angstrom mean porosity

The primary retention mechanism is strongly non-polar with typical sample matrices of water andaqueous biological fluids.


200mg/3mL 50 12102

C18/22%

500mg/3mL 50 12103

500mg/6mL 30 12106

1000mg/6mL 30 12107

1000mg/12mL 20 12109


2g/12mL 20 15101

2g/35mL 20 15102


300mg/15mL 30 3093



500mg/15mL 30 3095

3g/12mL 20 15103

3g/35mL 20 15104

5g/35mL 20 15105





100mg/2mL plate 1 24074

200mg/2mL plate 1 24075

10g/60mL 12 15107

20g/60mL 12 15108

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Moderate Non-Polar (Reverse Phase)

Octyl

Description of Base Sorbent! 40µm/120µm irregularacid washed silica,60 Angstrom mean porosity

The primary retention mechanism is moderately non-polar with typical sample matrix of water andaqueous biological fluids.


200mg/3mL 50 2012

C8

500mg/3mL 50 2013

500mg/6mL 30 2016

1000mg/6mL 30 2017

1000mg/12mL 20 2019


2g/12mL 20 5011

2g/35mL 20 5012


300mg/15mL 30 3013



500mg/15mL 30 3015

3g/12mL 20 5013

3g/35mL 20 5014

5g/35mL 20 5015





100mg/2mL plate 1 24104

200mg/2mL plate 1 24105

10g/60mL 12 5017

20g/60mL 12 5018

3-4TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Moderately Non-Polar (Reverse Phase)

Phenyl


The primary retention mechanism is moderately non-polar with typical sample matrix of water andaqueous biological fluids.


200mg/3mL 50 2082

PH

500mg/3mL 50 2083

500mg/6mL 30 2086

1000mg/6mL 30 2087

1000mg/12mL 20 2089


2g/12mL 20 5081

2g/35mL 20 5082


300mg/15mL 30 3083



500mg/15mL 30 3085

3g/12mL 20 5083

3g/35mL 20 5084

5g/35mL 20 5085





100mg/2mL plate 1 24114

200mg/2mL plate 1 24115

10g/60mL 12 5087

20g/60mL 12 5088

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520



Cyclohexyl


The primary retention mechanism is moderately non-polar with typical sample matrix of water andaqueous fluids.


200mg/3mL 50 2072

CH

500mg/3mL 50 2073

500mg/6mL 30 2076

1000mg/6mL 30 2077

1000mg/12mL 20 2079


2g/12mL 20 5071

2g/35mL 20 5072


300mg/15mL 30 3073



500mg/15mL 30 3075

3g/12mL 20 5073

3g/35mL 20 5074

5g/35mL 20 5075





100mg/2mL plate 1 24124

200mg/2mL plate 1 24125

10g/60mL 12 5077

20g/60mL 12 5078

3-6TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520



Octadecyl/18/OHCarbon Loading Not endcapped


The primary retention mechanism is moderately non-polar with typical sample matrix of aqueousbiological fluids.

The lack of endcappingresults in significant polarsecondary interactions.


200mg/3mL 50 12202

C18/OH

500mg/3mL 50 12203

500mg/6mL 30 12206

1000mg/6mL 30 12207

1000mg/12mL 20 12209


2g/12mL 20 15201

2g/35mL 20 15202


300mg/15mL 30 3193



500mg/15mL 30 3195

3g/12mL 20 15203

3g/35mL 20 15204

5g/35mL 20 15205





100mg/2mL plate 1 24084

200mg/2mL plate 1 24085

10g/60mL 12 15207

20g/60mL 12 15208

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520



Cyanopropyl


The primary retention mechanism is polar withaqueous matrices and polarwith non-polar matrices.


200mg/3mL 50 2222

CNe

500mg/3mL 50 2223

500mg/6mL 30 2226

1000mg/6mL 30 2227

1000mg/12mL 20 2229


2g/12mL 20 5221

2g/35mL 20 5222


300mg/15mL 30 3223



500mg/15mL 30 3225

3g/12mL 20 5223

3g/35mL 20 5224

5g/35mL 20 5225





100mg/2mL plate 1 24164

200mg/2mL plate 1 24165

10g/60mL 12 5227

20g/60mL 12 5228

3-8TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Moderate Non-Polar (Reverse Phase)

Butyl


The primary retention mechanism is moderately non-polar with typical sample matrices of waterand aqueous biological fluids.


200mg/3mL 50 2022

C4

500mg/3mL 50 2023

500mg/6mL 30 2026

1000mg/6mL 30 2027

1000mg/12mL 20 2029


2g/12mL 20 5021

2g/35mL 20 5022

LRC Cartridges100mg/15ml 30

300mg/15mL 30 3023



500mg/15mL 30 3025

3g/12mL 20 5023

3g/35mL 20 5024

5g/35mL 20 5025





100mg/2mL plate 1 24134

200mg/2mL plate 1 24135

10g/60mL 12 5027

20g/60mL 12 5028

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Weak Non-Polar (Reverse Phase)

Ethyl


The primary retention mechanism is weakly non-polar with typical sample matrices of plasma,urine and aqueous fluids.


200mg/3mL 50 2062

C2

500mg/3mL 50 2063

500mg/6mL 30 2066

1000mg/6mL 30 2067

1000mg/12mL 20 2069


2g/12mL 20 5061

2g/35mL 20 5062


300mg/15mL 30 3063



500mg/15mL 30 3065

3g/12mL 20 5063

3g/35mL 20 5064

5g/35mL 20 5065





100mg/2mL plate 1 24144

200mg/2mL plate 1 24145

10g/60mL 12 5067

20g/60mL 12 5068

3-10TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Weak Non-Polar (Reverse Phase)

Methyl


The primary retention mechanism is very weakly non-polar with typical sample matrices of plasma,urine and aqueous fluids.


200mg/3mL 50 2052

C1

500mg/3mL 50 2053

500mg/6mL 30 2056

1000mg/6mL 30 2057

1000mg/12mL 20 2059


2g/12mL 20 5051

2g/35mL 20 5052


300mg/15mL 30 3053



500mg/15mL 30 3055

3g/12mL 20 5053

3g/35mL 20 5054

5g/35mL 20 5055





100mg/2mL plate 1 24154

200mg/2mL plate 1 24155

10g/60mL 12 5057

20g/60mL 12 5058

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Polar (Normal Phase)

CyanopropylNot endcapped


The primary retention mechanism is polar withtypical sample matrices ofnon-polar solvents andweakly non-polar withaqueous sample matrices.


200mg/3mL 50 2202

CN

500mg/3mL 50 2203

500mg/6mL 30 2206

1000mg/6mL 30 2207

1000mg/12mL 20 2209


2g/12mL 20 5201

2g/35mL 20 5202


300mg/15mL 30 3203



500mg/15mL 30 3205

3g/12mL 20 5203

3g/35mL 20 5204

5g/35mL 20 5205





100mg/2mL plate 1 24174

200mg/2mL plate 1 24175

10g/60mL 12 5207

20g/60mL 12 5208

3-12TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520



Silica Gel

Description of Base Sorbent! 40µm/120µm irregularsilica, 60 Angstrom meanporosity, unfunctionalizedsilica.

The primary retention mechanism is stronglypolar with typical sample matrices of non-polar organic solvents, oils and lipids.


200mg/3mL 50 2102

Si

500mg/3mL 50 2103

500mg/6mL 30 2106

1000mg/6mL 30 2107

1000mg/12mL 20 2109


2g/12mL 20 5101

2g/35mL 20 5102


300mg/15mL 30 3103



500mg/15mL 30 3105

3g/12mL 20 5103

3g/35mL 20 5104

5g/35mL 20 5105





100mg/2mL plate 1 24184

200mg/2mL plate 1 24185

10g/60mL 12 5107

20g/60mL 12 5108

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520



Florisil®

Description of Base Sorbent! Florisil®, 200µm particlesize.

The primary retention mechanism is polar withenvironmental and organicextracts as typical samplematrices.


200mg/3mL 50 2112

FLO

500mg/3mL 50 2113

500mg/6mL 30 2116

1000mg/6mL 30 2117

1000mg/12mL 20 2119


2g/12mL 20 5111

2g/35mL 20 5112


300mg/15mL 30 3113



500mg/15mL 30 3115

3g/12mL 20 5113

3g/35mL 20 5114

5g/35mL 20 5115





100mg/2mL plate 1 24194

200mg/2mL plate 1 24195

10g/60mL 12 5117

20g/60mL 12 5118

3-14TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Florisil® is a registered trademark of Floridin Co.


Aluminaacidic

Description of Base Sorbent! Alumina, Al2O3, acidicparticle size 500 - 200µm,pH 4.5

The primary retention mechanisms are polar,Lewis acid/base and ionexchange.

All alumina activity level1.(Level 2 - 5 also available.Please inquire.)


200mg/3mL 50 2132

ALA

500mg/3mL 50 2133

500mg/6mL 30 2136

1000mg/6mL 30 2137

1000mg/12mL 20 2139


2g/12mL 20 5131

2g/35mL 20 5132


300mg/15mL 30 3133



500mg/15mL 30 3135

3g/12mL 20 5133

3g/35mL 20 5134

5g/35mL 20 5135





100mg/2mL plate 1 24214

200mg/2mL plate 1 24215

10g/60mL 12 5137

20g/60mL 12 5138

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520



Aluminaneutral

Description of Base Sorbent! Alumina, Al2O3, neutralparticle size 50 - 20µm, pH 7.5


All alumina activity level 1. (Level 2 - 5 also available.Please inquire.)


200mg/3mL 50 2122

ALN

500mg/3mL 50 2123

500mg/6mL 30 2126

1000mg/6mL 30 2127

1000mg/12mL 20 2129


2g/12mL 20 5121

2g/35mL 20 5122


300mg/15mL 30 3123



500mg/15mL 30 3125

3g/12mL 20 5123

3g/35mL 20 5124

5g/35mL 20 5125





100mg/2mL plate 1 24204

200mg/2mL plate 1 24205

10g/60mL 12 5127

20g/60mL 12 5128

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Aluminabasic

Description of Base Sorbent! Alumina, Al2O3, basicparticle size 5- - 200µm,pH 10


All alumina activity level 1.(Level 2 - 5 also available.Please inquire.)


200mg/3mL 50 2142

ALB

500mg/3mL 50 2143

500mg/6mL 30 2146

1000mg/6mL 30 2147

1000mg/12mL 20 2149


2g/12mL 20 5141

2g/35mL 20 5142


300mg/15mL 30 3143



500mg/15mL 30 3145

3g/12mL 20 5143

3g/35mL 20 5144

5g/35mL 20 5145





100mg/2mL plate 1 24224

200mg/2mL plate 1 24225

10g/60mL 12 5147

20g/60mL 12 5148

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Diol


The primary retention mechanism is polar withtypical sample matrices ofnon-polar organic extracts,oil and lipids.


200mg/3mL 50 2152

DIO

500mg/3mL 50 2153

500mg/6mL 30 2156

1000mg/6mL 30 2157

1000mg/12mL 20 2159


2g/12mL 20 5151

2g/35mL 20 5152


300mg/15mL 30 3153



500mg/15mL 30 3155

3g/12mL 20 5153

3g/35mL 20 5154

5g/35mL 20 5155





100mg/2mL plate 1 24234

200mg/2mL plate 1 24235

10g/60mL 12 5157

20g/60mL 12 5158

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Cation Exchangers

Carboxylic Acid

Description of Base Sorbent! 40µm irregular acidwashed silica, 60 Angstrommean porosity, pKa 4.8

The primary retention mechanism is weak cationexchange with typical sample matrices of waterand biological fluids.


200mg/3mL 50 2312

COOH

500mg/3mL 50 2313

500mg/6mL 30 2316

1000mg/6mL 30 2317

1000mg/12mL 20 2319


2g/12mL 20 5311

2g/35mL 20 5312


300mg/15mL 30 3313



500mg/15mL 30 3315

3g/12mL 20 5313

3g/35mL 20 5314

5g/35mL 20 5315





100mg/2mL plate 1 24244

200mg/2mL plate 1 24245

10g/60mL 12 5317

20g/60mL 12 5318

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Cation Exchangers

Benzenesulfonic Acid

Description of Base Sorbent! 40µm/120µm irregularacid washed silica,60 Angstrom mean porositypKa: <<1.0

The primary retention mechanism is strong cationexchange with typical sample matrices of aqueous,biological fluids.


200mg/3mL 50 2322

SCX

500mg/3mL 50 2323

500mg/6mL 30 2326

1000mg/6mL 30 2327

1000mg/12mL 20 2329


2g/12mL 20 5321

2g/35mL 20 5322


300mg/15mL 30 3323



500mg/15mL 30 3325

3g/12mL 20 5323

3g/35mL 20 5324

5g/35mL 20 5325





100mg/2mL plate 1 24254

200mg/2mL plate 1 24255

10g/60mL 12 5327

20g/60mL 12 5328

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Anion Exchangers

Aminopropyl

Description of Base Sorbent! 40µm/120µm irregularacid washed silica,60 Angstrom mean porositypKa: 9.8

The primary retention mechanism is weak anionexchange with typical sample matrices of water,biological fluids, andorganic extracts.


200mg/3mL 50 2212

NH2

500mg/3mL 50 2213

500mg/6mL 30 2216

1000mg/6mL 30 2217

1000mg/12mL 20 2219


2g/12mL 20 5211

2g/35mL 20 5212


300mg/15mL 30 3213



500mg/15mL 30 3215

3g/12mL 20 5213

3g/35mL 20 5214

5g/35mL 20 5215





100mg/2mL plate 1 24264

200mg/2mL plate 1 24265

10g/60mL 12 5217

20g/60mL 12 5218

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Anion Exchangers

Primary/SecondaryAmine

Description of Base Sorbent! 40µm/120µm irregularacid washed silica,60 Angstrom mean porositypKa: 10.1 and 10.9

The primary retention mechanism is weak anionexchange with aqueoussamples, polar with non-polar organic matrices.Typical sample matricesinclude water, biological fluids and organic extracts.


200mg/3mL 50 2242

PSA

500mg/3mL 50 2243

500mg/6mL 30 2246

1000mg/6mL 30 2247

1000mg/12mL 20 2249


2g/12mL 20 5241

2g/35mL 20 5242


300mg/15mL 30 3243



500mg/15mL 30 3245

3g/12mL 20 5243

3g/35mL 20 5244

5g/35mL 20 5245





100mg/2mL plate 1 24274

200mg/2mL plate 1 24275

10g/60mL 12 5247

20g/60mL 12 5248

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Anion Exhcangers

Diethylamino

Description of Base Sorbent! 40µm/120µm irregularacid washed silica,60 Angstrom mean porositypKa: 10.7

The primary retention mechanism is weak anionexchange with samplematrices of water, biologi-cal fluids, and polar withnon-polar extracts.


200mg/3mL 50 2332

DEA

500mg/3mL 50 2333

500mg/6mL 30 2336

1000mg/6mL 30 2337

1000mg/12mL 20 2339


2g/12mL 20 5331

2g/35mL 20 5332


300mg/15mL 30 3333



500mg/15mL 30 3335

3g/12mL 20 5333

3g/35mL 20 5334

5g/35mL 20 5335





100mg/2mL plate 1 24284

200mg/2mL plate 1 24285

10g/60mL 12 5337

20g/60mL 12 5338

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Anion Exchangers

Quaternary Amino

Description of Base Sorbent! 40µm/120µm irregularacid washed silica,60 Angstrom mean porosityQuaternary amine is alwayscharged.

The primary retention mechanism is strong anionexchange with typical sample matrices of aqueousand biological fluids.


200mg/3mL 50 2302

N+

500mg/3mL 50 2303

500mg/6mL 30 2306

1000mg/6mL 30 2307

1000mg/12mL 20 2309


2g/12mL 20 5301

2g/35mL 20 5302


300mg/15mL 30 3303



500mg/15mL 30 3305

3g/12mL 20 5303

3g/35mL 20 5304

5g/35mL 20 5305





100mg/2mL plate 1 24294

200mg/2mL plate 1 24295

10g/60mL 12 5307

20g/60mL 12 5308

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Covalent

Phenylboronic Acid


The primary retention mechanism is a covalentbond with typical sample matrices of aqueousand biological fluids posessing vicinal Diolgroups.


200mg/3mL 50 2342

PBA

500mg/3mL 50 2343

500mg/6mL 30 2346

1000mg/6mL 30 2347

1000mg/12mL 20 2349


2g/12mL 20 5341

2g/35mL 20 5342


300mg/15mL 30 3343

500mg/15mL 30 3345

3g/12mL 20 5343

3g/35mL 20 5344

5g/35mL 20 5345

10g/60mL 12 5347

20g/60mL 12 5348

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SPE Catalog Section 4 Polymer and Specialty Resins 4-1 RP Resins 4-2 RP101 4-3 RP102 4-4 RP103 4-5 RP104 4-6 Spe-ed Advanta 4-8 Amberlite® XAD 4-9 XAD-4 4-10 XAD-7 4-11 XAD-16 4-12 XAD-1180 4-13 XAD-2000 4-14 XAD-2010 4-15 Spe-ed Amide-2 4-17 PuriPON Columns 4-19 For Drugs of Abuse

Spe-ed Scan Spe-ed Flow


930 Hamilton St.Allentown, PA

18101610.770.0900

610.740.5520 (fax)spe02 02/04


Spe-eed™ RP Resins

Solid PhaseExtraction

SP

E Reverse Phase Polymer-based Resins for Sample Preparation

Spe-ed RP resins are a family of polymer-ic media useful for sample preparation.Spe-ed RP resins are porous sphericalbeads, contain a high surface area and arechemically stable to maximize extraction efficiency.

Directly substitute Spe-ed RP resins in your current applications wheresilica-based C18 may not be giving you optimum recovery.

pH stable (1 - 14)process high and low pH samples without sorbent decomposition like C18retain strongly basic amines at high pH conditions

Eliminate residual silanolseliminate secondary interaction caused by residual silanols on C18/silica SPE cartridges (use exclusively reverse phase mechanism)

Biomolecules to small drugsthe range of pore sizes allows the application of Spe-ed RPcartridges for many types of molecules from small molecule drugs to oligonucleotidesoptimized particle size for easy throughput of biological samples such as urine, plasma or serum

Applicationsreplace C18/silica SPE cartridges with pH resistant Spe-ed RP resinsremove endotoxinsexchange solventsseparate hydrophobic molecules by sizedesalt samplesenvironmental - herbicide / pesticide residuessulfonylurea herbicides

Substitute for C18 SPE CartridgesSpe-ed RP resin cartridges offer many advantages.

Size excluded molecules (large bio molecules)

Bound analyte of interest

100Apore

FLOW

Active sorbent site

40,000 daltons

RP102

Particle SizeUniformity

Zero FinesLarge Surface Area

Leads toExcellent Extraction Efficiency High Recoveries

Large bio molecules are not adsorbed ontothe RP resin surface. Because of their largersize, they are excluded from adsorbing ontothe active sorbent site inside the pores.Smaller molecules are trapped on the activesorbent sites in these pores.

930 Hamilton St. • Allentown, PA 18101610.770.0900 • 610.740.5520 (fax)


spe02 02/04

Spe-eed™ RP Resins

RP Resin CharacteristicsRP resins are non-polar, non-ionic, ultra-clean, highly crossed-linked polymers that differ in particle size,pore size and surface area. Select a resin with a pore size large enough to allow your analyte to enter thepore and adsorb onto the pore surface.

Resin SelectionSpe-ed RP cartridges are pre-packed with measured amounts of dry, free-flowing, chromatographicresin. The cartridges are made of medical grade polypropylene. These pre-filled cartridges are availablein a wide range of sorbent weight and cartridge size combinations.

RP104

RP103

RP102

RP101

STANDARD SIZES

35

75

100

120

800,000

300,000

400,000

70,000

PARTICLE SIZE(AVG)

microns

EXCLUSION LIMIT

daltons

RP101100mg/1mL 200mg/3mL 500mg/6mL

4201 4202 4204

4207 4208 4210

4213 4214 4216

4219 4220 4222

100 50 30

RP102

RP103

RP104

cartridges/box

50mg/1mL

4295

4299

4296

4297

100

Custom resin weights available.

Phenolp-Cresolp-EthylphenolNitrobenzene2.4 DimethylphenolAnilineBenzyl alcohol2.4 DinitrofluorobenzeneMethythydroquinone

* Recovery data for additional analytes available upon request.** Polystryene Divinyl Benzene

930 Hamilton St.Allentown, PA

18101610.770.0900

610.740.5520 (fax)spe25 02/04


Spe-ed™ Advanta

Solid PhaseExtraction

SP

E Isolation of Polar Organics from Aqueous Matrices

Applied Separations, Inc., has developed Spe-ed Advanta, a new chemically modifiedpolymeric resin for solid phase extraction (SPE).Spe-ed Advanta is packed in standard 1, 3 and6mL SPE cartridges, and in 96-well plates (othercartridge sizes available upon request) and iseffective in removing polar organic compoundsfrom aqueous samples.

Unlike standard C18 and polystyrene divinyl-benzene SPE cartridges, Spe-ed Advanta is:• easily wetted with water• requires no tedious conditioning steps• eliminates poor recoveries associated with the drying of a

conditioned sorbent bed.

Spe-ed Advanta markedly increases the recoveries of polar compounds fromaqueous matrices when compared to C18 and polystyrene divinylbenzene SPEcartridges. Spe-ed Advanta enhances the preparation of pharmaceutical, envi-ronmental, biological, clinical, biotechnical, food and cosmetic samples.

Recoveries of phenols, aromatic compounds and polyhydroxy aromatic compounds by SPE under wet and dry loading conditions.*

AddAqueousSample

Wash Elute Polar

Analyte

1 2 3

Wet616665471910440

Dry34152721<5<540

Wet919196929594928377

Dry3123751422617230

Wet100101101100100100999899

Dry939499979896999494

Recovery Percentage

CompoundC18 DVB** Advanta

930 Hamilton St. • Allentown, PA 18101610.770.0900 • 610.740.5520 (fax)


spe25 02/04

Spe-eed™ Advanta

SSttaannddaarrdd SSppee-eedd AAddvvaannttaa

SSppee-eedd FFllooww AAddvvaannttaa**

* Spe-ed Flow cartridges are specially designed to allow for the fast, free-flowing direct addition of suspended solid laden samplesinto an SPE cartridge. Please refer to the Spe-ed Flow literature for additional information.

Larger cartridge configurations are available upon request.

30mg/1mL

Description

50mg/1mL

100mg/1mL

100mg/3mL

200mg/3mL

500mg/6mL

100

100

100

50

50

30

Qty/Box

4261

4262

4263

4264

4265

4266

Catalog Number

30mg/1mL

Description

50mg/1mL

100mg/1mL

100mg/3mL

200mg/3mL

500mg/6mL

100

100

100

50

50

30

Qty/Box

4267

4268

4269

4270

4271

4272

Catalog Number

4-8TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Amberlite® XAD Resins

PrecleanedPrepackedPreconditioned

Amberlite® XAD Spe-ed Amberlite® XAD ResinsXAD-2, XAD-4, XAD-7, and XAD-16 resins are neutral, macrop-orous polymeric beads and derive their adsorbative characteristicsfrom their macroreticular porosity, pore size distribution, high surfacearea, and chemically homogeneous non-ionic structure. These resinsare primarily used to extract non-polar, hydrophobic compounds fromaqueous or polar solutions.

Pre-cleaned and Pre-swollen to Eliminate VariabliltySpe-ed cartridges are prefilled with measured amounts of speciallyprepared and cleaned XAD resin for critical analytical use.Commercial XAD resins are normally not suitable for analytical orother applications requiring high purity. Applied Separations’ XADproducts are given mulitiple solvent washes to assure the highest levelof quality. Variablity problems are eliminated with pre-swollen, pre-conditioned beads. To ensure continuing quality, the cartridge with theXAD resin is then capped and sealed to maintain the resin in itshydrated form.

Resin SelectionXAD-4, XAD-7, XAD-16,XAD-1180, XAD-2000, andXAD-2010 differ in poresize, surface area, and chemical composition. Select a pore size largeenough to allow the analyteto enter the pore and adsorbonto the pore surface.

ProductSelection

Resin CharacteristicsComposition Polarity Mesh Size Pore Dia. Å Surface Area

m2/grXAD-4 Hydrophobic

Polyaromatic ResinNon-Polar 20 - 60 40 725

XAD-7 Acrylic Resin Moderately Polar

20 - 60 90 450

XAD-1180 HydrophobicPolyaromatic Resin

Non-Polar 20 - 60 300 600


Non-Polar 20 - 60 42 580


Non-Polar 20 - 60 280 660


Non-Polar 20 - 60 100 800

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520



These analytical grade resins are prepared through a multi-solvent precleaningand rehydration. Each cartridge is slurry packedand the resins are shippedwet and ready to use.


200mg/3mL 50 2092

XAD-4

500mg/3mL 50 2093

500mg/6mL 30 2094

1000mg/6mL 30 2097

1000mg/12mL 20 2099


2g/12mL 20 5091

2g/35mL 20 5092


Bulk Material100g 3492

300mg/15mL 30 3443

500mg/15mL 30 3445

3g/12mL 20 5093

3g/35mL 20 5094

5g/35mL 20 5095

10g/60mL 12 5097

20g/60mL 12 5098

ProductSelection

Resin Characteristics

Composition PolarityMeshSize

PoreDiameter Å

Surface Aream2/gr


Non-Polar 20 - 60 40 725

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XAD-7These analytical grade resins are prepared through a multi-solvent precleaningand rehydration. Each cartridge is slurry packedand the resins are shippedwet and ready to use.


200mg/3mL 50 2192

500mg/3mL 50 2193

500mg/6mL 30 2196

1000mg/6mL 30 2197

1000mg/12mL 20 2199


2g/12mL 20 5191

2g/35mL 20 5192



300mg/15mL 30 3473

500mg/15mL 30 3475

3g/12mL 20 5193

3g/35mL 20 5194

5g/35mL 20 5195

10g/60mL 12 5197

20g/60mL 12 5198

ProductSelection



PoreDiameter Å

Surface Aream2/gr

XAD-7 Acrylic Resin Moderately Polar 20 - 60 90 450

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520





200mg/3mL 50 2292

500mg/3mL 50 2293

500mg/6mL 30 2296

1000mg/6mL 30 2297

1000mg/12mL 20 2299


2g/12mL 20 5291

2g/35mL 20 5292



300mg/15mL 30 3483

500mg/15mL 30 3485

3g/12mL 20 5293

3g/35mL 20 5294

5g/35mL 20 5295

10g/60mL 12 5297

20g/60mL 12 5298

ProductSelection



PoreDiameter Å

Surface Aream2/gr


Non-Polar 20 - 60 100 800

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200mg/3mL 50 3247

500mg/3mL 50 3248

500mg/6mL 30 3249

1000mg/6mL 30 3250

1000mg/12mL 20 3251


2g/12mL 20 3253

2g/35mL 20 3254



300mg/15mL 30 3261

500mg/15mL 30 3262

3g/12mL 20 3255

3g/35mL 20 3256

5g/35mL 20 3257

10g/60mL 12 3258

20g/60mL 12 3259

ProductSelection



PoreDiameter Å

Surface Aream2/gr


Non-Polar 20 - 60 300 600


XAD-2000

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


These analytical grade resins are prepared through a multi-solvent precleaningand rehydration. Each cartridge is slurry packedand the resins are shippedwet and ready to use.


200mg/3mL 50 3265

500mg/3mL 50 3266

500mg/6mL 30 3267

1000mg/6mL 30 3268

1000mg/12mL 20 3269


2g/12mL 20 3271

2g/35mL 20 3272



300mg/15mL 30 3279

500mg/15mL 30 3280

3g/12mL 20 3273

3g/35mL 20 3274

5g/35mL 20 3275

10g/60mL 12 3276

20g/60mL 12 3277

ProductSelection



PoreDiameter Å

Surface Aream2/gr


Non-Polar 20 - 60 42 580


4-14TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520




200mg/3mL 50 3283

500mg/3mL 50 3284

500mg/6mL 30 3285

1000mg/6mL 30 3286

1000mg/12mL 20 3287


2g/12mL 20 3289

2g/35mL 20 3290



300mg/15mL 30 3297

500mg/15mL 30 3280

3g/12mL 20 3291

3g/35mL 20 3292

5g/35mL 20 3293

10g/60mL 12 3294

20g/60mL 12 3295

ProductSelection



PoreDiameter Å

Surface Aream2/gr


Non-Polar 20 - 60 280 660

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Spe-ed Amide-2

Affinity for PhenolsPlant Extracts

Spe-ed Amide-2 readily extracts polar molecules from natural productmatrices making it an exceptional sorbent for preparing plant extracts.

Spe-ed Amide-2 has an exceptional affinity for phenolic moities.Rapid desorption occurs depending on the ability of a solvent to compete with the hydrogen bonds between Spe-ed Amide-2 and phenols.

Spe-ed Amide-2 is ideal for:! Botanical extracts

Remove taninsQuinones irriversibly boundNitro aromatics

! Biotech researchAnti-HIV activityRemove carboxylic acids

! Natural products investigationsPhenol<resorcin<phloroglucinPhenol>pyrocatechol>pyrogallol

Spe-ed Amide-2 is pre-packed in standard SPE cartridges ranging insize from 1mL to 60mL. The Spe-ed Amide-2 SPE cartridges can beextracted manually, with a vacuum manifold, or on an automated SPEinstrument. Individual samples can be manually processed by syringeusing Spe-ed Amide-2 packed in MiniSpe-ed and/or MiniSpe-ed Pluscartridges.

Spe-ed Amide-2

H-N C = O - - - - - - - H—O—

C = O - - - - - - - H-N

CH2 H2C

4-16TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Amide-2

Pre-packed dry. Description Qty/Box Catalog NumberStandard Spe-ed Cartridges100mg/1mL 100 4821

200mg/3mL 50 4822

Spe-ed Amide-2

500mg/3mL 50 4823

500mg/6mL 30 4824

1000mg/6mL 30 4825

1000mg/12mL 20 4827


2g/12mL 20 4828

2g/35mL 20 4830



3g/12mL 20 4829

3g/35mL 20 4831

5g/35mL 20 4832

10g/60mL 12 4833

20g/60mL 12 4834

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


PuriPON

Containing Spe-ed DEX for thepurification of proteins,oligonucleotides, andnucleic acids.

PuriPON Columns eliminate the need forcentrifugation and phenolextractions because thedenatured protein isretained on the top fritand the phenol isadsorbed to the gelmatrix.

PuriPON ColumnsReliable methods for preparing pure DNA and RNA are essential formolecular biology research. Newly synthesized oligonucleotides oftenneed to be purified, desalted or exchanged into new buffers.

PuriPON Columns are prepacked, gravity flow columns used for therapid purification of nucleic acids, oligonucleotides and proteins. Eachcolumn contains a medium grade of Applied Separations’ Spe-ed DEXgel filtration media packed between two polyethylene frits. The fritsalso protect the gel from running dry under gravitational buffer flow.

Spe-ed DEX is biologically inert, bead-formed, cross linked dextrangel. Molecules that cannot enter the porous beads because of theirlarge size are eluted first. The smaller molecules enter the beads andare eluted in order of decreasing size.

Applications include:! Small scale purifications! Desalting samples! Buffer exchange! Removal of phenol and denatured protein after nucleic acid

extraction! Purifying fluorescently labeled oligonucleotides prior to

sequencing

Advantages:! Easy to use - pre-packed and wetted - use right out of the box! Time saving - no lengthy column packing or equilibration steps! Reproducibility - pre-packed columns ensure reproducible results! High yields - sample recovery of at least 90%

4-18TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


PuriPON

Description Qty/Box Catalog NumberPuriPON-50.5mL / 0.9 x 2.8cm gel bed 20 8101

0.5mL /0.9 x 2.8cm gel bed 50 8102

PuriPON Columns

PuriPON-101mL / 1.3 x 2.7cm gel bed 20 8103

1mL / 1.3 x 2.7cm gel bed 50 8104

PuriPON-252.5mL / 1.5 x 5.0cm gel bed 20 8105

2.5mL / 1.5 x 5.0cm gel bed 50 8106

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Drugs of Abuse

For Drugs of AbuseSpe-ed Scan ABNSpe-ed Flow ABNSpe-ed Scan THCSpe-ed Flow THCSpe-ed Scan THC IISpe-ed Flow THC II

Spe-ed Scan ABNThese cartridges enable selective isolation and elution of Acid, Basicand Netural drugs of abuse in exceptionally clean extracts with highrecoveries, at NIDA specified cut-off levels for confirmation by GC or GC/MS. Isolate Amphetamines, Barbituates, Benzolyechonine,Cocaine, and Opiates.

Spe-ed Flow ABNSpe-ed Flow ABN cartridges are designed to allow direct addition ofwhole blood samples through a SPE cartridge.

Spe-ed Scan THCSpe-ed Scan THC cartridges provide enhanced recoveries of ∆-9-carboxy THC and offer reliable isolations from urine or plasmafor confirmation by GC or GC/MS.

Spe-ed Flow THCSpe-ed Flow THC cartridges are designed to allow for direct addtionof whole blood samples through a SPE cartridge.

Spe-ed Scan THC IIWhen cleaner extracts are needed, use Spe-ed Scan THC II. Thebifunctionality provides for greater consistency and a higher percentrecovery.

Spe-ed Flow THC IISpe-ed Flow THC II cartridges are designed to allow for direct addition of whole blood samples throu a SPE cartridge without clogging.

4-20TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Drugs of Abuse




Description Qty/Box Catalog NumberSpe-ed Scan ABN3mL 50 2713

6mL 30 2714

for Drugs of Abuse

15mL LRC 30 3713

15mL LRC with cap* 30 3723Spe-ed Flow ABN3mL 50 2723

6mL 30 2724

15mL LRC 30 2725

15mL LRC with cap* 30 3725

Spe-ed Scan THC3mL 50 2613

6mL 30 3714

15mL LRC 30 3615


Spe-ed Flow THC3mL 50 2619

6mL 30 2620

15mL LRC 30 2621


Spe-ed Scan THC II3mL 50 2615

6mL 30 2616

15mL LRC 30 2617


Spe-ed Flow THC II3mL 50 2623

6mL 30 2624

15mL LRC 30 2625


SPE Catalog Section 5 Spe-ed eL/Lute Spe-ed DRI 5-1 Spe-ed eL/Lute 5-3 Spe-ed DRI


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Spe-ed eL/Lute

Liquid/LiquidExtraction on a Solid PhaseSupport

Spe-ed eL/LuteSpe-ed eL/Lute cartridges:! contain a special grade of high purity, inert diatomaceous earth! are packed in standard solid phase extraction tubes! incorporate a phase-separation filtering material to prevent

contamination of organic eluents by aqueous matrix! require gravity only

Efficient, Emulsion-free InterationsThe high surface area of the Spe-ed eL/Lute packing facititates efficient, emulsion-free interactions betwen the sample and the organic extraction solvent..

Selecting a Spe-ed eL/Lute CartridgeSelection of the correct Spe-ed eL/Lute cartridge is based upon thevolume of your aqueous sample. Use a cartridge of equal or greatercapacity than your sample volume. Capacity of the Spe-ed eL/Lutepacking is specified in the cartridge description.

Applied Separations’Spe-ed eL/Lute disposableliquid/liquid extraction cartridges simplify and speed up multiple liquid/liquid extractions.

12

9

6

3

Step 1:Apply

Aqueous Sample Step 3:

Add OrganicExtraction

Solvent

Step 2: Wait 3-5 Minutes

SampleRetentionArea

Sample/SolventInterface

CollectPurifiedExtractEmpty

eL/Lute with Spe-ed Matrix

5-2TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed eL/Lute

Description Qty/Box Catalog NumberSample Capacity0.3mL 100 2180

1mL 100 2181

3mL 100 2182

3mL pH 9.0 100 2183

3mL pH 4.5 100 2184

5mL 100 2185

10mL 100 2186

20mL 100 2187

50mL 50 2188

100mL 25 2189

15 2190300mL

Liquid/LiquidExtraction on a Solid PhaseSupport

Spe-ed eL/Lute

Applied Separations’Spe-ed eL/Lute disposableliquid/liquid extraction cartridges simplify and speed up multiple liquid/liquid extractions.

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed DRI

Drying Cartridge - Remove water fromsamples and solutions.

Spe-ed DRISpe-ed DRI - The Fast and Effective Way to Remove Waterfrom Samples and Solutions

Spe-ed DRI disposible cartridges are a family of car-tridges containing a variety of drying agents. The lasttraces of moisture can be removed from an organic solu-tion by passing it through a Spe-ed DRI Cartridge.

ChromagrographyNegate possible interferances or reactions in your chro-matorgaphic work with Spe-ed DRI. Save your expen-sive HPLC and GC columns from shortened life bywater damage. This water guard is an inexpensive pre-caution.

Irreversibly Binds WaterBecuase your samples have different characteristics, youmay need different drying agents to give you the bestresults. To meet these needs, Applied Separations has avariety of drying agents available.

Spe-ed DRI is Easy to Use

Manual SyringePlace the Spe-ed DRI on the end of your dispensing device or on the

male luer fitting of a manual syringe.Run the liquid through the Spe-ed DRI.Push air or gas through the cartridge to remove as much liquid as possible.

Vacuum ManifoldPlace the Spe-ed DRI cartridges on your vacuum manifold.Place your collection glassware in the vacuum manifold.Introduce the liquid into the Spe-ed DRI cartridge and draw vacuum.Draw vacuum until as much liquid as possible has been extracted from

the cartridge bed.Remove your glassware containing the “dried” liquid and continue

further sample processing.

H2O

H20H20

H20

H20H20

H20H20

Spe-ed DRI

Description Qty/Box Catalog NumberSpe-ed DRI-1MiniSpe-ed 50 14500

Generic SPE Cartridge 50 14530

Spe-ed DRI

Spe-ed DRI-2MiniSpe-ed 50 14510

Generic SPE Cartridge 50 14520Spe-ed DRI-3MiniSpe-ed 50 14511


Spe-ed DRI-4MiniSpe-ed 50 14512


5-4TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed DRI-1Very mild, slow, high capacity; good for gross pre-drying, but do notwarm the solution. Contains NaSO4

1.

Spe-ed DRI-2Excellent general agent; very inert but may be slightly acidic (avoidwith very acid-sensitive compounds). May be soluble in some organicsolvents. Contains MgSO4.

Spe-ed DRI-3Very efficient and adsorbs H2O; good for drying polar liquids (pre-drying with Spe-ed DRI-1 or Spe-ed DRI-2 suggested).Contains alkali alumina silicate.

Spe-ed DRI-4Very efficient and adsorbs H2O, EtOH; good for drying non-polar liquids (pre-drying with Spe-ed DRI-1 or Spe-ed DRI-2suggested). Contains alkali alumina silicate.

SPE Catalog Section 6 Spe-ed Flash Flangeless Cartridges 6-1 Spe-ed Flash 6-2 Spe-ed Flash D12 6-3 Spe-ed Flash D40 6-5 Spe-ed Flash D65 6-6 Spe-ed Flash Accessories 6-7 Spe-ed Flangeless Cartridges


TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Flash Chromatrography Columns

Disposible Columnsfor FlashChromatography

Spe-ed FLASH cartridges:Spe-ed FLASH columns have been designed and manufactured tomeet the critical needs of researchers who are using automated organicpurification insturments.

Consistent ResultsApplied Separations’ Flash Columnsprovide uniformity from batch tobatch.

Uniform, Fast FlowObtain excellent separations due tothe narrow particle size distributionwith Applied Separations’ FlashColumns.

No Pressure ProblemsNever lose another sample due to pressure problems. Every cartridgeis tested for leaks at 100 psi.

Buy Your FlashColumns from aCompany that

ActuallyManufactures the

Packings

6-2TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


-12Flash Chromatrography Columns

Description Qty/Box Catalog NumberSilica GelSiD12S 20 12347

SiD12M 20 12348

SiD12S 100 12349

SiD12M 100 12350C18/18%C18/18D12S 20 12353

C18/18D12M 20 12354

C4C4D12S 20 12357

C4D12M 20 12358

SCXSCXD12S 12 12361

SCXD12M 12 12362

SAXSAXD12S 20 12364

SAXD12M 20 12365HP20SSHP20SSD12S 20 12367

HP20SSD12M 20 12368

Alumina (neutral)ALND12S 20 12394

ALND12M 20 12395



Description Qty/Box Catalog NumberSilica GelSiD40S 12 12301

SiD40S 144 12302

SiD40M 12 12303

SiD40M 144 12304

SiD40L 12 12305

SiD40L 144 12306

SiD40XL 12 12307

SiD40XL 144 12308C18/18%C18/18D40S 1 12309

C18/18D40S 12 12310

C18/18D40M 1 12311

C18/18D40M 12 12312

C18/18D40L 1 12313

C18/18D40L 12 12314

C18/18D40XL 1 12315

C18/18D40XL 12 12316

C4C4D40S 1 12317

C4D40S 12 12318

C4D40M 1 12319

C4D40M 12 12320

C4D40L 1 12321

C4D40L 12 12322

C4D40XL 1 12323

C4D40XL 12 12324

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520



-40


6-4TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Description Qty/Box Catalog NumberSCXSCXD40S 1 12260

SCXD40S 12 12261

SCXD40M 1 12262

SCXD40M 12 12263

SCXD40L 1 12264

SCXD40L 12 12265

SCXD40XL 1 12266

SCXD40XL 12 12267SAXSAXD40S 1 12268

SAXD40S 12 12269

SAXD40M 1 12270

SAXD40M 12 12271

SAXD40L 1 12272

SAXD40L 12 12273

SAXD40XL 1 12274

SAXD40XL 12 12275

HP20SSHP20SSD40S 1 12276

HP20SSD40S 12 12277

HP20SSD40M 1 12278

HP20SSD40M 12 12279

HP20SSD40L 1 12280

HP20SSD40L 12 12281

HP20SSD40XL 1 12282

HP20SSD40XL 12 12283


-40


TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


-65Description

Silica GelSiD65M 6 12425

SiD65L 6 12426

C18/18%C18/18D65M 6 12428

C18/18D65L 6 12429

C4C4D65M 6 12431

C4D65L 6 12432

SCXSCXD65M 6 12434

SCXD65L 6 12435


SAXSAXD65M 6 12437

SAXD65L 6 12438HP20SSHP20SSD65M 6 12440

HP20SSD65L 6 12441

6 12443

6 12444

Alumina (neutral)ALND65M

ALND65L


6-6TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Description Qty/Box Catalog NumberD12 SeriesD12S Cartridge Assembly 50 12060

D12M Cartridge Assembly 50 12063

20µ PE Frit 100 12062

Cap 100 12061

D65 SeriesD65M Cartridge Assembly 50 00000

D65L Cartridge Assembly 50 00000

20µ PE Frit 50 12062

Cap 50 12061

D40 SeriesD40S Cartridge Assembly 50 12066

D40M Cartridge Assembly 50 12070

D40L Cartridge Assembly 50 12071

D40XL Cartridge Assembly 50 12072

20µ PE Frit 50 12068

Cap 50 12067

Tip 50 12069


D12 Series

D40/65 Series

Applied Separations’ FlashColumns can be purchasedeither empty or with frits.Separate frits are also available in order to meetindividual extraction needs.These accessories, used individually or in combination, result in customized extractioncolumns for use in flashchromatography procedures.

CAP

CAP

COLUMN

FRIT

FRIT

CAP

COLUMN

FRIT - INCLUDED

TIP

FRIT - OPTIONAL


Flangeless SPECartridges

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Today’s fully automated SPE processing systems requirean SPE cartridge configuration that has been speciallyadapted to fit these instruments. Applied Separations’Flangeless cartridges do not have the flange, or tab that isfound at the top of Applied Separations’ Standard SPEcartridge.

They are identical in every other way.

Flangeless Standard Applied Separations providesthe same wide variety and

high quality packings in thesespecially modified cartridgesas available in its standard

SPE cartridges.

The flangeless design allows for closer packing in specialized automated SPE systems, such as the Gilson® SPE215. This format will accommodate 96 x 1mL cartridges, or 48 x 3mL cartridges in the standard 96-well footprint.

If non-standard sorbents, packing amounts, or cartridge sizes are required, Applied Separationscan provide them.

Empty cartridges with a wide variety of different fritmaterial are also available.

Flangeless


Flangeless SPECartridges

Sorbent packed in speciallydesigned 1mL, 3mL, 6mL,or 12mL polypropylene cartridge syringe barrels.

Flangeless

6-8TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Sorbent Catalog NumberFlangeless CartridgesC18 Octadecyl C18/14%




13100

C8 Octyl

PH Phenyl

CH Cyclohexyl

C4 Butyl

C2 Ethyl

C1 Methyl


CN Cyanopropyl

SI Silica Gel

FLO Florisil



ALB Alumina (Basic)

DIO Diol



NH2 Aminopropyl

PSA Pri/Sec Amino

DEA Diethylamino

N+ Quaternary Amino

100mg1mL

13101

200mg3mL

13102

500mg3mL

13103

500mg6mL

13104

1000mg6mL

13281

13106 13107 13108 13109 13110 13282

13112 13113 13114 13115 13116 13283

13118 13119 13120 13121 13122 13284

13124 13125 13126 13127 13128 13285

13130 13131 13132 13133 13134 13286

13136 13137 13138 13139 13140 13287

13142 13143 13144 13145 13146 13288

13148 13149 13150 13151 13152 13289

13154 13155 13156 13157 13158 13290

13160 13161 13162 13163 13164 13291

13166 13167 13168 13169 13170 13292

13172 13173 13174 13175 13176 13293

13178 13179 13180 13181 13182 13294

13184 13185 13186 13187 13188 13295

13190 13191 13192 13193 13194 13296

13196 13197 13198 13199 13200 13297

13202 13203 13204 13205 13206 13298

13208 13209 13210 13211 13212 13299

13214 13215 13216 13217 13218 13280

13220 13221 13222 13223 13224 13300

13226 13227 13228 13229 13230 13301

13232 13233 13234 13235 13236 13302

13238 13239 13240 13241 13242 13303

Qty/Box 100 50 50 30 30 30

2g6mL

13105

13111

13117

13123

13129

13135

13141

13147

13153

13159

13165

13171

13177

13183

13189

13195

13201

13207

13213

13219

13225

13231

13237

13243

20

1000mg12mL

SPE Catalog Section 7 Method Development Kits 7-1 Non-Polar Kits 7-2 Moderate Non-Polar Kits 7-3 Weak Non-Polar Kits 7-4 Polar Kits 7-6 Ion Exchange Kits 7-8 RP Resins 7-10 Amberlite® XAD


Spe-ed Method Development Kits

Each Method DevelopmentKit contains a sampling ofsorbents for developing and optimizing samplepreparation for methods and procedures.

Custom Kits are also available. These kits contain varying sizes and combinations. Please inquire.

Description Qty/Box Catalog Number100mg/1mL 48C18 Octadecyl/14 12

2550

C18 Octadecyl/18 12

Non-Polar

C18 Octadecyl/22 12

C18 Octadecyl/OH 12

500mg/3mL 40C18 Octadecyl/14 10

2501

C18 Octadecyl/18 10

C18 Octadecyl/22 10

C18 Octadecyl/OH 10500mg/6mL 32C18 Octadecyl/14 8

2551

C18 Octadecyl/18 8

C18 Octadecyl/22 8


2552

C18 Octadecyl/18 8

C18 Octadecyl/22 8


2553

C18 Octadecyl/18 4

C18 Octadecyl/22 4

C18 Octadecyl/OH 45g/20mL 16C18 Octadecyl/14 4

2554

C18 Octadecyl/18 4

C18 Octadecyl/22 4

C18 Octadecyl/OH 4

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


7-2TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520





Description Qty/Box Catalog Number100mg/1mL 36C8 Octyl 12

2557

PH Phenyl 12

Moderate Non-Polar

CH Cyclohexyl 12

500mg/3mL 50C8 Octyl 20

2502

PH Phenyl 20

CH Cyclohexyl 10


2558

PH Phenyl 8

CH Cyclohexyl 8


2559

PH Phenyl 8

CH Cyclohexyl 8


2560

PH Phenyl 4

CH Cyclohexyl 4

5g/20mL 12C8 Octyl 4

2561

PH Phenyl 4

CH Cyclohexyl 4

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520





Description Qty/Box Catalog Number100mg/1mL 48C4 Butyl 12

2564

C2 Ethyl 12

Weak Non-Polar

C1 Methyl 12

CNe Cyanopropyl (endcapped) 12500mg/3mL 50C4 Butyl 10

2504

C2 Ethyl 10

C1 Methyl 10


2565

C2 Ethyl 8

C1 Methyl 8


2566

C2 Ethyl 8

C1 Methyl 8


2567

C2 Ethyl 4

C1 Methyl 4

CNe Cyanopropyl (endcapped) 45g/20mL 16C4 Butyl 4

2568

C2 Ethyl 4

C1 Methyl 4

CNe Cyanopropyl (endcapped) 4

7-4TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520





Description Qty/Box Catalog Number100mg/1mL 84CN Cyanopropyl 12

2571

SI Silica 12

Polar

FLO Florisil® 12

ALN Alumina (Neutral) 12

ALA Alumina (Acidic) 12

ALB Alumina (Basic) 12

DIO (Diol) 12500mg/3mL 50CN Cyanopropyl 10

2505

SI Silica 10

FLO Florisil® 10





2572

SI Silica 8

FLO Florisil® 8




DIO (Diol) 8


TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520





Description Qty/Box Catalog Number1000mg/6mL 56CN Cyanopropyl 8

2573

SI Silica 8

Polar

FLO Florisil® 8





2574

SI Silica 4

FLO Florisil® 4




DIO (Diol) 45g/20mL 28CN Cyanopropyl 4

2575

SI Silica 4

FLO Florisil® 4




DIO (Diol) 4


7-6TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520





Description Qty/Box Catalog Number100mg/1mL 72COOH Carboxylic Acid 12

2578

SCX Benzenesulfonic Acid 12

Ion-Exchange

NH2 Aminopropyl 12

PSA Primary/Secondary Amine 12

DEA Diethylamino 12

N+ Quaternary Amino 12500mg/3mL 50COOH Carboxylic Acid 10

2506


NH2 Aminopropyl 10


DEA Diethylamino 5


2579


NH2 Aminopropyl 8


DEA Diethylamino 8


2578


NH2 Aminopropyl 8


DEA Diethylamino 8

N+ Quaternary Amino 8

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520





Description Qty/Box Catalog Number1000mg/12mL 24COOH Carboxylic Acid 4

2581


Ion-Exchange

NH2 Aminopropyl 4


DEA Diethylamino 4

N+ Quaternary Amino 42.5g/20mL 24COOH Carboxylic Acid 4

2582


NH2 Aminopropyl 4


DEA Diethylamino 4

N+ Quaternary Amino 45g/20mL 24COOH Carboxylic Acid 4

2583


NH2 Aminopropyl 4


DEA Diethylamino 4

N+ Quaternary Amino 4

7-8TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520



RP101, 102, 103, 104, 105



Description Qty/Box Catalog Number100mg/1mL 60RP-101 12

2585

RP-102 12

RP Resins

RP-103 12

RP-104 12

RP-105 12500mg/3mL 50RP-101 10

2587

RP-102 10

RP-103 10

RP-104 10

RP-105 10500mg/6mL 40RP-101 8

2589

RP-102 8

RP-103 8

RP-104 8

RP-105 81000mg/12mL 20RP-101 4

2591

RP-102 4

RP-103 4

RP-104 4

RP-105 42.5g/20mL 20RP-101 4

2593

RP-102 4

RP-103 4

RP-104 4

RP-105 4

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520



RP101. 102, 103, 104



Description Qty/Box Catalog Number100mg/1mL 48RP-101 12

2586

RP-102 12

RP Resins

RP-103 12

RP-104

RP-101

RP-102

RP-103

RP-104

RP-101

RP-102

RP-103

RP-104

RP-101

RP-102

RP-103

RP-104

RP-101

RP-102

RP-103

RP-104

12

500mg/3mL 4010

2588

10

10

101000mg/6mL 48

122590

12

12

121000mg/12mL 16

42592

4

4

42.5g/20mL 16

42594

4

4

4

7-10TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520





Description Qty/Box Catalog Number500mg/6mL 35Amberlite® XAD-4

Amberlite® XAD-7

Amberlite® XAD-16

Amberlite® XAD-1180

® Amberlite is a registered trademark of Rohm and Haas Co.

102507

5

Amberlite® XAD

5

5

Amberlite® XAD-2000 5

Amberlite® XAD-2010 5

SPE Catalog Section 8 Accessories 8-1 Accessories 8-2 Empty Cartridges 8-3 Fritted Empty Cartridges 8-5 Filtered Empty Cartridges 8-7 Frits 8-13 Syringe Plungers

Stopcocks Universal Adaptors Caps Tips


TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Accessories

AccessoriesEmpty Cartridges and FritsApplied Separations offers a variety of Spe-ed SPE accessories, including medical grade polypropylene cartridges, frits, filters, syringeplungers, adaptors, caps, tips, and stopcocks. The polypropylene cartridges are available in sizes ranging from 1mL to 60mL, MiniSpe-ed(Sep-Pak™ type), and large reservoir cartridges (LRC). These cartridges can be purchased either empty or with frits.

FritsFrits of assorted materials and pore sizes are available to meet individual extraction needs.

Syringe PlungersSyringe Plungers are sold separately for manual extraction.

Customize SPE ProcedureAccessories are available to mix and match in order to customize SPEprocedure.

Adaptors

Tips

Caps

Empty cartridge with two frits.

Stopcocks

Frits

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


All cartridges are constructedof high grade polypropylene.Nominal cartridge volumesare listed, but actual volumeis greater.

Spe-ed Accessories

Description Qty/Box Catalog NumberStandard Spe-ed Cartridges1mL 100 2442

3mL 100 2443

Empty Cartridges

6mL 100 2417

12mL 100 2410

20mL 100 2453

35mL 100 2424

60mL 100 2425

LRC Cartridges100mg 100 2407

500mg 100 2408



Large Reservoir Cartriges (LRCs)The wide openings of both the 100mg and 500mg LRCsallow for sample or rinse volumes greater than thatallowed by the standard cartridge. The flared shapeaccommodates 15mL of sample without using additionalreservoirs or adaptors.

MiniSpe-ed/MiniSpe-ed Plus CartridgesMiniSpe-ed cartridges contain the wide variety of sorbents found in standard Spe-ed cartridges. The MiniSpe-ed configuration can be easily used inmanual syringe operations or on a vacuum manifold.Also, MiniSpe-ed cartridges are fully compatible with instruments such as the Millilab™.

Standard Extraction ColumnsStandard extraction columns are straightwalled syringe barrel tubes designed for

fast, easy, and exact samplepreparation. These cartridgesare available in 6 differentsizes ranging from 1mL to60mL.

8-2

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Accessories

PolypropyleneCartridge with onePolyethyleneHydrophobic 20µ Frit(traditional SPE use)All cartridges are constructedof high grade polypropylene.Nominal cartridge volumesare listed, but actual volumeis greater.

PolypropyleneCartridge with onePolyethyleneHydrophobic 70µ Frit


Description Qty/Box Catalog Number20µPE Frit1mL 100

100

100

100

100

100

100

100

100

100

100

2441

3mL 2413

Fritted Cartridges

6mL 2416

12mL 2419

20mL 2464

35mL 2435

60mL 2446

MiniSpe-ed 2447

MiniSpe-ed Plus 8447

100mg LRC 2448

500mg LRC 2409

70µPE Frit1mL 100

100

100

100

100

100

100

100

100

2449

3mL 2450

6mL 2452

12mL 2427

35mL 2455

60mL 2456

MiniSpe-ed 2457

100mg LRC 2458

500mg LRC 2459

8-4TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Accessories

PolypropyleneCartridge with oneHydrophobic Frit(traditional SPE use)

Description Qty/Box Catalog Number100µPE Frit1mL 100

100

100

100

100

100

100

100

100

2924

3mL 2925

Fritted Cartridges

6mL 2926

12mL 2927

35mL 2928

60mL 2929

MiniSpe-ed 2930

100mg LRC 2931

500mg LRC 2932

5µPTFE Frit1mL 100

100

100

100

100

100

100

100

100

2933

3mL 2934

6mL 2935

12mL 2936

35mL 2937

60mL 2938

MiniSpe-ed 2939

100mg LRC 2940

500mg LRC 2941

PolypropyleneCartridge with onePolyethyleneHydrophobic 70µ Frit


TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Accessories

PolypropyleneCartridge with Filter

Although frit material acts as a filter, sometimesadditional filtering materialmay be necessary. AppliedSeparations offers smallerpore size filters insertedbetween two frits whichremove fines, or sampleparticulates that may havepassed through the sorbentbed.

Description Qty/Box Catalog Number.45µPTFE Filter (PE backing) between two 5µ PTFE Frits1mL 100

100

100

100

100

100

100

100

100

2472

3mL 2415

Filtered Cartridges

6mL 2433

12mL 2473

35mL 2474

60mL 2475

MiniSpe-ed 2476

100mg LRC 2477

500mg LRC 2478

.45µCellulose Filter between two 20µ PE Frits1mL 100

100

100

100

100

100

100

100

100

2479

3mL 2414

6mL 2481

12mL 2482

35mL 2483

60mL 2484

MiniSpe-ed 2485

100mg LRC 2942

500mg LRC 2986Filter

Frits

8-6TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Accessories

PolypropyleneCartridge with Filter

Although frit material acts as a filter, sometimesadditional filtering materialmay be necessary. AppliedSeparations offers smallerpore size filters insertedbetween two frits whichremove fines, or sampleparticulates that may havepassed through the sorbentbed.

Description Qty/Box Catalog Number.45µ Nylon Filter between two 20µ PE Frits1mL 100

100

100

100

100

100

100

100

100

2943

3mL 2944

Filtered Cartridges

6mL 2945

12mL 2946

35mL 2947

60mL 2948

MiniSpe-ed 2949

100mg LRC 2950

500mg LRC 2951

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Accessories

Frit Selection Guide

Frits are friction fitted withinthe cartridge to retain the sorbent, and to perform avariety of filtration functions.

Applied Separations’ porous,plastic frits are naturallyhydrophobic, but aqueoussolutions can be forcedthrough under pressure.

If gravity flow of an aqueous solution is desired,select a specially treatedpolyethylene hydrophilic frit.

FritsPolyethylene Hydrophobic FritsPolyethylene hydrophobic frits are the standard and are used in mostcommercial SPE products. They are also used with non-aqueous solutions such as solvents, alcohols, acids, bases, halogenated and aromatic hydrocarbons, and ketones. These hydrophobic frits can bewetted with organic solvents.

Polyethylene Hydrophilic FritsPolyethylene hydrophillic frits are used for general filtration and as forclarification of aqueous solutions and certain other solutions. Thesehydrophilic frits are specially treated and can be wetted with aqueoussolutions. This frit is ideal for gravity flow of an aqueous solution.

PTFE and Stainless SteelPTFE and stainless steel frits can be used to filter almost any organicsolvent. They are used for trace analysis and high sensitivity workwith solvent detection systems which are not compatible with polyethylene or polypropylene frits. PTFE is a hydrophobic membraneresistant to most organic solvents, acids, and bases.

PolypropylenePolypropylene frits are hydrophobic and are best used for lowextractables. Polypropylene frits are similar to PTFE frits in their filtration behavior and serve as an economical alternative to the moreexpensive PTFE frits.

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520






FritsDescription Qty/Box Catalog Number20µ Hydrophobic Polyethylene Frits1mL 100

100

100

100

100

100

100

100

100

100

2421

3mL 2423

6mL 2426

12mL 2488

35mL 2431

60mL 2489

MiniSpe-ed 2491

MiniSpe-ed Plus 8491

100mg LRC 2492

500mg LRC 2451

70µ Hydrophobic Polyethylene Frits1mL 100

100

100

100

100

100

100

100

100

2493

3mL 2494

6mL 2495

12mL 2430

35mL 2496

60mL 2469

MiniSpe-ed 2468

100mg LRC 2424

500mg LRC 2425

Spe-ed Accessories

8-8

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Accessories





FritsDescription Qty/Box Catalog Number100µ Hydrophobic Polyethylene Frits1mL 100

100

100

100

100

100

100

100

100

2952

3mL 2953

6mL 2954

12mL 2955

35mL 2956

60mL 2957

MiniSpe-ed 2958

100mg LRC 2959

500mg LRC 2960

20µPE Hydrophilic Polyethylene Frits1mL 100

100

100

100

100

100

100

100

100

2961

3mL 2962

6mL 2963

12mL 2964

35mL 2965

60mL 2966

MiniSpe-ed 2967

100mg LRC 2968

500mg LRC 2969

8-10TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Accessories





FritsDescription Qty/Box Catalog Number70µPE Hydrophilic Polyethylene Frits1mL 100

100

100

100

100

100

100

100

100

2970

3mL 2971

6mL 2972

12mL 2973

35mL 2974

60mL 2975

MiniSpe-ed 2976

100mg LRC 2977

500mg LRC 2978

100µPE Hydrophilic Polyethylene Frits1mL 100

100

100

100

100

100

100

100

100

2979

3mL 2980

6mL 2981

12mL 2982

35mL 2983

60mL 2984

MiniSpe-ed 2985

100mg LRC 2986

500mg LRC 2987

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Accessories





FritsDescription Qty/Box Catalog Number5µ PTFE Frits1mL 100

100

100

100

100

100

100

100

100

2499

3mL 2901

6mL 2902

12mL 2903

35mL 2904

60mL 2905

MiniSpe-ed 2906

100mg LRC 2907

500mg LRC 2908

Stainless Steel Frits1mL 100

100

100

100

100

100

100

100

100

2915

3mL 2916

6mL 2917

12mL 2918

35mL 2919

60mL 2920

MiniSpe-ed 2921

100mg LRC 2922

500mg LRC 2923

8-12TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Accessories

Description Qty/Box Catalog Number150µ Polypropylene Frits1mL 100

100

100

100

100

100

100

100

100

2988

3mL 2989

6mL 2990

12mL 2991

35mL 2992

60mL 2993

MiniSpe-ed 2994

100mg LRC 2995

500mg LRC 2996





Frits

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Spe-ed Accessories

AccessoriesSyringe Plungers

Stopcocks

Univeral Adaptors

Cap Plugs

Tips

Description Qty/Box Catalog NumberSyringe Plungers

100

100

100

100

100

3mL 2910

6mL 2418

Stopcocks10

10

PTFE 2406

Nylon 2403

Universal Adaptors10One Size 2402

Tips100One Size 8050

12mL 2440

35mL 2441

60mL 2911

Cap Plugs100

100

100

100

100

1mL 8155

3mL 8054

6mL 8156

12mL 8157

35mL 8158

10060mL 8159

SPE Catalog Section 9 Spe-ed Mate Vacuum Manifolds 9-1 Stainless Steel Vacuum Manifolds 9-2 Glass Vacuum Manifolds 9-3 Clear Acrylic Vacuum Manifolds 9-4 AquaSafe Polycarbonate Vacuum Manifolds 9-5 Vacuum Manifold Accessories


TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Vacuum Manifolds

Stainless SteelVacuum Manifolds

Spe-ed Mate-30The Spe-ed Mate-30 manifold is the largest commercially available manifoldand holds up to 30 cartridges. It will hold 30 large reservoir cartridges, as wellas 30 standard cartridges ranging from 1mL to 60mL.

Spe-ed Mate-10The Spe-ed Mate-10 offers a compact option for preparation of up to 10 samples.

Special Features of the Spe-ed Mate Vacuum Manifold! Universal Collection Rack - Provides eluate collection in test tubes,

volumetrics, LSC vials, WISP cups, micro and standard centrifuge tubes, and other analytical containers. Custom racks are also available.

! Removable Luer-fitted Needles - A variety of sizes, lengths, and materials, including Teflon®, stainless steel, and polypropylene - compatible with most solvents, acids, and samples.

! Chemically Resistant Cover - Inhibits surface deterioration and contamination.

! Individual Control - With the addition of stopcocks, the simultaneous vacuum, extraction, elution, or filtration of samples can be controlled.

! Special Coating with Inert Polymer available - for long life and uninterrupted operation in very hostile and corrosive environments.

Spe-ed Mate

Spe-ed Mate-10Spe-ed Mate-30

Spe-ed Mate VacuumManifolds offer the largest range of options for processing samples for environmental, pharmaceutical, clinical, or biological analysis.

#1010 Spe-ed Mate-1010-position vacuum SPE processor with 5” x 7” basin;stainless steel collection tube rack for 20mL scintillation vials or 12mm x 75mm test tubes.

#1030 Spe-ed Mate-3030-position vacuum SPE processor with 10” x 12” basin;stainless steel collection rack for 10mm x 75mm to 16mm x 125mm test tubes and volumetric LSC vials.

9-2TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Vacuum Manifolds

Glass VacuumManifolds

Spe-ed Mate Glass Vacuum ManifoldSpe-ed Mate Glass Vacuum Manifolds offer the advantages of our standardSpe-ed Mates PLUS are an easily cleaned, chemically-inert, clear glass chamber for visually monitoring vacuum operations. Simultaneous vacuum,extraction, elution, or filtration of samples can be controlled for each valve.The individual vacuum gauge and bleed valve assembly allows control andmonitoring of vacuum applied to the chamber. Luer connections on the valveassemblies accept a broad range of SPE cartridge sizes from 1mL to 60mL.Adjustable racks inside the glass walled vacuum chamber accommodate a variety of collection vessels. Eluants are deposited directly into collection vessels via polypropylene needles.

Special Features of the Spe-ed Mate Glass Vacuum Manifold! Glass Vacuum Chamber - Chemically-inert 1/4” thick glass vacuum

chamber.! Adjustable/Interchangeable Racks - Adjustable racks accommodate a

variety of collection vessels.! Removable Luer-fitted Needles - Easy to insert polypropylene needles.! Available in three Sizes - Spe-ed Mate Glass Vacuum Manifold in 12, 16,

and 24 position sizes.

Spe-ed Mate

Glass Spe-ed Mate-12Glass Spe-ed Mate-16Glass Spe-ed Mate-24


TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Vacuum Manifolds

Clear AcrylicVacuum Manifolds

Spe-ed Mate Clear Acrylic Vacuum ManifoldSpe-ed Mate Clear Acrylic Vacuum Manifolds offer the same large range ofoptions available in our standard stainless Spe-ed Mates. It has the addedadvantage of being made of shatterproof, clear see-through acrylic. Available inboth 10-position, and 30-position vacuum processors, the Acrylic Spe-ed Matecan accommodate a wide variety of collection vessels.

Special Features of the Spe-ed Mate Glass Vacuum Manifold! Glass Vacuum Chamber - Chemically-inert 1/4” thick glass vacuum

chamber.! Adjustable/Interchangeable Racks - Adjustable racks accommodate a

variety of collection vessels.! Removable Luer-fitted Needles - Easy to insert polypropylene needles.! Available in three Sizes - Spe-ed Mate Glass Vacuum Manifold in 12, 16,

and 24 position sizes.

Spe-ed Mate

Spe-ed Mate-10Spe-ed Mate-30


Vacuum Manifolds

PolycarbonateVacuum Manifolds

Spe-ed Mate AquaSafe Vacuum ManifoldsOriginally designed for the nuclear industry, Spe-ed Mate AquaSafe VacuumManifolds are practical for any aqueous or acidic solid phase extraction.Constructed of polycarbonate, AquaSafe vacuum manifolds are up to 250 times more impact resistant than glass manifolds. Unlike stainless steel manifolds, AquaSafe products resist acids.*

AquaSafe

Spe-ed Mate-20 AquaSafeSpe-ed Mate-16 AquaSafeSpe-ed Mate-16jr AquaSafe

9-4TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


*AquaSafe products should not be used with organic solvents.

Vacuum Manifolds

Spe-ed Mate-30Rack System

Spe-ed Mate-30 Rack SystemCat. No. Product Description Qty.

1023 Tube Collection Rack 1Stainless steel collection rack for Spe-ed Mate-30 consists of 4 support post assemblies and 2 plates. (Specify upper and lower plates as required.)

1021 Support Post Assembly 4Each stainless steel assembly contains 1 support post, 2 upper plate adjustment nuts, and 2 lower plate adjustment nuts.

1025 Plate A: 13mm hole diameter 1Fits on support post for collection into 10mm x 75mm and 12mm x 75mm tubes, autosampler vials, micro-centrifuge tubes, and similar glassware.

1026 Plate B: 17mm hole diameter 1Fits on support post for collection into 16mm x 125mm or 16mm x 100mm tubes, centrifuge tubes, and similar glassware.

1027 Plate C: 8mm hole diameter 1Fits on support post and is used for micro-centrifuge tubes or similar glassware.

1028 Plate D 1Fits on support post for collection in 1, 2, 5, and 10mL volumetric flasks and similar glassware.

Accessories

TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


9-6TO ORDER CALL (610) 770-00900 • FAX (610) 740-55520


Vacuum Manifolds

Accessories andReplacements

AccessoriesAccessories and ReplacementsCat. No. Product Description Qty.

1033 Luer-fitted Needles - Stainless Steel 301036 Luer-fitted Needles - Flexible polypropylene 1-1/2” 301039 Luer-fitted Teflon® Needles 302402 Universal Adapters 10

Fit 1, 3, and 6mL Spe-ed Cartridges2403 One-way Nylon Stopcocks 102406 One-way Teflon® Stopcocks 101034 Luer-fitted Plugs for Spe-ed Mate-30 Basin 301032 Spe-ed Mate-30 Gaskets 31035 Spe-ed Mate-30 Cover 11012 Spe-ed Mate-10 Gaskets 31011 Spe-ed Mate-10 Collection Rack 11013 Spe-ed Mate-10 Cover - Polypropylene 11015 Vacuum Gauge/Controller 1

Maintains and releases up to 15 in. Hg Vacuum1024 Teflon® coating

for Spe-ed Mate-10 Basin and Racks 1

Inquire about Teflon coating for Spe-ed Mate-30 Basin and Racks.

SPE Aplikace

ENVIRONMENTAL APPLICATIONS APP#

APP101 Isolation Of Phenolic Compounds From Aqueous Solutions APP103 Isolation Of Chromium, Manganese, Iron, Cobalt, Nickel, Cooper & Zinc From Aqueous Solutions APP105 Isolation Of Amine Antioxidants From Motor Oil APP108 Isolation Of Organochlorine Pesticides From Fat Soluble Material APP109 Isolation Of Phenoxy Acid Herbicides From Aqueous Solutions APP110 Isolation Of Pesticides From Fish Oil APP111 Isolation Of PCB’s And PAH’s From Water APP112 Isolation Of PCB’s And PAH’s From Soil APP123 Isolation Of Organochlorine Pesticides From Aqueous Solutions APP124 Isolation Of Organochlorine Pesticides From Soil Or Low Fat Tissue APP127 Isolation Of PCB’s From Transformer Oil APP137 Isolation Of PCB’s And PAH’s From Whole Blood, Serum, Or Plasma APP166 Isolation Of Atrazine And Alachlor In Water APP167 Isolation Of Carbaryl From Water APP168 Isolation Of Carbaryl From Fruit Juice APP169 Isolation Of Phenylurea Herbicides From Water By LC/MS APP170 Isolation Of Aldicarb Residues From Ground Water APP171 Isolation Of Triazine Herbicides From Pond Water APP172 Isolation Of Organophosphorus Pesticides From Water APP181 Isolation Of Phenol And Phenol Compounds From Water APP184 Isolation Of Methiocarb From Fruit APP187 Isolation Of Phenols From Aqueous Samples APP188 Isolation Of Paraquat And Diquat From Aqueous Solutions APP189 Isolation Of Benomyl From Aqueous Solutions APP191 Isolation Of Atrazine From Tissue APP192 Isolation Of Atrazine From Soybeans APP193 Extraction Of Metals For SW846 APP194 Isolation Of Phenols From Aqueous Solutions APP201 Isolation Of Explosives From Water APP206 Clean-Up Of PCB’s From Transformer And Waste Oil APP208 Isolation Of Petroleum Products From Water APP220 Isolation Of Organophosphorous Insecticides From Water APP240 Isolation Of Triazine Herbicides From Water APP241 Isolation Of Triazine Herbicides From Soil APP250 Isolation Of PCB’s From Oil

BIOLOGICAL APPLICATIONS APP#

APP165 Isolation Of Adenosine Monophosphate From Lung Tissue APP186 Deproteination Of Blood, Urine, Or Cerebral Spinal Fuid From Amino Acid Testing

AGRICULTURAL / FOOD APPLICATIONS APP#

APP106 Isolation Of Aflatoxins From Grain, Corn And Nut Meals APP126 Isolation Of Caffeine From Aqueous Solutions APP139 Isolation Of Carboydrates From Wine APP175 Isolation Of Hop Acids From Beer APP179 Isolation Of Trimethoprim From Meat APP184 Isolation Of Methiocarb From Fruit APP191 Isolation Of Atrazine From Tissue APP192 Isolation Of Atrazine From Soybeans APP203 Isolation Of Caffeine And Aspartame From Coca-Cola APP207 Isolation Of Flunixin From Milk APP214 Isolation Of Tetracyclines From Honey APP215 Isolation Of Trace Caffeine From Aqueous Solutions APP222 Isolation Of Organochlorine Pesticides From Corn Meal APP223 Isolation Of Organochlorine Pesticides From Corn Meal APP224 Isolation Of Triazine Herbicides From Plant Extracts

TOXICOLOGY APPLICATIONS APP#

APP131 Isolation Drugs Of Abuse Urine APP176 Isolation Of Chlorinated Hydrocarbon Pesticides From Serum Or Plasma APP177 Isolation Of Anabolic Steroids From Urine And Serum APP180 Isolation Of Acidic, Basic, And Neutral Doping Agents From Equine And Canine Urine APP182 Isolation Of Amphetamines From Urine APP225 Isolation Of Barbiturates From Urine APP226 Isolation Of Cocaine And Benzoylecgonine From Urine APP227 Isolation Of Acidic, Neutral And Basic Drugs From Urine APP228 Isolation Of Acidic, Neutral And Basic Drugs From Whole Blood APP229 Isolation Of Basic Drugs From Urine APP231 Isolation Of Δ 9-Carboxy THC From Urine APP235 Isolation Of Phencyclidine APP236 Isolation Of Exogenous Compounds APP251 Isolation Of Opiates From Urine By Enzymatic Hydrolysis

ORGANIC APPLICATIONS APP#

APP173 Isolation Of Plant Pigments From Leaf Extracts APP174 Isolation Of Isoquinoline Alkaloids From Leaf Extracts APP183 Isolation Of Organic Acids From Aqueous Solutions APP198 Isolation Of Organic Acids From Sea Water

PHARMACEUTICAL APPLICATIONS APP#

APP102 Isolation Of Carboxylic Acids From Aqueous Solutions APP104 Isolation Of Carboxylic Acids From Water APP107 Isolation Of Parabens From Pharmaceuticals And Cosmetics APP113 Isolation Of Cyclosporin From Serum APP114 Isolation Of Diazepam From Serum APP115 Isolation Of Benzodiazepines From Serum APP116 Isolation Of Acetaminophen From Serum APP117 Isolation Of Pheophylline From Serum APP118 Isolation Of Barbiturates From Serum APP119 Isolation Of Propranolol From Serum APP120 Isolation Of Nadolol From Serum APP121 Isolation Of Prostaglandins And Other Arachidonic Acid Metabolites APP122 Isolation Of Steroids From Serum Or Urine APP125 Isolation Of Amino Acids From Aqueous Solutions APP128 Isolation Of Benzodiazepines From Urine APP129 Isolation Of Nicotine And Cotinine From Plasma APP130 Isolation Of Vitamin D3 And Its Metabolites From Serum Or Plasma APP132 Isolation Of Vitamins A,D, And E From Thetagran And Theragran M APP133 Isolation Of Tricyclic Antidepressants From Serum APP134 Isolation Of Phenothiazines From Serum APP135 Isolation Of Vitamins A,D,E,K, And Beta Carotene From Centrum Tablets APP136 Isolation Of Beta Carotene From Multi-Vitamins Tablet Extracts Of Vitamins A,D,E & K APP138 Isolation Of Baclofen From Serum APP140 Sample Preparation For Tricyclic Antidepressants For Analysis By Normal Phase HPLC APP141 Isolation Of Catecholamines From Urine APP142 Isolation Of Bambuterol From Plasma APP143 Isolation Of Haloperiod And Reduced Haloperidol From Plasma APP144 Isolation Of Amoxicillin From Human Plasma APP145 Isolation Of Aminoglycosides From Serum APP146 Isolation Of Propranolol From Plasma APP147 Isolation Of Pheytoin From Serum For GC APP148 Isolation Of Primidone From Serum For GC APP149 Isolation Of Carbamazepine From Serum For GC APP150 Isolation Of Phenobarbital From Serum For GC APP151 Isolation Of Anticonvulsants From Serum For GC APP152 Isolation Of Carbamazepine From Serum For HPLC APP153 Isolation Of Dilatin From Serum For HPLC APP154 Isolation Of Phenobarbital From Serum For HPLC APP155 Isolation Of Primidone From Serum For HPLC

APP156 Isolation Of Anticonvulsants From Serum For HPLC APP157 Isolation Of Antineoplastic Agents From Bisantrene And Mitoxantrone From Plasma APP158 Isolation Of Amiloride From Urine / Serum APP159 Isolation Of Atenolol From Human Plasma APP160 Isolation Of Mesoridazine From Serum APP161 Isolation Of Sulforidazine From Serum APP162 Isolation Of Thioridazine From Serum APP163 Isolation Of Antipsychotic* Drugs From Serum APP164 Isolation Of Amikacin From Serum APP178 Isolation Of Fat Soluble Vitamins From Multivitanim Tablets APP185 Isolation Of Thiamine From Aqueous Samples APP190 Isolation Of Cotinine From Urine And Plasma APP196 Isolation Of Arachidonic Acid Metabolites And Other Essential Fatty Acids From Urine APP197 Isolation Of Benzodiazapines From Serum For HPLC Analysis APP199 Isolation Of Catecholamines From Plasma APP202 Isolation Of Enkephalins From Urine APP205 Isolation Of Lidocaine From Serum APP211 Isolation Of Steroids From Serum APP212 Isolation Of Steroids From Serum APP213 Isolation Of Sulfonamides From Tissue APP217 Isolation Of Δ 9-Carboxy THC From Serum APP218 Isolation Of Cyclosporin A From Whole Blood APP219 Isolation Of Amoxicillin From Plasma APP221 Isolation Of Sulfonamides In Serum And Tissue APP233 Isolation Of Water Soluble Vitamins From Tablets APP239 Isolation Of Benzodiazepines From Serum From HPLC Analysis APP242 Isolation Of Ethacrynic Acid From Plasma / Urine Antidepressants APP244 Isolation Of 11 CIS, 13CIS, 9 CIS, And All Trans Retinol From Serum APP245 Isolation Of 9 CIS, 11 CIS, 13 CIS, And All Trans Retinoic Acid From Serum APP247 Isolation Of Ethchlorvynol APP248 Isolation Of Thyroxine From Aqueous Samples APP249 Isolation Of Thyroxine From Aqueous Solutions

Jednotlivé aplikace Vám na požádání zašleme

LABICOM s.r.o. Rokycanova 18, 779 00 Olomouc

Tel./Fax.: 585436915/16 [email protected] www.labicom.cz

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LABICOM s.r.o. VTP UP Šlechtitelů 19 783 71 Olomouc Česká republikaLABICOM s.r.o. VTP UP Šlechtitelů 19 783 71 Olomouc Česká republika

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VTP UP, Šlechtitelů 19, 783 71 Olomouc email: [email protected], www. labicom.cz Tel.: +420 585 436 915, Fax.: +420 585 436 916

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mailto:[email protected]

http://www.labicom.cz

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