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Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

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Page 1: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Microscopes

help us see small objects typically less than 500 nm

nm = nanometer = 1/1,000,000 mmsize of a human eggsize of a sharp pencil point

Page 2: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Magnification making an image appear larger than its actual

sizenot actually changing the size of the object

Page 3: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Magnification indicated by _____ X

i.e. an object that is 100X had been magnified 100 times

the bottom image is 1860X

Page 4: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Resolution

the ability to distinguish the details of an image

measure of the clarity of an image

Page 5: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Resolution

improved by adjusting the focus of a microscope

resolution capability of a microscope of may be determined by an object’s size and degree of detail

Page 6: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Resolution

often displayed as a distancea smaller distance means that a smaller object

can be viewed clearlya smaller distance means the microscope has

better resolution

Page 7: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Magnification and Resolution

Both high magnification and good resolution are necessary to see small objects clearly.

Page 8: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Types of Microscopes

1. Compound Light Microscopes

2. Electron Microscopes

Page 9: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

1. Compound Light Microscopes (CLM)

compoundmore than 1

lightilluminating the object

for clarity

light passes through two lenses to produce an enlarged image of a specimen

Page 10: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

1. Compound Light Microscopes (CLM)

What is the possible compounded magnification of these lenses?

magnification of an object observed using this microscope is the multiplication of the 2 (or more) lenses used to view the object

Page 11: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

1. Compound Light Microscopes (CLM)

Page 12: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

2. Electron Microscopes

microscopes shoot electrons at dead objects/specimens

as the electrons bounce back, the energy is recorded and translated into an image

Page 13: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

2. Electron Microscopes must be

viewed in a vacuumvacuum

○ no airno living cells

can be viewed

Page 14: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

2. Electron Microscopes

There are two types of Electron Microscopes1. Transmission electron microscope (TEM)

2. Scanning electron microscope (SEM)

Page 15: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Transmission Electron Microscope (TEM)

Form 2D images

TEM images are always in black and white.

with the help of computers scientists add color to make things easier to see

Page 16: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Transmission Electron Microscope (TEM)

An electron beam is directed at a slice of a specimen that has been stained with metal ions.

Some structures become more heavily stained than others. Those heavily stained areas absorb electrons and in the lightly stained areas the electrons pass through hitting a fluorescent screen and forming an image on the screen.

Page 17: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Transmission Electron Microscope (TEM)

Page 18: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Scanning Electron Microscope (SEM)

essentially works the same as a TEM (electron beams)

shows 3D images of the surface of a specimen

Page 19: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Scanning Electron Microscope(SEM)

Strain of Staphylococcus aureus bacteria (20,000x)

sample is coated with a very thin layer of metal

again, color has been added with a computer

Page 20: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Scanning Electron Microscope(SEM)

Page 21: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Comparing Magnificationand Resolution

Microscope Type

Magnification

Resolution

CLM 2,000 x 200 nmTEM 50,000,000 x 50 pmSEM 500,000 x 1 nm

(1 nm = 1,000 pm)nm = nanometer (1 nm =1 billionth of a meter)pm = picometer (1 pm = 1 trillionth of a meter)

Page 22: Microscopes help us see small objects typically less than 500 nm nm = nanometer = 1/1,000,000 mm size of a human egg size of a sharp pencil point

Microscope Collage

See Microscope Collage rubric.

Other Resources to use:Textbook

○ Pages 50 – 54


Nanometer MOSFET

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Size and Shape of Protein Molecules at the Nanometer …s/Protein hydrodn EM.pdf · Size and Shape of Protein Molecules at the Nanometer Level ... Size and Shape of Protein Molecules

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Electrodeposition of Single nm Size Pt Nanoparticles at a ...bard.cm.utexas.edu/resources/Bard-Reprint/968_SI.pdf · Electrodeposition of Single nm Size Pt Nanoparticles at a Tunneling

Spindle Bearing 0,6 - 10 Nm Size 2 - Core Tool Technologies

Nanometer Structures

KL-parameterization of atmospheric aerosol size distribution [email protected] University of Tartu, Institute of Physics Growth of nanometer particles

Orthomyxoviridae Influenza Virus. Properties Spherical Virion ( polymorphic) Spherical Virion ( polymorphic) 100 nm Size 100 nm Size Segmented –SS-RNA

Nanocaduceus 17 April 2005. Nano = Dwarf (Greek) Nanometer = 1 Billionth of a Meter Size of 3 Atoms…

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Environmental Technology Verification Report · PDF filecarbon (TOC), ultraviolet absorbance (UVA) at 254 nanometer (nm), iron, ... gpm) the hydrodynamic value of the contactor is

Introduction to Nanotechnology · 2011-05-14 · Nanotechnology deals with creation of materials, devices and systems in the nanometer scale (1-100 nm) through manipulating matter

Reliability in Reliability in Nanometer Technologies Nanometer … · 2017. 10. 19. · PPGEE ’08 Reliability in Reliability in Nanometer Technologies Nanometer Technologies –

Aerosol Science and Technology Transfer Functions and …authors.library.caltech.edu/22560/1/Jiang2011p12770Aerosol_Sci_T… · resolution in the nanometer size range (Rosell-Llompart

A Nanoparticle Nebulizer for Generation of Aerosolized ... · Aerosol particle size distribution Primary particle size (nm, log scale) Primary particle size (nm, linear scale) Normalized

Materials Science and Engineering · 2010. 1. 12. · Carbon buckyball ~1 nm diameter Self-assembled, Nature-inspired structure Many 10s of nm Microwo r ld 0.1 nm 1 nanometer (nm)

A Cross-Method Comparison of Sub-10 nm Nanoparticle Size

NANO LETTERS High-Speed, Sub-15 nm Feature Size

4. Nanometer-thin films

Nanometer CMOS VLSI Circuits

90 Nanometer

Nanotechnology: Applications, techniques, approaches, & the · 2019-07-16 · materials, devices and systems by manipulating shape and size at nanometer scale. At nanometer level,