A typical Fluorescence Image taken by Nikon Eclipse TE2000-U inverted microscope with a 100×/1.40 NA oil objective

Nanometer Resolution Detection

Nanometer Resolution Imaging and Computational Analysis of Axonal Transport Defects in Drosophila Models of Alzheimer’s Disease

Minhua QiuDepartment of Biomedical Engineering

Advisor: Dr. Ge Yang

System Verification

Nikon Eclipse TE2000-U inverted microscope

Fluorescent beads

NanometerResolution Piezo Stage

0 nm

8 nm

16 nmOperated by Labview

Multi-peakFitting

0 2 4 6 secs

Analysis of Axonal Transport Defects in Fly Models of AD

Brain Segmental

Nerves

Distance: 88 µm

FluorescenceImaging

APP-YFP vesicle imaged

NanometerResolutionDetection

TrajectoryRecovery

PatternRecognition

Library ofTransport

Defects