michael lalli, nimesh patel, david · pdf filecellular flow `saline bags will be used ......
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Michael Lalli, Nimesh Patel, David Pillittere
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ClientBudget TACS System DiagramObjectiveFunctional Parts of the TACS◦ Device Flow◦ Electronics◦ MaterialsConclusion
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Cell Stock Holder
Laser Diode/ PVDF Membrane apparatus
Capillary Tube
Actuating Material/Gate Mechanism
Sorted Cell Bins
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Dr. Shiva Kotha◦ UConn Professor◦ BME/ME◦ Biomechanics,
Rehabilitation Engineering & Ergonomics
◦ Bronwell 204
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BudgetAgreed on $5000Research will require several prototypesOrdering to begin immediately
First Items:Capillary TubingPVDF membranesConductive epoxy
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Project Purpose
Allow Dr. Kotha to further his researchUse different scientific methods of cell sortingDevice SizeFuture uses of the T.A.C.S.
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Previous Work in Cell SortingInvitrogen
Flow Cytometry◦ Light based ◦ Large Size◦ High Cellular flow rate
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Why is T.A.C.S Special?T.A.C.S. incorporates the thermo acoustic theory to achieve the same goals as currently used cell sorters◦ Ultrasonic waves detected by PVDF membranes◦ Actuating Material to direct the flow of the sorted
cells using a voltage◦ Smaller size allowing for multiple uses
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Overview of SystemNew cell enters
capillary tube
Cell type determination
and gate actuation
Cell enervation by
the laser diode
Cell expansion and contraction releases
ultrasonic wave
Voltage response by PVDF
film
Cell flows through gate a and into bin a
Unsorted cells
Bin A
Cell flows through gate b and into bin b
Bin B
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Cellular FlowSaline bags will be used to hold the cell stockCapillary tubes will be attached to the ends of the bagsSorted bins will allow for maximum flow of the stock if only one type of cell is present
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Flow DevicesHydrodynamic focusing will potentially be used to control the rate of flow
This device allows for single-file cellular flow
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Capillary Tubing
Borosilicate glass-Boron infused silica
Low thermal expansion
High transmittance of light
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Electronic SystemConsists of:◦ Laser Diode◦ PVDF Sensors◦ Analog Filters◦ Operational Amplifiers◦ Data Acquisition (National Instruments)◦ Software Program◦ Actuating Gates
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Laser DiodeA 670 nm Laser DiodeInexpensiveTrypan blue dye absorbs all wavelengths of light above 500 nm
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PVDF Film - Polyvinylidene Fluoride
Piezoelectric when poled◦ Generates a voltage when the shape
changes and vice versaCommonly used as ultrasonic sensorsDifferent thicknesses respond to different frequency rangesChallenges◦ Cannot be soldered◦ Must adhere to the micro channel
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Analog FiltersAnalog filters will only be used if necessaryLikely filters to be used◦ Notch filter with cutoff frequency of 60Hz◦ High pass filter to remove interference from
vibrations in the surrounding environment especially sound.
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Operational AmplifierStrengthens signalPrevents damage to the data acquisition device
Connect the analog output to the actuating gate
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Data Acquisition - NI USB-6009
8 Analog inputs (+- 10v)Sampling Frequency of 48Khz2 Analog outputs (0-5v)Compatible with LabVIEWRelatively inexpensive ($279)
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Software SystemConsists of:◦ Digital Filtering◦ Feature Extraction
Quantitatively identify important information within the signal to help in differentiating between cell types
◦ ClassificationArtificial Neural NetworkSimplifies ClassificationSupervised Machine Learning (Back-propagation algorithm)
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Actuating Gate
Charged Zeolite crystal
Absorb ions upon electrical stimulation
Increases in volume
Selectively blocks and changes flow
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QuestionsAre there any?
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In Case You Ask about Artificial Neural Networks