Mechanistic Insights into Ferredoxin-NADP(H)-ReductaseCatalysis Involving the Conserved Glutamate in the Active

Site

Veronica I. Dumit,1,2,+ Timm Essigke,1,+ Nestor Cortez,2

and G. Matthias Ullmann1,∗

1 Structural Biology / Bioinformatics, University of Bayreuth, Universitatsstr. 30,

BGI, 95447 Bayreuth, Germany

2 Instituto de Biologıa Molecular y Celular de Rosario, Universidad Nacional de

Rosario, Suipacha 531, S2002LRK Rosario, Argentina

∗ to whom correspondence should be addressed; Email: Matthias.Ullmann@uni-

bayreuth.de; Fax: +49-921-55-3544

+ authors contributed equally

Supporting Information

1

Table 1: Partial Charges of the flavin isoalloxazine ring.

Atom FAD FADH. FADH2 FAD.−

N1 -0.744883 -0.792492 -0.752827 -0.778810HN1 0.000000 0.000000 0.459134 0.000000C2 0.979264 0.996910 0.967921 0.980265O2 -0.701395 -0.738142 -0.719930 -0.807856N3 -0.729075 -0.741433 -0.729028 -0.761840H3 0.413039 0.408825 0.413084 0.402695C4 0.723433 0.748511 0.744274 0.724561O4 -0.635995 -0.678005 -0.683674 -0.743137C4X 0.214096 -0.182070 -0.234883 0.077900N5 -0.601790 -0.272552 -0.419799 -0.775444HN5 0.000000 0.352647 0.332867 0.000000C5X 0.495945 0.210617 0.239268 0.542325C6 -0.352594 -0.332772 -0.398402 -0.395594H6 0.176242 0.187306 0.187098 0.141026C7 0.127757 0.114925 0.110814 0.097311C8 0.069069 0.073721 0.022091 0.000077C9 -0.249185 -0.290764 -0.310631 -0.271688H9 0.194324 0.191728 0.192713 0.180241C9A -0.046423 0.108567 0.090894 -0.105126N10 -0.011589 -0.155388 -0.221859 0.017271C10 0.351818 0.513896 0.467344 0.288310C7M -0.149784 -0.172592 -0.142909 -0.099306H7M1 0.059883 0.066580 0.051825 0.035933H7M2 0.065753 0.064723 0.048772 0.046150H7M3 0.059979 0.066603 0.052026 0.036059C8M -0.136753 -0.166658 -0.128109 -0.108192H8M1 0.071014 0.068870 0.047390 0.048917H8M2 0.071414 0.069429 0.048386 0.048696H8M3 0.051215 0.058372 0.042280 0.035073C1’ 0.230208 0.215778 0.214766 0.144412H11’ 0.002521 0.002430 0.004553 -0.000113H12’ 0.002521 0.002430 0.004553 -0.000113

2

Table 2: Partial Charges of the iron-sulfur cluster.

Atom ox redFE1 0.820000 0.963000FE2 0.797000 0.952000S1 -0.637000 -0.864000S2 -0.590000 -0.8520001CB -0.048000 -0.0360001HB1 0.041000 0.0170001HB2 0.013000 -0.0590001SG -0.593000 -0.7520002CB -0.003000 0.0160002HB1 0.037000 0.0190002HB2 0.007000 -0.0850002SG -0.661000 -0.8010003CB -0.052000 -0.1490003HB1 0.034000 0.0590003HB2 0.041000 0.0230003SG -0.636000 -0.7250004CB 0.058000 -0.0380004HB1 0.040000 0.0940004HB2 -0.039000 -0.0570004SG -0.629000 -0.725000

3

Table 3: Protonation ∆GTR,7 and energy contributions, ∆∆GBorn, ∆∆Gback, ∆GinterTR,7 of

Glu312 in different steps of the reaction cycle, calculated at pH 7.0 (kcal/mol). In case ofthe flavin the three forms are FAD (ox), FADH� (se) and FADH2 (red), in case of NADP(H) thetwo forms are NADP+ (ox) and NADPH (red). Structures were modeled based on PDB entry1gaw for the intermediates without Fd bound and based on PDB entry 1gaq for intermediateswith Fd. The major difference in the FNR structure between the two PDB entries is a differentrotamer of Glu312. Therefore, the calculations without Fd were also done with PDB entry 1gaqwith Fd deleted (intermediates marked by *).

FNR complex IntermediateFlavin NADP(H) Fd ∆GTR,7 ∆∆GBorn ∆∆Gback ∆Ginter

TR,7 Figure 4ox 2.8 -2.2 0.7 0.6 Ise 3.1 -2.2 1.0 0.6red 2.5 -2.2 0.4 0.6ox ox 4.9 -2.2 2.8 0.7 IIse ox 5.3 -2.2 3.2 0.7 Vred ox 4.7 -2.2 2.5 0.7 VIIIbox red 2.8 -2.2 0.7 0.7 IXse red 3.2 -2.2 1.1 0.7

FNR

with

outF

dbo

und

red red 2.6 -2.2 0.5 0.7ox -1.4 -7.0 1.1 0.8 I*se -0.6 -7.0 1.9 0.8red -1.6 -7.0 0.9 0.8ox ox 3.3 -7.2 6.0 0.8 II*se ox 4.1 -7.2 6.8 0.8 V*red ox 3.1 -7.2 5.8 0.9 VIIIb*ox red -0.3 -7.2 2.3 0.9 IX*se red 0.5 -7.2 3.2 0.9red red -0.5 -7.2 2.2 0.9ox ox ox -0.3 -11.2 5.9 1.3se ox ox 1.0 -11.2 7.1 1.4 IVred ox ox -1.0 -11.2 5.2 1.3 VIIox red ox -5.9 -11.2 0.2 1.4 VIIIase red ox -4.8 -11.2 1.3 1.4red red ox -6.6 -11.2 -0.5 1.4ox ox red -2.5 -11.2 3.6 1.4 IIIse ox red -1.3 -11.2 4.7 1.4 VIred ox red -3.2 -11.2 2.9 1.4ox red red -8.1 -11.2 -2.1 1.6se red red -6.9 -11.2 -1.0 1.6

FNR

with

Fdbo

und

red red red -8.7 -11.2 -2.8 1.6ox ox -6.6 -11.1 -0.5 1.3se ox -5.4 -11.1 0.7 1.3red ox -7.2 -11.1 -1.1 1.3ox red -8.7 -11.1 -2.7 1.4se red -7.5 -11.1 -1.6 1.4red red -9.3 -11.1 -3.3 1.5

4

Alignment of Bacterial FNRs

Oceanicaulis_alexandrii ..............................Maricaulis_maris ..............................Mesorhizobium_loti ..............................Mesorhizobium_sp. ..............................Aurantimonas_sp. ..............................Fulvimarina_pelagi ..............................Bartonella_quintana ..............................Bartonella_henselae ..............................Bartonella_tribocorum ..............................Bartonella_bacilliformis ..............................Hoeflea_phototrophica ..............................Sinorhizobium_meliloti ..............................Sinorhizobium_medicae ..............................Agrobacterium_tumefaciens ..............................Rhizobium_leguminosarum MEWNAFNSSPICNICLASDSSSDETGSATARhizobium_etli ..............................Stappia_aggregata ..............................Hyphomonas_neptunium ..............................Rhodobacter_sphaeroides ..............................Paracoccus_denitrificans ..............................Rhodobacter_capsulatus ..............................Jannaschia_sp. .............................MLoktanella_vestfoldensis ........................MGHDEHDinoroseobacter_shibae ..........................MNKPPhaeobacter_gallaeciensis ...........................MDPSilicibacter_sp. .............................MSagittula_stellata ...........................MDTOceanicola_batsensis ..........................MARDRoseovarius_sp. ............................MNRoseobacter_denitrificans ..............................Roseobacter_litoralis ..............................Roseovarius_nubinhibens ..............................Silicibacter_pomeroyi ..............................Sulfitobacter_sp. ..............................Oceanibulbus_indolifex ..............................Rhodobacterales_bacterium ..............................Oceanicola_granulosus ..............................Burkholderia_dolosa ..............................Burkholderia_multivorans ..............................Burkholderia_cenocepacia ..............................Burkholderia_ambifaria ..............................Burkholderia_vietnamiensis ..............................Burkholderia_ubonensis ..............................Burkholderia_thailandensis ..............................Burkholderia_pseudomallei ..............................Burkholderia_oklahomensis ..............................Hahella_chejuensis ..............................Reinekea_sp. ..............................Pseudomonas_putida ..............................Pseudomonas_entomophila ..............................Pseudomonas_fluorescens ..............................Pseudomonas_mendocina ..............................Ralstonia_eutropha .......MHIPEIPRWRCRLRLRRPQACVKCupriavidus_taiwanensis ..............................Methylobacterium_radiotolerans ..............................Methylobacterium_extorquens ..............................Methylobacterium_nodulans ..............................Xanthobacter_autotrophicus ..............................Brucella_melitensis ..............................Brucella_ovis ..............................Brucella_suis ..............................Ochrobactrum_anthropi ..............................Psychrobacter_arcticus ..............................Psychrobacter_cryohalolentis ..............................Psychrobacter_sp. ..............................Caulobacter_crescentus ..............................consensus

5

10.Oceanicaulis_alexandrii MAM...................GAFTEETVMaricaulis_maris MTMTATAP....AKKNAVSAKPGPFTVETVMesorhizobium_loti MNTTSAFN..TAGARPLQFPIPANVYAETVMesorhizobium_sp. MSTAALQN..VEAADKLEFPIPAGVYAERVAurantimonas_sp. MSSLAQQ...PEHSPEPAFPIPAGVFAETVFulvimarina_pelagi MSSVAEK...TAET..ESFPIPANVFAETVBartonella_quintana MNTPATHVQSNINSVSSSFPIPENVFALTVBartonella_henselae MNTSASNVQSNISSVASDFPIPENVFALTVBartonella_tribocorum MNTSATNVPSNISSVA.DFPIPANVFALTVBartonella_bacilliformis MSTSIPNVKADISSTASNFPIPENVFTLTVHoeflea_phototrophica MNQIVR.....PTSNAGSYEIPAGVFAETVSinorhizobium_meliloti MNAPAK.......KEDFAVQAPAGVFAETVSinorhizobium_medicae MNAPAK.......KEDFAVQAPAGVFVETVAgrobacterium_tumefaciens MNAPAK.......TEDFAIKIPDGVYAETVRhizobium_leguminosarum MNAPAK.......TEDFASSIPAGVYAETVRhizobium_etli MNAPAK.......TEDFASSIPAGVYAETVStappia_aggregata MNVLAK.......PADLEAAAPAGAFVEEVHyphomonas_neptunium MKVN.................PNGPTEETVRhodobacter_sphaeroides ......MNQ.......NAAIVKTLPDAQTVParacoccus_denitrificans MTLDLSVDI.......AAKPAKTLPDAQTVRhodobacter_capsulatus ..TTVNETT.......PIAPAKVLPDAQTVJannaschia_sp. MTEQTPVTQTD...AAPVKATPTLPDAQTVLoktanella_vestfoldensis MTEQSPVNH......ASAKIVPQLPDMQTVDinoroseobacter_shibae TDVMSTVTD......ATPIKVPTLPDAQTVPhaeobacter_gallaeciensis MNEMTPVTEAATDPK.PAKAVPALPDAQTVSilicibacter_sp. MNEITPVSEPVTEAAAPAKAKPALPDAQTVSagittula_stellata MTEMTPVTDATAAATAPAKK..AVPDAQTVOceanicola_batsensis DMTEATVTE......QAIPKVPTLPDAQTVRoseovarius_sp. EMTAVTQAT......AKAPKTLAIPDAQIVRoseobacter_denitrificans MTEHAPVNK......EAATKVPTLPDAQTVRoseobacter_litoralis MTEQATVNK......ETATKVPTLPDAQTVRoseovarius_nubinhibens MNEIADVTD.....ASPIKAKPALPDAQKVSilicibacter_pomeroyi MTEMRPVTE......AAAIKAPTLPDSQTVSulfitobacter_sp. MTEQNVVTQ......TDAKPVPTLPDAQTVOceanibulbus_indolifex MTEQTKVNQ......TAAKPVPTLPDAQSVRhodobacterales_bacterium MTEQNPVNT......ENAKPVKALPDAQIVOceanicola_granulosus MTEQSTVTQ......ADARPVKALPDAQIVBurkholderia_dolosa ...................MSK..FDTATVBurkholderia_multivorans ...................MSK..YDTAIVBurkholderia_cenocepacia ...................MSK..YDTATVBurkholderia_ambifaria ...................MSK..YDTATVBurkholderia_vietnamiensis ...................MSK..YDTATVBurkholderia_ubonensis ...................MSK..YDTATVBurkholderia_thailandensis ...................MSK..FDTATVBurkholderia_pseudomallei ...................MSK..FDTATVBurkholderia_oklahomensis ...................MSK..FDTATVHahella_chejuensis ...................MSG..MRKETVReinekea_sp. ...................MANT.LRKETVPseudomonas_putida ...................MSN..MNHERVPseudomonas_entomophila ...................MSN..MNHERVPseudomonas_fluorescens ...................MSN..MNHERVPseudomonas_mendocina ...MPRNRVFRRPILEEVIMSN..MNVERVRalstonia_eutropha ILPAVFGAASCRCIPR.FAMSN..LNQQSICupriavidus_taiwanensis ..........MRCIPR.LAMSN..LNQQSIMethylobacterium_radiotolerans ...................MSK..FHEARVMethylobacterium_extorquens ...................MSK..YNEERVMethylobacterium_nodulans ...................MSN..FNEERVXanthobacter_autotrophicus MWPSPFRARSGACRDRILKMSN..FNEETVBrucella_melitensis ..................MSSN..FNQETVBrucella_ovis ..................MSSN..FNQETVBrucella_suis ..................MSSN..FNQETVOchrobactrum_anthropi ..................MSSN..FNQETVPsychrobacter_arcticus ...................MSK..LRTETVPsychrobacter_cryohalolentis ...................MSK..LRTETVPsychrobacter_sp. ...................MSK..FHTETVCaulobacter_crescentus ...................MKEAPYFVEKVconsensus

6

20. 30. 40.Oceanicaulis_alexandrii IAVRHYTDRLFSFRVTRPQSFRFRSGEFVMMaricaulis_maris LSVTHYTDRLFHFRITRPDAFRFRSGEFIMMesorhizobium_loti VSVKHYTDRLFSFRITRPQSLRFRSGEFVMMesorhizobium_sp. VAVKHYTDRLFSFRITRPQTFRFRSGEFVMAurantimonas_sp. TKVTHYTDSLFAFRITRPQSFRFRSGEFVMFulvimarina_pelagi TSVTHYTDRLFAFRMTRPQSFRFRSGEFVMBartonella_quintana QEVYHYTDHLFKFRLNRPESFRFRSGEFVMBartonella_henselae QEVYHYTDRLFKFRLNRPESFRFRSGEFVMBartonella_tribocorum QEVCHYTDRLFKFRLNRPESFRFRSGEFVMBartonella_bacilliformis QEVCHYTDRLFKFRLNRPNNFRFRSGEFIMHoeflea_phototrophica TSVQHYTDRLFKIRITRPASFRFRSGEFVMSinorhizobium_meliloti TSVEHYTDRLFRFRMTRPQEFRFRSGEFAMSinorhizobium_medicae TSVEHYTDRLFRFRMTRPKEFRFRSGEFAMAgrobacterium_tumefaciens LSVEHYTDHLFRFRMTRPAGFRFRSGEFAMRhizobium_leguminosarum LSVTHYTDRLFRFTMTRPQGFRFRSGEFAMRhizobium_etli LAVTHYTDRLFRFTMTRPQGFRFRSGEFAMStappia_aggregata KFVQHYTDRLFRFRMTRPASFRFRSGEFVMHyphomonas_neptunium LSVEHYTDRLFRFRLTRPQSFRFRTGEFVMRhodobacter_sphaeroides TSVQHWTDRLFSFRVTRPQSLRFRSGEFVMParacoccus_denitrificans TSVRHWTDRLFSFRVTRPASLRFRSGEFVMRhodobacter_capsulatus TSVRHWTDTLFSFRVTRPQTLRFRSGEFVMJannaschia_sp. TEVKHYTDRLFSFRCTRPASLRFRSGEFVMLoktanella_vestfoldensis TEVKHYTDRLFSFRVTRPASLRFRSGEFVMDinoroseobacter_shibae LSVKHWTDRLFSFRVTRPQTLRFRSGEFVMPhaeobacter_gallaeciensis TEVKHWTDRLFSFKVTRPASLRFRSGEFVMSilicibacter_sp. TQVKHWTDRLFSFRCTRPASLRFRSGEFVMSagittula_stellata TEVRHWTDRLFSFRCTRPASLRFRSGEFVMOceanicola_batsensis THVKHWTDRLFSFRCTRPASLRFRSGEFVMRoseovarius_sp. TQVTHWTDRLFSFRVTRPASLRFRSGEFVMRoseobacter_denitrificans TAVQHYTDRLFSFRVTRPASLRFRSGEFVMRoseobacter_litoralis TAVQHYTDRLFSFRVTRPASLRFRSGEFVMRoseovarius_nubinhibens TEVTHWTDRLFSFRVTRPASLRFRSGEFVMSilicibacter_pomeroyi TQVKHWTDRLFSFRVTRPASLRFRSGEFVMSulfitobacter_sp. TSVKHWTDRLFSFRVTRPASLRFRSGEFVMOceanibulbus_indolifex TSVTHWTDQLFSFRVSRPASLRFRSGEFVMRhodobacterales_bacterium TSVTHWTDTLFSFRVTRPKSLRFRSGEFVMOceanicola_granulosus TEVRHYTDRLFSFRVSRPASLRFRSGEFVMBurkholderia_dolosa QSVHHWTDTLFSFTCTREPSLRFNNGEFTMBurkholderia_multivorans QSVHHWTDTLFSFTCTREPSLRFNNGEFTMBurkholderia_cenocepacia QSVHHWTDTLFSFTCTREASLRFNNGEFTMBurkholderia_ambifaria QSVHHWTDTLFSFTCTREPSLRFNNGEFTMBurkholderia_vietnamiensis QSVHHWTDTLFSFTCSREPSLRFNNGEFTMBurkholderia_ubonensis QSVHHWTDTLFSFTCTREPSLRFNNGEFTMBurkholderia_thailandensis LSVHHWTDTLFSFTCTRDQGLRFNNGEFTMBurkholderia_pseudomallei LSVHHWTDTLFSFTCTRDQALRFNNGEFTMBurkholderia_oklahomensis LSVHHWTDTLFSFTCTRDQALRFNNGEFTMHahella_chejuensis TSVRHWNDTLFSFTTSRDPSFRFKNGHFIMReinekea_sp. TEVHHWNESLFSFKTTRDMGFRFKNGHFVMPseudomonas_putida LSVHHWNDTLFSFKCTRDPGLRFENGQFVMPseudomonas_entomophila LSVHHWNDTLFSFKCTRDPGLRFENGQFVMPseudomonas_fluorescens LSVHHWNDTLFSFKCTRDPGLRFENGQFVMPseudomonas_mendocina LSVHHWNDTLFSFKCTRDPGLRFENGQFVMRalstonia_eutropha LSVHHWTDTLFSFTCTRDPGFRFENGQFAMCupriavidus_taiwanensis LSVHHWTDTLFSFTCTRDPGFRFENGQFAMMethylobacterium_radiotolerans LSVHHWTDTLFSFRTTRDPAFRFRNGEFTMMethylobacterium_extorquens LSVHHWTDTLFSFRTTRDPSFRFRNGEFTMMethylobacterium_nodulans LSVHHWTDTLFSFRTTRDPSFRFRNGEFTMXanthobacter_autotrophicus TSVHHWTDTLFSFTCTRDPGLRFLNGQFTMBrucella_melitensis TDIHHWTDTLFSFRTTRDPGFRFQSGQFIMBrucella_ovis TDIHHWTDTLFSFRTTRDPGFRFQSGQFIMBrucella_suis TDIHHWTDTLFSFRTTRDPGFRFQSGQFIMOchrobactrum_anthropi TDIHHWTDTLFSFRTTRDPGFRFQSGQFIMPsychrobacter_arcticus TEVHHWNDALFSIKTTRDDGLRFRNGEFAMPsychrobacter_cryohalolentis TEVHHWNDALFSIKTTRDDGLRFRNGEFAMPsychrobacter_sp. TYVHHWNDSLFTIKTTRDAGLRFRNGEFAMCaulobacter_crescentus LWVKHWTDRLFSFAITRPASFRFRSGEFVMconsensus * ** * ** * * *

7

50. 60.Oceanicaulis_alexandrii IGL.MVDG......KPLLRAYSIASPSWDEMaricaulis_maris IGLPKEDG......KPLLRAYSLASPFWDEMesorhizobium_loti IGLPNAE.......KPVFRAYSVASPAWDEMesorhizobium_sp. IGLPNAE.......KPVFRAYSIASPAWDEAurantimonas_sp. IGLPNAA.......KPVYRAYSIASPAWDEFulvimarina_pelagi IGLPNAE.......KPVYRAYSIASPAWDEBartonella_quintana IGLPNAE.......KPIYRAYSIASPFWDEBartonella_henselae IGLPNAE.......KPIYRAYSIASPFWDEBartonella_tribocorum IGLPNAE.......KPIYRAYSIASPFWDEBartonella_bacilliformis IGLPNAE.......KPVYRAYSIASPFWDEHoeflea_phototrophica IGLPNAE.......KPVFRAYSIASPSWDESinorhizobium_meliloti IGLMVGD.......KPVYRAYSIASPAWDESinorhizobium_medicae IGLMVGD.......KPIYRAYSIASPAWDEAgrobacterium_tumefaciens IGLMVGE.......KPIYRAYSIASPAWDERhizobium_leguminosarum IGLMVEG.......KPVFRAYSIASPAWAERhizobium_etli IGLMVEG.......KPVFRAYSIASPAWAEStappia_aggregata IGLMIDS.......KPLYRAYSIASPAWDEHyphomonas_neptunium IGLPKEDG......KPLLRAYSIASPAWDERhodobacter_sphaeroides IGLLDE......RGKPIMRAYSIASPNWDEParacoccus_denitrificans IGLPDD......NGKPILRAYSIASPNWDERhodobacter_capsulatus IGLLDD......NGKPIMRAYSIASPAWDEJannaschia_sp. IGLMGEPHPETGKQKPLLRAYSIASPSWDELoktanella_vestfoldensis IGLMGDEDPETGKRKPIMRAYSIASPSWDEDinoroseobacter_shibae IGLMGDPDPKTGKQKPLLRAYSIASPSWDEPhaeobacter_gallaeciensis IGLMNDPDPKTGKVKPLLRAYSIASPSWDESilicibacter_sp. IGLMGDPDPKTGKQKPLLRAYSIASPSWDESagittula_stellata IGLMQT.DPKSGKEKPLLRAYSIASPSWDEOceanicola_batsensis IGLMG....DTG..RPLLRAYSIASPSWDERoseovarius_sp. IGLMQT.DEKTGKEKPLLRAYSIASPSWDDRoseobacter_denitrificans IGLMGDPDPKTGKQKPLLRAYSIASPSWDDRoseobacter_litoralis IGLMGDPDPKTGKQKPLMRAYSIASPSWDDRoseovarius_nubinhibens IGLMGDPHPETGKQKPLLRAYSIASPSWDESilicibacter_pomeroyi IGLLGD......NGKPLLRAYSIASPAWDESulfitobacter_sp. IGLMGDPHPETGKQKPLLRAYSIASPSWDEOceanibulbus_indolifex IGLMGDPHPETGKQKPLLRAYSIASPAWDDRhodobacterales_bacterium IGLLKD......DGRPLLRAYSIASPSWDEOceanicola_granulosus IGLMGD......NGKPLLRAYSIASPSWDDBurkholderia_dolosa VGL......EVD.GKPLTRAYSIVSPNYEEBurkholderia_multivorans VGL......EVD.GKPLARAYSIVSPNYEEBurkholderia_cenocepacia VGL......EVD.GKPLARAYSIVSPNYEEBurkholderia_ambifaria VGL......EVD.GKPLARAYSIVSPNYEEBurkholderia_vietnamiensis VGL......EVD.GKPLARAYSIVSPNYEDBurkholderia_ubonensis VGL......EVD.GKPLARAYSIVSPNYEEBurkholderia_thailandensis VGL......EVD.GKPLTRAYSIVSPNYEEBurkholderia_pseudomallei VGL......EVD.GKPLTRAYSIVSPNYEEBurkholderia_oklahomensis VGL......EVD.GKPLTRAYSIVSPNYEEHahella_chejuensis IGL......EQDSGRPLMRAYSIASANYEEReinekea_sp. IGL......EKDDGRPLMRAYSIASANYEEPseudomonas_putida IGL......QQDNGRPLMRAYSIASPNWEEPseudomonas_entomophila IGL......QQDSGRPLMRAYSIASPNWEEPseudomonas_fluorescens IGL......QQPNGRPLMRAYSIASPNWEEPseudomonas_mendocina IGL......QQDNGRPLMRAYSIASPNWEERalstonia_eutropha VGL......EVN.GRPLLRAYSIASANYEECupriavidus_taiwanensis VGL......EVN.GRPLLRAYSIASANYEEMethylobacterium_radiotolerans IGL......EVE.GRPLLRAYSVVSANYEDMethylobacterium_extorquens IGI......EVE.GRPLLRAYSVVSANYEEMethylobacterium_nodulans IGL......KSD.GKPLLRAYSVVSANYEDXanthobacter_autotrophicus IGL......KVD.GKPLLRAYSMASANYEPBrucella_melitensis MGL......EVN.GKPLTRAYSIASSLYEDBrucella_ovis MGL......EVN.GKPLTRAYSIASSLYEDBrucella_suis MGL......EVN.GKPLTRAYSIASSLYEDOchrobactrum_anthropi MGL......EVN.GKPLTRAYSIASSLYEDPsychrobacter_arcticus IGL......VVD.GKPLLRAYSIASPNYEEPsychrobacter_cryohalolentis IGL......VVD.GKPLLRAYSIASPNYEEPsychrobacter_sp. IGI......MVD.GKPLMRAYSIASPNYEECaulobacter_crescentus IGLPPRE..ELGEKKPILRAYSIGSPSFAEconsensus * * **** *

8

70. 80. 90.Oceanicaulis_alexandrii ELDFYSIKVPDGPLTSQLQHIEPGDTILMGMaricaulis_maris ALDFYSIKVPDGPLTSRLQHIKQGDEVLLGMesorhizobium_loti ELEFFSIKVPDGPLTSELQKIQVGDTVIMRMesorhizobium_sp. ELEFFSIKVPDGPLTQHLQKITPGDIVLMRAurantimonas_sp. ELEFFSIKVANGPLTEHLQKIQVGDTVLMRFulvimarina_pelagi EIEFFSIKVPGGPLTEHLQKIQEGDTVLMRBartonella_quintana QLEFFSIKVPGGSLTEHLQKIKIGDTVLMRBartonella_henselae QLEFFSIKVPGGPLTEHLQKIKIGDTVLMRBartonella_tribocorum QLEFFSIKVPGGPLTEHLQKIKIGDTVLMRBartonella_bacilliformis QLEFFSIKVPGGPLTEHLQKIKIGDTVLMRHoeflea_phototrophica EIEFYSIKVPGGPLTEHLQKIVPGDTLLMRSinorhizobium_meliloti ELEFFSIKVPDGPLTSHLQGIKPGDQVLMRSinorhizobium_medicae ELEFFSIKVPDGPLTSHLQGIKPGDQVLMRAgrobacterium_tumefaciens ELEFFSIKVPDGPLTSRLQAIKPGDTVLMRRhizobium_leguminosarum ELEFFSIKVPDGPLTSHLQAIKPGDQVLMRRhizobium_etli ELEFFSIKVPDGPLTSHLQAIKPGDQVLMRStappia_aggregata ELEFFSIKVPDGPLTSHLQKIQPGDAVLMKHyphomonas_neptunium ELEFYSIKVPDGPLTSRLQKIQPGDKVLLGRhodobacter_sphaeroides ELEFYSIKVPDGPLTSRLQHIQPGDQIILRParacoccus_denitrificans ELEFYSIKVPDGPLTSRLQNIQPGDQIILRRhodobacter_capsulatus ELEFYSIKVPDGPLTSRLQHIKVGEQIILRJannaschia_sp. ELEFYSIKVPDGPLTSRLQHIQPGEQIILRLoktanella_vestfoldensis ELEFYSIKVQDGPLTSKLQHIQPGDGLILRDinoroseobacter_shibae ELEFYSIKVPDGPLTSRLQHIQPGEQIILRPhaeobacter_gallaeciensis EMEFYSIKVQDGPLTSRLQHIKVGDEIILRSilicibacter_sp. EMEFYSIKVQDGPLTSKLQHIKVGDEIILRSagittula_stellata ELEFYSIKVQDGPLTSRLQHIQPGDEIVLROceanicola_batsensis ELEFYSIKVQDGPLTSRLQHLAVGDEIILRRoseovarius_sp. ELEFYSIKVQDGPLTSRLQHIEVGDEIILRRoseobacter_denitrificans ELEFYSIKVPDGPLTSRLQHIQVGDEIILRRoseobacter_litoralis ELEFYSIKVQDGPLTSRLQHIQVGDEIILRRoseovarius_nubinhibens ELEFYSIKVQDGPLTSKLQHIQPGDEIILRSilicibacter_pomeroyi ELEFYSIKVQDGPLTSKLQHIQPGDEIILRSulfitobacter_sp. ELEFYSIKVQDGPLTSKLQHIQPGDQIILROceanibulbus_indolifex ELEFYSIKVQDGPLTSKLQHIQPGDQIILRRhodobacterales_bacterium ELEFYSIKVQDGPLTSRLQHLKVGDELILROceanicola_granulosus ELEFYSIKVPDGPLTSKLQHIQPGDQIILRBurkholderia_dolosa HLEFFSIKVQDGPLTSRLQHLKVGDKVLIGBurkholderia_multivorans HLEFFSIKVQDGPLTSRLQHLKVGDTVLIGBurkholderia_cenocepacia HLEFFSIKVQDGPLTSRLQHLKVGDTVLIGBurkholderia_ambifaria HLEFFSIKVQNGPLTSRLQHLKVGDSVLIGBurkholderia_vietnamiensis HLEFFSIKVQDGPLTSRLQHLKVGDTVLIGBurkholderia_ubonensis HLEFFSIKVQNGPLTSRLQHLKVGDTVLIGBurkholderia_thailandensis HLEFFSIKVQNGPLTSRLQHLKIGDPVLIGBurkholderia_pseudomallei HLEFFSIKVQNGPLTSRLQHLKVGDPVLIGBurkholderia_oklahomensis HLEFFSIKVQNGPLTSRLQHLKVGDAVLIGHahella_chejuensis ELEFFSIKVPDGPLTSRLQKISVGDEIIMSReinekea_sp. ELEFFSIKVPDGPLTSRLQKIQVGDEIVVGPseudomonas_putida HLEFFSIKVPDGPLTSQLQHLKEGDEIIISPseudomonas_entomophila HLEFFSIKVPDGPLTSQLQHLKEGDEIIISPseudomonas_fluorescens HLEFFSIKVPDGPLTSQLQHLKEGDEIIISPseudomonas_mendocina HLEFFSIKVPDGPLTSQLQHLKEGDEIIISRalstonia_eutropha TLEFFSIKVPDGPLTSRLQHLREGDQIFVGCupriavidus_taiwanensis TLEFFSIKVPDGPLTSRLQHLREGDQIYVGMethylobacterium_radiotolerans ELEFFSIKVPDGPLTSKLQHLKVGDPIIVGMethylobacterium_extorquens ELEFFSIKVPNGPLTSKLQHLKVGDPIMIGMethylobacterium_nodulans ELEFFSIKVPNGPLTSKLQHLKVGDPIIVSXanthobacter_autotrophicus DLQFFSIKVQNGPLTSRLQHLKVGDKLLVGBrucella_melitensis GLEFFSIKVPNGPLTSKLQHLKKGDQIIVSBrucella_ovis GLEFFSIKVPNGPLTSKLQHLKKGDQIIVSBrucella_suis GLEFFSIKVPNGPLTSKLQHLKKGDQIIVSOchrobactrum_anthropi GLEFFSIKVPNGPLTSKLQHLKVGDQIILSPsychrobacter_arcticus HLEFFSIKVQDGPLTSRLQHIKVGDELLVSPsychrobacter_cryohalolentis HLEFFSIKVQDGPLTSRLQHIKVGDELLVSPsychrobacter_sp. HLEFFSIKVQDGPLTSRLQHIKVGDELIISCaulobacter_crescentus ELEFFSIKVPDGPLTSRLQLIQEGDEILLGconsensus * **** * ** ** *

9

100. 110. 120.Oceanicaulis_alexandrii KKSVGTLVLDALEPGKRLYLLSTGTGIAPFMaricaulis_maris RKPTGTLVLDALKPGKRLYMISTGTGIAPFMesorhizobium_loti QKSTGTLVVDALTPAKRLFMISTGTGIAPFMesorhizobium_sp. QKSTGTLVNDALTPAKRLYMISTGTGIAPFAurantimonas_sp. KKPTGTLVHDALKPGKRLFLFSTGTGIAPFFulvimarina_pelagi KKPTGTLVHDALIPGKRLFLFSTGTGIAPFBartonella_quintana KKSTGTLVLDALIPGKRLYLLSTGTGVAPFBartonella_henselae KKSTGTLVLDALIPGKRLYLLSTGTGVAPFBartonella_tribocorum KKSTGTLVLDALIPGKRLYLLSTGTGVAPFBartonella_bacilliformis KKSTGTLVLDALIPGKRLYLLSTGTGVAPFHoeflea_phototrophica KKPTGTLVLDALVPGKRLYMFSTGTGIAPFSinorhizobium_meliloti KKPTGTLVLDALVPGRRLYMFSTGTGIAPFSinorhizobium_medicae KKPTGTLVLDALVPGRRLYMFSTGTGIAPFAgrobacterium_tumefaciens KKPTGTLVLDALTPGRRLYMFSTGTGIAPFRhizobium_leguminosarum KKPTGTLVLDALTPGRRLYMFSTGTGIAPFRhizobium_etli KKPTGTLVLDALTPGRRLYMFSTGTGVAPFStappia_aggregata KKPTGTLVNDALIPGKRVYMFSTGTGIAPFHyphomonas_neptunium RKPTGTLVLDALTPGKRLYMFSTGTGFAPFRhodobacter_sphaeroides PKPVGTLVLDALLPGKRIWFLATGTGIAPFParacoccus_denitrificans PKPVGTLVLDALLPGKRMWFLATGTGIAPFRhodobacter_capsulatus PKPVGTLVIDALLPGKRLWFLATGTGIAPFJannaschia_sp. PKPVGTLVHDALLPGKRLWFFATGTGFAPFLoktanella_vestfoldensis PKPVGTLVHDALLPGKRLWFFATGTGFAPFDinoroseobacter_shibae PKPVGTLVHDALLPGKRIWFFATGTGFAPFPhaeobacter_gallaeciensis PKPVGTLVHDALIPGKRIWFFATGTGFAPFSilicibacter_sp. PKPVGTLVHDALLPGKRIWFFATGTGFAPFSagittula_stellata PKPVGTLVHDALLPGKRIWFFATGTGFAPFOceanicola_batsensis PKPVGTLVHDALLPGKRIWFFATGTGFAPFRoseovarius_sp. PKPVGTLVHDALLPGKRIWFFATGTGFAPFRoseobacter_denitrificans PKPVGTLVHDALLPGKRIWFFATGTGFAPFRoseobacter_litoralis PKPVGTLVHDALLPGKRIWFFATGTGFAPFRoseovarius_nubinhibens PKPVGTLVHDALLPGKRLWFFATGTGFAPFSilicibacter_pomeroyi PKPVGTLVHDALLPGKRIWFFATGTGFAPFSulfitobacter_sp. PKPVGTLVHDALLPGKRLWLFATGTGFAPFOceanibulbus_indolifex PKPVGTLVHDALLPGNRLWMFATGTGFAPFRhodobacterales_bacterium PKPVGTLVHDALTPGKRLWFFATGTGFAPFOceanicola_granulosus PKPVGTLVHDALLPGKRLYFFSTGTGFAPFBurkholderia_dolosa KKPTGTLVVDNLLPGKTLWLLSTGTGLAPFBurkholderia_multivorans KKPTGTLVVDNLLPGKTLWLLSTGTGLAPFBurkholderia_cenocepacia KKPTGTLIADNLLPGKTLWMLSTGTGLAPFBurkholderia_ambifaria KKPTGTLVADNLLPGKTLWMLSTGTGLAPFBurkholderia_vietnamiensis KKPTGTLVADNLLPGKTLWMLSTGTGLAPFBurkholderia_ubonensis KKPTGTLVADNLLPGKTLWMLSTGTGLAPFBurkholderia_thailandensis KKPTGTLVADNLLPGKTLWLLSTGTGLAPFBurkholderia_pseudomallei KKPTGTLVADNLLPGKTLWLLSTGTGLAPFBurkholderia_oklahomensis KKPTGTLVADNLLPGKTLWLLSTGTGLAPFHahella_chejuensis RKPTGTLVVDHLLPGRNLYLISTGTGLAPFReinekea_sp. SKPTGTLIVDNLLPGRNLYMISTGTGLAPFPseudomonas_putida KKPTGTLVLDDLNPGKHLYLLSTGTGLAPFPseudomonas_entomophila KKPTGTLVLDDLNPGKHLYLLSTGTGLAPFPseudomonas_fluorescens KKPTGTLVLDDLNPGKHLYLLSTGTGLAPFPseudomonas_mendocina KKPTGTLVLDDLNPGKHLYLLSTGTGLAPFRalstonia_eutropha KKPTGTLLVDNLLPGKTLWLLATGTGLAPFCupriavidus_taiwanensis KKPTGTLLVDNLLPGKTLWLLATGTGLAPFMethylobacterium_radiotolerans KKPTGTLVLDNLLPGRHLYLLGTGTGLAPFMethylobacterium_extorquens KKPTGTLVLDNLLPGKNLYLLGTGTGLAPFMethylobacterium_nodulans RKATGTLVLDNLLPGRHLYLLGTGTGLAPFXanthobacter_autotrophicus RKPTGTLVQDSLLPGKRLYLLSTGTGLAPFBrucella_melitensis KKPVGTLLYDNLKPGKHLWLLSTGTGLAPFBrucella_ovis KKPVGTLLYDNLKPGKHLWLLSTGTGLAPFBrucella_suis KKPVGTLLYDNLKPGKHLWLLSTGTGLAPFOchrobactrum_anthropi KKPVGTLLYDNLKPGKNLWLLSTGTGLAPFPsychrobacter_arcticus KKPTGTLVLDDLLPGKNLYMLSTGTGVAPFPsychrobacter_cryohalolentis KKPTGTLVLDDLLPGKNLYMLSTGTGVAPFPsychrobacter_sp. KKPTGTLVVDDLLPGKNLYMLSTGTGLAPFCaulobacter_crescentus KKPTGTLVLDAVRPGKRLFLFGTGTGLAPWconsensus * *** * * **** **

10

130. 140. 150.Oceanicaulis_alexandrii ASVIRDPETYERFDQVILTHTCREVAELTYMaricaulis_maris ASLIRDPETYEKFDQVILTHTCREAAELTYMesorhizobium_loti ASLLRDPDTYEKFDQLILTHTCRDNAELTYMesorhizobium_sp. ASLIRDPETYEKFEQVILTHTCRDVAELTYAurantimonas_sp. ASVIRDPETYEKFDQVILTQTCRGNAELAYFulvimarina_pelagi ASVIRDPETYEKFDQVILTQTCREDSELEYBartonella_quintana ASLIRDPETYEKFSEVVLIQTTRERDELTYBartonella_henselae ASLIRDPETYEKFSEVVLIQTTRECDELNYBartonella_tribocorum ASLIRDPDTYEKFSEVILIQTTREKNELTYBartonella_bacilliformis ASLIRDPETYEKFSQVVLIQTTRECNELTYHoeflea_phototrophica ASLVRDPETYDKFEEVILTHTCRDADELQYSinorhizobium_meliloti ASLIRDPETFEKFEEVILTHTCRDVAELKYSinorhizobium_medicae ASLIRDPETFEKFEEVILTHTCRDVAELKYAgrobacterium_tumefaciens ASLIRDPETYEKFEEVILTHTCRDVAELKYRhizobium_leguminosarum ASLIRDPETYEKFEEVILTHTTRDVAELKYRhizobium_etli ASLIRDPETYEKFEEVILTHTTRDVAELKYStappia_aggregata ASLIRDPDTYEKFDQVILTHTCREVAELKYHyphomonas_neptunium ASLVRDPDTYERYDEVIVTHTCRDVDELIYRhodobacter_sphaeroides ASLMRDPETYERYEQVIMMHTCREQAELEYParacoccus_denitrificans ASLMRDPETYERYEQVVMMHTCRTADELAYRhodobacter_capsulatus ASLMREPEAYEKFDEVIMMHACRTVAELEYJannaschia_sp. ASLLREPQTYEDYDEIIITHTCREVGELTYLoktanella_vestfoldensis ASLLREPQTYEDYDEIIITHTCRELGELTYDinoroseobacter_shibae ASLLREPQTYEDYDEVIITHTCREAGELDYPhaeobacter_gallaeciensis ASLLREPETYEKFDEVIITHTCREAGELTYSilicibacter_sp. ASLLREPQTYEDYDEVIITHTCREVGELTYSagittula_stellata ASLLREPQTYEDYDEVIITHTCREAAELHYOceanicola_batsensis ASLLRDPQTYEDYDEVIITHTCREVGELAYRoseovarius_sp. ASLLRDPQTYSDYDEVIITHTCRELGELQYRoseobacter_denitrificans ASLLRDPQTYEDYDEVIITHTCREVGELKYRoseobacter_litoralis ASLLRDPQTYEDYDEVIITHTCREVGELQYRoseovarius_nubinhibens ASLLREPQTYHDYDEVIITHTCRGADELKYSilicibacter_pomeroyi ASLLREPQTYEDYDEVIITHTCREVAELEYSulfitobacter_sp. ASLLREPETYEKFDQIIMTHTTRDVAELDYOceanibulbus_indolifex ASLLREPETYEKFDEVIVTHTCRDVAELEYRhodobacterales_bacterium ASLLRDPETYERFDQVIVTHTCRDVAELEYOceanicola_granulosus ASLLRDPETYEAYDEVIVTHTCRDVAELEYBurkholderia_dolosa MSIIRDPEIYDRFDKVVLTHTCRLKGELAYBurkholderia_multivorans MSIIRDPDIYDRFEKVVLTHTCRLKGELAYBurkholderia_cenocepacia MSIIRDPDIYDRFDKVILTHTCRLKGELAYBurkholderia_ambifaria MSIIRDPDIYDRFDKVILTHTCRLKGELAYBurkholderia_vietnamiensis MSIIRDPDIYERFDKVILTHTCRLKGELAYBurkholderia_ubonensis MSIIRDPDIYERFDKVILTHTCRLKGELAYBurkholderia_thailandensis MSIIRDPDIYERFDKVVLTHTCRLKGELAYBurkholderia_pseudomallei MSIIRDPDIYERFEKVVLTHTCRLKGELAYBurkholderia_oklahomensis MSIIRDPDIYERFDKVILTHTCRLKGELAYHahella_chejuensis MSIIKDPETYERFDKVILTHGVRYVSELAYReinekea_sp. MSVIKDPEVYEQYDKVILTHGVRTVDELAYPseudomonas_putida MSVIQDPETYERFEKVILVHGVRYVNEVAYPseudomonas_entomophila MSVIQDPETYERFEKVILVHGVRYVNEVAYPseudomonas_fluorescens MSVIQDPETYERFEKVILVHGVRYVNEVAYPseudomonas_mendocina MSVIQDPETYERFEKVILVHGVRYVNEVAYRalstonia_eutropha LSIIRDPEVYERYDKVVLTHTCRFVEELAYCupriavidus_taiwanensis LSIIRDPDVYERYDRVVLTHTCRFVEELAYMethylobacterium_radiotolerans LSIIKDPETYDRFEKVVLVHGCRQVQELAYMethylobacterium_extorquens LSIIKDPETYDRFEKVVLVHGCRQVQELAYMethylobacterium_nodulans LSIIKDPETYERFEKVVLVHGCRQVQELAYXanthobacter_autotrophicus LSVVKDPEAYERFEKVILIHGTRTVAELAYBrucella_melitensis LSIIRDLEVYERFEKVILVHGVRQVAELAYBrucella_ovis LSIIRDLEVYERFEKVILVHGVRQVAELAYBrucella_suis LSIIRDLEVYERFEKVILVHGVRQVAELAYOchrobactrum_anthropi LSIIRDLEAYERFEKIILVHGVRQVAELAYPsychrobacter_arcticus LALARDPEVYERFEKVILVHGVRGVSDLAYPsychrobacter_cryohalolentis LALARDPEVYERFEKVILVHGVRGISDLAYPsychrobacter_sp. LSLSRDPEVYEKFDKIILCHGVRKVEDLAYCaulobacter_crescentus LSVARDPDAYSRFERVIVAHGVREVKELAYconsensus * *

11

160. 170. 180.Oceanicaulis_alexandrii GKELVETVREDPLVGEFVG....DKLVYYPMaricaulis_maris GNELVAAVKDDELIGEFAR....EKLVHFAMesorhizobium_loti GQELVAALESDPLIGELTTG...R.VTLYNMesorhizobium_sp. GEELVAATREDPLIGEFTLG...R.LHHFTAurantimonas_sp. GRELVETSKADELMSEFIGD...K.LIFHAFulvimarina_pelagi GRQLVDHMKTDELMQEVVGE...KELLFHABartonella_quintana AKDLVDSLQQDPLIGEYV.....KQLKFYPBartonella_henselae AKDLVVSLQQDPLIGEYA.....PQLKFYPBartonella_tribocorum AKDLVCSLQQDPLIGTYA.....QQLKFYPBartonella_bacilliformis AKDLVASLQQDSLIGEYA.....QQLMFYPHoeflea_phototrophica GMDLVRECREDPLVGEFAG....DRLKHYASinorhizobium_meliloti GFDLVDEIRNHEFLNEIVG....DKLRHYASinorhizobium_medicae GFDLVDEIRNHEFLNEVVG....NKLRHYAAgrobacterium_tumefaciens GFDLVEEIRNHEFLNEIVG....DKLKHYARhizobium_leguminosarum GFDLVEEIQNDELLKEVVG....DKLRHYARhizobium_etli GFDLVHEIQNDELLKEVVG....DKLRHYAStappia_aggregata GEDLVQETINDPLIGEFAK....DKLVHYTHyphomonas_neptunium SRTLIDSLHNDPLVGEMVE....GKLKLYTRhodobacter_sphaeroides GRQLVESLKDDPLIGEMVG....DKLLYYPParacoccus_denitrificans GRELVENLRHDPLLGELYGEEFASRLLYYPRhodobacter_capsulatus GRQLVEALQEDPLIGELVEG....KLKYYPJannaschia_sp. GRDLIAALQHDELLNEVIGDGFWKKIKYYPLoktanella_vestfoldensis GRDLIEGLKTDELLNEVIGDGFWKKIKYYPDinoroseobacter_shibae GREVIESLKTDELLNEVIGDGFWKKIKYYPPhaeobacter_gallaeciensis GRELIESLKDDELLNEVIGEGFWKKIKYYPSilicibacter_sp. GRELIESLKEDELLNEVIGEGFWKKIKYYPSagittula_stellata GAELIEGLKTDELLNEVIGEGFWKKIKYYPOceanicola_batsensis GAELIDGIRKDELLNELIGADNLSKLRYYPRoseovarius_sp. GADLIESIRNDEMLAELIGEGFADKLRYYPRoseobacter_denitrificans GADLIESIKADELLNELIGEDNLKKLRYYPRoseobacter_litoralis GADLIESIKADELLNELIGEDYLKKLRYYPRoseovarius_nubinhibens GAALIDSIRNDEMLAELIGEGFADKIRYYPSilicibacter_pomeroyi GRQLIEEIRQDELLAELMGEGFADKIRYYPSulfitobacter_sp. GRTLVESLADDPLIGEMIG....DKLVYYPOceanibulbus_indolifex GRQLIEGLKSDELMQELIGTENLAKIRYYPRhodobacterales_bacterium GRQLVENLADDPLIGEFIG....NKLTYYPOceanicola_granulosus GRTLIDNLRADEMMQELLG.DGLDKLRYYPBurkholderia_dolosa MDYIKHDLPGHEYLGDIIK....EKLVYYPBurkholderia_multivorans MDYIKHDLPGHEYLGDIIK....EKLVYYPBurkholderia_cenocepacia MDYIKHDLPGHEYLGDIIK....EKLVYYPBurkholderia_ambifaria MDYIKHDLPGHEYLGDIIK....EKLVYYPBurkholderia_vietnamiensis MDYIKHDLPGHEYLGDIIK....EKLVYYPBurkholderia_ubonensis MDYIKHDLPGHEYLGDIIK....EKLVYYPBurkholderia_thailandensis MDYIKHDLPGHEYLGEVIR....EKLVYYPBurkholderia_pseudomallei MDYIKHDLPGHEYLGDVIR....EKLVYYPBurkholderia_oklahomensis MDFIKHDLPGHEYLGDVIR....EKLVYYPHahella_chejuensis QELIRDELPQNEFFGELVQ....EKLIYYPReinekea_sp. QDLIRDELPTNEYFGDLVR....EKLIYYPPseudomonas_putida REFITEHLPQNEFFGESVR....DKLIYYPPseudomonas_entomophila REFITEHLPQNEFFGESVR....DKLIYYPPseudomonas_fluorescens REFITEHLPQNEFFGEALR....DKLIYYPPseudomonas_mendocina REFITEHLPRNEFFGDALK....EKLIYYPRalstonia_eutropha RELIQEHLPQHEHLGDLVR....EKLVYFPCupriavidus_taiwanensis RELIQEHLPQHEHLGEMVR....EKLVYFPMethylobacterium_radiotolerans GETITEALPNHEFLGEMIA....AQLVYYPMethylobacterium_extorquens GETITETLPKHEFLGEMIA....NQLIYYPMethylobacterium_nodulans GETITQDLPNHELIGEMVR....TQLIYYPXanthobacter_autotrophicus DEFLTKELPNHEFLGDEVR....NKLIYYPBrucella_melitensis TDFISNELPQDEFLGEMVK....NQLIYYPBrucella_ovis TDFISNELPQDEFLGEMVK....NQLIYYPBrucella_suis TDFISNELPQDEFLGEMVK....NQLIYYPOchrobactrum_anthropi TDFIANELPQDEFLGEMVK....NQLIYYPPsychrobacter_arcticus RDMFENELPNDEIFGEDFR....KKFIYYPPsychrobacter_cryohalolentis RDMFENELPNDEIFGEDFR....KKFIYYPPsychrobacter_sp. REMFEETLPNDELFGEWYR....EKFIYYPCaulobacter_crescentus RDLFTQEIFDDPLVGDEAR....AQLTYYPconsensus

12

190. 200. 210.Oceanicaulis_alexandrii SVTREEFERKGRITQLMDDGVIYEELGIPPMaricaulis_maris SVTREEGPIKGRVTDMIESGELFERLGVPPMesorhizobium_loti STTREESARMGRITALIGSGKFYADLGIDKMesorhizobium_sp. STTREASDHVGRITTLVENGKLFQALGTEPAurantimonas_sp. SATRDGETRGHRITTLIENGKLFEDLAIAPFulvimarina_pelagi TATQKGEVTGDRVTKLIENGKLFIDLAIAPBartonella_quintana MTTRESSKHMGRITTVMESGAFFETTGLPKBartonella_henselae MTTRESSEHMGRITTVMESGAFFEMTGLPKBartonella_tribocorum MTTRESSEHMGRITTVMESGCFFETTGLPKBartonella_bacilliformis MTTREPSEHMGRITTVMKSGAFFEETGLPKHoeflea_phototrophica TCTRETYPFMGRITDLMASGKLFTDLGLPPSinorhizobium_meliloti TVTREEYPFKGRITDLMTNGKFFADLGLPPSinorhizobium_medicae TVTREDYSYKGRITDLMNNGKFFADLGLQPAgrobacterium_tumefaciens TVTREDYPFKGRITTLIENGKLFADLGVPARhizobium_leguminosarum TVTREDFEYRGRITDLISSGKLFTDLGVPPRhizobium_etli TVTREDFAYRGRITDLISSGKLFTDLGVPPStappia_aggregata SVTREDFPRQGRITDLIKSGKLFEDLGVPPHyphomonas_neptunium TTTREHYEHMGRITTLIENGKLFEDLGVPPRhodobacter_sphaeroides TTTRETSDRMGRITDNLSSGKVFEDLGVPKParacoccus_denitrificans TTTREETPYMGRITDNLTSGKVFADLNLPPRhodobacter_capsulatus TTTREEFHHMGRITDNLASGKVFEDLGIAPJannaschia_sp. TTTREESPKMGRITDLMRSGEAFSDLGVAPLoktanella_vestfoldensis TTTREDSPKMGRITDLMRSGEAFSDLGVPVDinoroseobacter_shibae TTTREQSPKMGRITDLMKSGECFSDLDVPVPhaeobacter_gallaeciensis TTTREESAKMGRITDLMRSGEAFADLGVPPSilicibacter_sp. TTTREESAKMGRITDLMRSGEAFADLGVPPSagittula_stellata TTTREESPKMGRITDLMRSGEAFRDLGVDPOceanicola_batsensis TTTREESPRMGRITDRMRSGEVFTDLGVEPRoseovarius_sp. TTTREESPKMGRITDLMRSGEVFADLGVAPRoseobacter_denitrificans TTTREESPKMGRITDLMRSGDVFADLGVAPRoseobacter_litoralis TTTREESPKMGRITDLMRSGDVFADLGVSPRoseovarius_nubinhibens TTTREESPKMGRITDLLRKGEVFEDLGIDPSilicibacter_pomeroyi TTTREESPRMGRITELLKDGTVFADLGIDGSulfitobacter_sp. TTTREQSPKMGRITNLLQDGTVFKDLGIDGOceanibulbus_indolifex TTTREESPKMGRITNLLQDGSVFKDLGVPQRhodobacterales_bacterium TTTREESPKMGRITTLLSDGTVFNDLGIETOceanicola_granulosus TTTREESPKMGRITTLIESAELFADLGVPABurkholderia_dolosa TVTREAFDNEGRITDLIASGKLFTDLGVA.Burkholderia_multivorans TVTREAFDNEGRITDLIASGKLFTDLGVA.Burkholderia_cenocepacia TVTREAFDNEGRITDLIATGKLFTDLGVP.Burkholderia_ambifaria TVTREAFDNEGRITDLIATGKLFTDLGVP.Burkholderia_vietnamiensis TVTREAFDNEGRITDLIATGKLFTDLGVA.Burkholderia_ubonensis TVTREEFENEGRITDLISTGKLFADLDVP.Burkholderia_thailandensis TVTREEFENEGRITDLIASGKLFADLDVP.Burkholderia_pseudomallei TVTREEFENEGRITDLIASGKLFTDLDVP.Burkholderia_oklahomensis TVTREEFENEGRITDLISSGKLFTDLDVP.Hahella_chejuensis TVTREDYPTQGRITDLMESGKLFSDLGLP.Reinekea_sp. TVTRESYENMGRLTELMENGKLFSDIGLP.Pseudomonas_putida TVTREPFENQGRLTDLMRSGKLFSDIGLP.Pseudomonas_entomophila TVTREPFENQGRLTDLMRSGKLFSDIGLP.Pseudomonas_fluorescens TVTREPFENQGRLTDLMRSGKLFSDIGLP.Pseudomonas_mendocina TVTREPFENQGRLTDLMRSGKLFADIGLP.Ralstonia_eutropha TVTREEFDNRGRITDLIASGELFERLGVE.Cupriavidus_taiwanensis TVTREEFDNRGRITDLIASGELFERLDMA.Methylobacterium_radiotolerans TVTREPFRNRGRITDLMVSGKLFEDIGLP.Methylobacterium_extorquens TVTREPFRNRGRITDLMTSGKLFEDIGLP.Methylobacterium_nodulans TVTREPFRNRGRITDLITSGKLFEDVGLP.Xanthobacter_autotrophicus TVTREPFRNQGRITDLITSGKLFADLGLP.Brucella_melitensis TVTREPYKNRGRLTDLIRSGQLFKDVGLP.Brucella_ovis TVTREPYKTRGRLTDLIRSGQLFKDVGLP.Brucella_suis TVTREPYKNRGRLTDLIRSGQLFKDVGLP.Ochrobactrum_anthropi TVTREPYKNRGRLTDLIRSGQLFTDIGLP.Psychrobacter_arcticus TVTREEFRNTGRITDLMKSGKFFEDIGLP.Psychrobacter_cryohalolentis TVTREEFRNTGRITDLMKSGKFFEDIGLP.Psychrobacter_sp. TVTREDFRNTGRITDLMRSGKLYEDIGLP.Caulobacter_crescentus TVTREAFERQGRFTDLITSGKLFQDLGIEGconsensus * * *

13

220. 230.Oceanicaulis_alexandrii ...LNPETDRVMICGSMDMLESCKQRCE.QMaricaulis_maris ...LDPESDRVMICGSEGLLRDVKQICL.DMesorhizobium_loti ...LNPETDRIMICGSMHMLKDVKELAE.SMesorhizobium_sp. ...FNPANDRIMICGSMAMLKDVKALAE.KAurantimonas_sp. ...LNPETDRAMICGSMAMLKDTEALLE.AFulvimarina_pelagi ...LDPANDRAMICGSMAMLKDTEKLLQ.EBartonella_quintana ...IHSNEDRVMICGSMAMLKDCARMCE.SBartonella_henselae ...IHADEDRVMICGSMAMLKDCARMCE.VBartonella_tribocorum ...IHPDEDRVMICGSMAMLKDCARMCE.SBartonella_bacilliformis ...INSDEDRVMICGSIEMIKDCAAMCE.DHoeflea_phototrophica ...LSPETDRGMICGSMEMLKDTRAALE.GSinorhizobium_meliloti ...LDPEIDRGMICGSTAMLKDTKEILE.ASinorhizobium_medicae ...LDPVIDRGMICGSTAMLKDTKEILE.AAgrobacterium_tumefaciens ...LDPAIDRGMICGSSAMLKDTKELLE.KRhizobium_leguminosarum ...LDPAIDRGMICGSSAMLKDTKELLE.KRhizobium_etli ...LDPAIDRGMICGSSAMLKDTKELLE.KStappia_aggregata ...LDPAVDRGMICGSMDMLKDTKALLE.EHyphomonas_neptunium ...LDPATDRAMICGSMEMIQDVKALML.KRhodobacter_sphaeroides ...MNLEEDRAMVCGSLQFNLDVKTVLE.SParacoccus_denitrificans ...MDAANDRAMICGSLAFNTDVKTVLE.GRhodobacter_capsulatus ...MNPETDRAMVCGSLAFNVDVMKVLE.SJannaschia_sp. ...LNAETDRAMICGNLAFNLELKDMFE.DLoktanella_vestfoldensis ...LSPGTDRAMICGNLAFNLELKEMLE.SDinoroseobacter_shibae ...LCPETDRAMICGNLAFNLELKDMLE.SPhaeobacter_gallaeciensis ...LNPESDRAMICGNLAFNLELKDLFENTSilicibacter_sp. ...LNPESDRAMICGNLAFNLELKDLFENTSagittula_stellata ...INPDTDRAMICGNLAFNLELKAMLE.EOceanicola_batsensis ...ISPENDRAMVCGNLAFNLEIKELLE.GRoseovarius_sp. ...LSPETDRAMVCGNLAFNLEIKDMLE.GRoseobacter_denitrificans ...MSPETDRAMVCGNLAFNLEIKDLLE.GRoseobacter_litoralis ...MSPETDRAMVCGNLAFNLEIKDLLE.GRoseovarius_nubinhibens ...IAPEHDRAMICGNLAFNLELKDLLE.ESilicibacter_pomeroyi ..GIKAETDRAMVCGSLAFNHDIKAILE.GSulfitobacter_sp. ...ISADTDRGMVCGSLEFNKDIKDVLE.GOceanibulbus_indolifex ...INATHDRAMVCGSLGFNKDIMEILE.GRhodobacterales_bacterium ...ITADTDRAMVCGSMGLNNDIKEILE.GOceanicola_granulosus ...LDPATDRAMVCGSLEFNKDIKALLE.ABurkholderia_dolosa .P.FSPENDRVMLCGSTAMLKDTTALLK.EBurkholderia_multivorans .P.FSPENDRVMLCGSTAMLKDTTALLK.QBurkholderia_cenocepacia .A.FSPENDRVMLCGSTAMLKDTTELLK.QBurkholderia_ambifaria .A.FSPENDRVMLCGSTAMLKDTTDLLK.QBurkholderia_vietnamiensis .P.FSPEHDRVMLCGSTAMLKDTTDLLK.QBurkholderia_ubonensis .P.FSPEHDRVMLCGSTAMLKDTTDLLK.QBurkholderia_thailandensis .P.FSPEHDRVMLCGSTAMLKDTTELLK.KBurkholderia_pseudomallei .P.FSPERDRVMLCGSTAMLKDTTELLK.KBurkholderia_oklahomensis .P.FSPEHDRVMLCGSTAMLKDTVELLK.KHahella_chejuensis .A.MDPEHDRFMVCGSPSMLKDTCSILD.SReinekea_sp. .D.MDPEHDRFMICGSPSMLKDTCKILD.APseudomonas_putida .P.INPQDDRAMICGSPSMLDETSEVLD.SPseudomonas_entomophila .P.INPQDDRAMICGSPSMLDETSEVLD.SPseudomonas_fluorescens .P.INPQDDRAMICGSPSMLDETSEVLD.SPseudomonas_mendocina .P.INPQDDRAMICGSPSMLDETSAVLD.SRalstonia_eutropha .P.FSLENDRIMLCGSPDMLKDVRAILE.ECupriavidus_taiwanensis .P.FSTENDRIMLCGSPDMLKDVRAILE.AMethylobacterium_radiotolerans .P.MSIEADRFMLCGSPDMIRDTRELLT.SMethylobacterium_extorquens .N.MSIENDRFMLCGSPEMIKDTREMLT.SMethylobacterium_nodulans .P.MTIEADRFMLCGSPDMIRDTRELLS.SXanthobacter_autotrophicus .V.ISPEEDRLMLCGSPQMLKDVVDLLE.SBrucella_melitensis .E.FNHEDDRMMLCGSPEMLAETKQILE.EBrucella_ovis .E.FNHEDDRMMLCGSPEMLAETKQILE.EBrucella_suis .E.FNHEDDRMMLCGSPEMLAETKQILE.EOchrobactrum_anthropi .E.FNHDDDRMMLCGSPEMLAETKQILE.EPsychrobacter_arcticus .P.MNKEDDRVLICGSMPFNAEVSAILD.DPsychrobacter_cryohalolentis .P.MNKEDDRVLICGSMPFNAEVSAILN.DPsychrobacter_sp. .P.INKEDDRVMICGSMPFNADISEILD.ACaulobacter_crescentus DR.FDPENDRAMLCGSMAMIKDTAALLE.Aconsensus ** **

14

240. 250.Oceanicaulis_alexandrii AGLK.EGSNARPAQYVVEKSFVG...Maricaulis_maris RNFV.EGSNAAPADFVVEKAFVAT..Mesorhizobium_loti LGFQ.EGSLSHPATFVVERAFVG...Mesorhizobium_sp. AGLK.EGSNAEPADFVVERAFVG...Aurantimonas_sp. RGFE.EGANNKPGTFVVERAFVD...Fulvimarina_pelagi RGFE.EGANNAPGTYVVERAFVD...Bartonella_quintana FGLV.EGANNAPATYVVERAFVG...Bartonella_henselae FGLV.EGANNAPATYVVERAFVG...Bartonella_tribocorum FGLV.EGANNAPATYVVERAFVE...Bartonella_bacilliformis FGLV.EGANNAPATYVVERAFVG...Hoeflea_phototrophica FGLE.EGANNRPSTFVVERAFVG...Sinorhizobium_meliloti AGLT.EGANNKPAEFVIERAFVG...Sinorhizobium_medicae AGLT.EGANNKPAEFVIERAFVG...Agrobacterium_tumefaciens AGLN.EGANNKPAEFVIERAFVG...Rhizobium_leguminosarum GGLN.EGANSKPAEFVIERAFVG...Rhizobium_etli AGLN.EGANSKPAEFVIERAFVG...Stappia_aggregata AGLT.EGANNKPAEFVVERAFVG...Hyphomonas_neptunium AGLT.EGSNAAPAEFVIEKAFAG...Rhodobacter_sphaeroides FGLR.EGANSEPLQYVVEKAFVGDGIParacoccus_denitrificans FGLR.EGANSDPKEFVVEKAFVGDGIRhodobacter_capsulatus YGLR.EGANSEPREFVVEKAFVGEGIJannaschia_sp. YGLE.EGANSKPAHYVVEKAFLD...Loktanella_vestfoldensis YGLN.EGANSNPQQYVVEKAFLD...Dinoroseobacter_shibae YGLE.EGANSKPAHYVVEKAFLD...Phaeobacter_gallaeciensis YGLE.EGANSKPAHFVVEKAFLD...Silicibacter_sp. YGLE.EGANSKPAHFVVEKAFLD...Sagittula_stellata YGLE.EGANSDPKQYVVEKAFLD...Oceanicola_batsensis YGLR.EGANSDPKEYVVEKAFVD...Roseovarius_sp. YGLR.EGANSDPKEYVVEKAFLD...Roseobacter_denitrificans YGLE.EGANSKPAQYVVEKAFLD...Roseobacter_litoralis YGLE.EGANSKPAQYVVEKAFLD...Roseovarius_nubinhibens FGLK.EGANSDPQTYVVEKAFLD...Silicibacter_pomeroyi FGLT.EGANSDPREFVIEKAFVG...Sulfitobacter_sp. FGLE.EGANSDPKHYVVEKAFVG...Oceanibulbus_indolifex FGLT.EGANSDPQHFVVEKAFVG...Rhodobacterales_bacterium FGLR.EGANSDPAEYVVEKAFVG...Oceanicola_granulosus HGLR.EGANSDPAEFVVEKAFVG...Burkholderia_dolosa AGLV.EGKNSAPGHYVIERAFVD...Burkholderia_multivorans AGLV.EGKNNAPGHYVIERAFVD...Burkholderia_cenocepacia AGLV.EGKNSAPGHYVIERAFVD...Burkholderia_ambifaria AGLV.EGKNSAPGHYVIERAFVD...Burkholderia_vietnamiensis AGLV.EGKNSAPGHYVIERAFVD...Burkholderia_ubonensis AGLV.EGKNSAPGHYVIERAFVD...Burkholderia_thailandensis AGLV.EGKNSAPGHYVIERAFVD...Burkholderia_pseudomallei AGLV.EGKNSAPGHYVIERAFVD...Burkholderia_oklahomensis AGLV.EGKNSAPGHYVIERAFVD...Hahella_chejuensis RGFT.EARQGDLGHYVIERAFVES..Reinekea_sp. RGFK.EAKQGELGHYVIERAFVES..Pseudomonas_putida FGLKISPRMREPGDYLIERAFVEK..Pseudomonas_entomophila FGLKISPRMREPGDYLIERAFVEK..Pseudomonas_fluorescens FGLKISARMREPGDYLIERAFVEK..Pseudomonas_mendocina FGLKISPRMREPGDYLIERAFVEK..Ralstonia_eutropha RGFA.EGNMSHPGHFVLEKAFVG...Cupriavidus_taiwanensis RGFA.EGNMSHPGHFVLEKAFVG...Methylobacterium_radiotolerans RGYE.EGNHGEAGHYVIEKAFVEK..Methylobacterium_extorquens LGYE.EGNHGEAAHYVIEKAFVEK..Methylobacterium_nodulans RGYI.EGNHGEAGHYVIEKAFVEK..Xanthobacter_autotrophicus RGFA.EGSQSAPGHYVIEKAFVER..Brucella_melitensis RGFT.EGSQSEPGEFVIEKAFVEK..Brucella_ovis RGFT.EGSQSEPGEFVIEKAFVEK..Brucella_suis RGFT.EGSQSEPGEFVIEKAFVKK..Ochrobactrum_anthropi RGFK.EGSQSEAGHYVIEKAFVEK..Psychrobacter_arcticus FGLTVSPRMGVQADYAVERAFVG...Psychrobacter_cryohalolentis FGLTVSPRMGVQADYAVERAFVG...Psychrobacter_sp. AGLTVSPRMGVPADYVVERAFVG...Caulobacter_crescentus QGLK.EGSNAEPGDFVIERAFVG...consensus * *

15

Alignment of Plant FNRs

10. 20. 30.Nostoc_commune ..MLERMYNQGAVEGAANTELG.SRIFLYEVVGLRNostoc_punctiforme ......MYNQGAVEGAANIELG.SRIFVYEVVGLRAnabaena ...................................Nodularia_spumigena ..MLERMYNQGAVEGAANRELG.SRVFVYEVVGLRArthrospira_platensis ......MYSPTGTGVAMRTGAA.GRIFVFEVEGMRLyngbya_sp. ......MYSPSGSGVATKTGAN.SRVFVFEVEGMRTrichodesmium_erythraeum MMEKQIMKTDMRLGVKTNTEYS.NRTFVYEVEGLRAcaryochloris_marina ...................................Thermosynechococcus_elongatus ......MYN........ATNSR.SRMFRYEVVGLRSynechocystis_sp. ......MYSPGYVATSSRQSDAGNRLFVYEVIGLSSynechococcus_sp. ......MYGITSTANSTGNQSYANRLFIYEVVGLGCyanothece_sp. ......MYSPSLVANSGGSSNNDNRLFVYEVVGLAMicrocystis_aeruginosa ......MYSPSTLAG........NRLFVYEVAGLNCrocosphaera_watsonii ......MYSSALGATSGQTTAYGSRMFVYEVVGLSPaulinella_chromatophora ...........MRVTTASYRQSDTRTFTLVVVGLSProchlorococcus_marinus ................MAFNQTEVVLGGIAHIPLVCapsicum_annuum .........................MATAVTAAVSNicotiana_tobaccum .........................MATAVSAAVSPisum_sativum .........................MAAAVTAAVSArabidopsis_thaliana .........................MSAAISAAVSSpinacia_oleracea ...................................Oryza_sativa .........................MAAVNTVSS.Triticum_aestivum .........................MAAQLTAAL.Zea .........................MAAVTAAAVSZea_mays .........................MATVMAAAV.Cyanidium_caldarium .........................MFAFLPSVASCyanophora_paradoxa .........................MAFVASVPVFconsensus

40. 50. 60.Nostoc_commune QSEETDQTNYPIRKSGSVFIRVP.....YNRMNQENostoc_punctiforme QGEETDQTNYPIRKSGSVFIRVP.....YNRMNQEAnabaena ...................................Nodularia_spumigena QNEETDQTNYPIRKSGSVFIRVP.....YNRMNQEArthrospira_platensis QGQNTDNFNYPIRRSGTVYLTVP.....YERMNQELyngbya_sp. QGGDSDKFTYPIRRSGRVSITVP.....YERMNQETrichodesmium_erythraeum QSQTAGELNNPIRRSGTVYITVP.....YSRMNQEAcaryochloris_marina ...................MEKP.....FDRLKKDThermosynechococcus_elongatus QTAETEKTNYAIRNSGSQFFNVP.....YDRMNQFSynechocystis_sp. QSTMTDGLDYPIRRSGSTFITVP.....LKRMNQESynechococcus_sp. GDGRNE..NSLVRKSGTTFITVP.....YARMNQECyanothece_sp. KNGNTDNLNYPVRQGGSVFITVP.....YGRMNQEMicrocystis_aeruginosa QNDNTDSLDYSIRQSGSVFFTVP.....YSRMNQECrocosphaera_watsonii QNTDNDSLDYPIRRSGSVFITVP.....YGRMNQEPaulinella_chromatophora IG.........KQRQAEKHFTVP.....FSQLQAFProchlorococcus_marinus IG.........AFYIVKALFYRG.....QDGELAPCapsicum_annuum ...LPSSKSTSFPTRTSIISPEK....INFNKVPLNicotiana_tobaccum ...LPSSKSTSFSSRTSIISTDK....INFNKVPLPisum_sativum ...LPYSNSTSLPIRTSIVAPER....LVFKKVSLArabidopsis_thaliana ...LPSSKSSSLLTKISSVSPQR....IFLKKSTVSpinacia_oleracea ...................................Oryza_sativa ...LPCSKAGAAVAGGAPR.PST..CSVFYPPRCWTriticum_aestivum ...PSYSPATTKAAAGGSS.PSS..HFLAYPSRPRZea LPSSSSSPAAAKAKASASASPSSPCGHLQFPRRHGZea_mays ...SSFPSSSAVVAKASPASPCA.....APHFRPRCyanidium_caldarium .....YRLQGVHAHGTCSVLGGK............Cyanophora_paradoxa AN..ASGLKTEAKVCQKPALKNS.....FFRGEEVconsensus

16

70. 80.Nostoc_commune MRRITRLGGTIVSIQPATALQP........VNGKANostoc_punctiforme MRRITRLGGTIVSIQPITALEP........VNGKAAnabaena ...................................Nodularia_spumigena MRRITRLGGKIVSIQAVSTLEQ........LNGKVArthrospira_platensis MRRLSKMGAKIVNIYPAGETPP........VRTQALyngbya_sp. MRRISRLGGRIVNIRPCNGNPS........AQSSATrichodesmium_erythraeum MRRITGMGGKILSIKTLNEHSE........ANSQKAcaryochloris_marina LRNFAKAGAKMVNIDVPEVEEK........PIIAEThermosynechococcus_elongatus MQQITRWGGKIVSIQPLNGTVA........PLAATSynechocystis_sp. MRRITRMGGKIVSIKPLEGDSPLPHTEGIAKPSQSSynechococcus_sp. MQRITKLGGKIVSIRPAED..........AAQIVSCyanothece_sp. MRRLTRMGAKIVSIKPLNGEIP........LQMTAMicrocystis_aeruginosa MRRITRLGGRIVSIKPFNGIAP........VATVSCrocosphaera_watsonii MRRISRMGGRIVSIQPVDAETP........LKSTSPaulinella_chromatophora VKLITSRGGKIKAVISSSDTNP.............Prochlorococcus_marinus FSFSYRQAAQRKSSSTTTSTEK.............Capsicum_annuum YYRNVSGGSKLVTIRAQVTTEA.............Nicotiana_tobaccum YYRNVSGGSRLVSIRAQVTTEA.............Pisum_sativum NNVSISG..RVGTIRAQVTTEA.............Arabidopsis_thaliana CYR......RVVSVKAQVTTDTT............Spinacia_oleracea ...................................Oryza_sativa SKRSSGNGVRAQASTTETTAAPA............Triticum_aestivum NVR...NGVRAQVSTTEPTAEPA............Zea GPR..AVRLRVQVSTTET...AE............Zea_mays AVR...AAIRAQASAVEAP................Cyanidium_caldarium .......ELRLLPSRVGCR................Cyanophora_paradoxa TSR...SFFASQAVSAKPATTFE............consensus

90. 100. 110. 120.Nostoc_commune SLGNATSEVSELATSGETANTEGNGKATPVN....Nostoc_punctiforme SFGNATSVVSELAKSGETANSEGNGKATPVN....Anabaena ...................................Nodularia_spumigena SHESAEHKEESLVTSDKAANTEGNGKATPVANSEVArthrospira_platensis APENGQQSQSSGTQTP...................Lyngbya_sp. TSGNGLQSQQSGEQKK...................Trichodesmium_erythraeum T....KTKQKTKPMTE...................Acaryochloris_marina P.....APEAPVAPPQ...................Thermosynechococcus_elongatus T........EPAANNG...................Synechocystis_sp. EGSGSEAVANPAPESNKT.................Synechococcus_sp. EGQSSAQASAQSPMASST.................Cyanothece_sp. S....QPTQQATKETEKGK................Microcystis_aeruginosa SASTPQPIAQSSPVPEQTK................Crocosphaera_watsonii NGSSTAKVAQP.QASNQTE................Paulinella_chromatophora .......RSESIRIPA...................Prochlorococcus_marinus .......PD..........................Capsicum_annuum ...................................Nicotiana_tobaccum ...................................Pisum_sativum ...................................Arabidopsis_thaliana ...................................Spinacia_oleracea ...................................Oryza_sativa ...................................Triticum_aestivum ...................................Zea ...................................Zea_mays ...................................Cyanidium_caldarium ...................................Cyanophora_paradoxa ...................................consensus

17

130. 140.Nostoc_commune ....AHSAEEQNK..DKKGNTMTQAKAKKDHGDVPNostoc_punctiforme ....AHSAEEQNK..DKKGNTMTQAKAKKDHGDVPAnabaena ......................TQAKAK..HADVPNodularia_spumigena KGFAKPPAEDQLKKKDKKGNTMTQAKAKK.GADVPArthrospira_platensis ....................TMTQAKAKT...DIPLyngbya_sp. ....................PMTQAKAHT...DVPTrichodesmium_erythraeum ....................AKPRRSEKK...SVPAcaryochloris_marina ....................SAKKAKKKD...DIPThermosynechococcus_elongatus ....................AAPVKEKKV...DIPSynechocystis_sp. .....................MTTTPKEKKADDIPSynechococcus_sp. .....................KIVHPKTTDTS.VPCyanothece_sp. .....................SMTQAKAKAKGDIPMicrocystis_aeruginosa .....................KKAMTQAKEKTDIPCrocosphaera_watsonii .....................KKRKSMTQAKTKIPPaulinella_chromatophora ....................TVTTSPAKPAHHDVPProchlorococcus_marinus ......................TKSP..PAQTAIPCapsicum_annuum ...................PAKVEKISKKQDEGVVNicotiana_tobaccum ...................PAKVEKISKKQDEGVIPisum_sativum ...................PAKVVKHSKKQDENIVArabidopsis_thaliana ................EAPPVKVVKESKKQEEGIVSpinacia_oleracea ...................................Oryza_sativa ................AEVTTKVEKVSKKQVDGVVTriticum_aestivum ................PAAPAKPVKISKKQDEGVVZea ................AEPVKKLEKVSKKQEEGLVZea_mays ..................ATAKAKKESKKQEEGVVCyanidium_caldarium ...................RGSATLHMVAAEKKVPCyanophora_paradoxa ................VDTTIRAQAVDAKKKGDIPconsensus

150. 160. 170. 180.Nostoc_commune VNTYRPNAPFIGKVISNEPLVKEGGIGIVQHLKFDNostoc_punctiforme VNTYRPNAPFIGKVISNEPLVKEGGIGIVQHLKFDAnabaena VNLYRPNAPFIGKVISNEPLVKEGGIGIVQHIKFDNodularia_spumigena VNIYRPNAPFVGKCISNEPLVKEDGIGIVQHLKFDArthrospira_platensis VNIYKPKNPYIGKCLSNEELVREGGTGTVRHLIFDLyngbya_sp. INIYKPKTPFTGKCLSSKELVQEGGEGTVRHLTFDTrichodesmium_erythraeum VNTYRPKNPFIGKCLSTAELVNEGGEGTVRHLVFDAcaryochloris_marina VNIYRPKTPFVGKCLSNEALVREGGEGRVQHLMFDThermosynechococcus_elongatus VNIYRPNNPCIGKVISNEELVREGGEGTVKHIIFDSynechocystis_sp. VNIYRPKTPYIGKVLENYPLVREGAIGTVQHLTFDSynechococcus_sp. VNIYRPKTPFLGKCIENYELVDEGGSGTVRHVTFDCyanothece_sp. VNIYRPNSPFIGKCVENYPLVGEGGSGIVQHLTFDMicrocystis_aeruginosa VNIYRPNQPFIGKCIENYALVDEGGIGIVQHVTFDCrocosphaera_watsonii TNIYRQKSPYVGKCIENYALVAEGGSGIVQHVTFDPaulinella_chromatophora VNLYKPKDPFVSTVIDNYSLLSPGAIGRVNHITFDProchlorococcus_marinus INLYKPKAPFEGTVLENYSLVKEGAVGRVNHITFDCapsicum_annuum VNKFRPKEPYIGRCLLNTKITGDDAPGETWHMVFSNicotiana_tobaccum VNKFRPKEPYVGRCLLNTKITGDDAPGETWHMVFSPisum_sativum VNKFKPKEPYVGRCLLNTKITGDDAPGETWHMVFSArabidopsis_thaliana VNKFKPKNPYTGRCLLNTKITGDDAPGETWHIVFTSpinacia_oleracea ...................................Oryza_sativa TNKYRPKEPYTGRCLLNTRITGDDAPGETWHMVFSTriticum_aestivum TNKYRPKEPYVGRCLLNTRLTGDNAPGETWHMVFSZea TNKYKPKEPYVGRCLLNTRITGDQAPGETWHMVFSZea_mays TNLYKPKEPYVGRCLLNTKITGDDAPGETWHMVFSCyanidium_caldarium VNLFKPSSPLVGTCIYNKKIVGDDAPGDTCHVIIHCyanophora_paradoxa LNLFRPANPYIGKCIYNERIVGEGAPGETKHIIFTconsensus

18

190. 200. 210.Nostoc_commune LSGSN..LKYIEGQSIGIIPPGLDKNGKPEKLRLYNostoc_punctiforme LSGGD..LKYIEGQSIGIIPPGLDKNGKPEKLRLYAnabaena LTGGN..LKYIEGQSIGIIPPGVDKNGKPEKLRLYNodularia_spumigena LSGGD..LKYVEGQSIGIIPPGVDKNGKPEKLRLYArthrospira_platensis ISGGD..LRYLEGQSIGIIPPGTDNNGKPHKLRLYLyngbya_sp. LSGGD..LRYLEGQSIGIIPPGTDANGKPHKLRLYTrichodesmium_erythraeum LSGSD..LHYLEGQSIGIIPPGKDAKGKAHKLRLYAcaryochloris_marina LSEGD..LRYIEGQSIGIIPAGTDANGKPHKLRLYThermosynechococcus_elongatus ISGTE..LRYLEGQSIGIIPAGTDANGKPHKLRLYSynechocystis_sp. LSAGD..LRYLEGQSIGIIPPGEDDKGKPHKLRLYSynechococcus_sp. ISEGD..LRYLEGQSIGIIPPGEDKNGKPHKLRLYCyanothece_sp. LSGGD..LHYVEGQSIGIIPPGTDDKGKPHKLRLYMicrocystis_aeruginosa LSGSD..LRYLEGQSIGIIPPGTDANGKPHKLRLYCrocosphaera_watsonii ISGGD..LHYLEGQSLAIIPPGTDAKGKPHKSRLYPaulinella_chromatophora LSGGN..LRYVEGQSIGIIPDGTDVNGKPHKLRLYProchlorococcus_marinus LAGSDPHLSYVEGQSIGIIPAGTDANGKPHKLRLYCapsicum_annuum TEGEIP...YREGQSIGVIADGVDANGKPHKLRLYNicotiana_tobaccum TEGEVP...YREGQSIGVIADGVDANGKPHKLRLYPisum_sativum TEGEVP...YREGQSIGIVPDGIDKNGKPHKLRLYArabidopsis_thaliana TEGEVP...YREGQSIGVIPEGIDKNGKPHKLRLYSpinacia_oleracea ..........REGQSVGVIPDGEDKNGKPHKLRLYOryza_sativa TDGEIP...YREGQSIGVIPDGIDKNGKPHKLRLYTriticum_aestivum TEGEVP...YREGQSIGVIADGEDKNGKPHKLRLYZea TEGEVP...YREGQSIGVIADGEDKNGKPHKLRLYZea_mays TEGKIP...YREGQSIGVIADGVDKNGKPHKVRLYCyanidium_caldarium HDGKLP...YLEGQSVGIIPEGTDDKGRPHKLRLYCyanophora_paradoxa HEGKVP...YLEGQSIGIIPPGTDKDGKPHKLRLYconsensus **** * * * * ***

220. 230. 240. 250.Nostoc_commune SIASTRHGDDVDDKTVSLCVRQLEYKHPETSETVYNostoc_punctiforme SIASTRHGDDVDDKTVSLCVRQLEYKHPETGETVYAnabaena SIASTRHGDDVDDKTISLCVRQLEYKHPESGETVYNodularia_spumigena SIASTRHGDDVDDKTVSLCVRQLEYKHPETGETVYArthrospira_platensis SIASTRHGDHVDDKTVSLCVRQLEYKHPETGETVYLyngbya_sp. SIASTRHGDNLDEKTVSLCVRQLEYKHPETGDTVYTrichodesmium_erythraeum SIASTRHGDHLNDQTVSLCVRRLEYNHPETGERIYAcaryochloris_marina SIASTRHGDRVDDKTISLCVRQLEYDHPETGERVYThermosynechococcus_elongatus SIASTRHGDFQDDKTVSLCVRRLEYKDKETGETIYSynechocystis_sp. SIASTRHGDFGDDKTVSLCVRQLEYQN.EAGETVQSynechococcus_sp. SIASTRHGDMEDNKTVSLCVRQLEYQDPESGETVYCyanothece_sp. SIASTRHGDKKDDKTVSLCVRQLEYEHPETKETVYMicrocystis_aeruginosa SIASTRHGDKLDDKTVSLCVRQLEYQHPETKETVYCrocosphaera_watsonii SIASTRHGDKVDDKTISLCVRQLEYEHPETKETVYPaulinella_chromatophora SIASSRHGDNLQGNTISLCVRQLQYEK..DGETINProchlorococcus_marinus SIASTRHGDNLEGKTVSLCVRQLQYEL..DGKTIDCapsicum_annuum SIASSALGDFGDSKTVSLCVKRLVYTN.DKGEEVKNicotiana_tobaccum STASSALGDFGDSKTVSLCVKRLVYTN.DKGEEVKPisum_sativum SIASSAIGDFGDSKTVSLCVKRLVYTN.DAGEVVKArabidopsis_thaliana SIASSAIGDFGDSKTVSLCVKRLVYTN.DGGEIVKSpinacia_oleracea SIASSALGDFGDAKSVSLCVKRLIYTN.DAGETIKOryza_sativa SIASSAIGDFADSKTVSLCVKRLVYTN.DQGEIVKTriticum_aestivum SIASSALGDFGDSKTVSLCVKRLVYTN.DAGEVVKZea SIASSALGDFGDSKTVSLCVKRLVYTN.DQGEVVKZea_mays SIASSAIGDFGDSKTVSLCVKRLIYTN.DAGEIVKCyanidium_caldarium SIASTAAGDFGDYKTLSLVVKRLVYTN.EKGEEVRCyanophora_paradoxa SIASTRHGDFGDDKTVSLSVKRLEYTD.ANGNLVKconsensus * ** ** ** * * *

19

260. 270. 280.Nostoc_commune GVCSTHLCFLKPG.EEVKITGPVGKEMLLPNDPDANostoc_punctiforme GVCSTHLCFLKPG.EEVKITGPVGKEMLLPNDPDAAnabaena GVCSTYLTHIEPG.SEVKITGPVGKEMLLPDDPEANodularia_spumigena GVCSTHLTQLEVG.AEVKITGPVGKEMLLPEDPEAArthrospira_platensis GVCSTYLCNLEAG.ADVAITGPVGKEMLLPEDEDALyngbya_sp. GVCSSFLCNIKEG.DDVSITGPVGKEMLLPDDEDATrichodesmium_erythraeum GVCSSYLCGMEEG.ADVAITGPVGKEMLLPDDEDAAcaryochloris_marina GVCSKHLCDMQPG.DDVKITGPVGKEMLLPDDPEAThermosynechococcus_elongatus GVCSSYLNQLQPG.DEVKITGPVGKEMLLSDDPEASynechocystis_sp. GVCSTYLCNIKEG.DDIAITGPVGKEMLLPPDEDASynechococcus_sp. GVCSTYLCNLPVGTDDVKITGPVGKEMLLPDDEDACyanothece_sp. GVCSTYLCHLEVG.ADVAITGPVGKEMLLPEDEDAMicrocystis_aeruginosa GVCSTHLCNVEVG.ADVAITGPVGKEMLLPGDEDACrocosphaera_watsonii GVCSTYLCNLEVG.ADVAMWGPVGKEMLLPDEEDAPaulinella_chromatophora GVCSTFLCDIKPG.AKTKMTGPVGKEMLLPADEMAProchlorococcus_marinus GVCSTYLCDIKPG.AKTKITGPVGKEMLLPDDEEACapsicum_annuum GVCSNFLCDLKPG.ADVKITGPVGKEMLMPKDPNANicotiana_tobaccum GVCSNFLCDLKPG.AEVKITGPVGKEMLMPKDPNAPisum_sativum GVCSNFLCDLKPG.SEVKITGPVGKEMLMPKDPNAArabidopsis_thaliana GVCSNFLCDLKPG.DEAKITGPVGKEMLMPKDPNASpinacia_oleracea GVCSNFLCDLKPG.AEVKLTGPVGKEMLMPKDPNAOryza_sativa GVCSNFLCDLKPG.SDVKITGPVGKEMLMPKDPNATriticum_aestivum GVCSNFLCDLKPG.SEVKITGPVGKKMLMPKDPNAZea GVCSNFLCDLKPG.AEVKITGPVGKEMLMPKDPNAZea_mays GVCSNFLCDLQPG.DNVQITGPVGKEMLMPKDPNACyanidium_caldarium GVCSNFLNDIKPG.EPIKMTGPVGKEMLMPDDPNACyanophora_paradoxa GVCSNYLCDLKPG.DEVMITGPVGTTMLMPEDQSAconsensus **** * * **** ** *

290. 300. 310. 320.Nostoc_commune NVIMMATGTGIAPMRAYLWRQFKDAERAANPEYQFNostoc_punctiforme NVIMMATGTGIAPMRAYLWRQFKDAERAANPEYQFAnabaena NVIMLATGTGIAPMRTYLWRMFKDAERAANPEYQFNodularia_spumigena KVIMMATGTGIAPMRAYLWRMFKDAERAANPEYQFArthrospira_platensis TIIMMATGTGIAPFRAFLWRIFK....EQHEDYKFLyngbya_sp. TIIMMATGTGIAPFRAFIWRMFKE..REQNPDYQFTrichodesmium_erythraeum TIIMIATGTGIAPYRAFLWRMFKE..REQNPDYQFAcaryochloris_marina NIIMMGTGTGIAPFRAYLWRMFK....EKHDDYKFThermosynechococcus_elongatus TIIMLATGTGIAPFRAFLWRMFK....ENNPDYQFSynechocystis_sp. NIVMLATGTGIAPFRAFLWRMFK....EQHEDYKFSynechococcus_sp. TVVMLATGTGIAPFRAFLWRMFK....EQHEDYKFCyanothece_sp. NIIMLATGTGIAPFRAFLWRMFK....EQHEDYKFMicrocystis_aeruginosa TIIMMATGTGIAPFRAFLWRMFK....EQHEDYKFCrocosphaera_watsonii NIIMLATGTGIAPFRSFLWRMFF....ENNPEYKFPaulinella_chromatophora NVIMLATGTGIAPMRTYLRRMFEPGERSKNPEYQFProchlorococcus_marinus NVIMLATGTGIAPMRAYLRRMFEPTERSKN.GWKFCapsicum_annuum TVIMLGTGTGIAPFRSFLWKMFF....EKHDDYKFNicotiana_tobaccum TVIMLATGTGIAPFRSFLWKMFF....EKHEDYKFPisum_sativum TVIMLGTGTGIAPFRSFLWKMFF....EKHEDYQFArabidopsis_thaliana TIIMLGTGTGIAPFRSFLWKMFF....EEHEDYKFSpinacia_oleracea TIIMLGTGTGIAPFRSFLWKMFF....EKHDDYKFOryza_sativa TIIMLGTGTGIAPFRSFLWKMFF....EEHDDYKFTriticum_aestivum TIIMLATGTGIAPFRSFLWKMFF....EEHEDYKFZea TIIMLATGTGIAPFRSFLWKMFF....EEHEDYKYZea_mays TIIMLATGTGIAPFRSFLWKMFF....EKHDDYKFCyanidium_caldarium TIIMLATGTGIAPFRAFMRKAFV....EKHADYQFCyanophora_paradoxa TIIMLATGTGIAPFRSFLRRMFE....ETHADYKFconsensus * ******* * *

20

330. 340. 350.Nostoc_commune KGFSWLIFGVPTTPNLLYKEELEEIQQKYPDNFRLNostoc_punctiforme KGFSWLIFGVPTTPNLLYKEELEEIQQKYPENFRLAnabaena KGFSWLVFGVPTTPNILYKEELEEIQQKYPDNFRLNodularia_spumigena KGFSWLIFGVPTTPNILYKEELEQMQEKYPDNFRLArthrospira_platensis KGLAWLFFGIPYSPNILYQQELEELQQQFPENFRLLyngbya_sp. KGLAWLFFGCAYTPNILYKEELEELQRQFPDNFRVTrichodesmium_erythraeum KGLAWLFFGCPYTPNILYKEELEELQREFPDNFRLAcaryochloris_marina KGLAWLFFGVAYTPNILYKEELEELQSQYPDNFRLThermosynechococcus_elongatus KGLAWLFFGVAYTANILYKDELEAIQAQYPDHFRLSynechocystis_sp. KGLAWLIFGIPKSENILYKDDLEKMAAEFPDNFRLSynechococcus_sp. KGKAWLIFGVPYTANILYKDDFEKMAAENPDNFRLCyanothece_sp. KGLAWLIFGVPYSANILYKEDLEEIEKTYPDNFRLMicrocystis_aeruginosa KGLAWLIFGVPKTANILYQEELEKIAAEFPDNFRLCrocosphaera_watsonii KGKSWLIFGVPYSANVLYKDQLEEIQTKYPDNFDLPaulinella_chromatophora RGKAWLFMGAPTTANLLYDDDFNRYQSEFPENFRYProchlorococcus_marinus RGKAWLFMGAPYTANLLYDEDFERYLREFPDNFIYCapsicum_annuum NGLAWLFLGVPTSSSLLYKEEFEKMKEKAPENFRLNicotiana_tobaccum NGTAWLFLGVPTSSSLLYKEEFEKMKEKAPENFRLPisum_sativum NGLAWLFLGVPTSSSLLYKEEFEKMKEKAPEXFRLArabidopsis_thaliana NGLAWLFLGVPTSSSLLYKEEFEKMKEKNPDNFRLSpinacia_oleracea NGLAWLFLGVPTSSSLLYKEEFEKMKEKAPDNFRLOryza_sativa NGLAWLFLGVPTSSTLLYREEFERMKEIAPERFRLTriticum_aestivum NGLAWLFLGVPTSDTLLYKEEFEKMVEIGGENFRLZea TGLAWLFLGVPTSDTLLYKEELEKMKEMAPDNFRLZea_mays NGLGWLFLGVPTSSSLLYKEEFGKMKERAPENFRVCyanidium_caldarium KGKMILYLGVPTSSSLLYRDELEEMKANFPDQVELCyanophora_paradoxa NGLAWLFLGVPTSSTLLYREELEKMQKANPNNFRLconsensus * * * **

360. 370. 380. 390.Nostoc_commune TAAISREQKNPQGGRMYIQDRVAEHADELWQLIKNNostoc_punctiforme TAAISREQKNPQGGRMYIQDRVAEHADELWQLIKNAnabaena TYAISREQKNPQGGRMYIQDRVAEHADQLWQLIKNNodularia_spumigena TCAISREQKNPQGGRMYIQDRVAEHADELWQLIKEArthrospira_platensis TLAISREQQNPEGGKMYIQDRIKENADQLWELIQKLyngbya_sp. TYAVSREQKNKDGGKMYIQHRIQENADELWQLNQKTrichodesmium_erythraeum TYAISREQKNKDGGKMYIQHRIQENAEELWQLIQKAcaryochloris_marina TYAISREQKAADGSKMYIQSRIAEHADELWELVQKThermosynechococcus_elongatus TYAISREQKTPDGGKMYIQGRIAEHADEIWQLLQKSynechocystis_sp. TYAISREQQNAEGGRMYIQHRVAENAEELWNLMQNSynechococcus_sp. TYAISREQKTADGGKVYVQSRVSEYADELFEMIQKCyanothece_sp. TYAISREQKNAEGGRMYIQHRVGEYAEELWKLMQNMicrocystis_aeruginosa TYAISREQQNPQGGRMYIQHRVAEHADEIWNLLQSCrocosphaera_watsonii TYAISREQKNSEGGRMYIQHRVAEQAEKLWTMLQDPaulinella_chromatophora TKAISREQQNTSGGRMYIQDRVTEHADEIFGMIEDProchlorococcus_marinus TKAISREQQNPKGGRMYIQDRVMEYADQIFKMIENCapsicum_annuum DFAVSREQTNEKGEKMYIQTRMAQYAEELWTLLKKNicotiana_tobaccum DFAVSREQTNEKGEKMYIQTRMAQYAEELWTLLQKPisum_sativum DFAVSREQVXDKGEXMYIXTRMAQYAXELWELLKKArabidopsis_thaliana DFAVSREQTNEKGEKMYIQTRMAEYAEELWELLKKSpinacia_oleracea DFAVSREQTNEKGEKMYIQTRMAQYAVELWEMLKKOryza_sativa DFAVSREQTNAAGEKMYIQTRMAEYKDELWELLKKTriticum_aestivum DFAVSREQTNAAGEKMYIQTRMAEYKEELWEMLKKZea DFAVSREQTNAAGEKMYIQTRMAEYKEELWELLKKZea_mays DYAVSREQTNAAGERMYIQTRMAEYKEELWELLKKCyanidium_caldarium HYAISREMKNKQGGRYYLQDSMAERGEEIWQLLRKCyanophora_paradoxa DYAISREQTDSKGEKMYIQNRIAEYANEFWNMIQKconsensus * *** * *

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400. 410. 420.Nostoc_commune EKTHTYICGLRGMEEGIDAALTAAAVKEGVTWSVYNostoc_punctiforme EKTHTYICGLRGMEEGIDAALTAAAAKEGVTWSDYAnabaena QKTHTYICGLRGMEEGIDAALSAAAAKEGVTWSDYNodularia_spumigena EKTHTYICGLRGMEGGIDEALTAAAAKEGVVWSDYArthrospira_platensis PNTHTYICGLKGMEGGIDEGMSAAAGKFDVDWSDYLyngbya_sp. PNTHTYICGLKGMEGGIDEGMSAAASKFGADWSEFTrichodesmium_erythraeum PNAHTYICGLKGMEDGIDEGMSAVTGQFDIDWGDYAcaryochloris_marina ENTHTYICGLKGMEGGIDEGMSAAAAKHGVDWSEYThermosynechococcus_elongatus KNTHVYMCGLRGMEPGIDEAMTAAAAKNGADWQEFSynechocystis_sp. PKTHTYMCGLKGMEPGIDEAFTALAEQNGKEWTTFSynechococcus_sp. PNTHVYMCGLKGMQPPIDETFTAEAEKRGLNWEEMCyanothece_sp. PKTHTYMCGLKGMEDGIDQALGAMAAQNGADWSEFMicrocystis_aeruginosa PKTHAYMCGLKGMEDGIDDAISGAAAKNGADWSVYCrocosphaera_watsonii PKTHLYMCGLKGMESGLEEGLRDFASKQGVEWPDFPaulinella_chromatophora SRTHVYMCGLRGMEPGIDQAMTAAAQAKGFNWAELProchlorococcus_marinus PKTHVYMCGLKGMEPGIDEAMTAAATAKGIDWSELCapsicum_annuum DNTFVYMCGLKGMEQGIDDIMSSLAAKEGIDWADYNicotiana_tobaccum DNTFIYMCGLKGMEQGIDEIMSALAERDGIVWADYPisum_sativum DXTFVYMCGLKGMEKGIDDIMVSLAAKDGIDWIEYArabidopsis_thaliana DNTFVYMCGLKGMEKGIDDIMVSLAAKDGIDWLEYSpinacia_oleracea DNTYVYMCGLKGMEKGIDDIMVSLAAAEGIDWIEYOryza_sativa DNTYVYMCGLKGMEKGIDDIMIDLAAKDGIDWLDYTriticum_aestivum DNTYVYMCGLKGMEKGIDDIMVDLAAKDGIDWIDYZea DNTYVYMCGLKGMEKGIDDIMLDLAAKDGINWLDYZea_mays DNTYVYMCGLKGMEKGIDDIMVSLAEKDGIDWFDYCyanidium_caldarium DNTYVYMCGLKGMDSGIDSFMTDLAAKDGVNWADFCyanophora_paradoxa PNTFVYMCGLRGMEDGIQQCMEDIAKANGTTWDAVconsensus * *** ** *

430. 440.Nostoc_commune QK.ELKKAGRWHVETYNostoc_punctiforme QK.QLKKAGRWHVETYAnabaena QK.DLKKAGRWHVETYNodularia_spumigena QK.QLKKAHRWHVETYArthrospira_platensis QK.QLKKKHRWHVETYLyngbya_sp. QK.QLKKDHRWHVETYTrichodesmium_erythraeum QK.KLKKEGRWHVETYAcaryochloris_marina QR.TLKKAHRWHVETYThermosynechococcus_elongatus LKGTLKKEGRWHVETYSynechocystis_sp. QR.EMKKEHRWHVETYSynechococcus_sp. RR.SMKKEHRWHVEVYCyanothece_sp. QK.QMKKEHRWHVETYMicrocystis_aeruginosa QK.QLKKEHRWHVETYCrocosphaera_watsonii VK.QLKKEHRWHVEVYPaulinella_chromatophora RP.QLKKAERWHVETYProchlorococcus_marinus RP.QLKKAHRWHVETYCapsicum_annuum KK.QLKKAEQWNVEVYNicotiana_tobaccum KK.QLKKAEQWNVEVYPisum_sativum KR.TLKKAEQWNVEVYArabidopsis_thaliana KK.QLKRSEQWNVEVYSpinacia_oleracea KR.QLKKAEQWNVEVYOryza_sativa KK.QLKKSEQWNVEVYTriticum_aestivum KK.QLKKAEQWNVEVYZea KK.QLKKSEQWNVEVYZea_mays KK.QLKRGDQWNVEVYCyanidium_caldarium KK.QLKQQHRYNVEVYCyanophora_paradoxa VK.GLKKEKRWHVETYconsensus * ** *

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Calculation of Standard Apparent Hydride Transfer Energy

The calculation of the apparent redox potentials of NADP and FAD is done as described in

CRC Handbook of Chemistry and Physics, David R. Lide Ed., 85th edition, 2004-2005, page

7-9, ”Standard Transformed Gibbs Energy of Formation For Imortant Biochemical Species” by

Petr Vanysek.

E′◦ = − 1

nF

(∆fG

′◦(red) − ∆fG′◦(ox)

)with n = 2 for two electron reductions and F = 9.6485 · 104Cmol−1. For I = 100 mM, pH

7.0, 298.15 K and 100 kPa the values of 1255.17 kJ/mol (FAD ox), 1297.43 kJ/mol (FAD red),

1008.7 kJ/mol (NADP ox) and 1070.97 kJ/mol (NADP red) are given. Using the above equa-

tion, standard apparent reduction potentials of E ′◦(FAD) = −219.00mV and E′◦(NADP ) =

−322.69mV can be obtained. Thus, the hydride transfer from FAD to NADP in aqueous solu-

tion is ∆E′◦ = 104mV which corresponds to an energy of 2.4kcal/mol.

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