measuring normalized areal density of volume fraction through image processing in imagej

$: Measuring normalized areal density of volume fraction through image processing in ImageJ$
Advanced Materials Processing & Testing Lab

Colorado State UniversityMechanical Engineering

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Trevor Aguirre

Areal density – Adjust contrast & brightnessOriginal image Contrast & Brightness adjusted



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Trevor Aguirre

Areal density – Binary operationsThreshold adjusted

Adjusts pixels to have values within threshold

Unconnected pixels “opened”Removes isolated pixels



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Trevor Aguirre

Areal density – Binary operation & AnalyzeHoles filled in Analyzed particles



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Trevor Aguirre

Areal density – Overlay and comparisonOverlay on original image Original image

98.54% density



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Trevor Aguirre

Areal density results

Sample Name Average Density from ImageJ

Standard Deviation of Density from

ImageJArchimedes Density

HA BNNT 015 98.48% 0.67% 92.72%HA BNNT 017 98.42% 0.90% 91.85%HA BNNT 021 95.96% 1.45% 93.78%



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Trevor Aguirre

1. Analyze Set scale1. Set Global scale for images captured at the same conditions

2. Image Duplicate1. Work with duplicated image to compare to original while working

3. Image Adjust Contrast/Brightness4. Image Adjust Threshold5. Process Binary Make Binary6. Process Binary Open7. Process Binary Fill Holes8. Analyze Set Measurements

1. Choose what calculations you want ImageJ to perform9. Analyze Analyze Particles

1. Display results2. Clear (previous) results3. Show: Outlines

Image J user guide

Areal density process

http://rsbweb.nih.gov/ij/docs/guide/146-29.html