droplet measurement using imagej

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Droplet measurement using imagej Sir this is one of the photo taken with rathod sir’s canon 12MP camera. I have transferred this photo in image j software.

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Post on 08-Apr-2015

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Page 1: Droplet Measurement Using Imagej

Droplet measurement using imagej

Sir this is one of the photo taken with rathod sir’s canon 12MP camera. I have transferred this photo in image j software.

Page 2: Droplet Measurement Using Imagej

Atomizer tip width is 3mm. I set the scale using this length as known length.

Page 3: Droplet Measurement Using Imagej

• Distance in pixel means total my picture is 4000*3000 pixels. Atomizer tip is 153.64 pixel. I have calibrated this to 3mm. And given unit of length as mm.

Page 4: Droplet Measurement Using Imagej

• Here I have zoomed my image to where the droplets are clear and less number. There is one option to subtract the back ground to remove any unwanted things in the back.

Page 5: Droplet Measurement Using Imagej

• Here we can see the difference in back ground. now the droplets are very clear to measure.

Page 6: Droplet Measurement Using Imagej

• Make binary option will recognize the droplets as they have

Page 7: Droplet Measurement Using Imagej

• Here we can select a rectangular box where we are interested in measurement

Page 8: Droplet Measurement Using Imagej

• I have selected one rectangular box, analyzed the particles

Page 9: Droplet Measurement Using Imagej

• Show out lines will give the outer lines and number to the droplets.

Page 10: Droplet Measurement Using Imagej

• It counts the number of droplets in the rectangular box only and has given number to the each droplet. Calculated the area of each droplet.

Page 11: Droplet Measurement Using Imagej

• Here the total number of droplets are 21 and area of each droplet is in mm. in toolbar we can save this data into excel directly.

Page 12: Droplet Measurement Using Imagej
Page 13: Droplet Measurement Using Imagej

• Here I have measured each droplet size by considering droplets as round in shape. Area=πd2/4. evaluated value of d for each droplet. And average droplet size is 0.151mm and 151um. We can cross check the values by directly measuring the droplet.

Page 14: Droplet Measurement Using Imagej

• Here I have taken direct measurement of one droplet that is numbered as 11 showing with arrow. The length is shown above in the image j window as 0.20mm. We can compare this with previous result in the excel

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Page 16: Droplet Measurement Using Imagej

• Accurate measurement is dependent on the image, if the photo is good means the droplets in round shape then measurement is easy with less error. Here I am showing one of the photo with very clear droplets. But here no scale (atomizer tip) to calibrate that’s why I have given other example.

• Sir this is my idea I don’t know whether it is fine or not. Please suggest me in this regard.