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newsAgrisearch

MAR 2011

Seed Quality Testing Service Now Offered

S E R V I C E S see page 4 see page 6

NIR grain analysers at Wagga and York offices

Agrisearch is excited to announce that it has extended its service capability to include seed quality testing. It has been an area that the company has long considered investing in to meet customer needs and is now proud to offer this service.

In order to meet our customer needs two near infrared (NIR) grain analysers have been purchased as well as other industry standard equipment to allow standard grain quality measurements. The Infratek NIR equipment supplied by FOSS is the same equipment used by bulk handlers Australia wide and globally. Such wide industry use and acceptance has allowed the technology of equipment and analysis calibrations to be refined to very high standards of reporting and repeatability.

The dedicated seed quality laboratories have been established at our existing Wagga Wagga and York (see article below) facilities to compliment other seed increase and packing activities currently undertaken. The table below shows standard tests that Agrisearch can offer clients with other crops and tests potentially being added on an as needs basis.

For any potential enquiries regarding testing capability or costing please contact Ian Francis at our Wagga Wagga office via email ian.francis@agrisearch.com.au or phone 02 6971 9085.

National Variety Trials — 2010 Western Australia2010 was a fairly tough season in the West and coincided with our first year of conducting variety trials for GRDC under the National Variety Trials (NVT) program, at the York and Katanning offices. In all, 24 trials of wheat, barley and canola were conducted at 16 sites in the South West. Considering the way the season ended up we were very happy with the outcomes that were achieved. Only one trial didn’t get through to harvest! It was also pleasing to note that most sites ended up with low CV’s and good yields for the season.

Where possible, sites were selected so that trials could be co-located with other trials. If confidentiality was not an issue, this gave clients and growers the opportunity to see and compare more than one trial.

NVT in 2011 for the York office is expected to be similar. The only major change that we hope to achieve

this year is to add a little more moisture into the equation.

Last year saw Agrisearch purchase an Infratek grain analysis unit for use at York. All staff have been trained in the use and operation of the unit. This service will be available to clients in 2011 for contract testing of grain specifically where timeliness of results is important.

For further information please contact Michael Lamond at our York office on 08 9641 2059 or at michael.lamond@agrisearch.com.au.

Crop*NIR testing Physical tests

moisture protein oil starch test weight screenings plump grain

Barley

Canola

Chickpeas

Corn

Faba beans

Field peas

Oats

Sorghum

Soybean

Triticale

Wheat

Wheat – Durum

Rice TBA

Seed and grain samples waiting for testing at York facility

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Animal Health at Agrisearch — What We Can Do!

Treating sheep in a field study

Animal Health research at Agrisearch has come a long way in the past 30 years. We have considerable experience in labo-ratory, pen and field studies. Our capabilities include Good Laboratory Practice (GLP) studies for target animal safety and tissue residues in sheep and cattle (can include urine and faeces), milk in cattle as well as residues in chickens. Efficacy studies are conducted to Good Clinical Practice (GCP) standards and we have experience in the following areas:

• Sheep: lice, sheep blowfly, nematodes

• Cattle: lice, buffalo fly, nematodes, ticks, nuisance fly, midge

• Horses: nematodes• Goats: nematodes and lice• Cats and dogs: fleas

(field only)

These studies are conducted both as large scale field studies or small scale paddock or pen studies. The research process can start with in-vitro insecticide screening, moving on to dose titration and confirmation studies and then finally into the larger scale on-farm field studies throughout Australia. Some not so common, but related studies include human exposure, ram fertility, and cattle growth promotant evaluations. We are also able to offer pharmacokinetic studies in large animals.

Animal Health StaffOur 18 experienced animal health staff can provide services Australia wide with animal health staff based in:

• WA — Katanning and York• SA — Eudunda,• VIC — Shepparton (access to Tasmania)• QLD — Innisfail, Bundaberg and Toowoomba• NSW — Narrabri, Orange and Wagga Wagga

Agrisearch is also able to offer collaborative projects with our sister company Agrivet Services, in their two offices in Havelock North and Christchurch in New Zealand.

Animal Health FacilitiesDetails of our facilities include:

Location Facilities available

Orange — NSW •30 outdoor pens (0.4 to 0.8 ha), capacity of 12 sheep per pen (3 sites)

•Indoor pens — capacity of 50 individual sheep or 6 pens of 8 sheep (1 site)

•Larger paddocks available for groups of >10 sheep or cattle

•An exposure house for sheep blowfly studies, a small sheep plunge dip

•Facilities for residue sampling of sheep, goats and cattle

•Access to a 10 pen (8 cattle per pen) dairy facility

Eudunda — SA •10 pen (0.4 ha) (10 sheep per pen) outdoor facility

•10 pen (6 sheep per pen) indoor facility

Shepparton — VIC •8 pen (8 sheep per pen) outdoor facility

•Access to dairy facilities

Innisfail — QLD 10 paddock (10 cattle per paddock) tropical dairy facility where we are able to run pen efficacy studies or tropical residue studies (meat or milk)

DocumentationWe have developed all documentation required for compliance to both GCP and GLP guidelines. Agrisearch has its own controlled documents for data recording (compiled into a notebook), SOPs, and study plan/protocol and report templates. Clients may also utilise their own templates and forms if this is the preferred option.

Cattle with Buffalo Fly in Far North QLD

Australian Sheep Blowfly (Lucillia cuprina)

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Agrisearch has its own Animal Care and Ethics Committee to meet all ACEC requirements. This committee is able to approve animal health work in all states, except SA, where we use the SA Government (PIRSA) committee. Meetings are held as required and not on set dates so that projects are not held up.

In-vitro Testing Services for Major External ParasitesAgrisearch offers in-vitro testing on many of the major external parasites of production and domestic animals. Testing to determine the LD50/LD90 can be conducted on field collected strains of the following parasites:• Australian sheep blowfly adult and

larva (all year)• Sheep body louse adult (all year) • Cattle biting louse adult (seasonal)• Longnosed cattle louse adult (seasonal)• Shortnosed cattle louse adult (seasonal)• Buffalo fly adult (seasonal)• Cattle tick adult (seasonal)• House fly adult (all year) • Cat flea adult (all year)• Brown dog tick adult (all year)• Australian paralysis tick adult (seasonal)

Various test methods include:• Direct contact such as micro-application• Treated filter paper• Treated food media (contact and feeding)

Test concentrations can be prepared from technical grade active constituents or formulated product.

Testing field populations of insects such as buffalo fly, nuisance flies (house fly) or cat fleas where resistance to certain insecticides is suspected can be conducted. This testing will verify the level of tolerance/resistance to an active constituent in a certain geographical area compared to other areas where the insecticide performs well.

Regulatory Affairs and Product DevelopmentAgrisearch’s regulatory affairs department has extensive experience with animal health applications to the APVMA,

from minor label changes to registration of products with novel active constituents. Agrisearch offers a complete registration management service which includes registration applications, label development and correspondence management with the APVMA. The company also has experience in dealing with the ACVM and ERMA in New Zealand. Full product development packages can be prepared in consultation with the client, our R&D staff, regulatory affairs and Agrisearch Analytical allowing for significant savings to be passed on to the client.

For further details please contact our newly appointed Principal Project Biologist Veronica Ingham (B.Ag.Sci. Hons, Ph.D.,12 years in R&D) at our Orange office on 02 6362 4539 or via email: veronica.ingham@agrisearch.com.au

Animal Care and Ethics

Sheep Worm ProjectWA Agrisearch staff member Meghan Cornelius was granted a scholarship at the beginning of 2010 to complete a Masters Degree through the Sheep CRC and Murdoch University. The project is focused on parasite control; looking at the relationship between body condition score, worm burdens and subsequent production loss in sheep.

With increasing resistance to all classes of drench occurring around Australia, new worm management strategies are being proposed to prolong the efficacy of current drenches. One such strategy is referred to as ‘targeted treatment’ which aims to treat only the proportion of sheep in a flock that will actually benefit from it, leaving the rest of the mob unexposed to the drench. The untreated sheep become a source of susceptible worms, that may breed with and dilute out the

resistant worms and slow down the development of drench resistance.

There has been considerable research into targeted treatment strategies all over Australia, however a suitable indicator for selecting the sheep that actually require drenching has not yet been identified. It has been a common assumption among farmers and advisors for years that sheep with a lower body condition score (BCS) will respond better to treatment then sheep with a high BCS, yet this has never been tested. This project is looking at how BCS of sheep affects (if at all) worm egg counts and subsequent loss of condition and production caused by worms that are known to cause scouring and reduced productivity. At the conclusion of this project we will know if sheep in a specific BCS (high or low) suffer more from worms compared to the other,

and therefore if BCS should be used by farmers to determine which sheep to leave untreated when using targeted treatment worm control.

The first year of trials has already been completed, with a second year of trials already planned and ready to go. The size of this project has already exceeded the scope of a Masters degree and in February 2011 approval was applied for to Murdoch University for an upgrade to PhD.

Weighing, condition scoring and drenching sheep at lamb marking at Woodanilling, WA

Exposure house for Sheep Blowfly Studies in Orange, NSW

In-vitro testing of Brown dog ticks (Rhipicephalus sanguineus)

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S E R V I C E S

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Expansion and diversity continues for Agrivet. Not only has there been an increase in staff, but both the horticultural and animal health sectors have taken on more varied projects. This has meant more office space being required to support the staff. Hopefully resource consent will be granted shortly and the additional three office complex is expected to be completed by April.

Forage brassicas growing in Canterbury

South IslandSean and the family have settled in well in Christchurch, although the earthquake in September last year and the over 5,000 subsequent shocks (even a recent one on 20 January measuring 5.1 on the Richter scale) has been quite unnerving. This year he has undertaken more work on broadacre crops, especially various insect pests of forage brassicas, as well as pipfruit in Nelson, and grapes in Marlborough and Waipara. PS Sean and family survived the recent earthquake unscathed.

Client visitsOver the quiter winter months Agrivet has always held one-on-one client visits in various parts of the country. Occasionally this also involves overseas clients. Gina visited Pfizer while in Europe in May last year. Andre visited the BASF research station in Limburgerhof, Germany in June and then the Syngenta research station at Stein, near Basel, Switzerland. He also met with senior research staff and the warm hospitality and frankness of discussions is greatly appreciated. In July Gina and Joseph visited several animal health companies in Melbourne and Sydney and then spent time at the Agrisearch Services headquarters in Orange.

What’s Happening!

Agrivet Services LtdPO Box 8734, 422 St George’s Road Havelock North 4157, New Zealandph 64 6 877 7017 • fax 64 6 877 7047

In April last year the National Animal Ethics Committee held their annual meeting in Napier. One of the reasons for holding meetings in the various regions is to visit local AEC committees. They expressed an interest in visiting Agrivet and so headed by the Chairperson, Dr Virginnia Williams, a group of 15 or so members came to Havelock North. After Andre gave a brief history of Agrivet time was spent discussing the challenges facing new AEC’s including the need for adequate training. The group travelled the 30 minutes to the animal research station and were very impressed with the setup and the level of professionalism of the whole organisation. It was an excellent opportunity to intermingle with veteran animal ethics people from a wide range of backgrounds. With Gina away overseas Joseph ably handled the range of questions relating to the various studies being undertaken.

NAEAC and Agrivet AEC get together in April 2010. NAEAC president Dr Virginia Williams second from left.

Animal Ethics AGM in Napier

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Forage brassica spraying application

Some of the studies undertaken require long and continuous time input at certain periods. Milk residue studies are a case in point and these are now undertaken regularly and go very smoothly.

The move into conducting trials with vaccines has been quite time consuming as staff come to grips with all the aspects involved with this type of research. More work is planned in this area, including a good potential for another artificial challenge study in late winter/early spring.

Another exciting study currently being undertaken for NZ Winegrowers is the level of agrichemical residues in sheep tissue where they are used in vineyards.

The practice of using sheep (of all ages) for leaf plucking is becoming more common in vineyards around the country. This practice is done to open up the area around the bunches to more light and aeration resulting in less disease and quicker ripening. It is a cheaper alternative to using manual or mechanical methods and much more environmentally friendly. Anecdotal evidence also suggests that sheep do a much better job than machines or people. However, little or no research has been conducted regarding the chemical residues present in the meat, edible offal and fat tissue of animals that have grazed vineyards.

An initial pilot study has been undertaken to determine the residue depletion profiles of commonly used fungicides, insecticides and herbicides in sheep in three Hawke’s Bay vineyards which have used a variety of agrichemicals. The results are keenly awaited by the industry, growers and agchem companies. The practice is not currently supported by agrichemical companies and NZ Food Safety Authority has recommended a six month withholding period for sheep grazing vineyards.

Leaf plucking, the New Zealand way.

Animal Studies — Leaf Plucking in Vineyards

GLP continues to feature prominently in both horticulture and animal health. Multi-site trials are now the norm in horticulture studies with usually three or more regions being involved. We are also involved in an international residue study. Communication and coordination are key aspects to the successful undertaking of these projects.

Under the watchful eye of Trevor Lupton, our lead QA

manager for a number of years, Terry Greig has become increasingly involved in auditing both horticulture and animal health studies. This has involved key on-site application and sampling audits of key personnel and now study plan and final reports. Acting as Monitor for animal health studies has also become part of his role, as well as involvement in process and facility audits. Terry is now almost full-time in this area.

GLP and QA News

Agrisearch, in partnership with Horticulture Australia, is leading the way in helping growers of vegetable brassicas to improve weed control methods, late in the crop growing cycle.

Weed control in broccoli, cauliflower and brussel sprouts is based almost entirely upon the extensive use of a range of pre emergence or very early post emergence herbicides. No alternatives for weed control later in the crop cycle, other than physical removal, are currently available.

The project — “Integrated Weed Management in Vegetable Brassicas’ has

investigated later post transplant herbicide application plus inter row applications of non-selective herbicides using shielded sprayers across eight sites over Australia. A full GLP residue program has also been completed with several candidates to enable full registration or permit applications to be made.

Agrisearch staff will use the results from this project along with current weed control strategies to develop an Australia wide grower resource package to assist farmers to maintain effective and sustainable weed control practices in these crops.

Agrisearch is the only CRO which has the capacity to complete, ‘in house’ all aspects of this type of project.

Improving Weed Control For Vegetable Brassica Growers

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Agrisearch Analytical Pty LtdLevel 1, 48 Victoria Road, Rozelle, NSW, 2039, Australia

Phone: 61 2 9810 3666 • Fax: 61 2 9810 3866contact@agrisearchanalytical.com.au

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Hamilton LaboratoryIn recent years Agrisearch Analytical has been providing a residues testing service to our New Zealand customers from our Rozelle laboratory.

In striving to provide the best service and value for our NZ customers it was decided that we must establish our own facility in NZ. We now can announce that we have opened a new laboratory in Hamilton. Hamilton, for non-Kiwis, is in the North Island and is close to the most productive areas of dairy, horticulture and vegetables in NZ.

Agrisearch Analytical’s Hamilton facility

The laboratory will initially cater for the needs of our customers in the regulatory area and will provide a GLP-recognised residues testing service in crop protection and animal health. In the future we will expand into product and multi-pesticide surveillance testing but at present these areas will still be concentrated in Australia. NZ Crop Protection and Animal Health companies are happy to have a choice of GLP accredited laboratories in NZ.

Andrew Westcott, Senior Project Chemist for New Zealand has been establishing the laboratory. Andrew relocated from Sydney to Hamilton late in 2010 with his family. Andrew and family are already feeling settled and part of the local community thanks to the friendly folk of Hamilton. Andrew has recently been joined in Hamilton by Project Chemist, SiyuanYang, a very experienced analyst and long-time resident of the North Island.

The Hamilton laboratory is located at 12–14 Pukete Rd, Hamilton and the phone number is (+64) 7 849 5109. The fax number is (+64) 7 849 6871.

If you have any queries regarding residue testing, GLP studies, or would like a quote for work in New Zealand please contact:

Andrew at andrew_westcott@agrisearchanalytical.com.au or

Ross at ross_shields@agrisearchanalytical.com.au or

Susan McKeon at susan_mckeon@agrisearchanaytical.com.au

New Zealand customers are invited to visit the laboratory by appointment.

Andrew Westcott, Senior Project Chemist in Hamilton, NZ

The Disappearing Zero & the Waters LC/MS/MSAnimal Health and Crop Protection companies continue to come under increasing scrutiny from Regulators such as the Australian Pesticides and Veterinary Medicines Authority (APVMA).

One area where this scrutiny is manifest is with the requirements for reporting residue trials and the continual lowering of detection limits. It is not that long since the

APVMA would be satisfied if the Limit of Reporting (LOR) for a residues trial was one-fifth of the Maximum Residue Limit (MRL). However, this is no longer the case and in a residue study that was recently completed by Agrisearch Analytical the LOR reported was 1/200 of the MRL. The scientific relevance of measuring such minute residues could be questioned but to no avail. Regulators everywhere require lower and lower reporting limits.

With this in mind Agrisearch Analytical has recently invested in the Waters Xevo TQS, the latest and most

Agrisearch Analytical Newsletter 2011

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sensitive LC-MS-MS instrument on the market. This may be hyperbole but in the opinion of Lan Byrnes our Technical Manager, this is one extremely sensitive mass spectrometer. Agrisearch Analytical will be in a good position to meet the Regulator’s demands for lower LOR’s for many years to come.

Technical Manager, Lan Byrnes optimising Xevo TQS parameters with Waters Australia Application Specialist, Brian Walker

Chemists as ChefsAgrisearch Analytical’s Christmas feast was prepared by our very own Masterchefs. With expert instruction from the Cheeky Food Group chefs we managed to produce our own spectacular — and delicious — Christmas feast.

Considering the experience our analysts have following complex methods of analysis it was amazing how they were taken out of their comfort zone when confronted with a simple recipe! After a champagne cocktail to calm the nerves everyone rose to the occasion. Beautiful dishes of salads, desserts and a wonderful Christmas ham were produced. One of the favourite dishes of the day was a fantastic flambé prawn dish. Our project chemist Fransiska Sanjaya amazed us all with her skills with a frying pan and a bottle of tequila. The recipe follows for those of you willing to try something new. Everyone enjoyed a wonderful lunch and raised a toast to the continuing success of Agrisearch Analytical in 2011.

Tequila Prawns with Corn & Avocado SalsaIngredients

2 ears of corn in the husk2 tbs. rice vinegar1 green capsicum, diced1 red capsicum, diced2 avocado, peeled and diced1 bunch fresh coriander, chopped (keep some for garnish)3 cloves garlic3–4 tsp. fresh lime juice1 tsp salt4–6 tbs. extra virgin olive oil

Method:1. Remove the husk and ‘silk’ threads from the corn.

Slice the corn kernels off the cob.

2. Place the kernels in a wide pan (NO OIL), Let it cook without stirring on a high heat for about 5 min, then stir only 2–3 more times till slightly charred. Put aside.

3. Roast the capsicums on till the skin is blackened and blistered). Remove and cover with plastic wrap to ‘sweat’ for about 10mins Peel, remove core and seeds, then dice.

4. Garlic — chop and crush to a paste. Set aside.

5. Mix all the ingredients well except avocado Then gently stir in avocado last Season with salt and pepper.

Prawns & Assembly:

Ingredients30 large prawns, peeled, tails on75 ml tequila (optional)3 cloves garlic, chopped4 green onions, finely sliced1 tsp (more or less) chilli flakesSalt & Pepper

1. Add olive oil to large heated sauté pan.2. Add the prawns & green onions & sauté.3. Add salt, pepper, garlic and chilli flakes then turn

the prawns.4. Flambé the pan with the Tequila:5. Add the corn & avocado salsa, stir gently and serve.6. Scatter with coriander.

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Fax this update to (02) 6362 7844

Orange 50 Leewood Drive, PO Box 972, Orange, NSW 2800 Telephone: (02) 6362 4539 • Fax: (02) 6362 7844 Email: martin.collett@agrisearch.com.auMartin Collett – Principal Project Biologist / Managing Director

Gosford 4/16 Jusfrute Drive,West Gosford, PO Box 9001 Wyoming, NSW 2250 Telephone: (02) 4322 8510 • Fax: (02) 4322 8471 Email: ian.ridley@agrisearch.com.auIan Ridley – Principal Project Biologist / Director

Narrabri 26 Wee Waa Road, PO Box 207, Narrabri, NSW 2390 Telephone: (02) 6792 4187 • Fax: (02) 6792 4608 Email: derek.litzow@agrisearch.com.auDerek Litzow – Principal Project Biologist / Director

Wagga Wagga Unit 2, 5 Sutton Street,Wagga Wagga, NSW 2650 Telephone: (02) 6971 9085 • Fax: (02) 6971 0932 Email: ian.francis@agrisearch.com.auIan Francis – Principal Project Biologist

Horsham 17a King Drive, Private Bag 260, Horsham, VIC 3401 Telephone: (03) 5382 7229 • Fax: (03) 5382 7609 Email: jeremy.white@agrisearch.com.auJeremy White – Project Biologist

Toowoomba 7 Eyers Street, PO Box 2581,Toowoomba, QLD 4350 Telephone: (07) 4634 7265 • Fax: (07) 4633 1913 Email: richard.holzknecht@agrisearch.com.auRichard Holzknecht – Senior Project Biologist

Agrisearch Analytical Pty LtdLevel 1, 48 Victoria Road, Rozelle, NSW, 2039, AustraliaTelephone: 61 2 9810 3666 • Fax: 61 2 9810 3866Email: ross_shields@agrisearchanalytical.com.auRoss Shields – Managing Director

Bundaberg 11/32 Wyllie Street, Bundaberg, QLD 4670 Telephone/Fax: (07) 4152 4294 Email: sara.pengelly@agrisearch.com.auSara Pengelly – Senior Project Biologist

Innisfail 1/35 Station Street, PO Box 1348, Innisfail, QLD 4860 Telephone: (07) 4061 7470 • Fax: (07) 4061 7470 Email: bill.farnsworth@agrisearch.com.auBill Farnsworth – Principal Project Biologist

Shepparton 5 Grant Court, PO Box 7387, Shepparton,Vic 3630 Telephone: (03) 5821 2021 • Fax: (03) 5831 1592 Email: les.mitchell@agrisearch.com.auLes Mitchell – Principal Project Biologist / Director

Melbourne 2 Parer Street, Resevoir,Vic 3073 Telephone: (03) 9460 2253 • Fax: (03) 9460 1388Email: marco.montagna@agrisearch.com.auMarco Montagna – Senior Project Biologist

Adelaide 16 Sunbeam Road, Glynde, SA 5070 Telephone: (08) 8365 7269 • Fax: (08) 8365 7266 Email: bill.frost@agrisearch.com.auBill Frost – Principal Project Biologist

York 5 Maxwell Street, PO Box 580, YORK, WA, 6302Telephone: (08) 9641 2059 • Fax: (08) 9641 2359 Email: michael.lamond@agrisearch.com.auMike Lamond – Principal Project Biologist / Director

Agrivet Services LtdPO Box 8734, 422 St George’s Road, Havelock North 4157, New ZealandTelephone: 64 6 877 7017 • Fax: 64 6 877 7047Email: andre@agrivet.co.nzAndre Geelen – Managing Director

Contacts

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