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Lecture 22: Genetic networks and the Lac Operon Reading: PBC Chapter 6.1.2 PBC Chapter 19

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Page 1: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,

Lecture 22:Genetic networks and the Lac Operon

Reading: PBC Chapter 6.1.2PBC Chapter 19

Page 2: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,

Recall:

Page 3: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 4: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 5: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 6: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 7: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,

Now what if we add regulation: repressors and

activators?

Page 8: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,

Repressors

Page 9: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,

TetR

IdeR

FadR

PurR

Examples of actual repressors

Page 10: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 11: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,

CAP

p53 tumor supressor

zinc finger DNA binding domain

leucine zipper DNA-binding domain

Examples of actual activators

Page 12: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 13: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 14: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 15: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 16: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 17: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,

The lac operon

Page 18: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 19: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 20: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 21: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,
Page 22: Lecture22 Genetic Networks Lac Operon - ManningGroup · 2018-11-27 · OPERON OFF because Lac repressor bound RNA polymerase OPERON ON mRNA Figure 4.15 Physical Biology of the Cell,

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