lecture 5 web: pollev.com/ucibio text: to: 37607 type in: 169964
TRANSCRIPT
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Lecture 5
Web: pollev.com/ucibio
Text: To: 37607Type in: 169964 <your
question>
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OK. What does this all have to do with enzymes?
Anfinsen’s experiment
Took a protein (AP) & forced it to unfold
- Protein lost activityTherefore:______________________________Allowed protein to recover
- Over time, activity returned
Therefore:______________________________
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What does a protein need in order to fold?
Protein folding information contained in
primary amino acid
sequence!OK. But how does amino acid sequence
“fold?”
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Peptide bond is planar…
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…but not bonds on either side!
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Rotation of bonds around peptide bond
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Peptide backbone rotation
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“Folding” proteins
Amino acids have different propertiesDifferent preferred Psi and Phi anglesBonds can rotate and pivot
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Secondary structure
3D structure
H-bonds between C=O & N-H
C=O & N-H of peptide bonds
Close together in primary structure
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Folding into secondary structures
Bond rotation causes secondary structurea-helix
b-sheet
Bends, Loops, Disordered
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The a-helix: Annotate
Different parameters define a-helix
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b-Sheet: Annotate
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b-Sheets
Sheets can be twisted
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Bends / Loops
Bends or Loops: ImportantStructured/Unstructured
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Tertiary structrue
Bring secondary structure elements together
Hydrophobic interactions important
Unstructured regions important
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The a-helix
Arrangement of side chains important
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Tertiary structure
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Tertiary structrue
Take picture
Upload to Dropbox assignment “Barrel structure”